Procedia food science最新文献

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Aerobic Microbial Inactivation Kinetics of Shrimp Using a Fixed Minimal Ozone Discharge: A Fact or Fib During Iced Storage? 使用固定最小臭氧排放对虾的好氧微生物失活动力学:在冷冻储存期间是事实还是谎言?
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.084
Charles Odilichukwu R. Okpala , Gioacchino Bono , Fabio Falsone , Maria Valentina Cani , Danilo Scannella , Federico Di Maio
{"title":"Aerobic Microbial Inactivation Kinetics of Shrimp Using a Fixed Minimal Ozone Discharge: A Fact or Fib During Iced Storage?","authors":"Charles Odilichukwu R. Okpala ,&nbsp;Gioacchino Bono ,&nbsp;Fabio Falsone ,&nbsp;Maria Valentina Cani ,&nbsp;Danilo Scannella ,&nbsp;Federico Di Maio","doi":"10.1016/j.profoo.2016.02.084","DOIUrl":"10.1016/j.profoo.2016.02.084","url":null,"abstract":"<div><p>Among researchers worldwide, the combination of preservation methods aimed to achieve improved effects on microbial inactivation of seafood products is an area of research receiving increasing interest. Globally also, the demand for high quality minimally processed food products are on the increase. Ozone treatment, three decade – long declared ‘Generally Recognized As Safe’ and approved as food contact sanitizing agent has evolved up to recent times where it assumes the likes of domestic food-processing facilities manufactured with environment-friendly status ensuring consumer safety. On the other hand, the subject of inactivation kinetics of seafood microorganisms following ozone treatment is still under debate. Furthermore, kinetic models remain the economical and quick approach to predict the preservation parameters. Nevertheless, there is paucity of information regards aerobic microbial inactivation of crustacean product arising from fixed minimal ozone discharge. Is the phenomenon of aerobic microbial inactivation kinetics of shrimp product subject to a fixed minimal ozone discharge during iced storage a fact or fib? To answer this, the aerobic microbial inactivation kinetics of shrimp during iced storage of up to 11 days was inspected. The process conditions comprised of a fixed ozone concentration of 100<!--> <!-->mg/h minimally discharged at wash time of 1<!--> <!-->min as well as iced storage of up to 11 days. Minimal ozone treatment was applied either prior to or during iced storage situations. Aerobic microbial inactivation presented significant effects during iced storage (P&lt;0.05). Line of fit that could best describe the aerobic microbial inactivation kinetics showed adequacy only at the fourth order of storage time ‘<em>x</em>’ variable, which could only but account for between 75 - 96% of explained variance. Overall, aerobic microbial inactivation kinetics of shrimp using a fixed minimal ozone discharge appears quantitatively possible even though it decreases as iced storage progresses.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"7 ","pages":"Pages 47-52"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82180874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Silicon Rich Rice Hull Amended Soil Enhances Anthracnose Resistance in Tomato 富硅稻壳改良土壤提高番茄抗炭疽病能力
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.046
Koshala Somapala , Darshani Weerahewa , Sunil Thrikawala
{"title":"Silicon Rich Rice Hull Amended Soil Enhances Anthracnose Resistance in Tomato","authors":"Koshala Somapala ,&nbsp;Darshani Weerahewa ,&nbsp;Sunil Thrikawala","doi":"10.1016/j.profoo.2016.02.046","DOIUrl":"10.1016/j.profoo.2016.02.046","url":null,"abstract":"<div><p>Silicon (Si) has proven to be effective in controlling diseases in many crops and can be used as a substitute of fungicides. It has been shown that rice hull could be applied to the soil as a non-hazardous silicon rich source in crop production. This study was conducted to investigate the effect of half burnt rice hull (HBRH) as a silicon source in suppression of anthracnose in tomato and understanding underlying mechanisms of disease resistance. HBRH was made by burning raw rice hull at 250<!--> <sup>°</sup>C for 15<!--> <!-->minutes. Six different levels of HBRH: 0, 5, 10, 15, 20 and 25<!--> <!-->g per 1<!--> <!-->kg of soil were incorporated into soil along with Department of Agriculture (DOA) recommended levels of NPK fertilizers. The resistance against anthracnose disease was evaluated by measuring the lesion area of tomato fruits followed by the challenged inoculation with <em>Colletotrichum dematium</em> for 10 days after inoculation. The mechanism behind Si induced resistance was studied by measuring the fruit firmness and cuticle thickness (CT). A significant reduction (at <em>P</em> ≤ 0.05) of anthracnose disease (87 and 77%) was observed in inoculated fruits of 25 and 20<!--> <!-->g HBRH per 1<!--> <!-->kg of soil treatments. Onset of the disease was delayed by 4 and 3 days after inoculation in fruits obtained from 25 and 20<!--> <!-->g HBRH treated plants compared to the control. The increase of fruit firmness and CT were significant in 25 and 20<!--> <!-->g HBRH per 1<!--> <!-->kg of soil provided fruits. Consequently, these results suggest that HBRH is a Si rich source and 20<!--> <!-->g HBRH per 1<!--> <!-->kg soil would be sufficient in suppressing tomato anthracnose caused by <em>C</em>. <em>dematium</em> and the resistance might have triggered with silicon induced thicken cuticle and increased fruit firmness.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"6 ","pages":"Pages 190-193"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80794059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
International Conference of Sabaragamuwa University of Sri Lanka (ICSUSL) 2015 2015斯里兰卡萨巴拉加穆瓦大学国际学术会议
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.001
Chaminda Niroshan Walpita Dr., K.K.D.S. Ranaweera Prof., Iddya Karunasagar Prof., Partick Sorgeloos Prof.
{"title":"International Conference of Sabaragamuwa University of Sri Lanka (ICSUSL) 2015","authors":"Chaminda Niroshan Walpita Dr.,&nbsp;K.K.D.S. Ranaweera Prof.,&nbsp;Iddya Karunasagar Prof.,&nbsp;Partick Sorgeloos Prof.","doi":"10.1016/j.profoo.2016.02.001","DOIUrl":"10.1016/j.profoo.2016.02.001","url":null,"abstract":"","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"6 ","pages":"Pages 1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84586584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Challenges of Food Security – Need for Interdisciplinary Collaboration 粮食安全的挑战——跨学科合作的需要
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.005
Indrani Karunasagar , Iddya Karunasagar
{"title":"Challenges of Food Security – Need for Interdisciplinary Collaboration","authors":"Indrani Karunasagar ,&nbsp;Iddya Karunasagar","doi":"10.1016/j.profoo.2016.02.005","DOIUrl":"10.1016/j.profoo.2016.02.005","url":null,"abstract":"<div><p>A peep into the ways global systems have impacted food security and the scientific processes to enhance food productivity is being outlined .Challenges such as food safety, global environmental changes and malnutrition need a multidisciplinary approach to tackle the issues associated with them. Multidisciplinary research in diverse areas related to crop production, public health, biological, physical and mathematical sciences, predictive modeling, statistical approaches etc. is the need of the hour. The coming together of all stake holders involved in science, technology and social sciences is required to implement progressive approaches to maximize production for feeding the ever increasing population and involves studies related to storage, manufacture, retail, food supply and value chain, reducing waste by preventing post harvest losses, recycling, making food safe by eliminating food related illness due to biological and chemical hazards, adopt best measures of preservation of quality, micronutrient addition to food for enhancing nutrition etc. All of the above needs to be addressed for sustaining global food security.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"6 ","pages":"Pages 31-33"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76837400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
The Analysis of the Behaviour of Listeria Monocytogenes in Fresh Cheeses with Various Spices During Storage 不同香料鲜奶酪贮存过程中单核细胞增生李斯特菌的行为分析
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.092
Adriana Lobacz, Justyna Zulewska, Jaroslaw Kowalik
{"title":"The Analysis of the Behaviour of Listeria Monocytogenes in Fresh Cheeses with Various Spices During Storage","authors":"Adriana Lobacz,&nbsp;Justyna Zulewska,&nbsp;Jaroslaw Kowalik","doi":"10.1016/j.profoo.2016.02.092","DOIUrl":"10.1016/j.profoo.2016.02.092","url":null,"abstract":"<div><p>The aim of this work was to evaluate the growth possibilities of <em>Listeria monocytogens</em> in the artificially contaminated fresh cheese during storage. Three variants of acid homogenized commercial fresh cheeses: mixed herbs, red pepper and garlic-pepper were studied. The lowest value of the specific growth rate of <em>L. monocytogenes</em> in the fresh cheeses was reported for red pepper variant. The highest value of this parameter was determined for the fresh cheese with pepper-garlic. The use of spices in the production of fresh unripened cheeses influence the viability and growth of bacteria <em>L. monocytogenes</em> during storage at the temperature range 3-15<sup>o</sup>C.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"7 ","pages":"Pages 80-84"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85448142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Role of Underutilized Crop Value Chains in Rural Food and Income Security in Sri Lanka 未充分利用的作物价值链在斯里兰卡农村粮食和收入安全中的作用
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.049
Arosh Bandula , Champika Jayaweera , Achini De Silva , Patrick Oreiley , Asha Karunarathne , S.H.P. Malkanthi
{"title":"Role of Underutilized Crop Value Chains in Rural Food and Income Security in Sri Lanka","authors":"Arosh Bandula ,&nbsp;Champika Jayaweera ,&nbsp;Achini De Silva ,&nbsp;Patrick Oreiley ,&nbsp;Asha Karunarathne ,&nbsp;S.H.P. Malkanthi","doi":"10.1016/j.profoo.2016.02.049","DOIUrl":"10.1016/j.profoo.2016.02.049","url":null,"abstract":"<div><p>Agricultural value chain is fundamental to the survival of human society, the growth or maintenance of regional and national economies, and the wealth and welfare of individual producers. Few staple food crops holding the lion's loin of food and income security of the rural farming communities where the role of unrecognized and underutilized crops was invisible. Previous researches and many past applications were focused on individual elements of the value chain rather than the holistic view. Complex interacting drivers in production, processing, marketing, consumption, environment and the people were neglected and this limitation hiders the recognition of underutilized crops. Our paper takes a critical stock take of underutilized crop value chains in Sri Lanka and focused to identify its role in rural food and income security with special concern to the availability of those crops at respective village markets and at their own household premises. Study aimed to map the underutilized crop value chain and describe its upstream and downstream nodes, agents, functions, and products. For the purpose of this study, mixed methods approach was employed which focuses on research questions that call for real-life contextual understandings on value chain agents, multi-level perspectives, both vertical and horizontal linkages and cultural interpretations. We employed qualitative research exploring the meaning and understanding of constructs; utilizing multiple methods (rapid market chain analysis). Value chain architecture explains the structure, agents, and strength of the relationships. Downstream composed of two main nodes, farmers, wild collectors and traders or village level collectors link with the upstream. Primary processors, secondary processors, wholesalers, retailers, and consumers were the key upstream agents. Chain structure heavily depends on the type of crop. Downstream activities depends on location, water availability, season and market demand which decides the crop mix. Informal collecting centres were common and village level collectors link farmers and wild collectors with upstream. Upstream activities were more concern on value addition and creation and the agents were multifunctional. Food basket analysis highlighted that underutilized crops were more attracted to urban consumers than the rural. Meal analysis revealed that considerable amount of underutilized crops consumed at household level were own production or wild collection. Complex and indirect income generation process was unrecognized yet.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"6 ","pages":"Pages 267-270"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85640699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Estimation of the Risk Associated to Marketing of Swine Meat Contaminated with Salmonella spp., Employing the William T. Fine Method 用William T. Fine方法估计受沙门氏菌污染猪肉的销售风险
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.05.005
Nataly Ruiz Quiñones , Oscar Castro Aguilar , Martha Lily Ocampo Guerrero
{"title":"Estimation of the Risk Associated to Marketing of Swine Meat Contaminated with Salmonella spp., Employing the William T. Fine Method","authors":"Nataly Ruiz Quiñones ,&nbsp;Oscar Castro Aguilar ,&nbsp;Martha Lily Ocampo Guerrero","doi":"10.1016/j.profoo.2016.05.005","DOIUrl":"10.1016/j.profoo.2016.05.005","url":null,"abstract":"<div><p>Swine meat is a growing market in Colombia, reaching 3.1 million of carcasses sold in 2014. However, it is estimated that the proportion of hogs illegally marketed can be up to 50%, which exposes the population to foodborne pathogens such as Salmonella. The aim of this study was to estimate the risk associated to the marketing of swine meat contaminated with Salmonella, using the William T. Fine method in Tolima Department. The risk scenarios applied to four municipalities positive for Salmonella showed a medium and low level of risk, and an exposed population of 114,000 people, representing 8.7% of the Department's population. In the worst-case scenario, costs for hospitalization and compensation could represent more than US$ 145 billion for the biggest municipality. It can be concluded that the William T. Fine method is applicable for biological risk analysis focused on foodborne pathogen outbreaks.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"7 ","pages":"Pages 137-140"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.05.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85076129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Joint Application of Antimicrobial Agents on Microbial Flora Chilled Meat Cattle. Use of Mathematical Models 抗菌剂在冷鲜肉牛微生物菌群中的联合应用。数学模型的使用
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.087
María C. Álvarez, Irene Pena, María C. Villat, Pablo de la Sota, Gladys Laporte, Daniela Olivera, Miguel Noia, Fernanda Coll Cárdenas
{"title":"Joint Application of Antimicrobial Agents on Microbial Flora Chilled Meat Cattle. Use of Mathematical Models","authors":"María C. Álvarez,&nbsp;Irene Pena,&nbsp;María C. Villat,&nbsp;Pablo de la Sota,&nbsp;Gladys Laporte,&nbsp;Daniela Olivera,&nbsp;Miguel Noia,&nbsp;Fernanda Coll Cárdenas","doi":"10.1016/j.profoo.2016.02.087","DOIUrl":"10.1016/j.profoo.2016.02.087","url":null,"abstract":"<div><p>Objectives of this work were: i) to study the effect joint application of antimicrobial agents on microbial flora chilled meat cattle;ii) to model mathematically this microbial growth and iii) validate the models with own experimental results.</p><p>Samples were irradiated with UVC light for 5 minutes and then were added a solution of oregano oil and lactic acid (1: 1). Untreated samples were considered as control.</p><p>Gompertz model was appropriate to quantify microbial growth in all conditions studied. Meanwhile, <em>Pseudomonas sp</em> in treated samples showed no development, having to apply the linear regression model.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"7 ","pages":"Pages 63-66"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85108271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effect of Coconut Kernel Residues on Serum Lipid Concentrations of Rats 椰子仁渣对大鼠血脂浓度的影响
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.045
L.L.W.C. Yalegama , R. Sivakanesan , D.N. Karunarathna
{"title":"Effect of Coconut Kernel Residues on Serum Lipid Concentrations of Rats","authors":"L.L.W.C. Yalegama ,&nbsp;R. Sivakanesan ,&nbsp;D.N. Karunarathna","doi":"10.1016/j.profoo.2016.02.045","DOIUrl":"10.1016/j.profoo.2016.02.045","url":null,"abstract":"<div><p>The coconut kernel residues are underutilized by products in coconut milk and coconut oil industries. The residue from coconut milk (MR) and virgin coconut milk (VOR) can be used effectively for human consumption. Therefore, studying its nutritional effects on serum lipid profile is very important to promote for human consumption. The objective of this study was to determine the nutritional effect of VOR and MR incorporated diets on serum lipid profile of rats. The experimental diet was prepared by incorporating 10% or 20% VOR or 10% or 20% MR. The control diet did not include VOR or MR. The blood samples were drawn at base level and thereafter at 30 days intervals. The initial concentrations were compared with the concentrations at each days using unpaired t test for testing significant differences (p=0.05). The increase of serum TC concentration of rats fed with 10 and 20% MR and 20% VOR was19%, 17% and 16% respectively while the increase shown by control group and 10% VOR group was 20% and 22% respectively at 120 days although there is no significant change. Significant increase in serum HDL-C was shown by rats fed with 20% VOR in 30 days. Triacylglyceride, TAG, concentrations of rats fed with 20% VOR significantly decreased significantly by 22% in 120 days while the control group increased significantly by 31%. However, 10% VOR and 20% MR fed group showed insignificant decrease in serum TAG. This study reveals that VOR and MR can potentially decrease the serum level of TC and TAGs in human.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"6 ","pages":"Pages 186-189"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88569927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Genome Sequencing of Seafood-borne Vibrio Parahaemolyticus VP49 Reveals the Presence of Novel Virulence Attributes 海产副溶血性弧菌VP49基因组测序显示存在新的毒力属性
Procedia food science Pub Date : 2016-01-01 DOI: 10.1016/j.profoo.2016.02.066
B.K. Kumar, V.K. Deekshit, P. Rai, I. Karunasagar
{"title":"Genome Sequencing of Seafood-borne Vibrio Parahaemolyticus VP49 Reveals the Presence of Novel Virulence Attributes","authors":"B.K. Kumar,&nbsp;V.K. Deekshit,&nbsp;P. Rai,&nbsp;I. Karunasagar","doi":"10.1016/j.profoo.2016.02.066","DOIUrl":"10.1016/j.profoo.2016.02.066","url":null,"abstract":"<div><p><em>Vibrio parahaemolyticus</em> is a Gram-negative, halophilic bacterium autochthonous to the marine environments and responsible for seafood-borne gastroenteritis. Though the hemolysins TDH and/or TRH are classical virulence factors, several other potential virulence factors may contribute to their pathogenicity. In this study, <em>V. parahaemolyticus</em> was isolated from the seafood harvested along southwest coast of India and confirmed by standard biochemical and molecular methods. Genome sequencing of <em>V. parahaemolyticus</em> revealed the presence of T3SS2 operon in an approximately 44<!--> <!-->kb region in close proximity to the hemolysin gene <em>trh</em>. The annotation of T3SS2 operon revealed the presence of genes encoding apparatus proteins <em>VscC2/R2/S2/T2/U2/N2, VcrD2</em>, an ATPase <em>VscN2</em>, translocons<em>VopB2/D2</em>, and effectors <em>VopA</em>/C/<em>L</em>. To the best of knowledge, this is the first report on sequencing and characterization of a T3SS gene cluster in seafood isolate of <em>V. parahaemolyticus</em> and this information will be of assistance in future studies to determine the different virulence attributes as well as mechanisms that enhance environmental or host fitness of <em>V. parahaemolyticus.</em> The presence of such virulence attributes in <em>V. parahaemolyticus</em> isolated from seafood suggests the potential of these isolates to cause infection in humans upon ingestion of contaminated seafood and questions the safety of seafood to consumers.</p></div>","PeriodicalId":20478,"journal":{"name":"Procedia food science","volume":"6 ","pages":"Pages 331-333"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.profoo.2016.02.066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82143061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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