Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)最新文献_第5页

Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation 脱脂乳对短期保存鲈鱼(systemus orphoides, valcienes, 1842)精子活力的影响

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.050

Rifqi Ramadhana, Abinawanto, R. Gustiano

{"title":"Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation","authors":"Rifqi Ramadhana, Abinawanto, R. Gustiano","doi":"10.2991/absr.k.210621.050","DOIUrl":"https://doi.org/10.2991/absr.k.210621.050","url":null,"abstract":"The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4 ̊C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 ̊C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 + 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4 ̊C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82901308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Difference in F2 Isoprotane Levels in Male Model Mice (Mus musculus L.) in Maximum Physical Exercise Without Supplementation 最大运动条件下雄性模型小鼠(小家鼠)F2异丙烷水平的差异

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.042

E. Yuniarti, M. Des, P. M. Indika

引用次数: 0

Exploration of Phyllosphere Bacteria From Andaliman (Zanthoxylum acanthopodium DC.) 安达曼岛层球细菌的探索(Zanthoxylum acanthopodium DC.)

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.075

Debie Rizqoh, Sipriyadi, Shella Sharon, I. Rachmawati, Wulan Okta Kumala, C. N. Putri

{"title":"Exploration of Phyllosphere Bacteria From Andaliman (Zanthoxylum acanthopodium DC.)","authors":"Debie Rizqoh, Sipriyadi, Shella Sharon, I. Rachmawati, Wulan Okta Kumala, C. N. Putri","doi":"10.2991/absr.k.210621.075","DOIUrl":"https://doi.org/10.2991/absr.k.210621.075","url":null,"abstract":"Phyllosphere bacteria were bacteria that inhabit around the leaf surface. Several studies showed the potential of phyllosphere bacteria in producing bioactive compounds, one of which is antimicrobial compounds. Andaliman (Zanthoxylum acanthopodium DC.) was a plant from the Rutaceae family that was commonly found in North Sumatra, and its fruit was widely used as a spice in traditional cooking by the Batak tribe. Several studies showed that the andaliman terpenoid has antioxidant and antimicrobial activity and an immunostimulating effect. This study aimed to explore the potential of andaliman phyllosphere bacteria. The research design method was a qualitative experimental model. The research stages used in compiling this paper consisted of phyllosphere bacteria isolation, phyllosphere bacteria morphological characterization, and Gram stain. This study succeeded in isolating 206 colonies of andaliman phyllosphere bacteria with various colony forms and cell morphology. From 64 samples of phyllosphere bacterial isolate observed, we found 25 different colony forms, and all samples were Gram-positive bacteria. Microscopic observations of gram staining showed that 44 isolates (68.75%) were cocci, 17 isolates (26.56%) were in the form of bacillus, and three isolates (4.68%) were in the form of coccobacillus.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88826121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Influence of Green, Corn, and Rice Seed Media Composition on Mycelium Growth of F2 Main Seeds (Pleurotus ostreatus (Jacq. Ex Fr) Kummer) 绿色、玉米和水稻种子培养基组成对F2主种子(平菇)菌丝生长的影响。前主任(Kummer)

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.092

W. Darwis, R. H. Wibowo, Sipriyadi, Raden Roro Upiek Ngesti Wibawaning Astuti, Lam Way Sitorus

{"title":"The Influence of Green, Corn, and Rice Seed Media Composition on Mycelium Growth of F2 Main Seeds (Pleurotus ostreatus (Jacq. Ex Fr) Kummer)","authors":"W. Darwis, R. H. Wibowo, Sipriyadi, Raden Roro Upiek Ngesti Wibawaning Astuti, Lam Way Sitorus","doi":"10.2991/absr.k.210621.092","DOIUrl":"https://doi.org/10.2991/absr.k.210621.092","url":null,"abstract":"White oyster mushroom (Pleurotus ostreatus) is a type of fungus wood that has a high nutritional content. White oyster mushroom cultivation begins with the provision of good quality seeds which include 4 stages, namely F1 seeds, F2, F3 and baglog. This study aims to influence the composition of the green bean, maize and rice grain media composition on the growth of mycelium F2 parent white oyster mushroom. This research was conducted at the Microbiology Laboratory, Department of Biology. The research design used a randomized block design (RBD), with the parameters of observing the propagation length, color and thickness of the mycelium. The results showed that the composition of different media had a significant effect on the propagation of mycelium in P2, P9, P10, P18, P19, P20 with the highest length of 14 cm, compact white color and evenly thick on combination media. Environmental conditions suitable for the growth of mycelium seeds F2 white oyster mushroom include temperature 27-28 ̊C, humidity 60-62%, and light intensity 24-54 Lux.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87739766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm Motility of Brek Fish (Systomus Orphoides) Using Soy Extract as a Supplementation Extender 大豆提取物对裂口鱼精子活力的影响

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.047

Marindha Febriani, Abinawanto, R. R. Dewi

引用次数: 0

The Species and Abundance of Subterranean Termites (Insecta: Isoptera) in the Area of University of Bengkulu 白蚁(昆虫目:等翅目)在蚌库鲁大学地区的种类和数量

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.026

Helmiyetti, Darmi, Rezan Okto Wesa

引用次数: 0

Low Reproduction Success of Calliandra calothyrsus and It’s Implication for Breeding calothyrsus的低繁殖成功率及其育种意义

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.008

L. Baskorowati, R. Hendrati, N. Hidayati, Mashudi, M. Susanto, D. Setiadi

{"title":"Low Reproduction Success of Calliandra calothyrsus and It’s Implication for Breeding","authors":"L. Baskorowati, R. Hendrati, N. Hidayati, Mashudi, M. Susanto, D. Setiadi","doi":"10.2991/absr.k.210621.008","DOIUrl":"https://doi.org/10.2991/absr.k.210621.008","url":null,"abstract":"In anticipating the rapid decrease of energy resource, especially fossil fuel, immediate actions are required to fulfil the needs of highly increased population in the world. Innovation to produce renewable energy from easily utilized raw materials should meet society’s needs including renewable raw materials from wood biomass for energy. Genetically improved Calliandra calothyrsus has been developed for this purpose. Due to low success rate of its reproduction, study on its reproductive biology was undertaken. The study was carried out during the flowering seasons of C. calothyrsus, by examining flower morphology, pollen, and pistil viabilities as wee as insect visitors to this species’ flowers. The study showed that the flowers of C. calothyrsus are borne on inflorescences (spikes) and carrying an average of 354 individual flowers, which develop acropetally. Pistils (female organ) cease to develop by the 2 to 3 day after their appearance, and the flowering is becoming ceased then. Pistils showed maturity during the period of 09.00 WIB 10.00 WIB and pollen is still viable from 1 to 3 days after the pollen opening. Three most frequent visitors (families: Apidae, Vespidae, Formicidae) and one order lepidoptera were found visiting C. calothyrsus flowers However, the reproductive success of C. calotyrsus is found to be very low, which is only 0.024. These preliminary results confirmed that the low reproductive success of C. calothyrsus is due to the structure and development of flowers allowing the incidences of both outcrossing and self-pollination. The limitation time of pistil maturity might also contribute to the low reproductive success of this species.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88726717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of Soybean Lines to Drought Stress During Reproductive Phase 大豆系生殖期对干旱胁迫的响应

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.055

Suhartina, Purwantoro, N. Nugrahaeni, M. J. Mejaya

引用次数: 0

The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice (Mus musculus) PDGF基因在指尖小鼠组织再生中的作用

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.071

T. Novianti, Tazkia Ayu Safitri, F. D. Wahyuni, I. Jamilah, S. Ilyas

{"title":"The Role of PDGF Gene in Tissue Regeneration of Digit Tip Mice (Mus musculus)","authors":"T. Novianti, Tazkia Ayu Safitri, F. D. Wahyuni, I. Jamilah, S. Ilyas","doi":"10.2991/absr.k.210621.071","DOIUrl":"https://doi.org/10.2991/absr.k.210621.071","url":null,"abstract":"Some genes play a role in the tissue regeneration process and they have specific functions. One of them is the platelet-derived growth factors (PDGF) gene that have a role in the wound healing process because it induces cell mitosis and thereby causes cell proliferation. PDGF protein expression is thought to chemotactically recruit many cell types including smooth muscle, fibroblasts, and monocytes to migrate to the wound site. These research aims are to analyze the role of PDGF gene expression in the process of digit tip mice tissue regeneration. This study used 24 mice (Mus musculus) were amputated at the digit tip and the growth was observed on days 0, 1, 3, 5, 10, 15, and 25. We isolated RNA from each tissue after amputation on days 0, 1, 3, 5, 10, 15, and 25 and analyzed the RNA expression of the PDGF gene using the qPCR method. PDGF gene expression reached its peak on day 10 on the granule phase and decreased until day 25. The peak expression of the PDGF gene indicates the role of the gene in the tissue regeneration of digit tip mice. The normality test for PDGF gene expression was normally distributed (p> 0, 05) and the variation test between each treatment was different significantly (p <0.05).","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79794667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Seed Source Variation on Syzygium cumini (L.) Skeels. Seedlings Growth in Ciamis District, West Java Province 种子源变异对小茴香的影响斯基尔。西爪哇省Ciamis区的幼苗生长情况

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI: 10.2991/absr.k.210621.056

A. Sudomo, L. Pieter

{"title":"Effect of Seed Source Variation on Syzygium cumini (L.) Skeels. Seedlings Growth in Ciamis District, West Java Province","authors":"A. Sudomo, L. Pieter","doi":"10.2991/absr.k.210621.056","DOIUrl":"https://doi.org/10.2991/absr.k.210621.056","url":null,"abstract":"Syzygium cumini included in the family Myrtaceae is one type of plant that is hard to find. The benefits of S. cumini plants include seeds as a cure for diabetes, fruit as an antioxidant and anticancer, fruit skin as a medicine for dysentery and leaves as a reinforcement of teeth and gums. This research aimed to study the effect of seed population sources on the growth of S. cumini seedling. Seed exploration was carried out in 3 different S. cumini habitats, (1) Gunungkidul District (2) Yogyakarta District and (3) Cikampek District. CRD (Complete Random Design) was used with each population consisting of three replications (50 seeds/replication). The seed source showed significantly different results on the growth of S. cumini seedlings (height, diameter, number of leaves, wet weight of stem and leaves and dry weight of stem and leaves). However, the variation of seed sources did not significantly differ on survival, root wet mean, root dry weight, top-root ratio and seed quality index. Height, diameter, number of leaves, comparison starting from the greatest were Gunungkidul (30.42 cm / 2.93 mm / 21.42), Cikampek (24.64 cm / 2.82 mm / 21.06) and Yogyakarta (21.27 cm / 2.45 mm / 19.27). While The stem and leaves wet weight and stem and leaf biomass comparison from the highest were Gunungkidul (6.84 gram, 2.10 gram), Yogyakarta (4.95 gram / 1.59 gram) and Cikampek (4.24 gram / 1.47 gram). Moreover, seeds from the Gunungkidul population produced better growth than other populations. This study's recommendation is to develop rare plants of S. cumini species that will have the best growth by using seeds from the Gunungkidul","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87529712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0