Phytopathology最新文献_第2页

Canker and dieback of Alnus rubra is caused by Lonsdalea quercina. 红叶石楠的腐烂和枯死是由 Lonsdalea quercina 引起的。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-29 DOI: 10.1094/PHYTO-06-24-0192-SC

Fernanda Iruegas-Bocardo, Wendy Sutton, Riley A Buchanan, Niklaus J Grünwald, Jeff H Chang, Melodie L Putnam

{"title":"Canker and dieback of Alnus rubra is caused by Lonsdalea quercina.","authors":"Fernanda Iruegas-Bocardo, Wendy Sutton, Riley A Buchanan, Niklaus J Grünwald, Jeff H Chang, Melodie L Putnam","doi":"10.1094/PHYTO-06-24-0192-SC","DOIUrl":"10.1094/PHYTO-06-24-0192-SC","url":null,"abstract":"Understanding the ecology of pathogens is important for disease management. Recently a devastating canker disease was found on red alder (Alnus rubra) planted as landscape trees. Bacteria were isolated from two groups of symptomatic trees located approximately 1 kilometer apart and one strain from each group was used to complete Koch's postulates. Results showed that these bacteria can not only cause disease on red alder but also on two other alder species. Unexpectedly, analyses of genome sequences of bacterial strains identified them as Lonsdalea quercina, a pathogenic species previously known to cause dieback of oak species, but not alder. Additionally, a core genome phylogeny clustered bacterial strains isolated from red alder within a subclade of L. quercina strains isolated from symptomatic oak trees. Consistent with the close phylogenetic relationship, there was no obvious evidence for divergence in genome composition of strains isolated from red alder and oak. Altogether, findings indicate that L. quercina is a potential threat to Alnus species.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-component resistance responses of melon to zucchini yellow mosaic virus. 甜瓜对西葫芦黄镶嵌病毒的多成分抗性反应。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-29 DOI: 10.1094/PHYTO-05-24-0166-R

Líllian B J Bibiano, Camila P Carvalho, Ricardo Gioria, Jorge A M Rezende, Luis E A Camargo

{"title":"Multi-component resistance responses of melon to zucchini yellow mosaic virus.","authors":"Líllian B J Bibiano, Camila P Carvalho, Ricardo Gioria, Jorge A M Rezende, Luis E A Camargo","doi":"10.1094/PHYTO-05-24-0166-R","DOIUrl":"https://doi.org/10.1094/PHYTO-05-24-0166-R","url":null,"abstract":"A major resistance gene of the melon accession PI414723 to zucchini yellow mosaic virus (ZYMV) is located at the Zym-1 locus on chromosome 2, but the underlying defense mechanisms are poorly understood. The physiological responses and expression of selected genes at Zym-1 were assessed in PI414723 and in the susceptible genotype Védrantais. Viral titers and the expression of genes related to systemic acquired resistance (SAR) were evaluated in inoculated (Inoc) and non-inoculated (Non-Inoc) portions of the cotyledons at 3, 7 and 10 days after inoculation (dai) and in apical leaves at 10 dai. ZYMV was detected in both portions of the cotyledons but not in the apical leaves of PI414723 plants. Also, ZYMV was recovered in a susceptible zucchini only from Inoc portions at 3 dai. By contrast, in Védrantais ZYMV was detected and recovered from all tissues at high concentrations. Starchy local lesions and accumulation of transcripts of the SAR marker genes PR1 and PR4 were also detected in the resistant genotype. Plus, transcripts of one candidate resistance gene analog previously located at Zym-1 and of two melon homologs of restricted tobacco etch virus movement 2 (RTM2) genes located close to Zym-1, accumulated only in PI414723. It is proposed that resistance results from the combined action of the R gene, involved in restricting ZYMV replication after a supposed recognition event and of the RTM genes which impact viral systemic movement to distal apical tissues.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic approach for identifying unique genomic sequences for Fusarium oxysporum f. sp. lactucae race 1 and development of molecular diagnostic tools. 确定 Fusarium oxysporum f. sp. lactucae race 1 的独特基因组序列和开发分子诊断工具的系统方法。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-24 DOI: 10.1094/PHYTO-04-24-0142-R

Ningxiao Li, David M Geiser, Jacob L Steenwyk, Cayla Tsuchida, Steve Koike, Stephanie Slinski, Frank N Martin

{"title":"A systematic approach for identifying unique genomic sequences for Fusarium oxysporum f. sp. lactucae race 1 and development of molecular diagnostic tools.","authors":"Ningxiao Li, David M Geiser, Jacob L Steenwyk, Cayla Tsuchida, Steve Koike, Stephanie Slinski, Frank N Martin","doi":"10.1094/PHYTO-04-24-0142-R","DOIUrl":"https://doi.org/10.1094/PHYTO-04-24-0142-R","url":null,"abstract":"Fusarium oxysporum f. sp. lactucae (FOLac) is a soil- and seedborne fungal pathogen that causes Fusarium wilt of lettuce, an important disease threatening global lettuce production. Based on pathogenicity on differential lettuce cultivars, four races (1-4) have been identified, with race 1 the only race detected in the United States, and the closely related, emerging race 4 known only in Europe. The development of race-specific diagnostic tools is hindered by insufficient genomic data to distinguish between the two races and FOLac from other F. oxysporum formae speciales and nonpathogenic isolates. Here, we describe a systematic approach for developing diagnostic markers for FOLac race 1 that utilized a comprehensive sequence database of F. oxysporum to identify 15 unique genomic sequences. Marker specificity was validated through an exhaustive screening process against genomic data from 797 F. oxysporum isolates representing 64 formae speciales and various plants and non-plant substrates. One of the unique sequences was used to develop a TaqMan quantitative polymerase chain reaction assay and a recombinase polymerase amplification assay, both exhibiting 100% sensitivity and specificity when tested against purified DNA from 171 F. oxysporum isolates and 69 lettuce samples. The relationship between qPCR Ct values and colony forming units (CFU)/g values was also determined. This study not only introduces a new marker for FOLac race 1 diagnostics and soil quantitation, but also underscores the value of an extensive genomic database and screening software pipeline for developing molecular diagnostics for F. oxysporum formae speciales and other fungal taxa.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenazine-1-carboxamide Regulates Pyruvate Dehydrogenase of Phytopathogenic Fungi to Control Tea Leaf Spot Caused by Didymella segeticola. 吩嗪-1-甲酰胺调节植物病原真菌的丙酮酸脱氢酶，以控制由Didymella segeticola引起的茶叶斑点病。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-22 DOI: 10.1094/PHYTO-07-24-0209-R

Zeqi Qi, Fenghua Liu, Dongxue Li, Jiayu Yin, Delu Wang, Nazeer Ahmed, Yue Ma, Jing-Jiang Zhou, Zhuo Chen

{"title":"Phenazine-1-carboxamide Regulates Pyruvate Dehydrogenase of Phytopathogenic Fungi to Control Tea Leaf Spot Caused by Didymella segeticola.","authors":"Zeqi Qi, Fenghua Liu, Dongxue Li, Jiayu Yin, Delu Wang, Nazeer Ahmed, Yue Ma, Jing-Jiang Zhou, Zhuo Chen","doi":"10.1094/PHYTO-07-24-0209-R","DOIUrl":"https://doi.org/10.1094/PHYTO-07-24-0209-R","url":null,"abstract":"Due to a lack of understanding of the disease epidemiology and comprehensive control measures, tea leaf spot caused by Didymella segeticola has a significant negative impact on tea yield and quality in the tea plantations of Southwest China. Phenazine-1-carboxamide (PCN) is a phenazine compound derived from Pseudomonas species, which exhibits antimicrobial activity against various pathogens. However, its inhibitory mechanism is not yet clear. The current study evaluated the inhibitory activity of PCN against various phytopathogenic fungi and found that PCN has inhibitory activity against multiple pathogens, with a half-maximal effective concentration (EC50) value for D. segeticola of 16.11 μg/mL in vitro and a maximum in-vivo curative activity of 72.28% toward tea leaf spot. Morphological changes in the hyphae after exposure to PCN were observed through microstructure and ultrastructure analysis, and indicated that PCN causes abnormalities in the hyphae, such as cytoplasmic coagulation, shortened hyphal inter-septum distances, and unclear boundaries of organelles. Transcriptomic analysis revealed that PCN upregulated the expression of genes related with energy metabolism. PCN significantly reduced the ATP concentration in the hyphae and decreased mitochondrial membrane potential. Molecular docking analysis indicated that PCN binds to one of the candidate target proteins, pyruvate dehydrogenase, with lower free energy of -10.7 kcal/mol. This study indicated that PCN can interfere with energy metabolism, reducing ATP generation, ultimately affecting hyphal growth. Overall, PCN shows potential for future application in the control of tea leaf spot due to its excellent antifungal activity and unique mode of action.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Microbial Metabolite Wuyiencin Potential Targets Threonine dehydratase in Didymella segeticola to Achieve Control of Tea Leaf Spot. 微生物代谢物武夷菌素能潜在地靶向半知菌（Didymella segeticola）体内的苏氨酸脱水酶，从而达到防治茶叶斑点病的目的。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-22 DOI: 10.1094/PHYTO-06-24-0200-R

Youli Ma, Peiying Li, Wenjing Xie, Fenghua Liu, Dongxue Li, Atta Ur Rehman, Delu Wang, Jing-Jiang Zhou, Yue Ma, Zhuo Chen

{"title":"The Microbial Metabolite Wuyiencin Potential Targets Threonine dehydratase in Didymella segeticola to Achieve Control of Tea Leaf Spot.","authors":"Youli Ma, Peiying Li, Wenjing Xie, Fenghua Liu, Dongxue Li, Atta Ur Rehman, Delu Wang, Jing-Jiang Zhou, Yue Ma, Zhuo Chen","doi":"10.1094/PHYTO-06-24-0200-R","DOIUrl":"https://doi.org/10.1094/PHYTO-06-24-0200-R","url":null,"abstract":"Tea leaf spot caused by Didymella segeticola is a disease that has recently been discovered in the tea plantations of Southwest China, and which has a significant negative impact on the yield and quality of tea leaves. Wuyiencin is a nucleotide antimicrobial that is effective against a range of fungal diseases. However, its mode of action is still unclear. The current study found that wuyiencin inhibited the mycelial growth of D. segeticola in vitro. Meanwhile, in vivo experiments confirmed that wuyiencin had a significant curative effect on tea leaf spot. Microscopic observation represented it damaged the organelles and nucleus in fungal cells. Reverse transcription quantitative PCR assays revealed that mycelium treated with wuyiencin at the half-maximal effective concentration (EC50) dosage for 1 hour exhibited 3.23 times lower expression of Threonine dehydratase (Td) gene, which is responsible for producing pyruvate. The wild type (WT) strain had a 1.77-fold higher pyruvate concentration than that in the td mutant (P < 0.05). The td mutant was more sensitive than the WT to wuyiencin treatment, with the EC50 value in the td mutant being 30.01 μg/ml, compared with 82.34 μg/ml in the WT. Molecular docking demonstrated that wuyiencin bound to Td, with a binding energy of -10.47 kcal/mol. Compared with the WT strain, wuyiencin significantly reduced ATP concentration of the td mutant strain at dosages of 80.0 and 160.0 µg/ml. In total, wuyiencin reduced Td activity, inhibited pyruvate production, and decreased ATP content in the phytopathogenic fungus, ultimately disturbing the growth of the mycelium.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142506496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biphenyl and dibenzofuran phytoalexins differentially inhibit root-associated microbiota in apple, including fungal and oomycetal replant disease pathogens. 联苯和二苯并呋喃类植物毒素对苹果根部相关微生物群（包括真菌和卵菌再植病病原体）的抑制作用不同。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-21 DOI: 10.1094/PHYTO-03-24-0088-R

Belnaser A Busnena, Ludger Beerhues, Benye Liu

{"title":"Biphenyl and dibenzofuran phytoalexins differentially inhibit root-associated microbiota in apple, including fungal and oomycetal replant disease pathogens.","authors":"Belnaser A Busnena, Ludger Beerhues, Benye Liu","doi":"10.1094/PHYTO-03-24-0088-R","DOIUrl":"https://doi.org/10.1094/PHYTO-03-24-0088-R","url":null,"abstract":"Apple replant disease (ARD) is a serious soilborne disease in apple nurseries and orchards worldwide. ARD is the result of an unbalanced soil microbiome in which multiple soil-borne plant pathogenic fungi, oomycetes and nematodes form a disease complex. Biphenyl and dibenzofuran phytoalexins are found in greater quantities in the roots of apple plants grown in ARD soil compared to disinfected ARD soil. However, the contribution of these compounds to plant health or disease is not yet understood. Here, the antimicrobial activity of fourteen chemically synthesized biphenyl and dibenzofuran phytoalexins was tested against eight selected microorganisms isolated from either rhizosphere soils or apple roots. These included five potentially beneficial bacteria (Rhodococcus pseudokoreensis strain R79T, Rhodococcus koreensis strain R85, Streptomyces pulveraceus strain ES16, Streptomyces ciscaucasicus GS2, Priestia megaterium strain B1), two ARD fungal pathogens (Ilyonectria robusta H131 and Dactylonectria torresensis N3) and one oomycete (Globisporangium terrestre). Two phytoalexin mixtures reflecting the percentages of the individual compounds inside the roots (Mixture A) and the root exudate (Mixture B) were also tested. The two phytoalexin mixtures demonstrated a higher antimicrobial activity than the individual phytoalexins, suggesting a synergistic effect. The minimum inhibitory concentration (MIC) and the half maximal effective concentration (EC50) values determined to be active against the eight microbes were within a range of 2.5-fold the ecologically relevant phytoalexin concentration (approximately 33 and 24 µg ml-1 in roots and exudates, respectively). The results contribute to our understanding of the impact of apple root phytoalexins on ARD and suggest potential strategies for disease management.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loop-mediated isothermal amplification detection of Phytophthora kernoviae, Phytophthora ramorum, and the P. ramorum NA1 lineage on a microfluidic chip and smartphone platform. 在微流控芯片和智能手机平台上进行环介导等温扩增检测噬菌体（Phytophthora kernoviae）、根瘤蚜（Phytophthora ramorum）和根瘤蚜 NA1 系。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-21 DOI: 10.1094/PHYTO-02-24-0055-R

Amanda Mainello-Land, Amanda C Saville, Jyotsna Acharya, Jean Ristaino

{"title":"Loop-mediated isothermal amplification detection of Phytophthora kernoviae, Phytophthora ramorum, and the P. ramorum NA1 lineage on a microfluidic chip and smartphone platform.","authors":"Amanda Mainello-Land, Amanda C Saville, Jyotsna Acharya, Jean Ristaino","doi":"10.1094/PHYTO-02-24-0055-R","DOIUrl":"https://doi.org/10.1094/PHYTO-02-24-0055-R","url":null,"abstract":"Rapid, field-deployable assays such as loop-mediated isothermal amplification (LAMP) are critical for detecting nursery and forest pathogens like Phytophthora ramorum and P. kernoviae to prevent pathogen spread. We developed and validated four LAMP assays for genus-level detection of Phytophthora spp., species-level detection of P. kernoviae and P. ramorum and lineage-level detection of the P. ramorum NA1 lineage. Cross reactivity of the two species-specific LAMP assays was evaluated using a set of 18 Phytophthora spp. known to infect nursery crop hosts. The correct target species were detected by the species-level LAMP assays. The Phytophthora spp. LAMP assay was evaluated against 27 Phytophthora spp. and other bacterial and fungal pathogens and reacted with all the Phytophthora spp. evaluated but no other bacterial or fungal species. The limit of detection (LOD) of the P. kernoviae LAMP was 100 fg/µl and the LOD of the P. ramorum LAMP assay was 1 pg/µl of DNA. The NA1 LAMP assay was tested against the NA1, NA2, EU1, and EU2 lineages of P. ramorum and was lineage-specific but had a higher LOD (100pg/µl) than the species-specific LAMP assays. Both P. ramorum and P. kernoviae LAMP assays were highly precise (>0.94) in detecting the respective pathogens in symptomatic rhododendron leaves and co-inoculation experiments. The set of four LAMP assays were run in tandem on a microfluidic chip and smartphone platform and can be used in the field to detect and monitor spread of these regulatory Phytophthora spp. in forest and/or nursery settings.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Fusarium isolates from Upland cotton roots in Uzbekistan and whole-genome comparison with isolates from the USA. 乌兹别克斯坦陆地棉根部镰刀菌分离物的分子特征以及与美国分离物的全基因组比较。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-10 DOI: 10.1094/PHYTO-04-24-0152-R

Timothy O Jobe, Ibrokhim Y Abdurakhmonov, Mauricio Ulloa, Mohamed Fokar, Zabardast T Buriev, Shukhrat E Shermatov, Abdusalom K Makamov, Dilshod E Usmanov, Mukhtor M Darmanov, Kirk Broders, Margaret L Ellis

{"title":"Molecular characterization of Fusarium isolates from Upland cotton roots in Uzbekistan and whole-genome comparison with isolates from the USA.","authors":"Timothy O Jobe, Ibrokhim Y Abdurakhmonov, Mauricio Ulloa, Mohamed Fokar, Zabardast T Buriev, Shukhrat E Shermatov, Abdusalom K Makamov, Dilshod E Usmanov, Mukhtor M Darmanov, Kirk Broders, Margaret L Ellis","doi":"10.1094/PHYTO-04-24-0152-R","DOIUrl":"https://doi.org/10.1094/PHYTO-04-24-0152-R","url":null,"abstract":"Fusarium oxysporum f. sp. vasinfectum (FOV) is a significant cotton (Gossypium spp.) pathogen causing vascular wilt, browning of the vascular tissues, and plant death in the most severe cases. This global disease is responsible for sizeable crop losses annually and is found in many cotton producing regions, including the Republic of Uzbekistan and the USA. Specifically, FOV race 4 (FOV4) has been disrupting production for years. This study aimed to genetically characterize FOV4 isolates causing disease in the main cotton producing region of Uzbekistan and compare with FOV4 isolates from the USA. A field study conducted in the Bukhara region of the Republic of Uzbekistan in the spring of 2022 identified both FOV4 and new Fusarium isolates from Upland cotton exhibiting typical Fusarium wilt symptoms. Molecular markers were initially used to identify isolates of interest, and a phylogenetic analysis was performed using partial EF1-α sequences, followed by a comparative genomic analysis. We also report for the first time the isolation of F. solani and F. commune causing Fusarium wilt in Uzbekistan. Furthermore, we show that the FOV4 population within our sampling region of Uzbekistan may be dominated by a single biotype with an effector profile similar to that of FOV race 7 (FOV7). One of these effector proteins is also present in the F. commune isolate showing virulence to cotton. Whole genome comparisons between FOV races can identify unique genetic markers for FOV4 and aid in the development of tools for breeding FOV resistant cotton varieties.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated analysis of the Passifloraceae virome using public-domain data. 利用公共域数据综合分析西番莲科植物病毒群。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-10 DOI: 10.1094/PHYTO-08-24-0269-FI

Monica Higuita, Juliana Sanchez-Yali, Alejandra Perez, Engie Arias, Pablo A Gutierrez

{"title":"An integrated analysis of the Passifloraceae virome using public-domain data.","authors":"Monica Higuita, Juliana Sanchez-Yali, Alejandra Perez, Engie Arias, Pablo A Gutierrez","doi":"10.1094/PHYTO-08-24-0269-FI","DOIUrl":"https://doi.org/10.1094/PHYTO-08-24-0269-FI","url":null,"abstract":"Passifloraceae is a plant family that includes several species of interest in the food, medicinal, and ornamental industries. The most relevant species are the purple and yellow varieties of P. edulis, which are among the most highly prized tropical fruits in the international markets. Unfortunately, the rapid expansion of this crop worldwide has resulted in the emergence of several viral diseases that endangered the productivity of this crop. In this work, we performed an integrated analysis of the Passifloraceae virome using public data. We investigated Pubmed and Genbank records and analyzed all the transcriptome data available for members of this plant family. This analysis resulted in the identification of six novel virus associations and six putative new viral species. We also used RNAseq to inspect virus accumulation levels and mixed infections. Using network analysis, we also examined the global distribution of Passiflora viruses and their associations with alternative hosts, which is valuable information in implementing viral disease management strategies. Our data suggest that a large diversity of viruses remains to be discovered. Finally, we used the information gathered in this work to estimate the cross-transmission risk of viruses in Colombian Passiflora fields.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New, complete circularized genomes of Xanthomonas citri pv. mangiferaeindicae produced from short- and long-read co-assembly shed light on strains that emerged a decade ago on mango and cashew in Burkina Faso. 新的、完整的柠檬黄单胞菌（Xanthomonas citri pv. mangiferaeindicae）环化基因组是通过短读码和长读码联合组装产生的，它揭示了十年前在布基纳法索芒果和腰果上出现的菌株。

IF 2.6 2区农林科学

Phytopathology Pub Date : 2024-10-10 DOI: 10.1094/PHYTO-08-24-0267-SC

Claudine Boyer, Pierre Lefeuvre, Cyrille Zombre, Adrien Rieux, Issa Wonni, Lionel Gagnevin, Olivier Pruvost

{"title":"New, complete circularized genomes of Xanthomonas citri pv. mangiferaeindicae produced from short- and long-read co-assembly shed light on strains that emerged a decade ago on mango and cashew in Burkina Faso.","authors":"Claudine Boyer, Pierre Lefeuvre, Cyrille Zombre, Adrien Rieux, Issa Wonni, Lionel Gagnevin, Olivier Pruvost","doi":"10.1094/PHYTO-08-24-0267-SC","DOIUrl":"https://doi.org/10.1094/PHYTO-08-24-0267-SC","url":null,"abstract":"We report high-quality genomes of three strains of Xanthomonas citri pv. mangiferaeindicae (Xcm), the causal agent of mango bacterial canker disease, including the pathotype strain of this pathovar and two strains from Burkina Faso that emerged a decade ago. These strains hosted two to three plasmids of sizes ranging from 19 to 86 kb. Genome mining revealed the presence of several secretion systems (SS) and effectors involved in virulence of xanthomonads with (i) a T1SS of the hlyDB group, (ii) xps and xcs T2SSs, (iii) a T3SS with several type three effectors (T3E), including transcription activator-like effectors (TALE), (iv) several T4SSs associated with plasmid or integrative conjugative elements (ICE) mobility, (v) three T5SS subclasses (Va, Vb and Vc) and (vi) a single i3* T6SS. The two strains isolated in Burkina Faso from mango (Mangifera indica L.) and cashew (Anacardium occidentale L.) differed by only 14 SNPs and shared identical secretion systems and T3E repertoire. Several TALEs were identified in each strain, some of which may target plant genes previously found implicated in disease development in other xanthomonad-associated pathosystems. These results support the emergence in Burkina Faso a decade ago of very closely related strains that became epidemic on mango and cashew, i.e., two distinct host genera of a same plant family. These new genomic resources will contribute to better understand the biology and evolution of this agriculturally major crop pathogen.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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