Effect of Skim Milk on Sperm Motility of Brek Fish, Systomus orphoides (Valenciennes, 1842) for Short Term Preservation

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) Pub Date : 2021-06-23 DOI:10.2991/absr.k.210621.050

Rifqi Ramadhana, Abinawanto, R. Gustiano

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Abstract

The purpose of this research was to evaluate the effect of skim milk on sperm motility of Brek fish, Systomus orphoides (Valenciennes, 1842) after 48 hours in 4 ̊C. The method in this research is sperm was collected by hand stripping and was diluted by diluent, which consisted of the fish ringer, skim milk and methanol 10%. The ratio of sperm to diluent is 1:9. The concentrations of skim milk used in this research were 0%, 5%, 10%, 15%, and 20%, respectively. The sperm was stored at 4 ̊C for 48 hours after diluted sperm is thawed in room temperate for 1 minute and sperm motility was observed in improved Neubauer with a light microscope.The motility percentage was analyzed using ANOVA and Tukey test. According to the Annova test (P>0.05), 10% of skim milk showed the highest sperm motility (92.18 + 3.05) % after five replication per dose. Ten percent of skim milk was the optimum concentration to preserve sperm motility at 4 ̊C for 48 hours compared to 0%, 5%, 15%, and 20%. The result of each concentration is 90,85%, 84,68%, 79,54%, and 74,14% respectively.

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脱脂乳对短期保存鲈鱼(systemus orphoides, valcienes, 1842)精子活力的影响

本研究的目的是评价脱脂乳对Brek fish, systemus orphoides (valencienne, 1842)在4℃条件下48小时精子活力的影响。本研究的方法是用手剥取精子，用稀释剂稀释，稀释剂由鱼环、脱脂牛奶和10%的甲醇组成。精子和稀释剂的比例是1:9。本研究中使用的脱脂牛奶浓度分别为0%、5%、10%、15%和20%。将稀释后的精子室温解冻1分钟后，于4℃保存48 h，光镜下观察改良Neubauer精子活力。运动率分析采用方差分析和Tukey检验。经anova检验(P>0.05)， 10%脱脂乳每剂量重复5次后精子活力最高(92.18 + 3.05)%。与0%、5%、15%和20%的脱脂牛奶相比，10%的脱脂牛奶是在4℃条件下保持精子活力48小时的最佳浓度。各浓度测定结果分别为90、85%、84、68%、79、54%和74、14%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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