Proceedings of the Japan Academy. Series B, Physical and Biological Sciences最新文献_第7页

Key contributions of a glycolipid to membrane protein integration. 糖脂对膜蛋白整合的关键贡献。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2024-01-01 DOI: 10.2183/pjab.100.026

Keiko Shimamoto, Kohki Fujikawa, Tsukiho Osawa, Shoko Mori, Kaoru Nomura, Ken-Ichi Nishiyama

{"title":"Key contributions of a glycolipid to membrane protein integration.","authors":"Keiko Shimamoto, Kohki Fujikawa, Tsukiho Osawa, Shoko Mori, Kaoru Nomura, Ken-Ichi Nishiyama","doi":"10.2183/pjab.100.026","DOIUrl":"10.2183/pjab.100.026","url":null,"abstract":"Regulation of membrane protein integration involves molecular devices such as Sec-translocons or the insertase YidC. We have identified an integration-promoting factor in the inner membrane of Escherichia coli called membrane protein integrase (MPIase). Structural analysis revealed that, despite its enzyme-like name, MPIase is a glycolipid with a long glycan comprising N-acetyl amino sugars, a pyrophosphate linker, and a diacylglycerol (DAG) anchor. Additionally, we found that DAG, a minor membrane component, blocks spontaneous integration. In this review, we demonstrate how they contribute to Sec-independent membrane protein integration in bacteria using a comprehensive approach including synthetic chemistry and biophysical analyses. DAG blocks unfavorable spontaneous integrations by suppressing mobility in the membrane core, whereas MPIase compensates for this. Moreover, MPIase plays critical roles in capturing a substrate protein to prevent its aggregation, attracting it to the membrane surface, facilitating its insertion into the membrane, and delivering it to other factors. The combination of DAG and MPIase efficiently regulates the integration of membrane proteins.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":"100 7","pages":"387-413"},"PeriodicalIF":4.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

At the dawn of novel aromatics: "On the Synthesis of Hinokitiol" by Tetsuo Nozoe et al. 新型芳烃的曙光Tetsuo Nozoe 等人撰写的 "论 Hinokitiol 的合成"。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-12-11 Epub Date: 2023-08-29 DOI: 10.2183/pjab.99.020

Hiroyuki Isobe

引用次数: 0

Synthesis and applications of helical polymers with dynamic and static memories of helicity. 具有螺旋度动态和静态记忆的螺旋聚合物的合成和应用。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-12-11 Epub Date: 2023-10-18 DOI: 10.2183/pjab.99.025

Eiji Yashima

引用次数: 0

Glancing at the birth of a galaxy of scientists from Rikō Majima. 看着星系诞生的科学家们从陆岛出发。

IF 3.1 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-11-10 Epub Date: 2023-08-29 DOI: 10.2183/pjab.99.019

Hiroyuki Isobe, Masahiro Hirama

引用次数: 0

Biomedical advances and future prospects of high-precision three-dimensional radiotherapy and four-dimensional radiotherapy. 高精度三维放射治疗和四维放射治疗的生物医学进展和未来展望。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-11-10 Epub Date: 2023-10-12 DOI: 10.2183/pjab.99.024

Hiroki Shirato

{"title":"Biomedical advances and future prospects of high-precision three-dimensional radiotherapy and four-dimensional radiotherapy.","authors":"Hiroki Shirato","doi":"10.2183/pjab.99.024","DOIUrl":"10.2183/pjab.99.024","url":null,"abstract":"Biomedical advances of external-beam radiotherapy (EBRT) with improvements in physical accuracy are reviewed. High-precision (±1 mm) three-dimensional radiotherapy (3DRT) can utilize respective therapeutic open doors in the tumor control probability curve and in the normal tissue complication probability curve instead of the one single therapeutic window in two-dimensional EBRT. High-precision 3DRT achieved higher tumor control and probable survival rates for patients with small peripheral lung and liver cancers. Four-dimensional radiotherapy (4DRT), which can reduce uncertainties in 3DRT due to organ motion by real-time (every 0.1-1 s) tumor-tracking and immediate (0.1-1 s) irradiation, have achieved reduced adverse effects for prostate and pancreatic tumors near the digestive tract and with similar or better tumor control. Particle beam therapy improved tumor control and probable survival for patients with large liver tumors. The clinical outcomes of locally advanced or multiple tumors located near serial-type organs can theoretically be improved further by integrating the 4DRT concept with particle beams.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"389-426"},"PeriodicalIF":4.4,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10749389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41210685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of patient care using cancer genomic profiling: SCRUM-/CIRCULATE-Japan experience. 使用癌症基因组图谱改善患者护理：SCRUM-/Circular-EJapan经验。

IF 3.1 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-10-11 Epub Date: 2023-08-09 DOI: 10.2183/pjab.99.015

Atsushi Ohtsu, Koichi Goto, Takayuki Yoshino

{"title":"Improvement of patient care using cancer genomic profiling: SCRUM-/CIRCULATE-Japan experience.","authors":"Atsushi Ohtsu, Koichi Goto, Takayuki Yoshino","doi":"10.2183/pjab.99.015","DOIUrl":"10.2183/pjab.99.015","url":null,"abstract":"We launched SCRUM-Japan platform for the cancer genome profiling (CGP) test screening followed by the enrollment to genomically-matched clinical trials in 2015. More than 30,000 tissue-based and 10,000 liquid-based CGP tests have already been performed for enrolling to a total of 127 industry-/investigator-initiated registration trials, which resulted in regulatory approvals of 12 new agents with 14 indications in Japan. Using the clinical-genomic database, a new driver gene was recently discovered with dramatic response by genomically-matched agent. Our comparative study with tissue-based CGPs revealed more usefulness of liquid biopsy in terms of less invasive manner, shorter turn-round time, and higher enrollment rate for matched treatments than tissue-based in gastrointestinal cancers. For detecting minimal/molecular residual disease (MRD) after surgery, post-surgical monitoring with tumor-informed liquid biopsy assay in association with two randomized control trials have also started in 2020 (CIRCULATE-Japan). The observational cohort study showed obvious efficacy of the MRD monitoring for predicting recurrence, leading to change clinical practice in patient selection who should receive adjuvant therapy in the near future.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"241-253"},"PeriodicalIF":3.1,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10749397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10020554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the occasion of the 100th volume of the Proceedings of the Japan Academy, Series B (PJA-B). 在《日本科学院院刊》B辑（PJA-B）第100卷之际。

IF 3.1 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-10-11 Epub Date: 2023-08-29 DOI: 10.2183/pjab.99.016

Kunihiko Suzuki, Shigekazu Nagata

引用次数: 0

Recent methodological advances towards single-cell proteomics. 单细胞蛋白质组学的最新方法进展。

IF 4.4 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-10-11 Epub Date: 2023-09-06 DOI: 10.2183/pjab.99.021

Sooyeon Kim, Latiefa Kamarulzaman, Yuichi Taniguchi

{"title":"Recent methodological advances towards single-cell proteomics.","authors":"Sooyeon Kim, Latiefa Kamarulzaman, Yuichi Taniguchi","doi":"10.2183/pjab.99.021","DOIUrl":"10.2183/pjab.99.021","url":null,"abstract":"Studying the central dogma at the single-cell level has gained increasing attention to reveal hidden cell lineages and functions that cannot be studied using traditional bulk analyses. Nonetheless, most single-cell studies exploiting genomic and transcriptomic levels fail to address information on proteins that are central to many important biological processes. Single-cell proteomics enables understanding of the functional status of individual cells and is particularly crucial when the specimen is composed of heterogeneous entities of cells. With the growing importance of this field, significant methodological advancements have emerged recently. These include miniaturized and automated sample preparation, multi-omics analyses, and combined analyses of multiple techniques such as mass spectrometry and microscopy. Moreover, artificial intelligence and single-molecule detection technologies have advanced throughput and improved sensitivity limitations, respectively, over conventional methods. In this review, we summarize cutting-edge methodologies for single-cell proteomics and relevant emerging technologies that have been reported in the last 5 years, and provide an outlook on this research field.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"306-327"},"PeriodicalIF":4.4,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10749393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10167486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly enantioselective hydroxymethylation of unmodified α-substituted aryl ketones in water. 未修饰的α-取代芳基酮在水中的高度对映选择性羟基甲基化。

IF 3.1 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-10-11 Epub Date: 2023-09-06 DOI: 10.2183/pjab.99.022

Taku Kitanosono, Tomoya Kawase, Yasuhiro Yamashita, Shū Kobayashi

{"title":"Highly enantioselective hydroxymethylation of unmodified α-substituted aryl ketones in water.","authors":"Taku Kitanosono, Tomoya Kawase, Yasuhiro Yamashita, Shū Kobayashi","doi":"10.2183/pjab.99.022","DOIUrl":"10.2183/pjab.99.022","url":null,"abstract":"Catalytic asymmetric direct-type aldol reactions of ketones with aldehydes are a perennial puzzle for organic chemists. Notwithstanding the emergence of a myriad of chiral catalysts to address the inherent reversibility of the aldol products, a general method to access acyclic α-chiral ketones from prochiral aryl ketones has remained an unmet synthetic challenge. The approach outlined herein is fundamentally different to that used in conventional catalysis, which typically commences with an α-proton abstraction by a Brønsted base. The use of a chiral 2,2'-bipyridine scandium complex enabled the hydroxymethylation of propiophenone to be run under base-free conditions, which avails effectual suppression of hydrolytic deactivation of the Lewis acid catalyst. Intriguingly, the use of water as a reaction medium had an overriding effect on the progress of the reaction. The sagacious selection of sodium dodecyl sulfate and lithium dodecyl sulfate as surfactants allowed a variety of propiophenone derivatives to react in a highly enantioselective manner.","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":" ","pages":"328-333"},"PeriodicalIF":3.1,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10749394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10167477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aromatic micelles: toward a third-generation of micelles. 芳香胶束:向第三代胶束发展。

IF 3.1 3区综合性期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2023-01-01 DOI: 10.2183/pjab.99.002

Michito Yoshizawa, Lorenzo Catti

引用次数: 1