Drug infused Al2O3-bioactive glass coatings toward the cure of orthopedic infection.

IF 4.4 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Progress in Biomaterials Pub Date : 2022-03-01 Epub Date: 2022-01-30 DOI:10.1007/s40204-022-00181-y
P Bargavi, R Riju Chandran, D Durgalakshmi, P Rajashree, R Ramya, S Balakumar
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引用次数: 2

Abstract

A unique implant coated substrate with dual-drug-eluting system exhibiting antibacterial, anti-inflammatory, and bone regenerative capacity has been fabricated using spray pyrolysis deposition (SPD) method. Bioglass (BG) and BG-alumina (BG-Al) composites coatings with different concentrations of Al incorporated on BG network over the Cp-Ti substrate were fabricated using SPD technique. Phase purity of BG and BG-Al composites were analyzed by XRD in which Na2Ca2Si3O9 and β-Na2Ca4(PO4)2SiO4) and Na7.15(Al7.2Si8.8O32) phases were formed. Surface morphology of the coated substrates was analyzed by SEM. Uniformity of the coatings were evaluated by surface profilometer and the uniform distribution the nanoparticles were confirmed with Elemental mapping. Systematically, each apatite layer formation on coated substrate was confirmed by immersing the samples for 1, 3, and 7 days in simulated body fluid and the needle-like structure was characterized using SEM. Cumulative release of Tetracycline hydrochloride (Tet) antibiotic and Dexamethasone (Dex) anti-inflammatory drug-loaded BG-Al and BG-Al composite-coated substrate were studied for 24 h. Antibacterial activity of the coated substrates were evaluated by time-dependent growth inhibition and minimal inhibitory concentration (MIC) assays in which BG-Al and BG-Al composite loaded with Tet showed considerable growth inhibition against S. aureus. Osteoblast-like cells (MG-63) exhibited profound proliferation with no cytotoxic effects which was due to release of Dex drug-coated substrates. Thus, surface modification of Cp-Ti substrate with BG, BG-Al composites coatings loaded with Tet and Dex drug can be considered for post-operative orthopedic implant infection application.

Abstract Image

Abstract Image

药物灌注al2o3生物活性玻璃涂层在骨科感染治疗中的应用。
采用喷雾热解沉积(SPD)方法制备了一种独特的具有抗菌、抗炎和骨再生能力的双药物洗脱系统的植入物涂层基板。采用SPD技术制备了生物玻璃(BG)和BG-氧化铝(BG-Al)复合涂层。采用XRD分析了BG和BG- al复合材料的相纯度,其中形成了Na2Ca2Si3O9、β-Na2Ca4(PO4)2SiO4)和Na7.15(Al7.2Si8.8O32)相。利用扫描电镜分析了涂层基底的表面形貌。用表面轮廓仪评价了镀层的均匀性,并用元素映射法确定了纳米颗粒的均匀分布。系统地,通过将样品浸泡在模拟体液中1,3和7天来确认涂层基底上形成的每个磷灰石层,并使用SEM对针状结构进行表征。研究了盐酸四环素(Tet)抗生素和地塞米松(Dex)抗炎药物负载BG-Al和BG-Al复合包被底物的累积释放24 h。通过时间依赖性生长抑制和最小抑制浓度(MIC)测定来评估包被底物的抗菌活性,结果表明BG-Al和负载Tet的BG-Al复合包被底物对金黄色葡萄球菌具有明显的生长抑制作用。成骨细胞样细胞(MG-63)表现出深度增殖,没有细胞毒性作用,这是由于Dex药物包被底物的释放。因此,可以考虑用BG、BG- al复合涂层负载Tet和Dex药物对Cp-Ti基板进行表面修饰,用于骨科植入物术后感染的应用。
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来源期刊
Progress in Biomaterials
Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
9.60
自引率
4.10%
发文量
35
期刊介绍: Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.
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