Proceedings of the National Science Council, Republic of China. Part B, Life sciences最新文献_第2页

Thermodynamic analysis of human retinal acetylcholinesterase inhibition using an anti-Alzheimer's drug, tacrine, through the development of a dual substrate and temperature model. 通过建立双底物和温度模型，对抗阿尔茨海默病药物他克林抑制人视网膜乙酰胆碱酯酶的热力学分析。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-07-01

M A Kamal, A S Alhomida, A A Al-Rajhi, A A Al-Jafari

{"title":"Thermodynamic analysis of human retinal acetylcholinesterase inhibition using an anti-Alzheimer's drug, tacrine, through the development of a dual substrate and temperature model.","authors":"M A Kamal, A S Alhomida, A A Al-Rajhi, A A Al-Jafari","doi":"","DOIUrl":"","url":null,"abstract":"The present study determines the energy parameters, such as the Gibb's free energy change (deltaG), enthalpy change (deltaH), heat of activation (deltaH*), entropy change (deltaS), temperature coefficient (Q10) and activation energy (Ea), of human retinal acetylcholinesterase (AChE, EC 3.1.1.7) inhibition by tacrine. The stereo-frequency collisions factor (PZ, the number of sterically and energetically favorable collisions occurring between tacrine and AChE) was also studied in this investigation. Tacrine significantly increased the value of deltaG, deltaH, deltaH*, Q10, Ea and PZ factor, and decreased the value of deltaS for AChE. Since there is no known report on the inhibition of human retinal AChE by tacrine, these results were compared with the reported values for the energy parameters of camel retinal and chicken brain AChE inhibition by an anti-cancer drug, cyclophosphamide. The uniqueness of this approach lies in the development of the 'dual substrate and dual temperature' model, which may open up a new, more efficient avenue for the study of various enzyme catalyzed reactions.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 3","pages":"108-15"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21782334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Central pool of serotonin and tail-flick latency during two phases of biphasic fever in rats. 大鼠两期双相发热时血清素中心池和甩尾潜伏期。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-07-01

S V Koulchitsky, G K Tropnikova, G P Mironova, Y A Pesotskaya, A N German, V A Kulchitsky

{"title":"Central pool of serotonin and tail-flick latency during two phases of biphasic fever in rats.","authors":"S V Koulchitsky, G K Tropnikova, G P Mironova, Y A Pesotskaya, A N German, V A Kulchitsky","doi":"","DOIUrl":"","url":null,"abstract":"In experiments on male Wistar rats, the acute phase reaction was induced by a bolus intravenous injection of Escherichia coli lipopolysaccharide (10 microg/kg) through a silicon catheter pre-implanted into the jugular vein. The colonic and skin temperature was measured with thermocouples. Changes in nociception were assessed based on tail flick latency (TFL) in response to a noxious heat stimulus. In this work, we observed the development of biphasic fever and phasic changes in TFL, namely, hyperalgesia in the first period of the acute phase reaction and hypoalgesia in its second phase. The catabolism of serotonin increased most considerably in the initial period of the acute phase reaction in the midbrain, striatum, and rostrodorsomedial medulla (on average, by 20-25%, 35-40%, and 95-100%, respectively). In the second phase of the acute phase reaction, despite a significant increase in the serotonin content in the striatum, midbrain, and cerebellum, there were no significant changes in serotonin catabolism in these parts of the CNS, which coincided with hypoalgesia. Thus, the phasic changes in TFL and colonic temperature after initiation of the acute phase reaction were accompanied by determinate changes in the catabolism of serotonin in different brain parts.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 3","pages":"123-8"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21782336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of a cell line (HCDB-1) derived from a hamster buccal pouch carcinoma induced by DMBA and Taiwanese betel quid extract. DMBA和台湾槟榔液提取物诱导的仓鼠颊袋癌HCDB-1细胞系的建立和表征。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-07-01

S C Lin, K W Chang, C S Chang, S Y Yu, S Y Chao, Y K Wong

引用次数: 0

Monitoring the changes of redox potential, pH and electrical conductivity of the mangrove soils in northern Taiwan. 台湾北部红树林土壤氧化还原电位、pH值及电导率变化之监测。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-07-01

Z Y Hseu, Z S Chen

{"title":"Monitoring the changes of redox potential, pH and electrical conductivity of the mangrove soils in northern Taiwan.","authors":"Z Y Hseu, Z S Chen","doi":"","DOIUrl":"","url":null,"abstract":"The redox potential (Eh), pH and electrical conductivity (EC) of the marsh soils of the Chuwei mangrove, located in the estuarine of the Tansui River in northern Taiwan were monitored for two years (from October 1995 to September 1997). The soils of selected pedons were studied, and the soils were classified based on Keys to Soil Taxonomy. The soil pH values tended to be neutral due to the impact of seawater on the mangrove marsh. The amounts of organic carbon found in this study area were much less than those generally encountered in the wetland soils of temperate regions in the world. The base saturation percentages of the soils were almost 100%, the exchangeable Na being particularly predominant. The concentrations of various cations of water in this ecosystem were in the order of Na+ > Mg2+ > K+ = Ca2+, and those of anions of water were in the order of Cl- > SO4(2-) > NO3- > PO4(3-). In spite of seasonal flooding changes, highly reduced states (100 to -200 mV of Eh values) existed throughout the two-year study. The spatial and temporal variations of the Eh values of the surface soil (0-20 cm) were higher than those of the subsoils (20-100 cm). The EC values of the soils from the surface to a depth of 100-cm were generally more than 20 dS/m. The marsh soils of the Chuwei mangrove were, thus, classified as Halic Endoaquents or Halic Fluvaquents.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 3","pages":"143-50"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21782339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of interleukin-2 receptor by activated peripheral blood lymphocytes upregulated by the plasma level of interleukin-2 in patients with recurrent aphthous ulcers. 复发性阿弗顿溃疡患者血浆白细胞介素-2水平上调活化外周血淋巴细胞白细胞介素-2受体的表达

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-07-01

A Sun, C T Chu, B Y Liu, J T Wang, J S Leu, C P Chiang

{"title":"Expression of interleukin-2 receptor by activated peripheral blood lymphocytes upregulated by the plasma level of interleukin-2 in patients with recurrent aphthous ulcers.","authors":"A Sun, C T Chu, B Y Liu, J T Wang, J S Leu, C P Chiang","doi":"","DOIUrl":"","url":null,"abstract":"This study used flow cytometry to determine the peripheral blood lymphocyte subsets and a sandwich enzyme immunoassay to measure the plasma levels of interleukin-2 (IL-2) and soluble IL-2 receptor (sIL-2R) in 34 patients in different stages of recurrent aphthous ulcers (RAU) and in 32 age/sex-matched normal control subjects. In the exacerbation stage of RAU, a significant increase in the percentages of CD3+ (p < 0.001), CD4+ (p < 0.001), CD4+ IL-2R+ (p < 0.001), CD8+ IL-2R+ (p < 0.01) and IL-2R+ cells (p < 0.001), in the CD4+/CD8+ (p < 0.01) and CD4+/CD3+ CD8+ ratios (p < 0.01), and in the plasma level of IL-2 (p < 0.001) was found in the patients as compared with the levels in the normal control subjects. However, in the post-exacerbation stage of RAU, there was a significant decrease in the percentage of CD4+ cells (p < 0.05) and in the CD4+/CD8+ (p < 0.01) and CD4+/CD3+ CD8+ ratios (p < 0.001), as well as a significant increase in CD8+ cells (p < 0.001) in the patients, as compared with the levels in the normal control subjects. Because the CD4+, CD4+ IL-2R+ and CD8+ IL-2R+ cell counts and the plasma level of IL-2 increased simultaneously in the patients in the exacerbation stage of RAU, we suggest that the markedly increased plasma level of IL-2 may have been secreted by the increased number of activated CD4+ cells, and that the expression of IL-2R by activated peripheral blood lymphocytes was upregulated by the plasma level of IL-2 in patients with RAU. In addition, the increase and then decrease of the CD4+/CD8+ ratio in the RAU patients and the increased number of CD4+ IL-2R+ and CD8+ IL-2R+ activated T cells in the RAU patients support the role of cell-mediated cytotoxicity in the immunopathogenesis of RAU.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 3","pages":"116-22"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21782335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A model and its implications for denitrification in soil environment. 土壤环境反硝化模型及其意义。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-07-01

C Lin, C Chen, W Hsiang, T L Hu

{"title":"A model and its implications for denitrification in soil environment.","authors":"C Lin, C Chen, W Hsiang, T L Hu","doi":"","DOIUrl":"","url":null,"abstract":"We analyzed the mechanisms of a soil nitrogen (N) sub-model, which is a subroutine of the Crop-Environment Resources Synthesis (CERES)-maize; a model which was originally designed to simulate crop yield and has been calibrated and validated in Taiwan. Some experiments designed specifically for testing the N sub-model proved the capability of the model in reflecting field observations. With the mechanisms, we could write computer programs for calculating the relative sensitivities of major parameters in the model, and for simulating different treatments of organic matter. The purposes were to find how they affected N transformations, especially the processes of denitrification, which are considered to be responsible for N losses in upland soils and are an important environmental issue related to human disturbance of the N cycle. The results implied that soil water content and temperature were, respectively, the first and second dominant factors. They were much more sensitive than any other parameters, such as the decomposition rate coefficients, soil pH and bulk density. Decomposition of organic matter could slow down if organic matter with different carbon/nitrogen (C/N) ratios were treated in fractions. This treatment could also decrease the process of denitrification unless the organic matter was extremely large in quantity and has a high C/N ratio.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 3","pages":"136-42"},"PeriodicalIF":0.0,"publicationDate":"2000-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21782338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of HLA-te22 antigen with anti-nuclear antibodies in Chinese patients with erosive oral lichen planus. 中国口腔糜烂性扁平苔藓患者HLA-te22抗原与抗核抗体的关系。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-04-01

A Sun, Y C Wu, J T Wang, B Y Liu, C P Chiang

{"title":"Association of HLA-te22 antigen with anti-nuclear antibodies in Chinese patients with erosive oral lichen planus.","authors":"A Sun, Y C Wu, J T Wang, B Y Liu, C P Chiang","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was to determine the frequencies of the presence of serum anti-nuclear antibodies (ANA) and smooth muscle antibodies (SMA) in 76 patients with oral lichen planus (OLP), in 77 patients with other oral mucosal diseases, and in 41 healthy control subjects. HLA phenotypes in some of the patients with OLP and recurrent aphthous ulcers (RAU) were determined to show whether there was an association of HLA antigens with the presence of autoantibodies. Indirect immunofluorescence techniques with mouse liver or stomach as the substrate were used to detect the serum ANA or SMA, respectively. The B lymphocytes isolated from peripheral blood were used for HLA typing by means of a standard microcytotoxicity assay. We found that the positive rate of serum ANA in patients with OLP (29%, p < 0.01), especially in patients with erosive OLP (34%, p < 0.001), was significantly higher than that in the normal control subjects (5%). The frequency of serum SMA in patients with OLP (20%, p < 0.01), in patients with RAU (17%, p < 0.01), or in patients with oral squamous cell carcinomas (41%, p < 0.001) was also significantly higher than that in normal control individuals (0%). In the erosive OLP group, the HLA-Te22 antigen occurred more frequently in patients with positive ANA (75%, p < 0.05) than in those with negative ANA (25%). We conclude that there is an association of HLA-Te22 antigen with ANA in Chinese patients with erosive OLP.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 2","pages":"63-9"},"PeriodicalIF":0.0,"publicationDate":"2000-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21655811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Astrocytes modulate thapsigargin-induced changes in calcium concentration and neuronal survival. 星形胶质细胞调节信号素诱导的钙浓度变化和神经元存活。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-04-01

C J Yao, C W Lin, S Y Lin-Shiau

{"title":"Astrocytes modulate thapsigargin-induced changes in calcium concentration and neuronal survival.","authors":"C J Yao, C W Lin, S Y Lin-Shiau","doi":"","DOIUrl":"","url":null,"abstract":"When mature cerebellar granule neurons (CGN) grown in high K+ (25 mM K+, HK)-serum containing medium are subjected to the HK/serum deprivation, they are destined for neuronal death. In this study, we attempted to elucidate the roles of endoplasmic reticular (ER) Ca2+-store and co-cultured astrocytes in HK/serum deprivation induced neuronal death. Thapsigargin (TG), an inhibitor of ER Ca2+-ATPase was simultaneously applied with normal K+ (5 mM K+, NK) serum free medium, and its effects on neuronal death in either astrocyte-poor or astrocyterich culture were examined. By means of the fura-2 microfluorimetric technique, we monitored the changes of the intracellular Ca2+ concentration, [Ca2+]i, associated with neuronal death under various treatments. The results obtained showed that in astrocyte-poor cultures of mature CGN (10 days in vitro, DIV), the basal level of [Ca2+]i markedly decreased from 184 +/- 5 to 89.7 +/- 5 nM 24 h after HK/serum deprivation. Although treatment with TG slightly increased the [Ca2+]i to 117.6 +/- 4 nM, the survival rate of the neurons was even worse; it was reduced from 49 +/- 4% to 28 +/- 2%. In the astrocyte-rich cultures, HK/serum deprivation also caused a profound reduction of neuronal [Ca2+]i, from 166 +/- 3 to 90.2 +/- 6 nM, accompanied by even more serious neuronal death (95.5 +/- 1%). On the other hand, treatment with TG in astrocyterich cultures further lowered the [Ca2+]i to 65 +/- 2 nM but markedly improved the neuronal survival rate from 4.5 +/- 1% to 60 +/- 2% in a concentration-dependent manner. The strong implication of these findings is that ER Ca2+-store and astrocytes participate in modulating the responses of neurons to stress stimulation.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 2","pages":"81-7"},"PeriodicalIF":0.0,"publicationDate":"2000-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21655814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and function of the Groucho gene family and encoded transcriptional corepressor proteins from human, mouse, rat, Xenopus, Drosophila and nematode. 人、小鼠、大鼠、爪蟾、果蝇和线虫中Groucho基因家族的结构、功能和编码转录共抑制蛋白。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-04-01

S S Li

{"title":"Structure and function of the Groucho gene family and encoded transcriptional corepressor proteins from human, mouse, rat, Xenopus, Drosophila and nematode.","authors":"S S Li","doi":"","DOIUrl":"","url":null,"abstract":"A gene family of the Groucho, TLE, ESG and AES proteins has been characterized from Drosophila, nematode, Xenopus, mouse, rat and human, and their structural relationships have been analyzed. The genomic organization of nematode ESG, human and mouse AES genes has been determined, and the expression of ESG and AES genes from Xenopus and human has been analyzed. The Groucho, TLE and ESG proteins all share a similar structure, consisting of a conserved amino-terminal domain, a variable middle region, and highly conserved carboxyl-terminal WD-40 repeats. The Drosophila Groucho transcriptional corepressor protein has been shown to interact with the DNA-binding bHLH domain of Enhancer of split, Hairy and Deadpan proteins, which proteins are involved in neurogenesis, segmentation and sex-determination, respectively. Human TLE1 protein has been demonstrated to interact with mammalian AML1 protein, which regulates hematopoiesis and osteoblast differentiation. The AES proteins from human, mouse, rat and Xenopus exhibit strong similarity to the amino-terminal domain of Groucho proteins; however, the biological function remains to be elucidated.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 2","pages":"47-55"},"PeriodicalIF":0.0,"publicationDate":"2000-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21655809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Milk progesterone profiles in various reproductive states in dairy buffaloes under field conditions. 田间条件下不同繁殖状态下乳水牛的乳黄体酮谱。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 2000-04-01

M S Qureshi, G Habib, G Nawab, M M Siddiwqui, N Ahmad, H A Samad

{"title":"Milk progesterone profiles in various reproductive states in dairy buffaloes under field conditions.","authors":"M S Qureshi, G Habib, G Nawab, M M Siddiwqui, N Ahmad, H A Samad","doi":"","DOIUrl":"","url":null,"abstract":"Fifty-one dairy buffaloes in the last two months of gestation were selected at seven private peri-urban farms in the Peshawar district. Observations were recorded in buffaloes during normal (NBS, August to January) and low breeding seasons (LBS, February to July). After parturition, rectal examination of reproductive organs was carried out. Estrus detection was made through visual observation and the use of intact bull. Postpartum ovulation was confirmed by ovarian palpation per rectum and milk progesterone levels (MPL), determined through radio-immunoassay. MPL was higher (p < 0.01) at various intervals in NBS calves (1.97 +/- 0.30 ng/ml) as compared to LBS calves (0.68 +/- 0.08 ng/ml). During LBS, MPL remained < 0.30 ng/ml up to the third fortnight and started rising later, reaching a peak of 1.27 ng/ml during the sixth fortnight. During NBS, there was a sharp rise in MPL during the second fortnight, reaching 3.64 ng/ml during the sixth fortnight. MPL was significantly different on different experimental farms (p < 0.01). MPL reached the lowest levels on the day of estrus (0.10 ng/ml), reached it's peak on day 7 and started declining on day 17 of estrus. MPL showed two postpartum elevations. In true anestrus buffaloes, MPL remained consistently low. However, in the anestrus period, silent ovulations were also noted, as reflected by increasing MPL without estrus signs. In pregnant buffaloes, MPL remained > 1 ng/ml. Results of the study showed that the low postpartum reproductive performance in dairy buffaloes during LBS was primarily due to inadequate functioning of the corpus luteum in secreting optimum concentrations of progesterone. The higher incidence of silent estrus during LBS indicated improved management for the detection of estrus.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"24 2","pages":"70-5"},"PeriodicalIF":0.0,"publicationDate":"2000-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21655812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0