Progress in Nuclear Magnetic Resonance Spectroscopy最新文献

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An overview of Helium-3 NMR: Recent developments and applications 氦-3核磁共振综述:最近的发展和应用
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-08-01 DOI: 10.1016/j.pnmrs.2023.08.001
Leonid B. Krivdin
{"title":"An overview of Helium-3 NMR: Recent developments and applications","authors":"Leonid B. Krivdin","doi":"10.1016/j.pnmrs.2023.08.001","DOIUrl":"10.1016/j.pnmrs.2023.08.001","url":null,"abstract":"<div><p>The present review is focused on experimental and theoretical methods together with applications of helium NMR in chemistry and biochemistry. It comprises two main sections, the first dealing with standardization and instrumentation for <sup>3</sup>He NMR spectroscopy and the second dealing with its practical applications, mainly those in general and organic chemistry with a special emphasis on the rapidly developing and exciting area of fullerenes encapsulating helium atoms. Several general applications of <sup>3</sup>He NMR spectroscopy in physical chemistry and biomedicine are also briefly discussed.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"136 ","pages":"Pages 83-109"},"PeriodicalIF":6.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Implementation and applications of shaped pulses in EPR 成形脉冲在EPR中的实现与应用
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-08-01 DOI: 10.1016/j.pnmrs.2023.04.003
Burkhard Endeward, Matthias Bretschneider, Paul Trenkler, Thomas F. Prisner
{"title":"Implementation and applications of shaped pulses in EPR","authors":"Burkhard Endeward,&nbsp;Matthias Bretschneider,&nbsp;Paul Trenkler,&nbsp;Thomas F. Prisner","doi":"10.1016/j.pnmrs.2023.04.003","DOIUrl":"10.1016/j.pnmrs.2023.04.003","url":null,"abstract":"<div><p>In this review, we describe the application of shaped pulses for EPR spectroscopy. Pulses generated by fast arbitrary waveform generators are mostly used in the field of EPR spectroscopy for broadband (200 MHz-1 GHz) excitation of paramagnetic species. The implementation and optimization of such broadband pulses in existing EPR spectrometers, often designed and optimized for short rectangular microwave pulses, is demanding. Therefore, a major part of this review will describe in detail the implementation, testing and optimization of shaped pulses in existing EPR spectrometers. Additionally, we review applications using such pulses for broadband inversion of longitudinal magnetization as well as for the creation and manipulation of transverse magnetization in the field of dipolar and hyperfine EPR spectroscopy. They demonstrate the great potential of shaped pulses to improve the performance of pulsed EPR experiments. We give a brief theoretical description of shaped pulses and their limitations, especially for adiabatic pulses, most often used in EPR. We believe that this review can on the one hand be of practical use to EPR groups starting to work with such pulses, and on the other hand give readers an overview of the state of the art of shaped pulse applications in EPR spectroscopy.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"136 ","pages":"Pages 61-82"},"PeriodicalIF":6.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Pure shift edited NMR methodologies for the extraction of Homo- and heteronuclear couplings with ultra-high resolution 纯移位编辑核磁共振方法的提取与超高分辨率的同核和异核耦合
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-08-01 DOI: 10.1016/j.pnmrs.2023.02.001
Sandeep Kumar Mishra , N. Suryaprakash
{"title":"Pure shift edited NMR methodologies for the extraction of Homo- and heteronuclear couplings with ultra-high resolution","authors":"Sandeep Kumar Mishra ,&nbsp;N. Suryaprakash","doi":"10.1016/j.pnmrs.2023.02.001","DOIUrl":"10.1016/j.pnmrs.2023.02.001","url":null,"abstract":"<div><p>The scalar couplings that result in the splitting of the signals in the NMR spectrum arise due to the interaction of the nuclear spins, whereby the spin polarization is transmitted through chemical bonds. The interaction strengths depend <em>inter alia</em> on the number of consecutive chemical bonds intervening between the two interacting spins and on the molecular conformation. The pairwise interaction of many spins in a molecule resulting in a complex spectrum poses a severe challenge to analyse the spectrum and hence the determination of magnitudes and signs of homo- and heteronuclear couplings. The problem is more severe in the analysis of <sup>1</sup>H spectra than the spectra of most of the other nuclei due to the often very small chemical shift dispersion. As a consequence, the straightforward analysis and the accurate extraction of the coupling constants from the <sup>1</sup>H spectrum of a complex spin system continues to remain a challenge, and often may be a formidable task. Over the years, the several pure shift-based one‐dimensional and two‐dimensional methodologies have been developed by workers in the field, which provide broadband homonuclear decoupling of proton spectra, removing the complexity but at the cost of the very informative scalar couplings. To circumvent this problem, several one‐dimensional and two‐dimensional NMR experiments have been developed for the determination of homonuclear and heteronuclear couplings (<em><sup>n</sup>J</em><sub>HX</sub>, where <em>n</em> = 1,2,3) while retaining the high resolution obtained by implementing pure shift strategies. This review attempts to summarize the extensive work reported by a large number of researchers over the years for the accurate determination of homo- and heteronuclear scalar couplings.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"136 ","pages":"Pages 1-60"},"PeriodicalIF":6.1,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10269221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Affinity measurement of strong ligands with NMR spectroscopy: Limitations and ways to overcome them 核磁共振波谱法测定强配体的亲和性:局限性和克服方法
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-07-18 DOI: 10.1016/j.pnmrs.2023.07.001
Claudio Dalvit , Isabel Gmür , Philip Rößler , Alvar D. Gossert
{"title":"Affinity measurement of strong ligands with NMR spectroscopy: Limitations and ways to overcome them","authors":"Claudio Dalvit ,&nbsp;Isabel Gmür ,&nbsp;Philip Rößler ,&nbsp;Alvar D. Gossert","doi":"10.1016/j.pnmrs.2023.07.001","DOIUrl":"10.1016/j.pnmrs.2023.07.001","url":null,"abstract":"<div><p>NMR spectroscopy is currently extensively used in binding assays for hit identification, but its use in dissociation constant determination is more limited when compared to other biophysical techniques, in particular for tight binders. Although NMR is quite suitable for measuring the binding strength of weak to medium affinity ligands with dissociation constant K<sub>D</sub> &gt; 1 μM, it has some limitations in the determination of the binding strength of tight binders (K<sub>D</sub> &lt; 1 μM). A theoretical analysis of the binding affinity determination of strong ligands using different types of NMR experiments is provided and practical guidelines are given for overcoming the limitations and for the proper set-up of the experiments. Some approaches require reagents with unique properties or highly specialized equipment, while others can be applied quite generally. We describe all approaches in detail, but give higher emphasis to the more general methods, like competition experiments, where we include actual experimental data and discuss the practical aspects.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"138 ","pages":"Pages 52-69"},"PeriodicalIF":6.1,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47965886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent technical developments and clinical research applications of sodium (23Na) MRI 钠(23Na) MRI的最新技术进展及临床研究应用
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-04-18 DOI: 10.1016/j.pnmrs.2023.04.002
Lena V. Gast , Tanja Platt , Armin M. Nagel , Teresa Gerhalter
{"title":"Recent technical developments and clinical research applications of sodium (23Na) MRI","authors":"Lena V. Gast ,&nbsp;Tanja Platt ,&nbsp;Armin M. Nagel ,&nbsp;Teresa Gerhalter","doi":"10.1016/j.pnmrs.2023.04.002","DOIUrl":"10.1016/j.pnmrs.2023.04.002","url":null,"abstract":"<div><p>Sodium is an essential ion that plays a central role in many physiological processes including the transmembrane electrochemical gradient and the maintenance of the body’s homeostasis. Due to the crucial role of sodium in the human body, the sodium nucleus is a promising candidate for non-invasively assessing (patho-)physiological changes. Almost 10 years ago, Madelin et al. provided a comprehensive review of methods and applications of sodium (<sup>23</sup>Na) MRI (Madelin et al., 2014) [<span>1</span>]. More recent review articles have focused mainly on specific applications of <sup>23</sup>Na MRI. For example, several articles covered <sup>23</sup>Na MRI applications for diseases such as osteoarthritis (Zbyn et al., 2016, Zaric et al., 2020) [<span>[2]</span>, <span>[3]</span>], multiple sclerosis (Petracca et al., 2016, Huhn et al., 2019) [<span>[4]</span>, <span>[5]</span>] and brain tumors (Schepkin, 2016) [<span>6</span>], or for imaging certain organs such as the kidneys (Zollner et al., 2016) [<span>7</span>], the brain (Shah et al., 2016, Thulborn et al., 2018) [<span>[8]</span>, <span>[9]</span>], and the heart (Bottomley, 2016) [<span>10</span>]. Other articles have reviewed technical developments such as radiofrequency (RF) coils for <sup>23</sup>Na MRI (Wiggins et al., 2016, Bangerter et al., 2016) [<span>[11]</span>, <span>[12]</span>], pulse sequences (Konstandin et al., 2014) [<span>13</span>], image reconstruction methods (Chen et al., 2021) [<span>14</span>], and interleaved/simultaneous imaging techniques (Lopez Kolkovsky et al., 2022) [<span>15</span>]. In addition, <sup>23</sup>Na MRI topics have been covered in review articles with broader topics such as multinuclear MRI or ultra-high-field MRI (Niesporek et al., 2019, Hu et al., 2019, Ladd et al., 2018) [<span>[16]</span>, <span>[17]</span>, <span>[18]</span>].</p><p>During the past decade, various research groups have continued working on technical improvements to sodium MRI and have investigated its potential to serve as a diagnostic and prognostic tool. Clinical research applications of <sup>23</sup>Na MRI have covered a broad spectrum of diseases, mainly focusing on the brain, cartilage, and skeletal muscle (see <span>Fig. 1</span>). In this article, we aim to provide a comprehensive summary of methodological and hardware developments, as well as a review of various clinical research applications of sodium (<sup>23</sup>Na) MRI in the last decade (i.e., published from the beginning of 2013 to the end of 2022).</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"138 ","pages":"Pages 1-51"},"PeriodicalIF":6.1,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47942952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electron paramagnetic resonance spectroscopy in structural-dynamic studies of large protein complexes 电子顺磁共振波谱在大型蛋白质复合物结构动力学研究中的应用
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-04-01 DOI: 10.1016/j.pnmrs.2022.11.001
Laura Galazzo, Enrica Bordignon
{"title":"Electron paramagnetic resonance spectroscopy in structural-dynamic studies of large protein complexes","authors":"Laura Galazzo,&nbsp;Enrica Bordignon","doi":"10.1016/j.pnmrs.2022.11.001","DOIUrl":"10.1016/j.pnmrs.2022.11.001","url":null,"abstract":"<div><p>Macromolecular protein assemblies are of fundamental importance for many processes inside the cell, as they perform complex functions and constitute central hubs where reactions occur. Generally, these assemblies undergo large conformational changes and cycle through different states that ultimately are connected to specific functions further regulated by additional small ligands or proteins. Unveiling the 3D structural details of these assemblies at atomic resolution, identifying the flexible parts of the complexes, and monitoring with high temporal resolution the dynamic interplay between different protein regions under physiological conditions is key to fully understanding their properties and to fostering biomedical applications.</p><p>In the last decade, we have seen remarkable advances in cryo-electron microscopy (EM) techniques, which deeply transformed our vision of structural biology, especially in the field of macromolecular assemblies. With cryo-EM, detailed 3D models of large macromolecular complexes in different conformational states became readily available at atomic resolution. Concomitantly, nuclear magnetic resonance (NMR) and electron paramagnetic resonance spectroscopy (EPR) have benefited from methodological innovations which also improved the quality of the information that can be achieved. Such enhanced sensitivity widened their applicability to macromolecular complexes in environments close to physiological conditions and opened a path towards in-cell applications.</p><p>In this review we will focus on the advantages and challenges of EPR techniques with an integrative approach towards a complete understanding of macromolecular structures and functions.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"134 ","pages":"Pages 1-19"},"PeriodicalIF":6.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9652345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Identifying disease progression in chronic kidney disease using proton magnetic resonance spectroscopy 利用质子磁共振波谱识别慢性肾病的疾病进展
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-04-01 DOI: 10.1016/j.pnmrs.2023.04.001
Tyrone L.R. Humphries , David A. Vesey , Graham J. Galloway , Glenda C. Gobe , Ross S. Francis
{"title":"Identifying disease progression in chronic kidney disease using proton magnetic resonance spectroscopy","authors":"Tyrone L.R. Humphries ,&nbsp;David A. Vesey ,&nbsp;Graham J. Galloway ,&nbsp;Glenda C. Gobe ,&nbsp;Ross S. Francis","doi":"10.1016/j.pnmrs.2023.04.001","DOIUrl":"10.1016/j.pnmrs.2023.04.001","url":null,"abstract":"<div><p>Chronic kidney disease (CKD) affects approximately 10% of the world population, higher still in some developing countries, and can cause irreversible kidney damage eventually leading to kidney failure requiring dialysis or kidney transplantation. However, not all patients with CKD will progress to this stage, and it is difficult to distinguish between progressors and non-progressors at the time of diagnosis. Current clinical practice involves monitoring estimated glomerular filtration rate and proteinuria to assess CKD trajectory over time; however, there remains a need for novel, validated methods that differentiate CKD progressors and non-progressors. Nuclear magnetic resonance techniques, including magnetic resonance spectroscopy and magnetic resonance imaging, have the potential to improve our understanding of CKD progression. Herein, we review the application of magnetic resonance spectroscopy both in preclinical and clinical settings to improve the diagnosis and surveillance of patients with CKD.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"134 ","pages":"Pages 52-64"},"PeriodicalIF":6.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10027734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanoscale quantum sensing with Nitrogen-Vacancy centers in nanodiamonds – A magnetic resonance perspective 纳米金刚石中氮空位中心的纳米量子传感——磁共振视角
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-04-01 DOI: 10.1016/j.pnmrs.2022.12.001
Takuya F. Segawa , Ryuji Igarashi
{"title":"Nanoscale quantum sensing with Nitrogen-Vacancy centers in nanodiamonds – A magnetic resonance perspective","authors":"Takuya F. Segawa ,&nbsp;Ryuji Igarashi","doi":"10.1016/j.pnmrs.2022.12.001","DOIUrl":"10.1016/j.pnmrs.2022.12.001","url":null,"abstract":"<div><p>Nanodiamonds containing fluorescent Nitrogen-Vacancy (NV) centers are the smallest single particles, of which a magnetic resonance spectrum can be recorded at room temperature using optically-detected magnetic resonance (ODMR). By recording spectral shift or changes in relaxation rates, various physical and chemical quantities can be measured such as the magnetic field, orientation, temperature, radical concentration, pH or even NMR. This turns NV-nanodiamonds into nanoscale quantum sensors, which can be read out by a sensitive fluorescence microscope equipped with an additional magnetic resonance upgrade. In this review, we introduce the field of ODMR spectroscopy of NV-nanodiamonds and how it can be used to sense different quantities. Thereby we highlight both, the pioneering contributions and the latest results (covered until 2021) with a focus on biological applications.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"134 ","pages":"Pages 20-38"},"PeriodicalIF":6.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9658456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Metabolic imaging with deuterium labeled substrates 氘标记底物的代谢成像
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2023-04-01 DOI: 10.1016/j.pnmrs.2023.02.002
Jacob Chen Ming Low, Alan J. Wright, Friederike Hesse, Jianbo Cao, Kevin M. Brindle
{"title":"Metabolic imaging with deuterium labeled substrates","authors":"Jacob Chen Ming Low,&nbsp;Alan J. Wright,&nbsp;Friederike Hesse,&nbsp;Jianbo Cao,&nbsp;Kevin M. Brindle","doi":"10.1016/j.pnmrs.2023.02.002","DOIUrl":"10.1016/j.pnmrs.2023.02.002","url":null,"abstract":"<div><p>Deuterium metabolic imaging (DMI) is an emerging clinically-applicable technique for the non-invasive investigation of tissue metabolism. The generally short T<sub>1</sub> values of <sup>2</sup>H-labeled metabolites <em>in vivo</em> can compensate for the relatively low sensitivity of detection by allowing rapid signal acquisition in the absence of significant signal saturation. Studies with deuterated substrates, including [6,6′-<sup>2</sup>H<sub>2</sub>]glucose, [<sup>2</sup>H<sub>3</sub>]acetate, [<sup>2</sup>H<sub>9</sub>]choline and [2,3-<sup>2</sup>H<sub>2</sub>]fumarate have demonstrated the considerable potential of DMI for imaging tissue metabolism and cell death <em>in vivo</em>. The technique is evaluated here in comparison with established metabolic imaging techniques, including PET measurements of 2-deoxy-2-[<sup>18</sup>F]fluoro-<span>d</span>-glucose (FDG) uptake and <sup>13</sup>C MR imaging of the metabolism of hyperpolarized <sup>13</sup>C-labeled substrates.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"134 ","pages":"Pages 39-51"},"PeriodicalIF":6.1,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10010956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
In-cell NMR: Why and how? 细胞内核磁共振:为什么?如何?
IF 6.1 2区 化学
Progress in Nuclear Magnetic Resonance Spectroscopy Pub Date : 2022-10-01 DOI: 10.1016/j.pnmrs.2022.04.002
Francois-Xavier Theillet , Enrico Luchinat
{"title":"In-cell NMR: Why and how?","authors":"Francois-Xavier Theillet ,&nbsp;Enrico Luchinat","doi":"10.1016/j.pnmrs.2022.04.002","DOIUrl":"10.1016/j.pnmrs.2022.04.002","url":null,"abstract":"<div><p>NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of data and ideas that emerged from NMR investigations on living cells. Covering a large proportion of the periodic table, NMR spectroscopy permits scrutiny of a great variety of atomic nuclei in all living organisms non-invasively. It has thus provided quantitative information on cellular atoms and their chemical environment, dynamics, or interactions. We will show that NMR studies have generated valuable knowledge on a vast array of cellular molecules and events, from water, salts, metabolites, cell walls, proteins, nucleic acids, drugs and drug targets, to pH, redox equilibria and chemical reactions. The characterization of such a multitude of objects at the atomic scale has thus shaped our mental representation of cellular life at multiple levels, together with major techniques like mass-spectrometry or microscopies.</p><p>NMR studies on cells has accompanied the developments of MRI and metabolomics, and various subfields have flourished, coined with appealing names: fluxomics, foodomics, MRI and MRS (i.e. imaging and localized spectroscopy of living tissues, respectively), whole-cell NMR, on-cell ligand-based NMR, systems NMR, cellular structural biology, in-cell NMR… All these have not grown separately, but rather by reinforcing each other like a braided trunk. Hence, we try here to provide an analytical account of a large ensemble of intricately linked approaches, whose integration has been and will be key to their success.</p><p>We present extensive overviews, firstly on the various types of information provided by NMR in a cellular environment (the “why”, oriented towards a broad readership), and secondly on the employed NMR techniques and setups (the “how”, where we discuss the past, current and future methods). Each subsection is constructed as a historical anthology, showing how the intrinsic properties of NMR spectroscopy and its developments structured the accessible knowledge on cellular phenomena. Using this systematic approach, we sought i) to make this review accessible to the broadest audience and ii) to highlight some early techniques that may find renewed interest. Finally, we present a brief discussion on what may be potential and desirable developments in the context of integrative studies in biology.</p></div>","PeriodicalId":20740,"journal":{"name":"Progress in Nuclear Magnetic Resonance Spectroscopy","volume":"132 ","pages":"Pages 1-112"},"PeriodicalIF":6.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0079656522000164/pdfft?md5=a0ef4cefbe87124a4623831d9e5ed0b0&pid=1-s2.0-S0079656522000164-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10789953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
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