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Procedure for isolation of the plastic degrading piezophilic bacteria from deep-sea environments 从深海环境中分离塑料降解嗜压细菌的程序
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.9.25
Takayoshi Sekiguchi, Takako Sato, M. Enoki, H. Kanehiro, C. Kato
{"title":"Procedure for isolation of the plastic degrading piezophilic bacteria from deep-sea environments","authors":"Takayoshi Sekiguchi, Takako Sato, M. Enoki, H. Kanehiro, C. Kato","doi":"10.3118/JJSE.9.25","DOIUrl":"https://doi.org/10.3118/JJSE.9.25","url":null,"abstract":"A procedure for the isolation of aliphatic polyester-degrading bacteria from deep-sea environments was developed using three step high-pressure cultivation. First, the sediments collected from the deep-sea floor, the Kurile and Japan Trenches at a depth of 5000-7000 m, were cultivated with poly e-caprolactone (PCL) films under low-temperature (4°C) and high-pressure (50MPa) conditions. Secondly, high-pressure continuous cultivation using the deep-sea baro-piezophile and thermophile isolation and cultivation (DEEPBATH) system was performed three times to accumulate piezophilic bacteria. Finally, piezophilic PCL degrading bacteria were isolated using the pressure bag method involving media with PCL granules. Using this procedure, we succeeded in isolating several piezophilic aliphatic polyester-degrading bacteria from the sediment samples of deep-trenches.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115832879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Cultivation of uncultured microbes and microbial network analysis 未培养微生物的培养及微生物网络分析
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.74
Y. Kamagata
{"title":"Cultivation of uncultured microbes and microbial network analysis","authors":"Y. Kamagata","doi":"10.3118/JJSE.6.74","DOIUrl":"https://doi.org/10.3118/JJSE.6.74","url":null,"abstract":"(1) はじめに 難培養性微生物という言葉が微生物学の世界で広 く使われるようになったのはここ 10 数年のことで ある。しかし微生物学に携わる研究者はそれ以前に 培養できない微生物が数多く存在していることを何 も知らなかった訳ではない。どんな環境試料を顕微 鏡で観察して見ても、実に多様な形態や運動性を有 する微生物がそこに存在していることがわかる。し かし、これらの試料を寒天培地上に接種して出現す るコロニーを、一つ一つ、つぶさに観察してみたと しても限られた姿の微生物しか生育しておらず、も との試料に存在していたはずの”多様な微生物”が寒 天培地の上では忽然と姿を消してしまっていること に誰しも気づいていた。少なくとも 20 年前までの微 生物学であればここで話が終わってしまっていただ ろう。実際、培養できない(できなかった)微生物 とは一体何なのかを想像するすべもなかったからで ある。 しかし 1990 年代前半から微生物学は革命的な変 貌をとげた。20 世紀の最後から今日までの 10 数年 間は端的にいえば微生物を培養することなくその存 在や機能・性質の一端を明らかにすることができる ようになった時代である。その結果、これまでに知 られている微生物の種類は実際に地球上に存在する であろう微生物種のほんの一握りであること、そし て我々が研究のために扱ってきた微生物はもっぱら たやすく培養できる“特別”な微生物だったことが明 らかになった。とりわけ 16S rRNA 遺伝子配列の情 報蓄積がこれまでまったく知られていなかった膨大 な微生物群の存在を初めて我々に知らしめたのであ る。","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115870458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Halomonas sp. strain DT-W, a halophile from the 11,000 m-depth of the Mariana Trench 盐单胞菌属菌株DT-W，一种来自马里亚纳海沟11000米深处的嗜盐菌
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.5.27
H. Tamegai, T. Furukawa, J. Nitta, Sayaka Chikuma, M. Miyazaki, Y. Nogi, Shizuka Arakawa, C. Kato, K. Horikoshi
{"title":"Halomonas sp. strain DT-W, a halophile from the 11,000 m-depth of the Mariana Trench","authors":"H. Tamegai, T. Furukawa, J. Nitta, Sayaka Chikuma, M. Miyazaki, Y. Nogi, Shizuka Arakawa, C. Kato, K. Horikoshi","doi":"10.3118/JJSE.5.27","DOIUrl":"https://doi.org/10.3118/JJSE.5.27","url":null,"abstract":"Moderately halophilic strain DT-W was isolated from the mud of the Mariana Trench. Cells of the organism were rod-shaped (1.5-2 m x 0.5-0.8 µm) with some flagella extruding from the cells. Growth occurred in an NaCl concentration of about 0.1-15% (optimal: 3-5%), at pH of 6-9, and at temperatures ranging from 4-51 °C (optimal: 30-37 °C). The results of 16S rDNA analysis and DNA-DNA hybridization analyses showed that DT-W was closely related to Halomonas aquamarina, Halomonas axialensis and Halomonas meridiana. Furthermore, additional physiological properties and the cytochrome contents of DT-W were also analyzed.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131014490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Search for genes that confer acid tolerance to plants 寻找赋予植物耐酸能力的基因
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.7.2.20
T. Aoki, M. Shimamura, Y. Sawada, R. Imaizumi
{"title":"Search for genes that confer acid tolerance to plants","authors":"T. Aoki, M. Shimamura, Y. Sawada, R. Imaizumi","doi":"10.3118/JJSE.7.2.20","DOIUrl":"https://doi.org/10.3118/JJSE.7.2.20","url":null,"abstract":"To remediate the worldwide-spreading acid sulphate soil area and utilize it for human beings, acid tolerant leguminous plants are expected to be useful as crops and pioneer plants. The purpose of this study is to produce symbiotic nitrogen fixing systems tolerant to acid sulfate soils, through the identification of the genes that confer acid tolerance on plants and the isolation of acid-tolerant rhizobia. We identified acid tolerant genes by two approaches. One approach is functional screening of a soybean cDNA library and their characterization in planta. E. coli cells were transformed with a cDNA library of soybean and cultured on a LB medium adjusted to pH 4 or pH 5. Selected six tolerant clones were introduced into Arabidopsis thaliana using a plant expression vector. The overexpressors grew better than the wild type under the acid and aluminium stresses. The other one is molecular genetic analysis of the candidate genes identified by previous studies: a gene for a key enzyme of sulfur assimilation, cysteine synthase. The candidate gene was ectopically overexpressed in A. thaliana under the control of the cauliflower mosaic virus 35S promoter. All the genes tested were shown to confer acid tolerance on the model plants.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131273655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification of NADPH dehydrogenase from a hyperthemophile, Pyrobaculum islandicum 从嗜热菌岛火杆菌中纯化NADPH脱氢酶
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.8.31
M. Tanigawa, S. Seki, M. Mohri, A. Harigae, Y. Nagata
{"title":"Purification of NADPH dehydrogenase from a hyperthemophile, Pyrobaculum islandicum","authors":"M. Tanigawa, S. Seki, M. Mohri, A. Harigae, Y. Nagata","doi":"10.3118/JJSE.8.31","DOIUrl":"https://doi.org/10.3118/JJSE.8.31","url":null,"abstract":"A NADPH dehydrogenase was purified from the hyperthermophilic archaeon, Pyrobaculum islandicum. The membrane protein was solubilized by treatment with 1% Tween 20, and purified 258-fold from the cell-free extract to homogeneity as judged by SDS-PAGE analysis. The molecular mass of the enzyme was revealed to be 41 kDa both by SDS-PAGE and gel filtration analyses. The optimal pH and temperature were 7.0 and above 80°C, respectively. The Km and Vmax values were 0.69 mM and 0.25 nmol/min, respectively. It was suggested that NADPH is an electron donor in the P. islandicum electron transfer system.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117160288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Looking back on my research life. 回顾我的研究生涯。
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.8.15
R. Iizuka
{"title":"Looking back on my research life.","authors":"R. Iizuka","doi":"10.3118/JJSE.8.15","DOIUrl":"https://doi.org/10.3118/JJSE.8.15","url":null,"abstract":"75 - ， Abstract Having reached my retirement age ， I retired from Kagawa Prefectural University of Health Sciences on March 31, 2014 ． The main area of my research interest for 40 years was endocrinology ． In this article ， I look back on my encounters with people who kindly supported my research ． In addition ， I summarize the four main themes of my recent research ：( 1 ） Central mechanisms of appetite regulation ；( 2 ） Clinical research related to obesity ；( 3 ） Role of neuropeptides related to stress ；( 4 ） Methods to evaluate stress and their clinical application ． I sincerely thank Kagawa Prefectural University of Health Sciences for giving me the opportunity to contribute to this journal.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133963746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulation and environmental response of aromatic degradation gene expression in a PCB degrading bacterium. 多氯联苯降解菌芳香族降解基因表达的调控及环境响应。
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.63
M. Fukuda
{"title":"Regulation and environmental response of aromatic degradation gene expression in a PCB degrading bacterium.","authors":"M. Fukuda","doi":"10.3118/JJSE.6.63","DOIUrl":"https://doi.org/10.3118/JJSE.6.63","url":null,"abstract":"","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132526216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Involvement of YkoN in production of a new phospholipid in Bacillus subtilis Marburg YkoN参与马尔堡枯草芽孢杆菌一种新磷脂的生产
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.38
T. Matsuura, T. Terayama, R. Usami, H. Yoshida, H. Hara, K. Matsumoto
{"title":"Involvement of YkoN in production of a new phospholipid in Bacillus subtilis Marburg","authors":"T. Matsuura, T. Terayama, R. Usami, H. Yoshida, H. Hara, K. Matsumoto","doi":"10.3118/JJSE.6.38","DOIUrl":"https://doi.org/10.3118/JJSE.6.38","url":null,"abstract":"To understand the physiological role of the ykoN gene of Bacillus subtilis, the gene was expressed from the IPTG-inducible spac promoter in B. subtilis cells. When lipid composition of the cells induced for ykoN expression was examined, a new spot of phospholipid was found on the thin layer chromatogram. Induction of ykoN in Escherichia coli cells produced a similar new spot. The new spot was produced in mutant E. coli cells lacking cardiolipin or phosphatidylethanolamine, but not in the cells lacking phosphatidylglycerol. The result suggests an involvement of phosphatidylglycerol in production of the new spot. Examination of the lipid composition during the stages of B. subtilis growth revealed that the new phospholipid was produced four hours after cessation of logarithmic growth, consistent with an increase in the promoter activity of ykoN in the late stage in sporulation. YkoN has the pentapeptide lipase/esterase motif (Gly-X-Ser-X-Gly), and shows a lipolytic activity (Matsuura et al., in press). The mutant YkoN (S205A) in which the central serine residue of the motif is replaced with alanine produced the new phospholipid in the amount as small as only 1/10 of the wild type, suggesting that the lipolytic activity of YkoN is involved in the new phospholipid production.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115698368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of organic solvents, temperature and EDTA on activity and stability of halophilic .ALPHA.-amylase from Haloarcula sp. S-1 有机溶剂、温度和EDTA对亲盐α活性和稳定性的影响。-淀粉酶来自Haloarcula sp. S-1
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.4.56
Tadamasa Fukushima, A. Echigo, K. Fukuda, R. Usami
{"title":"Effect of organic solvents, temperature and EDTA on activity and stability of halophilic .ALPHA.-amylase from Haloarcula sp. S-1","authors":"Tadamasa Fukushima, A. Echigo, K. Fukuda, R. Usami","doi":"10.3118/JJSE.4.56","DOIUrl":"https://doi.org/10.3118/JJSE.4.56","url":null,"abstract":"More than 80% of residual halophilic α-amylase (crude and purified) activity from strain S-1 was observed in the presence of various organic solvents. More than 78% remaining activity was detected after incubation for 120 hr in the presence of styrene, toluene, benzene or chloroform. Enzyme activity and stability with chloroform were higher than that without chloroform at the low or high temperature, and enzyme activity was higher in the presence of chloroform than one without chloroform when EDTA (5-15 mM) was added.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123827480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study on nitrogen cycling by using stable isotope techniques 利用稳定同位素技术研究氮循环
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.52
K. Koba
{"title":"Study on nitrogen cycling by using stable isotope techniques","authors":"K. Koba","doi":"10.3118/JJSE.6.52","DOIUrl":"https://doi.org/10.3118/JJSE.6.52","url":null,"abstract":"","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122230062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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