Photons Plus Ultrasound: Imaging and Sensing 2019最新文献_第3页

Targeted Sudan Black nanobubbles as photoacoustic contrast agents for breast cancer imaging (Conference Presentation) 靶向苏丹黑纳米气泡作为光声造影剂用于乳腺癌成像(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2510876

Yanjie Wang, E. Hysi, M. Moore, A. Leon, E. Abenojar, A. Exner, Michael C. Kolios

{"title":"Targeted Sudan Black nanobubbles as photoacoustic contrast agents for breast cancer imaging (Conference Presentation)","authors":"Yanjie Wang, E. Hysi, M. Moore, A. Leon, E. Abenojar, A. Exner, Michael C. Kolios","doi":"10.1117/12.2510876","DOIUrl":"https://doi.org/10.1117/12.2510876","url":null,"abstract":"Nanobubbles are a new class of ultrasound contrast agents. Unlike conventional microbubbles, their sub-micron (~200nm) diameter allows them to extravasate outside the vasculature and accumulate in the tumor interstitium. In this study, nanobubbles with shells loaded with Sudan Black (BNB) and DiD fluorescent dye were synthesized. These nanobubbles can be used to simultaneously enhance ultrasound and photoacoustic signals for in vivo breast tumor imaging. \u0000\u0000The nanobubbles consisted of lipid shells with a C3F8 gas core and were formed via self-assembly driven by mechanical agitation and size isolation via centrifugation. Herceptin antibody was conjugated to the BNB for targeting HER2-positive cells via standard EDC/NHS coupling chemistry. Human breast cancer cell lines (BT474 as HER2-positive and MDA-MB-231 as HER2-negative) were inoculated in the flanks of BALB/c-B17-Scid mice. Ultrasound and photoacoustic imaging (VevoLAZR, 21MHz, 720nm) were performed pre-injection and post-injection of the Herceptin conjugated BNB. The impact of Herceptin targeting was assessed by computing the PA frequency spectra and the non-linear contrast US images of the tumor regions. \u0000\u0000Photoacoustic images of the HER2-positive tumor showed an average of 6 dB increase in contrast signal 2 mins post-injection, while the HER2-negative MDA tumors showed a negligible change in image contrast, suggesting increased uptake of Herceptin labelled BNBs. The enhanced contrast is also confirmed by the non-linear contrast signals between positive and negative tumors. The photoacoustic technique can potentially be used to examine the kinetics of BNB extravasation. This work shows the potential of BNBs as multi-modal contrast agents capable of specialized tumor imaging in vivo.","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130497764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nonlinear oxygen saturation measurement for functional photoacoustic microscopy (Conference Presentation) 功能光声显微镜非线性氧饱和度测量(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2506952

Chao Liu, Yizhi Liang, Lidai Wang

{"title":"Nonlinear oxygen saturation measurement for functional photoacoustic microscopy (Conference Presentation)","authors":"Chao Liu, Yizhi Liang, Lidai Wang","doi":"10.1117/12.2506952","DOIUrl":"https://doi.org/10.1117/12.2506952","url":null,"abstract":"Optical-resolution photoacoustic microscopy can measure oxygen saturation () in vivo, offering an important tool to assess tissue oxygenation and health condition. Limited by available wavelengths for fast OR-PAM, the accuracy of sO2 imaging may be degraded by absorption saturation due to high absorption in the blood. Here, we report a nonlinear model to solve the saturation problem and increase the accuracy of measurement. The absorption saturation is analyzed by comparing a nonlinear and linear photoacoustic model using numerical simulation, which shows the nonlinear model has an improved accuracy than the linear model when the absorption is high. Phantom experiments on bovine blood further validate the accuracy of the nonlinear sO2 measurement method. In vivo experiments are conducted in the mouse ear. The values in a pair of arteries and veins are calculated using both linear and nonlinear methods, showing that the nonlinear method measures the arterial value closer to normal physiological condition than the conventional linear model. The nonlinear model requires the use of three or more wavelengths (532nm, 545nm, and 558nm in this work). As a result, we demonstrate the saturation effect in OR-PAM can be compensated via a nonlinear model, which may advance the application of functional optical-resolution photoacoustic microscopy.","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116715128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exponential path of photoacoustic tomography to the largest conference at Photonics West: omniscale imaging from organelles to organisms (Conference Presentation) 光声断层成像的指数路径到最大的Photonics West会议:从细胞器到生物体的全尺度成像(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2515432

Lihong V. Wang

{"title":"Exponential path of photoacoustic tomography to the largest conference at Photonics West: omniscale imaging from organelles to organisms (Conference Presentation)","authors":"Lihong V. Wang","doi":"10.1117/12.2515432","DOIUrl":"https://doi.org/10.1117/12.2515432","url":null,"abstract":"Photoacoustic tomography has been developed for in vivo functional, metabolic, molecular, and histologic imaging by physically combining optical and ultrasonic waves. Broad applications include early-cancer detection and brain imaging. High-resolution optical imaging—such as confocal microscopy, two-photon microscopy, and optical coherence tomography—is limited to superficial imaging within the optical diffusion limit (~1 mm in the skin) of the surface of scattering tissue. By synergistically combining light and sound, photoacoustic tomography conquers the optical diffusion limit and provides deep penetration at high ultrasonic resolution and high optical contrast. Photoacoustic tomography has two major embodiments: photoacoustic computed tomography and photoacoustic microscopy. In photoacoustic computed tomography, a pulsed broad laser beam illuminates the biological tissue to generate a small but rapid temperature rise, which leads to emission of ultrasonic waves due to thermoelastic expansion. The unscattered pulsed ultrasonic waves are then detected by ultrasonic transducers. High-resolution tomographic images of optical contrast are then formed through image reconstruction. In photoacoustic microscopy, a pulsed laser beam is delivered into the biological tissue to generate ultrasonic waves, which are then detected with a focused ultrasonic transducer to form a depth resolved 1D image. Raster scanning yields 3D high-resolution tomographic images. Super-depths beyond the optical diffusion limit have been reached with high spatial resolution. The annual conference on PAT has grown exponentially since early 2000 and become the largest in SPIE’s 20,000-attendee Photonics West since 2010.","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131896111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free non-contact imaging of cell nuclei using ultraviolet photoacoustic remote sensing microscopy (Conference Presentation) 利用紫外光声遥感显微镜进行细胞核无标签非接触成像(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2508036

Nathaniel J. M. Haven, K. Bell, Pradyumna Kedarisetti, P. H. Reza, J. Lewis, R. Zemp

{"title":"Label-free non-contact imaging of cell nuclei using ultraviolet photoacoustic remote sensing microscopy (Conference Presentation)","authors":"Nathaniel J. M. Haven, K. Bell, Pradyumna Kedarisetti, P. H. Reza, J. Lewis, R. Zemp","doi":"10.1117/12.2508036","DOIUrl":"https://doi.org/10.1117/12.2508036","url":null,"abstract":"Despite advancements in imaging and surgical methodology surgeons continue to face challenges in differentiating suitable margins in tumor resection sites and assessing the extent of cancer proliferation. Presently, histology is the gold standard used for determining margins post-operatively. However, currently no intraoperative tools can analyze entire resection sites. This results in unnecessary repeated surgeries and is especially critical to patient outcome for certain cancers. To address this critical need, we have developed a next-generation microscopy system intended to allow accurate intraoperative virtual histopathology for margin assessment. The modality, named ultraviolet photoacoustic remote sensing microscopy (UV-PARS), takes advantage of the intrinsic optical absorption contrast of DNA at 266 nm and a non-contact PARS technique. This approach measures time-dependent refractive index modulations at their subsurface origin resulting from thermo-elastic excitation from a pulsed excitation source. This enables the possibility of real time non-contact label-free visualization of cell nuclei in vivo. Preliminary results are presented including studies with live cell cultures, excised tissue samples, phantoms, and characterization of system parameters. Lateral-resolution was found to be 0.7 µm with a signal-to-noise ratio of 50 dB achieved in phantoms and 30 dB for HeLa cells. Simultaneously collected confocal reflectance microscopy using 1310 nm light provided cell body morphology. HeLa and PC3 cell cultures were imaged with good agreement to conventional H&E stained images of the same samples.","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133057141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Breast tumor appearances in photoacoustic tomography from fine 3D optical-acoustic simulations (Conference Presentation) 精细三维光声模拟的乳腺肿瘤光声断层扫描表现(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2509308

M. Dantuma, F. Lucka, J. Jaros, Bradley E. Teeby, B. Cox, S. Manohar

{"title":"Breast tumor appearances in photoacoustic tomography from fine 3D optical-acoustic simulations (Conference Presentation)","authors":"M. Dantuma, F. Lucka, J. Jaros, Bradley E. Teeby, B. Cox, S. Manohar","doi":"10.1117/12.2509308","DOIUrl":"https://doi.org/10.1117/12.2509308","url":null,"abstract":"Photoacoustic breast imaging has been under development for more than a decade now, and is progressively moving towards the clinics to investigate its performance in tumor detection and diagnosis. Several system types have been built, all with their own characteristics and corresponding imaging performances. Different studies have observed variations in tumor appearances. Some works have seen peripheral blood vessels in the tumor region, while others showed mass-like appearances at the tumor site. \u0000These varying tumor presentations may be caused by the deviations in system characteristics, but can also be caused by anatomical differences of the tumors. While we can still learn a lot from in-vivo studies, accurate simulation studies are needed to understand the tumor appearances in full detail.\u0000We have developed a simulation toolbox to perform 3D full breast photoacoustic simulations. To investigate the tumor appearance, several tumor models are embedded inside a MRI-segmented digital breast phantom [Y. Lou et al (2017)], which is pendant in a hemispherical imaging tank filled with water. Illumination fibers and US detectors are placed on the surface of the imaging bowl. The toolbox easily allows to adjust laser and detector characteristics. Illumination of the breast is simulated with GPU-accelerated Monte Carlo simulations [Q. Fang et al. (2009)]. The subsequent acoustic signal generation and propagation is modeled with k-wave (GPU-accelerated, [B.E. Treeby et al. (2010)]). Finally, images are reconstructed to evaluate the tumor appearance.","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"119 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130059493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Advancements in image reconstruction for photoacoustic computed tomography (Conference Presentation) 光声计算机断层成像重建的研究进展(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2516264

M. Anastasio

引用次数: 1

Image-guided photo-mediated ultrasound therapy as a novel method for treating eye diseases (Conference Presentation) 图像引导光介导超声治疗眼病的新方法(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2507680

Xinyi Xie, Yu Qin, Wei Zhang, Songtao Yuan, Qinghuai Liu, Qian Cheng, Y. Paulus, Xinmai Yang, Xueding Wang

{"title":"Image-guided photo-mediated ultrasound therapy as a novel method for treating eye diseases (Conference Presentation)","authors":"Xinyi Xie, Yu Qin, Wei Zhang, Songtao Yuan, Qinghuai Liu, Qian Cheng, Y. Paulus, Xinmai Yang, Xueding Wang","doi":"10.1117/12.2507680","DOIUrl":"https://doi.org/10.1117/12.2507680","url":null,"abstract":"Ocular neovascularization occurs in various eye diseases such as diabetic retinopathy, neovascular macular degeneration, and retinopathy of prematurity. Current treatment methods including conventional laser ablation therapy and anti-vascular endothelial growth factor (VEGF) injection each has drawbacks including collateral tissue damages, frequent administration, high cost, and drug toxicity. We recently developed a novel noninvasive image-guided photo-mediated ultrasound therapy (PUT) which concurrently applies nanosecond laser pulses and millisecond ultrasound bursts to precisely and safely remove pathologic microvessels in the eye. Relying on the mechanism of photoacoustic cavitation, PUT takes advantages of high optical contrast among biological tissues, and can selectively remove microvessels without causing collateral tissue damage.\u0000To achieve personalized treatment with optimal treatment outcome, a multi-modality eye imaging system involving advanced photoacoustic microscopy (PAM) and optical coherence tomography (OCT) has been integrated with the PUT system to provide real-time feedback and online evaluation of the treatment outcome. To assess the performance of this image-guided PUT system, experiments have been conducted on rabbit eye models. During the treatment, cavitation signals were observed and monitored by OCT with good sensitivity, suggesting that OCT can be used to evaluate treatment effect in real time. The PAM was capable of mapping the 3D distributed microvessels with excellent image quality, demonstrating that PAM can help to quantitatively evaluate the treatment outcome. As indicated by the initial results from this study, imaging guidance involving both PAM and OCT could further improve the efficacy and safety of the newly invented PUT, accelerating its translation to ophthalmology clinic.","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128533665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound point detectors in the silicon photonics platform for high frequency optoacoustic tomography (Conference Presentation) 高频光声层析成像硅光子平台中的超声点探测器(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2510712

Rami Shnaiderman, Georg Wissmeyer, H. Estrada, D. Razansky, Qutaiba Mustafa, Andriy Chmyrov, V. Ntziachristos

{"title":"Ultrasound point detectors in the silicon photonics platform for high frequency optoacoustic tomography (Conference Presentation)","authors":"Rami Shnaiderman, Georg Wissmeyer, H. Estrada, D. Razansky, Qutaiba Mustafa, Andriy Chmyrov, V. Ntziachristos","doi":"10.1117/12.2510712","DOIUrl":"https://doi.org/10.1117/12.2510712","url":null,"abstract":"Acoustic imaging modalities such as multispectral optoacoustic tomography (MSOT) have recently matured to a point which allows retrieval of anatomical, molecular and dynamic information with resolutions of several tens of microns\u0000These advancements are going hand in hand with the continuous development of ultrasound detectors. Yet, the traditional piezoelectric transducers have a distinct disadvantage: the detector size is proportional to its sensitivity. This limits miniaturization and prevents the development of point-like detectors as well as the subsequent construction of ultra-dense detector arrays. \u0000Consequently, research has shifted towards all-optical ultrasound detectors, such as Fabry-Perot resonators, where miniaturization does not affect detector sensitivity. In this context, we present a novel optical resonator on a silicon chip with a sensing area of 220 x 500 nm that - to the best of our knowledge – is the smallest ultrasound detector ever created. These dimensions are 77 and 34 times smaller than the acoustic wavelength at the central detection frequency, hence our detector is truly a point detector.\u0000Using the scalable silicon photonics platform we constructed an array of eight detectors. The archived density of 125 detectors/mm2 is larger by orders of magnitude compared to arrays of piezoelectric and capacitive micromachined ultrasound transducers.\u0000We describe the working principle of the detector and characterize its sensitivity, spatial response, and bandwidth. We demonstrate its applicability for optoacoustic tomography and perform the first SOI based tomography ever reported, by imaging micron-sized phantoms at light fluences well below the ANSI limit for human skin.","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128786546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered contrast agent platforms for enhanced photoacoustic signal and tumor uptake (Conference Presentation) 用于增强光声信号和肿瘤吸收的工程造影剂平台(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2507825

J. Joseph, Kevin N. Baumann, A. Vernet, S. Hernández-Ainsa, S. Bohndiek

{"title":"Engineered contrast agent platforms for enhanced photoacoustic signal and tumor uptake (Conference Presentation)","authors":"J. Joseph, Kevin N. Baumann, A. Vernet, S. Hernández-Ainsa, S. Bohndiek","doi":"10.1117/12.2507825","DOIUrl":"https://doi.org/10.1117/12.2507825","url":null,"abstract":"Photoacoustic imaging (PAI) can exploit near infrared (NIR) small molecule dyes to enhance contrast for the early identification of cancer with high biocompatibility and minimal toxicity. Unfortunately, the low optical absorption and rapid clearance of small molecule dyes impose considerable limitations for in vivo imaging applications, due to their limited PAI signal generation capabilities in the tumor bed. Using DNA nanotechnology it is possible to precisely position individual dye molecules to tune their intrinsic PAI signal generation capabilities and to engineer nano-carriers that enable targeted delivery and also prolong the lifetime of the contrast agent in vivo.\u0000Here, we report the synthesis, characterization and in vivo imaging of DNA nanotechnology-derived nano-carriers that exhibit superior tumor accumulation and photoacoustic (PA) signal generation capabilities when compared to free dyes. The nano-carrier (NC6Q+) comprised of closely positioned IRDye-800CW molecules offers good serum stability and biocompatibility. Absorption and emission measurements show that NC6Q+ exhibits a blue shifted absorption peak at 705nm due to exciton coupling and offers more than 80% fluorescence quenching efficiency. As a result, PAI data from NC6Q+ in tissue mimicking phantoms show a 72% enhancement in PA signal when compared to free IRDye-800CW. In vivo bio-distribution (n=2) and kinetics studies of the nano-carriers indicate 87 % increase in blood retention time compared to free dye, while maintaining renal clearance. Multispectral photoacoustic imaging of tumor bearing mice (n=3) showed 88% enhancement in tumor PA signal for NC6Q+ when compared to free IRDye-800CW. These results suggest that DNA nano-carriers hold promise to create PAI contrast agents with enhanced tumor uptake and PA signal generation capabilities","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114651127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoacoustic switching of methylene blue via interactions with sodium dodecyl sulfate (Conference Presentation) 亚甲基蓝与十二烷基硫酸钠相互作用的光声开关(会议报告)

Photons Plus Ultrasound: Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2508127

Junxin Wang, J. Jokerst

{"title":"Photoacoustic switching of methylene blue via interactions with sodium dodecyl sulfate (Conference Presentation)","authors":"Junxin Wang, J. Jokerst","doi":"10.1117/12.2508127","DOIUrl":"https://doi.org/10.1117/12.2508127","url":null,"abstract":"The interaction of methylene blue (MB) and sodium dodecyl sulfate (SDS) leads to a reversible spectral shift during SDS micellization, but the underlying mechanism has remained unclear. Here, we measured photoacoustic (PA) intensity, micelle concentration, and spectral shift of MB-SDS complex to elucidate this interaction mechanism. We observed a switchable PA effect of MB, which is sensitive to critical micelle concentration (CMC) of SDS (i.e. 8 mM). The addition of 3.47 mM SDS increased the PA intensity of 0.05 mM MB 492-fold because of fluorescence quenching. Then, the PA intensity decreased by 54-fold when the SDS concentration was increased above the CMC at 8.67 mM due to decrease of MB aggregation. Meanwhile, we observed increased number of non-micellar MB-SDS clusters, ranging from 80 to 400 nm, as the SDS concentration approaching to CMC and then the number decreased once the SDS concentration was above CMC. The correlation between PA intensity and nanoparticle number indicated that the formation of MB-SDS cluster was responsible for the PA enhancement. Further controlled studies using MB/hexadecyltrimethylammonium bromide, MB/sodium octyl sulfate, and MB/sodium chloride showed that the binding between MB and SDS occurred at the sulfate moiety of SDS. They also found that MB-SDS clusters disassociated to micelles MB-SDS monomers at the SDS micellar concentrations. These findings further elucidate the binding mechanism of MB and SDS and presented the potential for developing an activatable MB PA contrast agent.","PeriodicalId":206495,"journal":{"name":"Photons Plus Ultrasound: Imaging and Sensing 2019","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133836877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0