Mashudi, S. Pudjiono, D. Setiadi, N. Hidayati, M. Sulaeman, L. Baskorowati, R. Hendrati, M. Susanto
{"title":"Growth Variation of Swietenia macrophylla King. Progeny Trial at 18 Months of Age in Trenggalek East Java","authors":"Mashudi, S. Pudjiono, D. Setiadi, N. Hidayati, M. Sulaeman, L. Baskorowati, R. Hendrati, M. Susanto","doi":"10.2991/absr.k.210621.009","DOIUrl":"https://doi.org/10.2991/absr.k.210621.009","url":null,"abstract":"Swietenia macrophylla King. as known as wide leaf mahogany is a type of exotic species from Latin America that is common planted in Indonesia. This species is widely cultivated in several regions in Indonesia due to the high demand for carpentry timber. Therefore, to meet the high demand of wide leaf mahogany wood, the development of plantation forests of S. macrophylla with high productivity is very much needed. Development of plantation forests with high productivity requires the support of improvement seeds. Therefore, this study was conducted to determine the survival rate, growth variation between land race and between mother trees of 18 months old of S. macrophylla progeny trial. This progeny trial at the end of the selection can be converted into a seedling seed orchard (SSO) to produce the improvement seeds. Incomplete Block Design with two factors, i.e. land race (5 levels) and mother tree (50 levels) was used in this study. The results of the analysis showed that the survival rates of plant was significantly different between the land race but not among the mother trees. The average of survival rate of this species is only 71.58%, due to the landslide during the rainy season, burnt during the dry season and also exposed to stem borer attacks. Growth parameters (height and stem diameter) were significantly different between land race and mother tree. It showed that the Bondowoso land race exhibited the best performance of height and stem diameter, respectively 151.55 cm and 21.97 mm. The best mother tree in terms of growth (height and stem diameter) were exhibited on 19 individual tresses, with a range from 130.38 to 171.64 cm for height character and from 20.46 to 26.47 mm for stem diameter character.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80294752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Species and Abundance of Subterranean Termites (Insecta: Isoptera) in the Area of University of Bengkulu","authors":"Helmiyetti, Darmi, Rezan Okto Wesa","doi":"10.2991/absr.k.210621.026","DOIUrl":"https://doi.org/10.2991/absr.k.210621.026","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89397484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Baskorowati, R. Hendrati, N. Hidayati, Mashudi, M. Susanto, D. Setiadi
{"title":"Low Reproduction Success of Calliandra calothyrsus and It’s Implication for Breeding","authors":"L. Baskorowati, R. Hendrati, N. Hidayati, Mashudi, M. Susanto, D. Setiadi","doi":"10.2991/absr.k.210621.008","DOIUrl":"https://doi.org/10.2991/absr.k.210621.008","url":null,"abstract":"In anticipating the rapid decrease of energy resource, especially fossil fuel, immediate actions are required to fulfil the needs of highly increased population in the world. Innovation to produce renewable energy from easily utilized raw materials should meet society’s needs including renewable raw materials from wood biomass for energy. Genetically improved Calliandra calothyrsus has been developed for this purpose. Due to low success rate of its reproduction, study on its reproductive biology was undertaken. The study was carried out during the flowering seasons of C. calothyrsus, by examining flower morphology, pollen, and pistil viabilities as wee as insect visitors to this species’ flowers. The study showed that the flowers of C. calothyrsus are borne on inflorescences (spikes) and carrying an average of 354 individual flowers, which develop acropetally. Pistils (female organ) cease to develop by the 2 to 3 day after their appearance, and the flowering is becoming ceased then. Pistils showed maturity during the period of 09.00 WIB 10.00 WIB and pollen is still viable from 1 to 3 days after the pollen opening. Three most frequent visitors (families: Apidae, Vespidae, Formicidae) and one order lepidoptera were found visiting C. calothyrsus flowers However, the reproductive success of C. calotyrsus is found to be very low, which is only 0.024. These preliminary results confirmed that the low reproductive success of C. calothyrsus is due to the structure and development of flowers allowing the incidences of both outcrossing and self-pollination. The limitation time of pistil maturity might also contribute to the low reproductive success of this species.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88726717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Debie Rizqoh, Sipriyadi, Shella Sharon, I. Rachmawati, Wulan Okta Kumala, C. N. Putri
{"title":"Exploration of Phyllosphere Bacteria From Andaliman (Zanthoxylum acanthopodium DC.)","authors":"Debie Rizqoh, Sipriyadi, Shella Sharon, I. Rachmawati, Wulan Okta Kumala, C. N. Putri","doi":"10.2991/absr.k.210621.075","DOIUrl":"https://doi.org/10.2991/absr.k.210621.075","url":null,"abstract":"Phyllosphere bacteria were bacteria that inhabit around the leaf surface. Several studies showed the potential of phyllosphere bacteria in producing bioactive compounds, one of which is antimicrobial compounds. Andaliman (Zanthoxylum acanthopodium DC.) was a plant from the Rutaceae family that was commonly found in North Sumatra, and its fruit was widely used as a spice in traditional cooking by the Batak tribe. Several studies showed that the andaliman terpenoid has antioxidant and antimicrobial activity and an immunostimulating effect. This study aimed to explore the potential of andaliman phyllosphere bacteria. The research design method was a qualitative experimental model. The research stages used in compiling this paper consisted of phyllosphere bacteria isolation, phyllosphere bacteria morphological characterization, and Gram stain. This study succeeded in isolating 206 colonies of andaliman phyllosphere bacteria with various colony forms and cell morphology. From 64 samples of phyllosphere bacterial isolate observed, we found 25 different colony forms, and all samples were Gram-positive bacteria. Microscopic observations of gram staining showed that 44 isolates (68.75%) were cocci, 17 isolates (26.56%) were in the form of bacillus, and three isolates (4.68%) were in the form of coccobacillus.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88826121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sperm Motility of Brek Fish (Systomus Orphoides) Using Soy Extract as a Supplementation Extender","authors":"Marindha Febriani, Abinawanto, R. R. Dewi","doi":"10.2991/absr.k.210621.047","DOIUrl":"https://doi.org/10.2991/absr.k.210621.047","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87806499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation","authors":"Rifqi Ramadhana, Abinawanto, R. Gustiano","doi":"10.2991/absr.k.210621.050","DOIUrl":"https://doi.org/10.2991/absr.k.210621.050","url":null,"abstract":"The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4 ̊C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 ̊C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 + 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4 ̊C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82901308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Darwis, R. H. Wibowo, Sipriyadi, Raden Roro Upiek Ngesti Wibawaning Astuti, Lam Way Sitorus
{"title":"The Influence of Green, Corn, and Rice Seed Media Composition on Mycelium Growth of F2 Main Seeds (Pleurotus ostreatus (Jacq. Ex Fr) Kummer)","authors":"W. Darwis, R. H. Wibowo, Sipriyadi, Raden Roro Upiek Ngesti Wibawaning Astuti, Lam Way Sitorus","doi":"10.2991/absr.k.210621.092","DOIUrl":"https://doi.org/10.2991/absr.k.210621.092","url":null,"abstract":"White oyster mushroom (Pleurotus ostreatus) is a type of fungus wood that has a high nutritional content. White oyster mushroom cultivation begins with the provision of good quality seeds which include 4 stages, namely F1 seeds, F2, F3 and baglog. This study aims to influence the composition of the green bean, maize and rice grain media composition on the growth of mycelium F2 parent white oyster mushroom. This research was conducted at the Microbiology Laboratory, Department of Biology. The research design used a randomized block design (RBD), with the parameters of observing the propagation length, color and thickness of the mycelium. The results showed that the composition of different media had a significant effect on the propagation of mycelium in P2, P9, P10, P18, P19, P20 with the highest length of 14 cm, compact white color and evenly thick on combination media. Environmental conditions suitable for the growth of mycelium seeds F2 white oyster mushroom include temperature 27-28 ̊C, humidity 60-62%, and light intensity 24-54 Lux.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87739766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Surianti, Fitratul Muaddama, R. S. Putri, Hasrianti, Damis, Wahyudi
{"title":"Application of Fermented Rice Bran Using Lactobacillus sp. in Artificial Feed For Survival Rate and FCR of Tilapia (Oreochromis niloticus)","authors":"Surianti, Fitratul Muaddama, R. S. Putri, Hasrianti, Damis, Wahyudi","doi":"10.2991/absr.k.210621.088","DOIUrl":"https://doi.org/10.2991/absr.k.210621.088","url":null,"abstract":"Rice bran is the result of the process of milling rice plants into rice, but rice bran has not been utilized properly in the Sidrap area. Feeding with the addition of fermented rice bran using Lactobacillus sp. is expected to increase the use of feed in tilapia aquaculture. This research aims to realize the fermentation of rice bran on the survival parameters and feed conversion ratio (fcr) of tilapia. This research used a completely randomized design with three replications and four treatments, namely (A) 10% dose of rice bran in feed, (B) 15% dose of rice bran in feed, (C) 20% dose of rice bran in feed, and (D) control. feed. The size of the tilapia used is 1.5 g / fish with a density of 20 individuals / 85 L of freshwater. The number of feeding four times a day and given a test feed of 5% of the bodyweight of the fish. The results showed that the bran flour was fermented using Lactobacillus sp. Significantly different in the survival rate and the ratio of tilapia feed conversion and water quality during the study is suitable for use in tilapia fish maintenance. The highest value resulting from the addition of fermented rice bran 15% (18.33) and 20% (19.33) for survival, while the lowest for control (16.67) and feed conversion ratio showed the best results with the provision of fermented rice bran 20 % (3.03).","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74208293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suhartina, Purwantoro, N. Nugrahaeni, M. J. Mejaya
{"title":"Response of Soybean Lines to Drought Stress During Reproductive Phase","authors":"Suhartina, Purwantoro, N. Nugrahaeni, M. J. Mejaya","doi":"10.2991/absr.k.210621.055","DOIUrl":"https://doi.org/10.2991/absr.k.210621.055","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73680420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Novianti, Tazkia Ayu Safitri, F. D. Wahyuni, I. Jamilah, S. Ilyas
{"title":"The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice (Mus musculus)","authors":"T. Novianti, Tazkia Ayu Safitri, F. D. Wahyuni, I. Jamilah, S. Ilyas","doi":"10.2991/absr.k.210621.071","DOIUrl":"https://doi.org/10.2991/absr.k.210621.071","url":null,"abstract":"Some genes play a role in the tissue regeneration process and they have specific functions. One of them is the platelet-derived growth factors (PDGF) gene that have a role in the wound healing process because it induces cell mitosis and thereby causes cell proliferation. PDGF protein expression is thought to chemotactically recruit many cell types including smooth muscle, fibroblasts, and monocytes to migrate to the wound site. These research aims are to analyze the role of PDGF gene expression in the process of digit tip mice tissue regeneration. This study used 24 mice (Mus musculus) were amputated at the digit tip and the growth was observed on days 0, 1, 3, 5, 10, 15, and 25. We isolated RNA from each tissue after amputation on days 0, 1, 3, 5, 10, 15, and 25 and analyzed the RNA expression of the PDGF gene using the qPCR method. PDGF gene expression reached its peak on day 10 on the granule phase and decreased until day 25. The peak expression of the PDGF gene indicates the role of the gene in the tissue regeneration of digit tip mice. The normality test for PDGF gene expression was normally distributed (p> 0, 05) and the variation test between each treatment was different significantly (p <0.05).","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79794667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}