Elicitor Effect of Chitosan on In Vitro Culture of Different Explants of Physalis Accessions From East Java

Abstract

Chitosan is a natural molecule derived from arthropods and fungi that can stimulate many physiological responses in plants. Chitosan had also shown positive effect on in vitro shoot regeneration. Therefore, this research aims to observe the response of shoot growth of in vitro Physalis plant accessions obtained from several areas in Madura Island and East Java on chitosan effect. Two types of explants shoot tips and in vitro nodal explants for each Physalis plant accession were cultured on elicitation medium (MS basal medium supplemented with BAP 2 mg / l and IAA 0.05 mg /l) containing chitosan elicitor (0, 75, 125 mg /l). The number and height of shoots were observed weekly for six weeks. The results showed that both types of explants from all accessions were able to regenerate and multiply shoots however shoot tip explants showed better growth. A2 accessions from Madura Sampang was able to regenerate shoots in high a number than other accessions on chitosan concentration of 125 mg / l. These results provide a future prospect to study synthesis of secondary metabolites in Physalis accession induced by chitosan elicitors.