Journal of Japanese Society for Extremophiles最新文献_第4页

Gene expression and characterization of a novel GH family 18 chitinase from extremely halophilic archaeon Halobacterium salinarum NRC-1 极端嗜盐古细菌盐芽菌NRC-1 GH家族18几丁质酶的基因表达与特性研究

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.9.19

R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, Y. Zhang, T. Takashina, T. Fukui, Satoshi Nakamura

引用次数: 9

Nucleoside diphosphate kinase of halobacteria 盐细菌的核苷二磷酸激酶

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.3.1_18

Toru Mizuki, M. Kamekura, M. Ishibashi, R. Usami, Yasuhiko Yoshida, M. Tokunaga, K. Horikoshi

引用次数: 4

Role of an N-terminal domain found in the ferredoxin from extremely halophilic archaeon Haloarcula japonica 极端嗜盐古菌日本盐环菌铁氧还蛋白n端结构域的作用

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.4.14

N. Hirota, T. Matsuo, A. Ikeda, R. Yatsunami, T. Fukui, Satoshi Nakamura

{"title":"Role of an N-terminal domain found in the ferredoxin from extremely halophilic archaeon Haloarcula japonica","authors":"N. Hirota, T. Matsuo, A. Ikeda, R. Yatsunami, T. Fukui, Satoshi Nakamura","doi":"10.3118/JJSE.4.14","DOIUrl":"https://doi.org/10.3118/JJSE.4.14","url":null,"abstract":"The ferredoxin (Fd) from Haloarcula japonica possesses a plant-type [2Fe-2S] cluster and is stable at high salt concentrations. Ha. japonica Fd (HjFd) includes an N-terminal additional domain rich in acidic amino acids, as well as a common core domain that contains the Fe-S cluster. The N-terminally HAT-tagged intact HjFd (HAT/HjFd) and spinach/Ha. japonica chimeric Fd (HAT/Sp/HjFd) were prepared and characterized. Escherichia coli-produced HAT/Sp/HjFd and Ha. japonica-produced HAT/HjFd were produced as holoproteins. On the other hand, E. coli-produced HAT/HjFd did not incorporate the Fe-S cluster. These results suggested that the N-terminal domain of HjFd contributed to the polypeptide folding and successive Fe-S cluster incorporation under high salt conditions. Both Ha. japonica-produced HAT/HjFd and E. coli-produced HAT/Sp/HjFd were stable at high salt concentrations (≥1.5 M NaCl), although a reduction in stability was observed at lower concentrations. Lack of the N-terminal domain did not affect the stability of HjFd, indicating that the core domain mainly contributed to the stability of HjFd at high salt concentrations. Solubility of E. coli-produced HAT/Sp/HjFd under high salt conditions was significantly lower than that of Ha. japonica-produced HAT/HjFd. It was revealed that substitution of the N-terminal domain of HjFd to that of spinach Fd injured the solubility of HjFd. Thus, it was concluded that the N-terminal domain of HjFd should perform the essential functions for halophilic adaptation from the folding process through the folded state.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125150143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Ecological lilationships among the viruses and thraustochytrids 病毒与喉癣病的生态关系

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.68

Y. Takao

{"title":"Ecological lilationships among the viruses and thraustochytrids","authors":"Y. Takao","doi":"10.3118/JJSE.6.68","DOIUrl":"https://doi.org/10.3118/JJSE.6.68","url":null,"abstract":"ラビリンチュラ類は海洋に生息する直径 5 20μm の無色の原生生物であり、沿岸域において腐食性または寄生性生物として様々な試料(海水, 底泥, 藻類、植物、軟体動物の表面など)から普遍的に分離される真核生物である。一般に、陸上生態系においては菌類と細菌類が主要な分解者と考えられているのに対し、海洋生態系の分解者としては細菌類のみが重要視されてきた。しかしながら近年、沿岸生態系におけるラビリンチュラ類の巨大なバイオマスや不飽和脂肪酸蓄積能が解明されるとともに、同生物群の分解者としての重要性が注目されるようになった。一方、海洋には夥しい量のウイルス(10-10 / ml) が存在しており、ウイルス感染が宿主生物の現存量変動に大きく影響していることが明らかとなってきた。ウイルス感染は、宿主生物を死滅・分解に導くことで有光層のエネルギーサイクルからの炭素・窒素およびその他の栄養塩の漏出を防いでいるという点で、浅海生態系の維持に大きく貢献していると考えられている。こうした背景の下、海洋においてウイルスが果たす生態学的役割の重要性に関する認識が着実に高まりつつある。ごく近年、筆者らはラビリンチュラ類を宿主とするウイルスの単離に世界で初めて成功し、それらの性状を精査した。本稿では、ラビリンチュラ類とウイルスについて今日までに得られている知見を概説するとともに、ラビリンチュラ類感染性ウイルスの研究から新たに得られた宿主ラビリンチュラ類に関する生態学的知見について紹介する。 2. ラビリンチュラ類とは","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122225348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a Novel Esterase YkoN from Bacillus subtilis Marburg 马尔堡枯草芽孢杆菌新型酯酶YkoN的鉴定

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.32

T. Matsuura, R. Usami, H. Hara, K. Matsumoto

引用次数: 0

Cold-seep microbial communities are more abundant at deeper depths in the Japan Trench land slope. 冷渗微生物群落在日本海沟陆坡较深的地方更为丰富。

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.4.50

Shizuka Arakawa, M. Mori, L. Li, Y. Nogi, Takako Sato, Yasuhiko Yoshida, R. Usami, C. Kato

引用次数: 9