生物化学与生物物理进展最新文献_第3页

Aging and related diseases: Research progress 衰老及其相关疾病:研究进展

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2014-01-01 DOI: 10.3724/SP.J.1206.2014.00036

Jun-Ping Liu

引用次数: 1

Cloning and Functional Characterization of a Novel Long Non-coding RNA Gene Associated With Hepatocellular Carcinoma 一种与肝细胞癌相关的新型长链非编码RNA基因的克隆和功能表征

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2014-01-01 DOI: 10.3724/SP.J.1206.2012.00613

Gong Zj, Ming Zhou, Peng Sp, J. Ma, Xiaoyan Li, Hongbin Huang, Hongbin Huang, Y. Li, Xiaoling Li, H. Bo, Zeng Zy, Strong, Gui-yuan Li, Xiang Jj, W. Xiong, F. Wei, Zhang Wl, Liao Qj, K. Tang, Song Yl

{"title":"Cloning and Functional Characterization of a Novel Long Non-coding RNA Gene Associated With Hepatocellular Carcinoma","authors":"Gong Zj, Ming Zhou, Peng Sp, J. Ma, Xiaoyan Li, Hongbin Huang, Hongbin Huang, Y. Li, Xiaoling Li, H. Bo, Zeng Zy, Strong, Gui-yuan Li, Xiang Jj, W. Xiong, F. Wei, Zhang Wl, Liao Qj, K. Tang, Song Yl","doi":"10.3724/SP.J.1206.2012.00613","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2012.00613","url":null,"abstract":"Recently, we sequenced the transcriptomes of a hepatocellular carcinoma biopsy and a normal liver tissue using the RNA-Sequencing(RNA-Seq) strategy based on the Next Generation Sequencing(NGS) technique, and identified several adjacent high RNA-Seq signal peaks on chromosome 11q13.1 in the hepatocellular carcinoma biopsy, while not in the normal control tissue. In this chromosome region, there is no characterized genes have been identified, implying that these RNA-Seq peaks may represent one or more novel genes. Further study was confirmed that these RNA-Seq peaks were transcribed by one novel gene. Through cloning the full length of this novel gene, we found that this novel gene transcribed many splicing isoforms, and the longest isoform is 3 562 bp. Then we deposited twelve representative RNA isoforms into the GenBank database of the National Center for Biotechnology Information(NCBI), and created the GenBank IDs from KC136297 to KC136308 for these isoforms. None significant open reading fragment(ORF) was found in any transcripts of this novel gene, implying that this gene may encodes long non-coding RNAs(lncRNAs). To further elucidate the potential transcriptional regulation mechanism of this lncRNA gene, we predicted the promoter from the upstream sequence of the lncRNA gene using bioinformatic tools, and found that there is one potential promoter in-719 to-469 bp from the transcript start site of the lncRNA gene, and there are seven Sp1, one STAT5 and one EGR1 transcription factor binding sites in the promoter region. The molecular mechanisms of the lncRNA gene in carcinogenesis and progression of hepatocellular carcinoma are worthful for further investigation.","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"85 1","pages":"153-162"},"PeriodicalIF":0.3,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78171140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

The effect of formaldehyde on cell cycle is in a concentration-dependent manner 甲醛对细胞周期的影响呈浓度依赖性

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2013-07-01 DOI: 10.3724/SP.J.1206.2013.00079

Junye Miao, Jing Lu, Ziang Zhang, Zhiqian Tong, R. He

{"title":"The effect of formaldehyde on cell cycle is in a concentration-dependent manner","authors":"Junye Miao, Jing Lu, Ziang Zhang, Zhiqian Tong, R. He","doi":"10.3724/SP.J.1206.2013.00079","DOIUrl":"https://doi.org/10.3724/SP.J.1206.2013.00079","url":null,"abstract":"A certain concentration of formaldehyde can cause protein misfolding, cell death and biological dysfunction. Though it has been reported that formaldehyde has cytotoxicity, how formaldehyde affects cell cycle of neural cells and the molecular mechanism still needs to be clarified. In this study, neuroblastoma cell line SH-SY5Y was utilized to incubate with formaldehyde and the effect of formaldehyde on cell cycle was in formaldehyde concentration-dependent manner. No significant changes in cell cycle could be detected when[FA] ≤0.1 mmol/L (cells were incubated for 48 h), while the percentages of cells in S phase and G2/M phase were markedly increased with the elevation of formaldehyde concentration (0.1 mmol/L < [FA] ≤ 0.2 mmol/L). In the medium with 0.3 mmol/L formaldehyde, 46.28% of cells were in S phase while only 16.05% of them were in G2/M phase, that is, cell proliferation was obviously inhibited under the conditions. When cells were synchronized at G2/M phase, formaldehyde (0.1∼0.3 mmol/L) could markedly increase the number of cells in S phase, though, to some extent, the number of cells in G2/M phase decreased. When cells were synchronized at S phase, 0.1 mmol/L formaldehyde could decrease the number of cells in G2/M phase, while 0.3 mmol/L formaldehyde could markedly decrease the number of cells in G2/M phase and significantly increase that in S phase. In the presence of formaldehyde, primary neurons of SD rat exhibited similar changes in cell cycle as that in SH-SY5 Y cells. Furthermore, early and late apoptosis was markedly observed when 0.1 mmol/L ≤ [FA] ≤0.2 mmol/L, while DNA were obviously damaged and most cells were apoptosis and some of them underwent necrosis when [FA] ≤ 0.3 mmol/L. In sum, formaldehyde at a low concentration (0.1 mmol/L≤ [FA] ≤0.2 mmol/L) mainly suppresses DNA synthesis in S phase via hypermethylation of global DNA, while formaldehyde at a higher concentration ([FA] ≥ 0.3 mmol/L) causes DNA damage, both of them lead to the aberrant effects on cell cycle.","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":"439 1","pages":"641-651"},"PeriodicalIF":0.3,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82881623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Inhibition of ERK1/2 Kinase Enhances BMP9-induced Osteogenic Differentiation of Mesenchymal Stem Cells*: Inhibition of ERK1/2 Kinase Enhances BMP9-induced Osteogenic Differentiation of Mesenchymal Stem Cells* 抑制ERK1/2激酶增强bmp9诱导的间充质干细胞成骨分化*:抑制ERK1/2激酶增强bmp9诱导的间充质干细胞成骨分化*

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2013-01-08 DOI: 10.3724/SP.J.1206.2012.00170

Tao Song, Juan-wen He, Jin Wang, M. Tang, Jinyong Luo

引用次数: 0

New Research Advances in Genetics Associated With High-density Lipoprotein Cholesterol*: New Research Advances in Genetics Associated With High-density Lipoprotein Cholesterol* 高密度脂蛋白胆固醇相关基因的新研究进展*:高密度脂蛋白胆固醇相关基因的新研究进展*

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2013-01-08 DOI: 10.3724/SP.J.1206.2012.00140

Xiao-Yan Liu, Qian Lu, Wu-jun Chen, Chao-ke Tang

引用次数: 0

Effects of GABA Signal on Mouse Placenta Establishment in Early-Middle Phase*: Effects of GABA Signal on Mouse Placenta Establishment in Early-Middle Phase* GABA信号对小鼠早中期胎盘形成的影响*:GABA信号对小鼠早中期胎盘形成的影响*