Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation

Abstract

The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4 ̊C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 ̊C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 + 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4 ̊C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.