{"title":"The Effectiveness of Piper betel L. Leaf Extract as a Natural Electric Mosquito","authors":"S. Safrida, F. A. Ulhusna","doi":"10.2991/absr.k.210621.051","DOIUrl":"https://doi.org/10.2991/absr.k.210621.051","url":null,"abstract":"Mosquitoes are known as insects that interfere with comfort. Mosquitoes are also known as vectors or transmitters of several types of dangerous and deadly diseases for humans, one of which is dengue fever. Therefore, an alternative is needed to reduce this species. The alternative that can be done is by providing vegetable insecticides. Betel leaf has the potential to be used as a base for making plant-based insecticides that are environmentally friendly because they contain chemical compounds in the form of phenols and derivatives such as chavikol and eugenol, alkaloids, tannins, flavonoids, saponins and essential oils. This study aims to determine the concentration of betel leaf extract (Piper betel L.) which is effective for killing mosquitoes. This study used an experimental method using a completely randomized design (CRD) with 6 treatments and 4 replications, namely KN as negative control group, KP as control positive (100% HIT brand liquid), P1 25% extract, P2 50% extract, P3 75% extract, and P4 100% extract. The number of dead mosquitoes (mortality) were observed after 1 hour of treatment. Data were analyzed using variance analysis (ANAVA). The results of this study were the administration of various concentrations of betel leaf extract (Piper betel L.) significantly (p <0.05) to kill mosquitoes. The greatest frequency of mosquito mortality was found at the P4, the highest extract concentration. The conclusion of this study is that betel leaf extract for 1 hour had a significant effect on mosquito mortality.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73383020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Sartika, A. Irsyad, Rizal Hermawan S. Budi, Wahyu Dwi Soemantri, B. Daryono
{"title":"The Phenotype Characters Stability of Hybrid Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) ♀ ‘Maduri’ and ♂ ‘Putri Delima’","authors":"D. Sartika, A. Irsyad, Rizal Hermawan S. Budi, Wahyu Dwi Soemantri, B. Daryono","doi":"10.2991/absr.k.210621.004","DOIUrl":"https://doi.org/10.2991/absr.k.210621.004","url":null,"abstract":"Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is one of the horticultural crops members of Cucurbitaceae family, which is known to have high economic value and public interest. Since the entry into Indonesia, the application of watermelon plant breeding has grown to produce new superior varieties, hence not only able to meet market demand, but also to have implications for the progress of the agribusiness sector and national food security. Watermelon ♀ ‘Maduri’ with ♂ ‘Putri Delima’ cultivar hybrid produce an oblong-shaped fruit, has a dark green skin tone with dark green streaks, orange flesh fruit, and sweet taste. However, to be certified and become a cultivar that can be cultivated extensively, the new varieties need to have an adaptive and stable character. This study aims to determine the phenotype character and stability of watermelon ♀ ‘Maduri’ with ♂ ‘Putri Delima’ cultivar hybrid which is cultivated in Jamusan Village, Prambanan Sub-district, Sleman, D.I.Yogyakarta fields. Furthermore, 1 randomly collected watermelon samples were taken. Then, the qualitative and quantitative characters of samples were observed. The quantitative data were analyzed using Microsoft Excel 2016 with ANOVA single factor test at 1% and 5% level. The result of ANOVA test at 5% significance level shows F value at 0,00055 and Fcrit value at 4,259677273, while at the l% significance level, F value obtained at 0,00055 and the Fcrit value obtained at 7,822871. F value is smaller than Fcrit value at 5% and 1% significance level so there is no significance difference. Thus, the phenotype character of F2 watermelon is deemed stable.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84766047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Elicitor Effect of Chitosan on In Vitro Culture of Different Explants of Physalis Accessions From East Java","authors":"R. Mastuti, J. Batoro, Budi Waluyo","doi":"10.2991/absr.k.210621.064","DOIUrl":"https://doi.org/10.2991/absr.k.210621.064","url":null,"abstract":"Chitosan is a natural molecule derived from arthropods and fungi that can stimulate many physiological responses in plants. Chitosan had also shown positive effect on in vitro shoot regeneration. Therefore, this research aims to observe the response of shoot growth of in vitro Physalis plant accessions obtained from several areas in Madura Island and East Java on chitosan effect. Two types of explants shoot tips and in vitro nodal explants for each Physalis plant accession were cultured on elicitation medium (MS basal medium supplemented with BAP 2 mg / l and IAA 0.05 mg /l) containing chitosan elicitor (0, 75, 125 mg /l). The number and height of shoots were observed weekly for six weeks. The results showed that both types of explants from all accessions were able to regenerate and multiply shoots however shoot tip explants showed better growth. A2 accessions from Madura Sampang was able to regenerate shoots in high a number than other accessions on chitosan concentration of 125 mg / l. These results provide a future prospect to study synthesis of secondary metabolites in Physalis accession induced by chitosan elicitors.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89702914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study on Maintenance and Utilization of Tamed Sumatran Elephants (Elephas maximus sumatranus Temminck 1847) at the Elephant Training Center in the Seblat TWA Area, Bengkulu","authors":"Rizwar, Darmi, C. H. Sukoco","doi":"10.2991/absr.k.210621.031","DOIUrl":"https://doi.org/10.2991/absr.k.210621.031","url":null,"abstract":"In the range of 23 years, from 1992 to 2015, there has been a drastic decrease in the number of individual tamed Sumatran elephants (Elephas maximus sumatranus Temminck 1847) at the Seblat Elephant Training Center (PLG) from 40 individuals to 18 individuals. Decreasing the size of the tamed elephant population is suspected to be inappropriate elephant maintenance at PLG, disease, old age and malnutrition. Good governance in the maintenance and utilization of tamed elephants at PLG is thought to be able to increase the life span of elephants and their reproductive capacity. This study aims to examine the management of PLG, especially in the maintenance and utization of tamed elephants conducted by BKSDA, Bengkulu Province. The study was conducted by direct survey method to PLG Seblat and interviewing as well as searching various relevant literatures. The parameters observed refer to the minimum standard reference for handling elephants in captivity. The results of the study found that the maintenance and utilization of the tamed elephants at PLG were only partially fulfilling the minimum standard of management of the guided elephants such as training, grazing, trekking and patrolling. While the form of maintenance that is still below the standard that needs to be improved is related to the handling of elephant health and mitigation of tamed elephant conflict with communities around","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78402838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Santika Lusia Utami, Yogi Adhi Nugroho, Luthfiana Hardianingtyas, F. Kamilah
{"title":"In-Silico Studies of Antitumor Activity From Red Ginger (Alpinia purpurata Vieill) Bioactive Compounds","authors":"Santika Lusia Utami, Yogi Adhi Nugroho, Luthfiana Hardianingtyas, F. Kamilah","doi":"10.2991/absr.k.210621.067","DOIUrl":"https://doi.org/10.2991/absr.k.210621.067","url":null,"abstract":"Many plants from the genus Alpinia are known to have several bioactive compounds that can act as an antitumor in cancer treatments. However, the potency of the bioactive compounds from red ginger as an antitumor agent has not been reported before. This research aims to study the inhibitory activity of several red ginger bioactive compounds against antitumor AKT1 protein through in-silico analysis. In this study, four identified bioactive compounds namely rutin, sitosteryl 3-O-6-palmytoil-β-d-glucoside, kumatakenin, kaempferol-3-o-glucuronide were used as a ligand for molecular interactions. The 3D structure of targetted protein AKT1 (PDB ID: 3O96) was obtained from the PDB database. [6]-shogaol, an antiproliferative and AKT1 inhibitor, was used as a control. In-silico docking analysis is performed in PyRx with the AutoDock Vina program. All bioactive compounds used in this experiment demonstrated good antitumor activity by binding to the inhibitor site of AKT1 protein. Rutin displayed the best potency as an inhibitor to AKT1 with the optimum binding energy of 11,3 kcal/mol as compared to control with -7,4 kcal/mol. These results suggest that bioactive compounds from red ginger may have the potency as an antitumor and can be developed to treat cancer.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"404 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74348376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic Variation of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) Based on Mitochondrial DNA Cytochrome B Gene","authors":"Deby Permana, Jarulis, Sipriyadi, Santi Nurul Kamilah","doi":"10.2991/absr.k.210621.058","DOIUrl":"https://doi.org/10.2991/absr.k.210621.058","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86814015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection Contaminant DNA of a Pig on a Sample Meat Mills in Some City Markets Bengkulu, Using a Genetic Marker DNA Mitochondria Cytochrome B","authors":"C. Muslim, Iin Isnaini, Sipriyadi","doi":"10.2991/absr.k.210621.057","DOIUrl":"https://doi.org/10.2991/absr.k.210621.057","url":null,"abstract":"The purpose of the study was to find out if there was a mixture of pork in ground beef samples in some Bengkulu city markets using the molecular Genetic DNA mitochondrial cytochrome b. The research was conducted from December 2019 to May 2020. Sampling was conducted at 10 meat mills in Bengkulu City (Pasar Panorama, Pasar Minggu, Pasar Barukoto, and Sungai Serut Fish Market). DNA isolation, DNA amplification, and electrophoresis are carried out in the biotechnology laboratory, Department of Biology, University of Bengkulu. The meat samples that had been milled were then performed genome DNA isolation following the Qiagen DNeasy for Food Kit protocol. PCR (Polymerous Chain Reaction) is carried out as many as 30 cycles with Primer Forward referring to and primary Reverse Mix (pork, cow, and chicken), Reverse Pork for pigs and Reverse cow for cows. PCR products are electrophobic on 1% agarose gel and then visualized under UV light using Gel Document System Axygen. As a result of the study, on the primary use of Rcow all samples contained cow DNA. In primary use reverse pork, no DNA tape is amplified. In the primary use of Reverse mix from ten samples all containing cow DNA, one sample is thought to contain pig DNA i.e. s7 samples, and six of them contain chicken DNA (S1, S4, S5, S6, S7, and S8). Generally, meat milling in Bengkulu city located in Pasar Panorama, Pasar Minggu, Pasar Barukoto, and Sungai Serut Fish Market is not tainted with pork even though one sample is found, this is because it is influenced by the amount of meat that is milled still too much.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87389535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Ishartati, D. Roeswitawati, Sukardi, S. Rohman, Sudiadi
{"title":"α-Glucosidase and α -Amylase Inhibitory Activities of Jambolan (Syzygium cumini (L.) SKEELS) Fruit and Seed","authors":"E. Ishartati, D. Roeswitawati, Sukardi, S. Rohman, Sudiadi","doi":"10.2991/absr.k.210621.043","DOIUrl":"https://doi.org/10.2991/absr.k.210621.043","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80115983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Variation of Betulinic Acid Content of Andalas Tree (Morus macroura var. Macroura) in Different Localities of West Sumatra","authors":"N. Aseny, Erma Nur Atika, Syamsuardi, F. Ismed","doi":"10.2991/absr.k.210621.048","DOIUrl":"https://doi.org/10.2991/absr.k.210621.048","url":null,"abstract":"Recently, herbal ingredients have been utilized as candidate supplements or drugs to increase the resistance of body against diseases caused by viruses. In general, research is focused on plants used as herbal medicine in Indonesia. Sumatran mulberry (Morus macroura var. Macroura) is the native plant tree species in West Sumatra with the local name of the Andalas tree that has the potential to be used as a medicinal plant against the viruses desease due to its betulinic acid content. The content of secondary metabolites in a plant species can vary due to the influence of various environmental factors in their habitats. This study explored the content of betulinic acid of Morus macroura var. Macroura from four different locations in West Sumatra (Tanjung Bungo, Nagari Koto Hilalang, Paninjauan and Andaleh). Sample extracted from leaves and the level of content were examined using the Densitometric Thin-layer Chromatography (TLC) methods with the pure betulinic acid commpound as a standar. In this paper, we describe the various content of betulinic acid of Andalas Tree and discuss its potentiality as source of medicinal product based on the recent references.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79926430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Seed Micromorphology of Orthosiphon spp. and Its Relatives (Lamiaceae)","authors":"Sudarmono","doi":"10.2991/absr.k.210621.036","DOIUrl":"https://doi.org/10.2991/absr.k.210621.036","url":null,"abstract":"The genus Orthosiphon in the Lamiaceae or Mint family is closely related to Ocimum and is economically important for medicinal and ornamental plants. Even though the leaf and flower morphology look different, but the characteristics of the seeds (nutlets) are almost the same. This study aims to determine the micromorphological character of the Orthosiphon seeds using a Scanning Electron Microscope (SEM). Observation of seeds apart from using SEM also with light microscopy on four taxa of Orthosiphon spp (Orthosiphon aristatus with pink flower, O. aristatus with white flower, O. endanghidayatae sp. nov., O. radjaampatensis sp. nov. and two taxa of Ocimum (O. canum and O. basilicum) as comparisons of their close relatives in the Lamiaceae. The largest seed size in O. aristatus with purple flowers (2727 μm x 1364 μm) was elliptic oblong and the smallest size was O. hidayatae (2182 μm x 1591 μm in the oblong shape), while the color of the seeds was from blackish brown to black. A detailed explanation of the surface of the seeds, whether or not trichomes, the characters at the end of the seed axis are described in this paper. The evolution of the main seed characters in Orthosiphon is also discussed.","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91299039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}