大鼠肝部分切除后残肝肉碱和血清肉碱浓度的变化。

Y Chen, H S Lai, W J Chen
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引用次数: 0

摘要

肉碱在部分肝切除术后肝再生早期的作用一直是一个有争议的问题。本研究的目的是评价部分肝切除大鼠残肝肉碱和血清肉碱浓度的变化。40只体重约200 g的雄性Wistar大鼠行部分肝切除术,切除正中叶和左外侧叶(67%)。另一组40只大鼠进行了假手术进行比较。所有大鼠术前禁食4小时,术后禁食2小时。分别于术后6、24、48、72h处死,每次10只。测定血清肉碱浓度、残肝肉碱含量、血清游离脂肪酸(FFA)、乙酰乙酸(AA)和β -羟基丁酸(BB)浓度。结果发现:(1)术后早期血清肉碱浓度升高;(2)残肝肉碱含量在切除后早期升高;(3)肝切除后早期血清FFA及其代谢物显著升高,酮体比例下降。这些结果支持了肝脏再生利用游离脂肪酸作为直接主要底物的理论。然后利用率下降。由于残肝和血清中肉碱浓度显著升高,提示肉碱含量足以促进肝脏再生。游离脂肪酸利用率下降一定有其他原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alterations of remnant liver carnitine and serum carnitine concentrations after partial hepatectomy in rats.

The role of carnitine in the early phase of liver regeneration after partial hepatectomy has been a controversial issue. The purpose for this study was to evaluate the alterations of remnant liver carnitine and serum carnitine concentrations in partial-hepatectomized rats. Partial hepatectomy, with resection of the median and left lateral lobes (67%), was done in 40 male Wistar rats weighing about 200 g. Another group of 40 rats underwent a sham-operation for comparison. All the rats underwent fasting for 4 hours before surgical procedure and 2 hours after surgery. They were killed at 6, 24, 48 or 72h after the operation, ten rats for each time. The serum carnitine concentration, remnant liver carnitine content, serum free fatty acid (FFA), acetoacetate (AA) and beta-hydroxybutyrate (BB) concentrations were measured. It was found that: (1) the serum carnitine concentration increased in the early posthepatectomized stage; (2) the carnitine content in the remnant liver increased in the early posthepatectomized stage; and (3) the serum FFA and its metabolites markedly increased with a depressed ketone body ratio noted in the early post-hepatectomized stage. These results supported the theory that regenerating liver utilized free fatty acids as an immediate main substrate. The utilization then decreased. Because of a marked increase of the carnitine concentration in the remnant liver and serum, it is suggested that the carnitine contents were sufficient for liver regeneration. There must be other reasons for the decreased utilization of free fatty acids.

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