Progress in Biomaterials最新文献

{"title":"Drug infused Al₂O₃-bioactive glass coatings toward the cure of orthopedic infection.","authors":"P Bargavi,&nbsp;R Riju Chandran,&nbsp;D Durgalakshmi,&nbsp;P Rajashree,&nbsp;R Ramya,&nbsp;S Balakumar","doi":"10.1007/s40204-022-00181-y","DOIUrl":"https://doi.org/10.1007/s40204-022-00181-y","url":null,"abstract":"<p><p>A unique implant coated substrate with dual-drug-eluting system exhibiting antibacterial, anti-inflammatory, and bone regenerative capacity has been fabricated using spray pyrolysis deposition (SPD) method. Bioglass (BG) and BG-alumina (BG-Al) composites coatings with different concentrations of Al incorporated on BG network over the Cp-Ti substrate were fabricated using SPD technique. Phase purity of BG and BG-Al composites were analyzed by XRD in which Na₂Ca₂Si₃O₉ and β-Na₂Ca₄(PO₄)₂SiO₄) and Na_7.15(Al_7.2Si_8.8O₃₂) phases were formed. Surface morphology of the coated substrates was analyzed by SEM. Uniformity of the coatings were evaluated by surface profilometer and the uniform distribution the nanoparticles were confirmed with Elemental mapping. Systematically, each apatite layer formation on coated substrate was confirmed by immersing the samples for 1, 3, and 7 days in simulated body fluid and the needle-like structure was characterized using SEM. Cumulative release of Tetracycline hydrochloride (Tet) antibiotic and Dexamethasone (Dex) anti-inflammatory drug-loaded BG-Al and BG-Al composite-coated substrate were studied for 24 h. Antibacterial activity of the coated substrates were evaluated by time-dependent growth inhibition and minimal inhibitory concentration (MIC) assays in which BG-Al and BG-Al composite loaded with Tet showed considerable growth inhibition against S. aureus. Osteoblast-like cells (MG-63) exhibited profound proliferation with no cytotoxic effects which was due to release of Dex drug-coated substrates. Thus, surface modification of Cp-Ti substrate with BG, BG-Al composites coatings loaded with Tet and Dex drug can be considered for post-operative orthopedic implant infection application.</p>","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"11 1","pages":"79-94"},"PeriodicalIF":4.9,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927578/pdf/40204_2022_Article_181.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39733197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microstructural and in-vitro characteristics of functional calcium silicate topcoat on hydroxyapatite coating for bio-implant applications.","authors":"Jarnail Singh,&nbsp;Sukhpal Singh Chatha,&nbsp;Hazoor Singh","doi":"10.1007/s40204-022-00183-w","DOIUrl":"https://doi.org/10.1007/s40204-022-00183-w","url":null,"abstract":"<p><p>The delayed tissue-implant interactions in metallic implants coated with hydroxyapatite (HA) paved the way for the development of alternative bioactive coatings. In this study, bi-layered functional gradient (HA-CS) coating was formulated by the atmospheric plasma spray (APS) process on Ti6Al4V alloy. The HA layer was applied at the metal interface to ensure long-term stability, while the calcium silicate (CS) outer layer was applied to achieve fast tissue-implant interactions. Moreover, single-layered HA and CS coating were also formulated for comparative analysis. The phase compositions, coating microstructure, chemical properties, microhardness, porosity, surface roughness, and in-vitro bioactivity were investigated. The CS top layer showed high porosity and surface roughness with respect to the inner HA layer, which constitutes an optimum microstructure to promote bioactivity. The microhardness of the outer CS layer of HA-CS was 520.3 ± 80.8 HV, while the corresponding value for the inner HA layer was 291.7 ± 45.7 HV. HA-CS and CS coatings demonstrated higher in-vitro bioactivity compared to HA coating. On the contrary, HA coating (3.76 mpy) displayed better corrosion resistance than the HA-CS (4.17 mpy) and CS coatings (4.34 mpy). The in-vitro results indicated that the HA-CS coating could promote the healthy development of osteoblast-like MG-63.</p>","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"11 1","pages":"95-108"},"PeriodicalIF":4.9,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927532/pdf/40204_2022_Article_183.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39942914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of current challenges and prospects of magnesium and its alloy for bone implant applications","authors":"Meysam Nasr Azadani, A. Zahedi, Oluwole K. Bowoto, Bankole I. Oladapo","doi":"10.1007/s40204-022-00182-x","DOIUrl":"https://doi.org/10.1007/s40204-022-00182-x","url":null,"abstract":"","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"39 1","pages":"1 - 26"},"PeriodicalIF":4.9,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73498204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Characteristics of a decellularized human ovarian tissue created by combined protocols and its interaction with human endometrial mesenchymal cells.","authors":"Maryam Nezhad Sistani,&nbsp;Saeed Zavareh,&nbsp;Mojtaba Rezazadeh Valojerdi,&nbsp;Mojdeh Salehnia","doi":"10.1007/s40204-021-00175-2","DOIUrl":"https://doi.org/10.1007/s40204-021-00175-2","url":null,"abstract":"","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"11 1","pages":"109"},"PeriodicalIF":4.9,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927483/pdf/40204_2021_Article_175.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39689566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial neural network for optimizing the formulation of curcumin-loaded liposomes from statistically designed experiments.","authors":"Ibilola M Cardoso-Daodu,&nbsp;Margaret O Ilomuanya,&nbsp;Andrew N Amenaghawon,&nbsp;Chukwuemeka P Azubuike","doi":"10.1007/s40204-022-00179-6","DOIUrl":"https://doi.org/10.1007/s40204-022-00179-6","url":null,"abstract":"<p><p>Curcumin is a primary polyphenol of the rhizomatous perennial plant called Curcuma Longa. Curcumin interferes favorably with the cellular events that take place in the inflammatory and proliferative stages of wound healing, hence its importance in skin regeneration and wound healing. Curcumin is however lipophilic, and this must be considered in the choice of its drug delivery system. Liposomes are spherical vesicles with bi-lipid layers. Liposomes can encapsulate both lipophilic and hydrophilic drugs, hence their suitability as an ideal drug delivery system for curcumin. There is, nevertheless, a tendency for liposomes to be unstable and have low encapsulation efficiency if it is not formulated properly. Formulation optimization of curcumin-loaded liposomes was studied by the application of artificial neural network (ANN) to improve encapsulation efficiency and flux of the liposomes. The input factors selected for optimization of the formulation were sonication time, hydration volume, and lipid/curcumin ratio. The response variables were encapsulation efficiency and flux. The maximum encapsulation efficiency and flux were obtained using lipid/curcumin ratio of 4.35, sonicator time of 15 min, and hydration volume of 25 mL. The maximum encapsulation efficiency and flux predicted were 100% and 51.23 µg/cm²/h, respectively. The experimental values were 99.934% and 51.229 µg/cm²/h, respectively. Curcumin-loaded liposome formulation is a promising drug delivery system in the pharmaceutical industry when formulated using optimized parameters derived from ANN statistically designed models.</p>","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"11 1","pages":"55-65"},"PeriodicalIF":4.9,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927551/pdf/40204_2022_Article_179.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39829338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Injectable hyaluronic acid-based antibacterial hydrogel adorned with biogenically synthesized AgNPs-decorated multi-walled carbon nanotubes.","authors":"Pooyan Makvandi,&nbsp;Milad Ashrafizadeh,&nbsp;Matineh Ghomi,&nbsp;Masoud Najafi,&nbsp;Hamid Heydari Sheikh Hossein,&nbsp;Ali Zarrabi,&nbsp;Virgilio Mattoli,&nbsp;Rajender S Varma","doi":"10.1007/s40204-021-00177-0","DOIUrl":"https://doi.org/10.1007/s40204-021-00177-0","url":null,"abstract":"","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"11 1","pages":"111"},"PeriodicalIF":4.9,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927476/pdf/40204_2021_Article_177.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39822669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fe₃O₄ nanoparticles coated with carboxymethyl chitosan containing curcumin in combination with hyperthermia induced apoptosis in breast cancer cells.","authors":"Negin Pazouki, Shiva Irani, Nafiseh Olov, Seyed Mohammad Atyabi, Shadab Bagheri-Khoulenjani","doi":"10.1007/s40204-021-00178-z","DOIUrl":"10.1007/s40204-021-00178-z","url":null,"abstract":"<p><p>Many studies have demonstrated that curcumin has potential anticancer properties. This research aims to study the effect of iron (II, III) oxide (Fe₃O₄) nanoparticles coated with carboxymethyl chitosan containing curcumin combination with hyperthermia on breast cancer cells. Magnetic nanoparticles coated with carboxymethyl chitosan containing curcumin (MNP-CMC-CUR) were prepared and specified. MCF-7, MDA-MB-231, and human fibroblast cells were treated with free curcumin and MNP-CMC-CUR at concentrations of 0-60 µM and at different time points. A combined therapy of MNP-CMC-CUR and hyperthermia was performed on MCF-7 cells. The cytotoxicity of curcumin and MNP-CMC-CUR combined with hyperthermia was assessed by MTT. The changes in TP53 and CASPASE3 gene expression were evaluated using real-time PCR. Both cell apoptosis and cell cycle were studied by Annexin/PI staining. The results of MTT showed that the IC₅₀ amount of MNP-CMC-CUR has significantly decreased compared to free curcumin (p < 0.05) and MNP-CMC-CUR in combination with the hyperthermia, and significantly reducing the metabolic activity of the cells (p < 0.05). Real-time PCR results revealed the up-regulation of TP53 and CASPASE3 (p < 0.05). The combinational therapy-induced cell apoptosis (64.51%) and sub-G1 cell cycle were arrested in MCF-7 cells. Based on these observations, a combination of MNP-CMC-CUR with hyperthermia could inhibit the proliferation of MCF-7 cells.</p>","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"11 1","pages":"43-54"},"PeriodicalIF":4.4,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927563/pdf/40204_2021_Article_178.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39817456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hyaluronic acid/PVA electrospun coating on 3D printed PLA scaffold for orthopedic application.","authors":"Mina Farsi,&nbsp;Azadeh Asefnejad,&nbsp;Hadi Baharifar","doi":"10.1007/s40204-022-00180-z","DOIUrl":"https://doi.org/10.1007/s40204-022-00180-z","url":null,"abstract":"<p><p>The need for bone tissue replacement, repair and regeneration for orthopedic application is constantly growing. Therefore, the application of cartilage substitute due to the lack of donors as well as biocompatibility leads to immune system rejection. In order to overcome these drawbacks, researchers have used porous scaffold as an option for bone transplantation. In this study, poly-lactic acid (PLA) scaffolds were prepared for cartilage application by fused deposition modeling (FDM) technique and then coated by electrospinning with polyvinyl alcohol (PVA) and hyaluronic acid (HLA) fibers. Hybrid electrospinning (ELS) method was used to produce porous scaffolds from HLA-PVA polymers. The printed scaffold was coated using FDM technique and the mechanical and biological investigation was performed on the polymeric composite specimen. The functional group and morphological behavior were investigated using Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) techniques. The obtained porous scaffold has hydrophilic properties as the PVA and HLA were coated on the PLA. The porous 3D-printed scaffold containing PLA/PVA/HLA scaffold does not show any toxicity in MTT evaluation after 1, 3 and 7 days. The SEM image confirmed the cell adhesion of the chondrite to the scaffold. Also, the mechanical performances of the sample, such as elastic modulus and compressive strength, were evaluated by compression test. By electro-spun coating, the elastic module of PVA/PLA and PLA/PVA/HLA scaffolds has increased to 18.31 ± 0.29 MPa and 19.25 ± 0.38 MPa. Also, the tensile strength of these two porous scaffolds has reached 6.11 ± 0.42 MPa and 6.56 ± 0.14 MPa, respectively. The failure strain of 3D printed PLA scaffold was reported to be 53 ± 0.21% and this value was reduced to 47 ± 0.62% and 42 ± 0.22% in PVA/PLA and PLA/PVA/HLA scaffolds. The cells' growth on the porous scaffolds showed a broad, spindle-shaped and regular shape. The obtained results of the chemical, physical and biological analyses showed that porous PLA/PVA/HLA scaffold has potential applications in cartilage construction.</p>","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"11 1","pages":"67-77"},"PeriodicalIF":4.9,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927516/pdf/40204_2022_Article_180.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39938337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design expert assisted formulation, characterization and optimization of microemulsion based solid lipid nanoparticles of repaglinide.","authors":"Balaji Maddiboyina,&nbsp;Vikas Jhawat,&nbsp;Ramya Krishna Nakkala,&nbsp;Prasanna Kumar Desu,&nbsp;Sivaraman Gandhi","doi":"10.1007/s40204-021-00174-3","DOIUrl":"https://doi.org/10.1007/s40204-021-00174-3","url":null,"abstract":"<p><p>Repaglinide, a member of the meglitinide class of drugs, is a new anti-diabetic agent that is utilized as an oral hypoglycemic agent. Using glyceryl monostearate, cetyl palmitate, and tristearin as lipids and poloxamer 188 as a surfactant, repaglinide-loaded solid lipid nanoparticles were created. Solid lipid nanoparticles were prepared utilizing an o/w microemulsion technique, which included the lipids glyceryl monostearate and tristearin, as well as waxes such as cetyl palmitate and the surfactant poloxamer 188. The mean particle size of the solid lipid nanoparticles formed was around 339 nm, with an entrapment efficiency of 82.20%. In-vitro release studies continued to be conducted using the dialysis bag diffusion technique. Within 12 h, the cumulative drug release was 88.4%. The results indicate that repaglinide was released more slowly from solid lipid nanoparticles made from tristearin and glyceryl monostearate in an equal ratio. Tristearin found the controlled release and extreme entrapment from other lipid carriers like glyceryl monostearate and cetyl palmitate. Differential scanning calorimetry demonstrates that repaglinide is entangled in amorphous or molecular state within solid lipid nanoparticles. SEM microscopy revealed that the produced repaglinide solid lipid nanoparticles had a spherical shape. After one month of storage at 2-8 °C, short-term stability testing revealed no significant alteration.</p>","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"10 4","pages":"309-320"},"PeriodicalIF":4.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633183/pdf/40204_2021_Article_174.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39651368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of titanium dioxide intermediate layer on scratch and corrosion resistance of sol-gel-derived HA coating applied on Ti-6Al-4V substrate.","authors":"Rezvan Azari,&nbsp;Hamid Reza Rezaie,&nbsp;Alireza Khavandi","doi":"10.1007/s40204-021-00169-0","DOIUrl":"https://doi.org/10.1007/s40204-021-00169-0","url":null,"abstract":"<p><p>Modification of dental and orthopedic implants' surface by coating them with bioactive materials, such as hydroxyapatite (HA), diminishes the implants' fixation time. Appropriate adhesion to the substrate and stability in biological conditions are essential requirements for these coatings. In this study, sol-gel-derived HA coating was applied on the Ti-6Al-4 V substrate, which is a high-performance alloy for manufacturing bone implants. Also, titanium dioxide (TiO₂) which was prepared by the sol-gel method was used as an intermediate layer between HA coating and the substrate. The nano-scratch and potentiodynamic polarization tests were employed to evaluate the effectiveness of TiO₂ intermediate layer on improving the scratch resistance, as an indicator of coating adhesion strength, and the corrosion resistance of the coated samples. The quality of the coating bonded to the substrate was studied by cross-sectional SEM images. The XRD tests indicated that HA and TiO₂ coatings were formed with predetermined phase compositions. The biocompatibility of sol-gel-derived HA coating was established by simulated body fluid (SBF) immersion tests. The SEM images, along with the results of electrochemical and nano-scratch tests, proved the significant effect of a TiO₂ intermediate layer on improving the scratch resistance and stability of HA coating on titanium alloy substrate.</p>","PeriodicalId":20691,"journal":{"name":"Progress in Biomaterials","volume":"10 4","pages":"259-269"},"PeriodicalIF":4.9,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633272/pdf/40204_2021_Article_169.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39683408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}