铜绿假单胞菌M-1001裂解酶的生产。

S L Wang, C S Pai, S T Shieh
{"title":"铜绿假单胞菌M-1001裂解酶的生产。","authors":"S L Wang,&nbsp;C S Pai,&nbsp;S T Shieh","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Pseudomonas aeruginosa M-1001 produced both hen-egg-white lysozyme inhibitors and lytic enzyme in the culture broth. The lytic enzyme produced was not inhibited by the lysozyme inhibitors. Maximal lytic activity was obtained when the strain was grown aerobically in a medium consisting of 0.25% glucose, 0.75% soluble strach, 0.25% beef extract, 0.25% Polypepton, 0.25% sodium L-glutamate and 0.0001% NaCl (pH 6), at 37 degrees C after 36 hrs. The lytic enzyme was stable at pH from 6 to 8 and temperatures below 40 degrees C. Many bacteria and Saccharomyces cerevisiae were found to be inhibited by the crude lytic enzyme produced from the M-1001 strain except for the strain itself and a few other microorganisms.</p>","PeriodicalId":20569,"journal":{"name":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","volume":"19 4","pages":"216-24"},"PeriodicalIF":0.0000,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Production of lytic enzyme from Pseudomonas aeruginosa M-1001.\",\"authors\":\"S L Wang,&nbsp;C S Pai,&nbsp;S T Shieh\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Pseudomonas aeruginosa M-1001 produced both hen-egg-white lysozyme inhibitors and lytic enzyme in the culture broth. The lytic enzyme produced was not inhibited by the lysozyme inhibitors. Maximal lytic activity was obtained when the strain was grown aerobically in a medium consisting of 0.25% glucose, 0.75% soluble strach, 0.25% beef extract, 0.25% Polypepton, 0.25% sodium L-glutamate and 0.0001% NaCl (pH 6), at 37 degrees C after 36 hrs. The lytic enzyme was stable at pH from 6 to 8 and temperatures below 40 degrees C. Many bacteria and Saccharomyces cerevisiae were found to be inhibited by the crude lytic enzyme produced from the M-1001 strain except for the strain itself and a few other microorganisms.</p>\",\"PeriodicalId\":20569,\"journal\":{\"name\":\"Proceedings of the National Science Council, Republic of China. Part B, Life sciences\",\"volume\":\"19 4\",\"pages\":\"216-24\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the National Science Council, Republic of China. Part B, Life sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the National Science Council, Republic of China. Part B, Life sciences","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

铜绿假单胞菌M-1001在培养液中产生蛋清溶菌酶抑制剂和溶菌酶。所产生的溶菌酶不受溶菌酶抑制剂的抑制。菌株在含0.25%葡萄糖、0.75%可溶性淀粉、0.25%牛肉提取物、0.25%多肽、0.25% l -谷氨酸钠和0.0001% NaCl (pH 6)的培养基中37℃培养36小时,获得最大的酶解活性。该酶在pH为6 ~ 8、温度低于40℃时稳定,除M-1001菌株本身和少数其他微生物外,许多细菌和酿酒酵母菌都被该酶所抑制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Production of lytic enzyme from Pseudomonas aeruginosa M-1001.

Pseudomonas aeruginosa M-1001 produced both hen-egg-white lysozyme inhibitors and lytic enzyme in the culture broth. The lytic enzyme produced was not inhibited by the lysozyme inhibitors. Maximal lytic activity was obtained when the strain was grown aerobically in a medium consisting of 0.25% glucose, 0.75% soluble strach, 0.25% beef extract, 0.25% Polypepton, 0.25% sodium L-glutamate and 0.0001% NaCl (pH 6), at 37 degrees C after 36 hrs. The lytic enzyme was stable at pH from 6 to 8 and temperatures below 40 degrees C. Many bacteria and Saccharomyces cerevisiae were found to be inhibited by the crude lytic enzyme produced from the M-1001 strain except for the strain itself and a few other microorganisms.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信