Proceedings of the National Science Council, Republic of China. Part B, Life sciences最新文献_第10页

Growth characteristics of normal and dystrophic myoblasts in primary myoblast cultures. 原代成肌细胞培养中正常和营养不良成肌细胞的生长特性。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1996-04-01

K F Cheng, Y H Chuang, W Y Her, S C Chen, K M Liu

{"title":"Growth characteristics of normal and dystrophic myoblasts in primary myoblast cultures.","authors":"K F Cheng, Y H Chuang, W Y Her, S C Chen, K M Liu","doi":"","DOIUrl":"","url":null,"abstract":"This study employed immunocytochemistry and toluidine blue counterstaining to compare different procedures utilized in primary myoblast cultures, from which an optimal culture model for normal myoblasts could be derived. The growth characteristics of normal and dystrophic myoblasts were also investigated by means of this model. Results indicate that the requirements for an ideal myoblast culture should include a combined enzyme of 0.25% trypsin and 0.2% collagenase (type IV) (1:1), a preplating time of approximately 15-20 minutes, and a seeding density of 1 x 10(5) cells/ml. Furthermore, the mouse samples should be newborn mice. A better proliferative capacity of myoblasts was noted in an incubator with 10% CO2 coupled with Dulbecco's MEM plus 15% fetal calf serum. With regard to the growth characteristics of normal and dystrophic myoblasts, the doubling time of normal myoblasts was shorter than that of dystrophic myoblasts. In terms of the fusion percentage of myoblasts, dystrophic myoblasts tended to fuse earlier than normal ones, especially after 5 days in culture. The findings of this study are valuable in understanding the myogenesis of myoblasts under different culture conditions. The establishment of requirements for good growth of myoblast cultures will facilitate myoblast transfer therapy. Finally, the growth characteristics of normal and dystrophic myoblasts as well as variances in the proliferation and differentiation of these two types of cells are clarified.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19896138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

X-ray structure determination of antineoplastic agent 2-chloromethyl-1,2,4-triazolo [5,1-b] [1.3]-benzothiazin-9-one. 抗肿瘤药物 2-氯甲基-1,2,4-三唑并[5,1-b] [1.3]- 苯并噻嗪-9-酮的 X 射线结构测定。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1996-04-01

Tian-Huey Lu, Tahir Hajioglu Tahirov, Kang Chien Liu, Bi-Jane Shih, Ling Yih Hsu

引用次数: 0

Clinical significance of complements in ascitic diseases: elevated complement levels disapproving the liver disease origin. 补体在腹水疾病中的临床意义:补体水平升高不利于肝脏疾病的起源。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1996-04-01

S S Wang, F Y Lee, Y Chao, C C Chen, H Y Lin, S L Wu, S D Lee

{"title":"Clinical significance of complements in ascitic diseases: elevated complement levels disapproving the liver disease origin.","authors":"S S Wang, F Y Lee, Y Chao, C C Chen, H Y Lin, S L Wu, S D Lee","doi":"","DOIUrl":"","url":null,"abstract":"In a two-year period, ascitic fluid concentrations of complement 3c and complement 4 were measured in 110 patients with sterile cirrhotic ascites, 31 patients with spontaneous bacterial peritonitis, 65 patients with hepatocellular carcinoma, 36 patients with peritoneal carcinomatosis and 12 patients with miscellaneous diseases (nephrotic syndrome 4, systemic lupus erythematosus 3, secondary peritonitis 2, cardiac ascites 1, eosinophilic peritonitis 1 and tuberculosis peritonitis 1) to assess the clinical utility of ascitic fluid complements. The ascitic fluid level of complements 3c or C4 was significantly higher in patients with peritoneal carcinomatosis (32.8 +/- 10.2, 13.4 +/- 7.4 mg/dL) than in patients with sterile cirrhotic ascites (9.2 +/- 5.2, 4.5 +/- 3.9 mg/dL, p < 0.001), spontaneous bacterial peritonitis (8.2 +/- 4.1, 3.8 +/- 2.4 mg/dL, p < 0.001) or hepatocellular carcinoma (12.8 +/- 8.3, 5.6 +/- 4.4 mg/dL, p < 0.001). However, it was not significantly different from the miscellaneous disease group. To verify that ascites formation is not related to liver disease origin, diagnostic sensitivity, specificity and accuracy were 83.3%, 92.7% and 90.9%, respectively, by the ascitic fluid level of complement 3c higher than the cut-off value (20 mg/dl); or 60.4%, 89.8% and 84.3%, respectively, by the ascitic fluid level of complement 4 higher than the cut-off value (10 mg/dL). A direct correlation was found between the ascitic fluid protein level and the ascitic fluid complement 3c (r = 0.70) or complement 4 (r = 0.57) level. Based on results in this study, we can conclude that measuring ascitic fluid complements is clinically useful in disapproving the liver disease origin of ascites formation. However, it is of little value in diagnosing spontaneous bacterial peritonitis or hepatocellular carcinoma.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19895391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-step hybridization screening for recombinant DNA clones with correct insert orientation and intact junction using a junctional oligonucleotide probe. 使用连接寡核苷酸探针对具有正确插入方向和完整连接的重组DNA克隆进行单步杂交筛选。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1996-04-01

L C Au, T Y Lo, H H Wang, K B Choo

引用次数: 0

Effects of captopril on renal functions, renal and portal hemodynamics in patients with cirrhosis. 卡托普利对肝硬化患者肾功能、肾脏及门静脉血流动力学的影响。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1996-04-01

Y T Tsai, H C Lin, F Y Lee, M C Hou, S S Wang, S D Lee

{"title":"Effects of captopril on renal functions, renal and portal hemodynamics in patients with cirrhosis.","authors":"Y T Tsai, H C Lin, F Y Lee, M C Hou, S S Wang, S D Lee","doi":"","DOIUrl":"","url":null,"abstract":"Renin-angiotensin system plays a prominent role in the sodium and water homeostasis. In addition, activation of renin-angiotensin system frequently occurs in patients with cirrhosis and ascites. Theoretically, administering of angiotensin converting enzyme inhibitors can enhance sodium and water retention in cirrhotic patients with ascites. In this study, we evaluate the role of low-dose captopril on renal function changes, renal plasma flow and hemodynamics in patients with cirrhosis and ascites. Fifty patients are randomly assigned to receive captopril or placebo for 14 days. Renal functions, renal plasma flow, plasma renin activity, plasma aldosterone concentration and systemic and hepatic hemodynamics are measured before and after treatment. Our results indicate that placebo administration did not affect any of the parameter measured in this study. The finding that low-dose captopril significantly increases plasma renin activity suggests that the dose used in this study effectively blocks the enzyme activity. However, low-dose captopril does not affect renal plasma flow, renal functions and systemic and hepatic hemodynamics. Results in this study demonstrate that inhibition of angiotensin converting enzyme alone may not improve sodium and water retention in cirrhotic patients with ascites.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19896141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of thymic hormones in thymic tumors and myasthenic thymus. 胸腺肿瘤与肌无力胸腺中胸腺激素的分布。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1996-01-01

M H Wu, T L Low

{"title":"Distribution of thymic hormones in thymic tumors and myasthenic thymus.","authors":"M H Wu, T L Low","doi":"","DOIUrl":"","url":null,"abstract":"To understand the thymic hormone production in thymic tumors and the myasthenic thymus, we have studied the concentrations of two thymic hormones (prothmosin alpha and thymosin beta 4) in these tissues. A total of forty four thymus or thymic tumor tissues were obtained from patients who underwent thoracic operation. These tissues consisted of a control group (n = 25) and three study groups including a thymoma group (N = 8), thymic carcinoma group (n = 3) and myasthenic thymus group (n = 8). The age-related curve of the control group, which provided histologically normal thymic tissues for the production of prothymosin alpha and thymosin beta 4, was established. It was found that in the control group the concentrations (microgram/g tissue) of prothymosin alpha and thymosin beta 4 decreased with age, and that the ranges were from 171 micrograms/g to trace and from 243 micrograms/g to trace, respectively. The amounts were highest at puberty and then gradually decreased, and only trace amounts of both polypeptides were present at age 60. Study cases which deviated from the standard curves were identified. Our results indicated that the contents of thymic hormones significantly increased in the tissues of invasive or non-invasive thymoma, decreased in thymic carcinoma, and were not significantly changed in myasthenic thymus as compared with those of the age-related normal thymus tissues. These findings confirm the notion that only thymomas produce significantly higher thymic hormones.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19737124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alterations of remnant liver carnitine and serum carnitine concentrations after partial hepatectomy in rats. 大鼠肝部分切除后残肝肉碱和血清肉碱浓度的变化。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1996-01-01

Y Chen, H S Lai, W J Chen

{"title":"Alterations of remnant liver carnitine and serum carnitine concentrations after partial hepatectomy in rats.","authors":"Y Chen, H S Lai, W J Chen","doi":"","DOIUrl":"","url":null,"abstract":"The role of carnitine in the early phase of liver regeneration after partial hepatectomy has been a controversial issue. The purpose for this study was to evaluate the alterations of remnant liver carnitine and serum carnitine concentrations in partial-hepatectomized rats. Partial hepatectomy, with resection of the median and left lateral lobes (67%), was done in 40 male Wistar rats weighing about 200 g. Another group of 40 rats underwent a sham-operation for comparison. All the rats underwent fasting for 4 hours before surgical procedure and 2 hours after surgery. They were killed at 6, 24, 48 or 72h after the operation, ten rats for each time. The serum carnitine concentration, remnant liver carnitine content, serum free fatty acid (FFA), acetoacetate (AA) and beta-hydroxybutyrate (BB) concentrations were measured. It was found that: (1) the serum carnitine concentration increased in the early posthepatectomized stage; (2) the carnitine content in the remnant liver increased in the early posthepatectomized stage; and (3) the serum FFA and its metabolites markedly increased with a depressed ketone body ratio noted in the early post-hepatectomized stage. These results supported the theory that regenerating liver utilized free fatty acids as an immediate main substrate. The utilization then decreased. Because of a marked increase of the carnitine concentration in the remnant liver and serum, it is suggested that the carnitine contents were sufficient for liver regeneration. There must be other reasons for the decreased utilization of free fatty acids.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19737125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of lytic enzyme from Pseudomonas aeruginosa M-1001. 铜绿假单胞菌M-1001裂解酶的生产。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1995-10-01

S L Wang, C S Pai, S T Shieh

引用次数: 0

Production of the third component of complement (C3) by peripheral polymorphonuclear neutrophils of the patients with rheumatoid arthritis. 类风湿关节炎患者外周血多形核中性粒细胞产生补体第三组分(C3)。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1995-10-01

C L Yu, C Y Tsai, S C Hsieh, Y Y Tsai, S T Tsai, K H Sun, H S Yu, S H Han

引用次数: 0

Mitochondrial alterations of skeletal muscle in a heat stress rat model. 热应激大鼠模型骨骼肌线粒体的改变。

Proceedings of the National Science Council, Republic of China. Part B, Life sciences Pub Date : 1995-10-01

Y D Hsu, S S Chen, W H Lee, S Z Lin, M C Kao, W L Tsao

{"title":"Mitochondrial alterations of skeletal muscle in a heat stress rat model.","authors":"Y D Hsu, S S Chen, W H Lee, S Z Lin, M C Kao, W L Tsao","doi":"","DOIUrl":"","url":null,"abstract":"The morphological and ultrastructural alterations of skeletal muscle in experimental rats with heat stress were investigated. Fifteen male Sprague-Dawley rats were exposed in a 42 degrees C constant temperature oven, resulting in a heat stress state; ten rats were used as controls. All treated rats had weakness of the 4 limbs associated with increased serum creatine kinase levels (p < 0.01). Soleus muscles were submitted to histological, histochemical, ultrastructural and quantitative-morphometric analysis. The group receiving heat stress showed many ragged-red fibers in Gomori trichrome stain and appeared hyper-reactive in succinate dehydrogenase and cytochrome C oxidase stains. The ultrastructure of ragged-red fibers showed increased mitochondrial aggregation as multiple small nests, which were particularly located in the subsarcolemmal space. The mitochondrial area was significantly increased in heat stress rats (p < 0.001). The consistently increased mitochondrial area and histochemical alterations of mitochondria are early pathological abnormalities in muscles with heat stress and indicate fundamental impairment of energy metabolism.","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19716159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0