Journal of Japanese Society for Extremophiles最新文献

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Occurrence of Extreme Thermophiles, Thermaerobacter spp., in Sewage Sludge 极端嗜热菌热杆菌在污水污泥中的出现
Journal of Japanese Society for Extremophiles Pub Date : 2010-12-01 DOI: 10.3118/JJSE.9.67
Kota Shiotsuka, A. Tanaka, K. Sakai
{"title":"Occurrence of Extreme Thermophiles, Thermaerobacter spp., in Sewage Sludge","authors":"Kota Shiotsuka, A. Tanaka, K. Sakai","doi":"10.3118/JJSE.9.67","DOIUrl":"https://doi.org/10.3118/JJSE.9.67","url":null,"abstract":"We isolated an extreme thermophilic bacterium strain SS, which grew between 55 and 82°C (optimum 70°C), from sewage sludge in Kagoshima City, Japan. Morphological and physiological properties, as well as 16S rRNA gene sequence indicated that strain SS belongs to the genus Thermaerobacter. To confirm the distribution of Thermaerobacter spp. in sewage sludge, genus-specific detection by nested PCR of 16S rRNA gene was performed. As a result, the existence of Thermaerobacter spp. in six sewage sludge samples including composted sludge was demonstrated by sequencing of the nested PCR products. However, enrichment cultures with sewage sludge were needed to detect the target products, indicating that population of Thermaerobacter spp. may be significantly low. As far as we know, this is the first report on occurrence of extreme thermophilic bacteria in a municipal wastewater treatment process under mesophilic condition.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"166 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123295159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
「好鹽菌」、「嗜?菌」から「好嗜塩菌」へ 从“嗜盐菌”到“嗜盐菌”
Journal of Japanese Society for Extremophiles Pub Date : 2010-12-01 DOI: 10.3118/JJSE.9.57
正博 亀倉
{"title":"「好鹽菌」、「嗜?菌」から「好嗜塩菌」へ","authors":"正博 亀倉","doi":"10.3118/JJSE.9.57","DOIUrl":"https://doi.org/10.3118/JJSE.9.57","url":null,"abstract":"","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115587277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of a Haloarchaeal GH Family 18 Chitinase with Additional Acidic Amino Acids on Its Protein Surface 盐古菌GH家族18几丁质酶蛋白表面附加酸性氨基酸的特性研究
Journal of Japanese Society for Extremophiles Pub Date : 2010-12-01 DOI: 10.3118/JJSE.9.72
Y. Zhang, R. An, R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, T. Fukui, Satoshi Nakamura
{"title":"Characterization of a Haloarchaeal GH Family 18 Chitinase with Additional Acidic Amino Acids on Its Protein Surface","authors":"Y. Zhang, R. An, R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, T. Fukui, Satoshi Nakamura","doi":"10.3118/JJSE.9.72","DOIUrl":"https://doi.org/10.3118/JJSE.9.72","url":null,"abstract":"A chitinase (ChiN1) from Halobacterium salinarum NRC-1 belongs to the glycoside hydrolase family 18. According to the three-dimensional structure model of ChiN1, Asn239 and Gln242 on a small helix between Phe237 and Arg247 were targeted for mutagenesis. Mutants N239D (Asn239 was replaced by Asp) and Q242E (Gln242 was replaced by Glu) were expressed in Haloarcula japonica and then characterized. Characterization of the mutants revealed that halotolerancy could be improved by introducing an acidic amino acid on the surface of ChiN1.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130114561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A tertiary-branched tetra-amine, N4-aminopropylspermidine is a major cellular polyamine in an anaerobic thermophile, Caldisericum exile belonging to a new bacterial phylum, Caldiserica 三支四胺n4 -氨基丙基亚精胺是一种厌氧性嗜热菌Caldiserica的主要细胞多胺,属于一个新的细菌门Caldiserica
Journal of Japanese Society for Extremophiles Pub Date : 2010-12-01 DOI: 10.3118/JJSE.9.75
K. Hamana, H. Hayashi, M. Niitsu
{"title":"A tertiary-branched tetra-amine, N4-aminopropylspermidine is a major cellular polyamine in an anaerobic thermophile, Caldisericum exile belonging to a new bacterial phylum, Caldiserica","authors":"K. Hamana, H. Hayashi, M. Niitsu","doi":"10.3118/JJSE.9.75","DOIUrl":"https://doi.org/10.3118/JJSE.9.75","url":null,"abstract":"Acid-extractable cellular polyamines of thermophilic Caldisericum exile belonging to a new bacterial phylum, Caldiserica were analyzed by HPLC and GC. The coexistence of an unusual tertiary brancehed tetra-amine, N4-aminopropylspermidine with spermine, a linear tetra-amine, as the major polyamines in addition to putrescine and spermidine, is first reported in the moderate thermophile isolated from a terrestrial hot spring in Japan. Linear and branched penta-amines were not detected. The novel cellular polyamine profile found in the moderate thermophile has never been observed within various bacteria and archaea previously analyzed.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124039958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Dissimilatory Fe (III) reduction by cytochrome c-552 in a thermophilic, obligately chemolithoautotrophic bacterium, Hydrogenobacter thermophilus TK-6 嗜热嗜热氢杆菌TK-6中细胞色素c-552对Fe (III)的异化还原
Journal of Japanese Society for Extremophiles Pub Date : 2010-12-01 DOI: 10.3118/JJSE.9.61
N. Yoshida, N. Ohmura, N. Matsumoto, K. Sasaki, H. Saiki, H. Arai, M. Ishii, Y. Igarashi
{"title":"Dissimilatory Fe (III) reduction by cytochrome c-552 in a thermophilic, obligately chemolithoautotrophic bacterium, Hydrogenobacter thermophilus TK-6","authors":"N. Yoshida, N. Ohmura, N. Matsumoto, K. Sasaki, H. Saiki, H. Arai, M. Ishii, Y. Igarashi","doi":"10.3118/JJSE.9.61","DOIUrl":"https://doi.org/10.3118/JJSE.9.61","url":null,"abstract":"A thermophilic, obligately chemolithoauto- trophic H2-oxidizing bacterium, Hydrogenobacter thermophilus TK-6 can utilize O2 or NO3- as an electron acceptor. Here we show that this bacterium can grow autotrophically by the reduction of Fe(III)-EDTA or Fe(III)-DTPA (diethylene-triamine-pentaacetic acid) as an electron acceptor, through oxidation of H2 as an electron donor. A 7.6 kDa c-type cytochrome was purified from soluble fraction as the iron reducing protein and the protein was identified as cytochrome c-552. A rate constant for the reduction of Fe(III)-EDTA by the cytochrome was kinetically determined to be 1.73 x 107 s-1 by stopped-flow spectrophotometry, which is more than 20-fold higher than the constants of other iron reductases. The redox potential was measured as E = +0.27 V (vs NHE [Normal Hydrogen Electrode]), which is high enough to reduce Fe(III)-EDTA. These results suggest that cytochrome c-552 works as a terminal electron donor for Fe(III) in the periplasmic space.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124017843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The tardigrade Ramazzottius varieornatus as a model of extremotolerant animals 作为极端耐受性动物模型的缓步动物
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.7.2.25
D. Horikawa
{"title":"The tardigrade Ramazzottius varieornatus as a model of extremotolerant animals","authors":"D. Horikawa","doi":"10.3118/JJSE.7.2.25","DOIUrl":"https://doi.org/10.3118/JJSE.7.2.25","url":null,"abstract":"I report the successful culture of the tardigrade Ramazzottius varieornatus by supplying the green alga Chlorella vulgaris as a food. A standard strain named YOKOZUNA-1 has been isolated from single egg of R. varieornatus. The reared animals have anhydrobiotic ability throughout their life cycle in eggs, juveniles, and adults. Body water content of adult R. varieornatus drops from 78.6% wt./wt. in hydrated state to 2.5% wt./wt. in anhydrobiotic state. Reared adults, while in an anhydrobiotic state, are tolerant of exposure to 100°C, 1 GPa, 99.8% acetonitrile, and 5000 Gy of 4He ions. Furthermore, hydrated active adults survive freezing at -2 to -20°C at a cooling rate of 1°C/min. Based on useful culture systems and extraordinary tolerance to extreme environmental conditions of R. varieornatus, this species is expected to be a suitable model for extremophile research focusing on multicellular organisms.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131287847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Phylogenetic analysis of pectin degrading yeasts from deep-sea environments 深海环境中果胶降解酵母的系统发育分析
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.5.21
H. Minegishi, Takeshi Miura, Yasuhiko Yoshida, R. Usami, F. Abe
{"title":"Phylogenetic analysis of pectin degrading yeasts from deep-sea environments","authors":"H. Minegishi, Takeshi Miura, Yasuhiko Yoshida, R. Usami, F. Abe","doi":"10.3118/JJSE.5.21","DOIUrl":"https://doi.org/10.3118/JJSE.5.21","url":null,"abstract":"Deep-sea yeast strains were isolated from mud samples collected in Sagami Bay (1,100-1,400 m) and the Japan Trench (4,500-6,500 m). All of the 46 yeast isolates were capable of growth at 24°C and atmospheric pressure, suggesting that these strains might originate neritic regions or land but survived in deep sea. Based on sequencing of 26S rRNA gene, 15 strains were classified into basidiomycetous yeasts including Cryptococcus liquefaciens, Kondoa aeria, Rhodosporidium diobovatum, Rhodosporidium sphaerocarpum, Rhodotorula mucilaginosa, Rhodotorula dairenensis and Rhodotorula slooffiae, and 8 strains were classified into ascomycetous yeasts including Aureobasidium pullulans, Candida zeylanoides, Kluyveromyces nonfermentans, Metschnikowia bicuspidate and Williopsis saturnus. Screening of the 46 isolates appeared to yield a high frequency of polygalacturonase (PGase) producers capable of degrading pectin. We suggest that deep-sea yeasts are new sources of PGase producers.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"240 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114337002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Polyamine analysis of methanogens, thermophiles and extreme halophiles belonging to the domain Archaea 古菌领域产甲烷菌、嗜热菌和极端嗜盐菌的多胺分析
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.25
K. Hamana, Ryuichi Hosoya, T. Itoh
{"title":"Polyamine analysis of methanogens, thermophiles and extreme halophiles belonging to the domain Archaea","authors":"K. Hamana, Ryuichi Hosoya, T. Itoh","doi":"10.3118/JJSE.6.25","DOIUrl":"https://doi.org/10.3118/JJSE.6.25","url":null,"abstract":"Cellular polyamines of 61 archaeal newly published strains were analyzed by HPLC to evaluate correlation of polyamine profiles to their extremophily and chemotaxonomy. A quaternary branched penta-amine, N4-bis( aminopropyl )spermidine, was found in hyperthermophilic Methanocaldococcus and Methanotorris belonging to the family Methanocaldococcaceae but not in the family Methanococcaceae nor any other methanogenic archaeal orders. Hyperthermophilic / extremely thermophilic Palaeococcus, Thermococcus and Pyrococcus of the order Thermococcales ubiquitously contained the branched penta-amine. Mesophilic, extreme halophiles including halo-alkaliphiles, belonging to the order Halobacteriales, contained a trace of spermidine, spermine and agmatine. In the phylum Crenarchaeota, extremely thermophilic Caldisphaera of the provisional order 'Caldisphaerales' contained norspermidine, spermidine and agmatine, and hyperthermophilic Pyrobaculum belonging to the order Thermoproteales contained norspermidine, spermidine, norspermine and spermine, but not any penta-amines. Within the order Desulfurococcales, Desulfurococcus and Ignisphaera contained spermidine, norspermidine, homospermidine, norspermine and spermine, whereas Aeropyrum contained caldopentamine and thermopentamine in addition to these triamines and tetra-amines.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125679001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Expression of Bacterial Genome under Soil Environment 土壤环境下细菌基因组的表达
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.59
M. Tuda
{"title":"Expression of Bacterial Genome under Soil Environment","authors":"M. Tuda","doi":"10.3118/JJSE.6.59","DOIUrl":"https://doi.org/10.3118/JJSE.6.59","url":null,"abstract":"様々な自然生態系から多種多様な生物現象を示す 微生物株が数多く分離され、先人たちは実験室で純 粋培養したこれら微生物をいろいろな角度から検討 することで、個々の生物機能の分子メカニズム、そ して、システムとしての細胞や同一株集団における 当該生物機能の発現様式の詳細を明らかにしてきた。 その一方で、物理的、化学的並びに生物学的に複雑 な環境要因の大規模な変動に曝される複合生物系の 自然生態系で、研究対象としてきた環境微生物が実 験室で純粋培養したときと同じような様式で生物機 能を発揮しているのかという、シンプルだが最も重 要な問題は手がつけられないでいた。この類の包括 的な研究は、微生物の持つ生物機能を人間が自然環 境で利用するバイオレメディエーションなどをはじ めとする応用分野において、とりわけ重要である。 そして、生物機能を直接支配する個々の特定遺伝子 の生態系での量的変動や発現などの記載が行われて きた結果、生態系での遺伝子発現は実験室系の単独 培養時の発現とは異なっていると提唱されるように なった。ただ、この提唱には曖昧なところがたいへ ん多く、この原因として、How とか Why という観 点での研究を展開するための手法が不備であったこ とがあげられる。一方、動植物病原細菌を扱う研究 者は 1990 年代より、感染モデル生物を「培養装置」 と見立て、この環境で菌の生存・増殖に必要な遺伝 子や特異的に発現する遺伝子をゲノムワイドに探 索・解析することで、病原細菌の「培養装置」での 「真の」生きざまを明らかにし、病原性発揮に直接 的並びに間接的に必要な遺伝子群の総合的理解を深 めてきた(1, 2)。このような方向性を持った研究は、 微生物ゲノムの全塩基配列決定が容易になるにつれ、 さらに進展している。他方、環境細菌においては、 土壌などの自然環境を「培養装置」と見立て、本環 境で生存・増殖に必要な遺伝子や特異的に発現する 遺伝子を探索・解析する研究がなかったが、この数 年の間に自然環境での微生物の特定遺伝子やゲノム 全体の発現を解析する手法が急速に確立されてきた (3)。本稿では、土壌環境で実際に機能している遺伝 子をどのように検索・解析するかについて、我々の 最近の研究例(4)を簡単に紹介する。","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121622957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Distribution of long linear and branched polyamines in the thermophiles belonging to the domain Bacteria 属细菌域的嗜热菌中长线性和支化多胺的分布
Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.7.1.10
K. Hamana, Ryuichi Hosoya, A. Yokota, M. Niitsu, H. Hayashi, T. Itoh
{"title":"Distribution of long linear and branched polyamines in the thermophiles belonging to the domain Bacteria","authors":"K. Hamana, Ryuichi Hosoya, A. Yokota, M. Niitsu, H. Hayashi, T. Itoh","doi":"10.3118/JJSE.7.1.10","DOIUrl":"https://doi.org/10.3118/JJSE.7.1.10","url":null,"abstract":"Cellular polyamines of 44 newly validated eubacterial thermophiles growing at 45-80°C, belonging to eight orders (six phyla) of the domain Bacteria, were analyzed by HPLC and GC. A quaternary branched penta-amine, N4-bis(aminopropyl)norspermidine, was found in Hydrogenivirga and Sulfurihydrogenibium belonging to the order of Aquificales. Another quaternary branched penta-amine, N4-bis(aminopropyl) spermidine, was detected in Thermovibrio, Desulfurobacterium (Aquificales), Moorella, Desul-fovirgula (Thermoanaerobacteriales), Thermaerobacter (Clostridiales), Caldalkalibacillus, Thermobacillus (Bacillales), and Desulfothermus (Desulfovibrionales of the phylum Proteobacteria). The two branched penta- amines as well as linear penta- and hexa-amines were produced by Thermus species of the order Thermales. Marinitoga and Fervidobacterium (Thermotogales) contained linear penta-amines. The production of these long polyamines was dependent on culture temperature and remarkable at 70-80°C in the extreme/ hyper-thermophiles and to alkaline medium pH 8.5-9.0 in moderately thermophilic bacilli. The long polyamines were not detected in moderately thermophilic Anaerolinea (Anaerolineales of the phylum Chloroflexi). Distribution of either or both long linear polyamines and/or quaternary branched polyamines in the extreme/hyper-thermophiles is also reflected in their phylogenetical evolved locations.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127822585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
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