Expression of interleukin-2 receptor by activated peripheral blood lymphocytes upregulated by the plasma level of interleukin-2 in patients with recurrent aphthous ulcers.

A Sun, C T Chu, B Y Liu, J T Wang, J S Leu, C P Chiang
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Abstract

This study used flow cytometry to determine the peripheral blood lymphocyte subsets and a sandwich enzyme immunoassay to measure the plasma levels of interleukin-2 (IL-2) and soluble IL-2 receptor (sIL-2R) in 34 patients in different stages of recurrent aphthous ulcers (RAU) and in 32 age/sex-matched normal control subjects. In the exacerbation stage of RAU, a significant increase in the percentages of CD3+ (p < 0.001), CD4+ (p < 0.001), CD4+ IL-2R+ (p < 0.001), CD8+ IL-2R+ (p < 0.01) and IL-2R+ cells (p < 0.001), in the CD4+/CD8+ (p < 0.01) and CD4+/CD3+ CD8+ ratios (p < 0.01), and in the plasma level of IL-2 (p < 0.001) was found in the patients as compared with the levels in the normal control subjects. However, in the post-exacerbation stage of RAU, there was a significant decrease in the percentage of CD4+ cells (p < 0.05) and in the CD4+/CD8+ (p < 0.01) and CD4+/CD3+ CD8+ ratios (p < 0.001), as well as a significant increase in CD8+ cells (p < 0.001) in the patients, as compared with the levels in the normal control subjects. Because the CD4+, CD4+ IL-2R+ and CD8+ IL-2R+ cell counts and the plasma level of IL-2 increased simultaneously in the patients in the exacerbation stage of RAU, we suggest that the markedly increased plasma level of IL-2 may have been secreted by the increased number of activated CD4+ cells, and that the expression of IL-2R by activated peripheral blood lymphocytes was upregulated by the plasma level of IL-2 in patients with RAU. In addition, the increase and then decrease of the CD4+/CD8+ ratio in the RAU patients and the increased number of CD4+ IL-2R+ and CD8+ IL-2R+ activated T cells in the RAU patients support the role of cell-mediated cytotoxicity in the immunopathogenesis of RAU.

复发性阿弗顿溃疡患者血浆白细胞介素-2水平上调活化外周血淋巴细胞白细胞介素-2受体的表达
本研究采用流式细胞术测定34例复发性阿弗特溃疡(RAU)不同分期患者的外周血淋巴细胞亚群,并采用夹心酶免疫分析法测定血浆白细胞介素-2 (IL-2)和可溶性IL-2受体(sIL-2R)水平,并与32例年龄/性别匹配的正常对照。RAU加重期患者CD3+、CD4+ (p < 0.001)、CD4+ IL-2R+ (p < 0.001)、CD8+ IL-2R+ (p < 0.01)、IL-2R+细胞百分比、CD4+/CD8+ (p < 0.01)、CD4+/CD3+ CD8+比值、血浆IL-2水平均较正常对照组显著升高(p < 0.001)。然而,在RAU加重后阶段,患者CD4+细胞百分比(p < 0.05)、CD4+/CD8+比值(p < 0.01)和CD4+/CD3+ CD8+比值(p < 0.001)明显低于正常对照组,CD8+细胞水平明显高于正常对照组(p < 0.001)。由于RAU加重期患者CD4+、CD4+ IL-2R+和CD8+ IL-2R+细胞计数及血浆IL-2水平同时升高,我们认为血浆IL-2水平的显著升高可能是由于活化的CD4+细胞数量增加而分泌的,激活的外周血淋巴细胞IL-2R的表达被RAU患者血浆IL-2水平上调。此外,RAU患者CD4+/CD8+比值先升高后降低,RAU患者CD4+ IL-2R+和CD8+ IL-2R+活化T细胞数量增加,支持细胞介导的细胞毒性在RAU免疫发病中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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