IF 0.2 4区生物学

生物化学与生物物理进展 Pub Date : 2023-05-16 DOI: 10.16476/j.pibb.2023.0021

唐春花 | 江寒冰 | 杨洁 | 卢晓玲 | 陈美仑 | 魏铮 | 刘一杰 | 余鹏

引用次数: 0

趋化因子CCL11-糖胺聚糖-趋化因子受体CCR3的相互作用研究趋化因子CCL11-糖胺聚糖-趋化因子受体CCR3的相互作用研究

IF 0.2 4区生物学

生物化学与生物物理进展 Pub Date : 2023-01-30 DOI: 10.16476/j.pibb.2022.0312

李冠霖 | 刘恒姮 | 丁彦之 | 李计强 | 葛保胜

引用次数: 0

醛酮还原酶AKR1Cs的表达纯化及其催化还原福美司坦的作用研究醛酮还原酶AKR1Cs的表达纯化及其催化还原福美司坦的作用研究

IF 0.2 4区生物学

生物化学与生物物理进展 Pub Date : 2019-11-14 DOI: 10.16476/j.pibb.2019.0204

孔熙 | 陶思雯 | Frank Heinrich WIELAND | 杨佑喆 | Alexander Tobias TEICHMANN | 马根 | 陈木兰 | 解河崧 | 王海 | 汪贵林 | 万闰兰

引用次数: 0

Expression analysis of ribosome-inactivating proteins from cucumber 黄瓜核糖体失活蛋白的表达分析

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2017-01-01 DOI: 10.16476/J.PIBB.2017.0311

Liuyi Dang, P. Rougé, E. Damme

{"title":"Expression analysis of ribosome-inactivating proteins from cucumber","authors":"Liuyi Dang, P. Rougé, E. Damme","doi":"10.16476/J.PIBB.2017.0311","DOIUrl":"https://doi.org/10.16476/J.PIBB.2017.0311","url":null,"abstract":"Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes which possess highly specific rRNA N-glycosidase activity and are capable of catalytically inactivating prokaryotic or eukaryotic ribosomes. Due to their unique biological activities, RIPs have been considered to have great potential in medical and agricultural applications. The cucumber genome accommodates two genes encoding type 2 ribosome-inactivating proteins, further referred to as CumsaAB1 and CumsaAB2. Type 2 RIPs, represented by ricin, usually consist of two peptides linked by a disulfide bridge. A chain with N-glycosidase activity and B chain with carbohydrate-binding activity. In this study, the expression of the cucumber RIPs was analyzed. Sequence analysis showed that CumsaAB1 is synthesized with a signal peptide and subcellular localization studies further confirmed that the protein is expressed extracellularly, following the secretory pathway. Analyses of the transcript levels in various tissues during cucumber development showed that CumsaAB1 is present at extremely low levels in most tissues while the expression of CumsaAB2 is much higher, especially in leaves from plants at first-true-leaf stage and plants at the onset of flowering. Molecular modelling of the RIP sequences was performed to unravel the three-dimensional conformation of cucumber RIPs and their carbohydrate-binding sites. This study provided valuable information on the subcellular localization, the tissue-specific expression and the structure of RIPs from cucumber plants.","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77847907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Endogenous electrical fields and its biological significance 内源性电场及其生物学意义

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2016-08-01 DOI: 10.16476/J.PIBB.2016.0100

D. Xiao-meng, G. Jing, S. Qin, W. Xiao-yan, S. Li-min, G. Run-chi, Z. Min, Z. San-jun

{"title":"Endogenous electrical fields and its biological significance","authors":"D. Xiao-meng, G. Jing, S. Qin, W. Xiao-yan, S. Li-min, G. Run-chi, Z. Min, Z. San-jun","doi":"10.16476/J.PIBB.2016.0100","DOIUrl":"https://doi.org/10.16476/J.PIBB.2016.0100","url":null,"abstract":"Progress in Biochemistry and Biophysics www.pibb.ac.cn ( 1) 云南师范大学生命科学学院，昆明 650500； 2) School of Medicine, University of California at Davis, California 95616, USA) Q64，Q424 早在 18 世纪，意大利生物学家 Galvani 偶然电 ” 的存在．1831 年， Matteucci 采用电流计 (galvanometer，以 Galvani 命名)在蛙腿神经和肌肉作电位的存在．随后德国生理学家 Du Bois Reymond 详细报道了生物电与神经刺激、肌肉收义，例如：电场直接诱导细胞各种行为．Pan 等 [3] DOI: 10.16476/j.pibb.2016.0100 (transepithelial potential，TEP)．极化的上皮细胞能上的 Na + -K + -ATP 酶将阳离子(主要是 Na + )运送至上皮细胞内侧，将阴离子(主要是 Cl - )运送至上皮细生物电信号”或“内源性电场(endogenous electrical field)”． Cao 等研究发现，内源性电场能够诱导高尔基体极化；Lobikin 等发现电场还能影响胚胎的发育过转人类间充质干细胞(hMSCs)的分化状态，诱导该 * 国家自然科学基金 - 云南省联合基金(U1132603)，国家重点基础研究发展计划(2012CB518100)和教育部留学回国人员科研启动基金 Tel: 0871-65943723, E-mail: sanjunzhao@hotmail.com","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74645496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Anticancer effects of valproic acid on esophageal stem-like carcinoma cells 丙戊酸对食管癌干样细胞的抗癌作用

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2016-07-12 DOI: 10.16476/J.PIBB.2016.0053

Saboor-Maleki, Saffiyeh, Rassouli, Fatemeh, Matin, Maryam, Mousavi

{"title":"Anticancer effects of valproic acid on esophageal stem-like carcinoma cells","authors":"Saboor-Maleki, Saffiyeh, Rassouli, Fatemeh, Matin, Maryam, Mousavi","doi":"10.16476/J.PIBB.2016.0053","DOIUrl":"https://doi.org/10.16476/J.PIBB.2016.0053","url":null,"abstract":"Valproic acid (VPA) is a histone deacetylase inhibitor that has been an object of interest to clinicians for its promising potency in cancer therapy, as it induces apoptosis and differentiation, and enhances of chemotherapy sensitivity. Esophageal squamous cell carcinoma (ESCC) is a malignant disease with growing incidence and low survival rate. Due to limited information on VPA activity in ESCC cells, we aimed to determine effects of VPA on chemotherapy responsiveness and expression of malignant markers in ESCC stem-like cells. Upon coadministration of non-toxic VPA + cisplatin (DDP), paclitaxel and 5-fluorouracil, viability of KYSE30 cells was assessed, and induced apoptosis was evaluated by DAPI staining, DNA laddering and flow cytometry. In addition, real time RT-PCR was performed to study changes in the expression of P21, CD44 and BMI-1 upon treatments. MTT test demonstrated that VPA significantly (P ＜ 0.05) increased toxicity of DDP, which was confirmed by DNA laddering, flow cytometry analysis and significant (P ＜ 0.05) overexpression of P21. Moreover, real time RT-PCR results indicated significant (P ＜ 0.05) down regulation of CD44 and BMI-1 after VPA administration. Present attempt provided evidence, for the first time, that VPA not only improved responsiveness of esophageal stem-like cancer cells to DDP, also negatively regulated cancer stem cells markers in these cells.","PeriodicalId":20655,"journal":{"name":"生物化学与生物物理进展","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2016-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85293549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mitochondrial damage induced by RNA binding proteins which are associated with neurodegenerative diseases 与神经退行性疾病相关的RNA结合蛋白诱导的线粒体损伤

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2016-04-01 DOI: 10.16476/J.PIBB.2016.0084

Li Zhu, Jianwen Deng, Peng Wang, Jianghong Liu, Jane Y. Wu

引用次数: 0

Neural mechanism of visual awareness in the human brain 人脑视觉意识的神经机制

IF 0.3 4区生物学

生物化学与生物物理进展 Pub Date : 2016-04-01 DOI: 10.16476/J.PIBB.2016.0118

Jiahan Hui, Linan Shi, Peng Zhang, Sheng He

引用次数: 0

Development and application of patch-clamp fluorometry in life science research 膜片钳荧光法在生命科学研究中的发展与应用