Journal of Japanese Society for Extremophiles最新文献_第2页

Temperature and salt dependent long linear and branched polyamine syntheses in the thermophiles belonging to the domain Archaea 古菌属嗜热菌中依赖温度和盐的长线状和支状多胺合成

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.7.1.21

K. Hamana, Ryuichi Hosoya, M. Niitsu, H. Hayashi, T. Itoh

引用次数: 3

Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution 定向进化改良海洋热藓耐热GH家族10木聚糖酶的嗜碱性

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.9.15

Wataru Tsukimura, K. Watanabe, C. Morokuma, R. Yatsunami, T. Fukui, Satoshi Nakamura

引用次数: 1

Device genetics for programmed cell death 程序性细胞死亡的设备遗传学

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.7.2.31

Y. Tashiro, M. Kobayashi

引用次数: 0

Use of a Thermus thermophilus host-vector system for expression of genes from the hyperthermophilic archaeon Pyrococcus horikoshii 利用嗜热热菌宿主载体系统表达嗜热古细菌堀氏焦球菌的基因

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.3.1_28

G. Takayama, T. Kosuge, S. Sunamura, I. Matsui, K. Ishikawa, A. Nakamura, T. Hoshino

{"title":"Use of a Thermus thermophilus host-vector system for expression of genes from the hyperthermophilic archaeon Pyrococcus horikoshii","authors":"G. Takayama, T. Kosuge, S. Sunamura, I. Matsui, K. Ishikawa, A. Nakamura, T. Hoshino","doi":"10.3118/JJSE.3.1_28","DOIUrl":"https://doi.org/10.3118/JJSE.3.1_28","url":null,"abstract":"Genes annotated to threonine dehydrogenase, α-mannosidase, and glutamate dehydrogenase of the hyperthermophilic archaeon Pyrococcus horikoshii OT3 were used to test the expression system of the thermophilic eubacterium Thermus thermophilus HB27. Using the previously described P215 promoter, the three genes were successfully expressed, but at significantly lower levels than in the Escherichia coli expression system with the T7 promoter. Replacement of the promoter region with P31 or Pslp_promoter improved the expression to a level comparable to or exceeding that in the E. coli system. Notably, α-mannosidase activity was clearly detected with the P31 and Pslp_promoters, which could not be detected in the E. coli system. Moreover, with 6 x His-tag fusions of these enzymes at their COOH- or NH2-termini, these proteins could be detected in the crude extracts of T. thermophilus by Western blotting, indicating that the increment of each enzyme activity was actually the result of enzyme production. These results demonstrate that the host-vector system of T. thermophilus is useful for expression of genes from hyperthermophiles.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124231838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Enhancing tolerance of rice to alkaline soils using genes involved in Fe acquisition in plants. 利用植物铁获取基因增强水稻对碱性土壤的耐受性。

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.7.2.15

N. Nishizawa

{"title":"Enhancing tolerance of rice to alkaline soils using genes involved in Fe acquisition in plants.","authors":"N. Nishizawa","doi":"10.3118/JJSE.7.2.15","DOIUrl":"https://doi.org/10.3118/JJSE.7.2.15","url":null,"abstract":"One of the widest ranging abiotic stresses in world agriculture arises from low iron (Fe) availability due to high soil pH, with 30% of arable land too alkaline for optimal crop production. To aquire Fe, Graminaceous plants use a chelation strategy and release phytosiderophores from their roots to chelate Fe (III) in the soil. Non-graminaceous plants differ from graminaceous plants in acquiring Fe, ferric reduction on root surface being the first step of iron uptake. Rice is very susceptible to low Fe availability. We enhanced the tolerance of rice to Fe deficiency in calcareous soils by introducing the barley genes participating in the phytosiderophore synthesis and tested these transgenic rice lines in a field experiment on a calcareous soil under paddy conditions. We showed that introducing barley genes involved in the synthesis of phytosiderophore into rice is an effective, practical method to improve agricultural productivity in calcareous soils. We also improved the tolerance of rice to Fe deficiency by increasing the ferric reductase activity of root cells by introducing the engineered gene refre1-372. To understand the molecular mechanisms that regulate Fe acquisition in plants, we analyzed the promoter region of the barley Fe deficiency inducible IDS2 gene and identified two novel cis-acting elements, IDE1 and IDE2. We identified two rice transcription factors, IDEF1 and IDEF2, which specifically bind to IDE1 and IDE2, respectively. The enhanced expression of transcription factors resulted in increased phytosiderophore secretion and tolerance to low Fe availability in a calcareous soil.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124617833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Andriashevia natsushimae, a new species of eelpout (Pisces, Perciformes: Zoarcidae) from Sagami Bay, Japan, and its phylogenic status as inferred from 16S rRNA 日本相模湾鳗鲡新种(双鱼，鲈形目:鱼科)Andriashevia natsushimae的鉴定及其16S rRNA的系统发育地位

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.8.20

Y. Nishiguchi, T. Miwa, S. Kubota, M. Taru, M. Okada

引用次数: 1

Physiological properties of Pseudomonas sp. strain MT-1, denitrifier from the 11,000 m-depth of Mariana Trench 马里亚纳海沟11000 m深度反硝化菌MT-1假单胞菌的生理特性

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.4.25

H. Tamegai, S. Nakamura, M. Miyazaki, Y. Nogi, R. Kasahara, C. Kato, K. Horikoshi

引用次数: 5

The symbiotic mechanism between the chemoautotrophic symbiotic bacteria and its host deep-sea Calyptogena clams, and the reductive genome evolution of these symbionts 趋化自养共生细菌与寄主深海蛤的共生机制及其还原性基因组进化

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.7.2.29

H. Kuwahara

{"title":"The symbiotic mechanism between the chemoautotrophic symbiotic bacteria and its host deep-sea Calyptogena clams, and the reductive genome evolution of these symbionts","authors":"H. Kuwahara","doi":"10.3118/JJSE.7.2.29","DOIUrl":"https://doi.org/10.3118/JJSE.7.2.29","url":null,"abstract":"Dense animal communities at hydrothermal vents and cold seeps rely on symbioses with chemoautotrophic bacteria. To reveal the symbiotic mechanism, we sequenced the genome of chemoautotrophic intracellular symbiont in deep-sea clam, Calyptogena okutanii. On the other hand, C. magnifica symbiont genome sequenced in USA. The genomes appear to have been reduced. To understand their reductive genome evolution (RGE), we compared their genomes. They have small genomes containing chemoautotrophic and intracellular symbiotic features, and lack most genes for DNA recombination and repair e.g. recA and mutY. Their genome structures were highly conserved excepting one inversion. Many deletions from small (1 kbp up to 11 kbp) sizes were detected and deletion numbers decreased exponentially with size. Densities of deletions, short-repeat density and A+T content were higher in non-coding regions than in coding regions. Because Calyptogena symbiont genomes lack recA, we deduced that deletions and the inversion occurred by RecA-independent recombination (RIR) at short-repeats with simultaneous consumption of repeats, and that short-repeats were regenerated by high rate of mutation with enhanced A+T bias in the absence of mutY. We proposed that an active RGE is ongoing by short-repeats dependent RIR with regeneration of short-repeats in extant Calyptogena symbionts.","PeriodicalId":204480,"journal":{"name":"Journal of Japanese Society for Extremophiles","volume":"69 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129139524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microbial tetraether lipids as an environmental tracer 微生物四醚脂作为环境示踪剂

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.6.45

T. Kitajima

引用次数: 0

Identification and characterization of the stator-force generator, MotPS, for motility of alkaliphilic Bacillus pseudofirumus OF4 嗜碱假假芽孢杆菌OF4的定子力发生器mops的鉴定与特性

Journal of Japanese Society for Extremophiles Pub Date : 1900-01-01 DOI: 10.3118/JJSE.3.1_42

政博伊藤

引用次数: 0