Phytochemistry Letters最新文献

{"title":"Daphnicalycinones A and B, Daphniphyllum alkaloids from the roots of Daphniphyllum calycinum with anti-inflammatory activity","authors":"Jiaqi Guo ,&nbsp;Wenlong Li ,&nbsp;Runchao Ma ,&nbsp;Lizhu Han ,&nbsp;Jiani Ying ,&nbsp;Xiongyu Meng ,&nbsp;Huaqiang Li ,&nbsp;Qi Shi ,&nbsp;Luping Qin","doi":"10.1016/j.phytol.2025.102946","DOIUrl":"10.1016/j.phytol.2025.102946","url":null,"abstract":"<div><div>Two undescribed <em>Daphniphyllum</em> alkaloids, daphnicalycinones A and B (<strong>1</strong> and <strong>2</strong>), together with three known analogues, daphnezomine D (<strong>3</strong>), calycindaphine H (<strong>4</strong>) and calycindaphine A (<strong>5</strong>) were isolated from the roots of <em>Daphniphyllum calycinum</em>. Their planar structures and stereo-structures were determined by extensively spectral analyses, including HRESIMS, NMR, DP4 + computational method and single-crystal X-ray crystallography. The anti-inflammatory activities of compounds <strong>1</strong>−<strong>5</strong> were tested on LPS-induced RAW 264.7 cells, and the results showed that <strong>2</strong> and <strong>5</strong> exhibited potent inhibitory effects with the IC₅₀ values of 1.53 ± 0.35 <em>μ</em>M and 10.13 ± 0.72 <em>μ</em>M, respectively.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"67 ","pages":"Article 102946"},"PeriodicalIF":1.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted deep eutectic solvent extraction of kukoamine A and kukoamine B from Lycii cortex","authors":"Xiaomei Wang ,&nbsp;Jinxia Hu ,&nbsp;Ling Ma ,&nbsp;Zhiming Han ,&nbsp;Jianfei Liu ,&nbsp;Zhibin Lu ,&nbsp;Dong Pei ,&nbsp;Ningli Wang ,&nbsp;Duolong Di","doi":"10.1016/j.phytol.2025.02.013","DOIUrl":"10.1016/j.phytol.2025.02.013","url":null,"abstract":"<div><div>Deep eutectic solvents (DES), are new environmentally friendly and efficient extraction solvents, and have considerable importance for environmental protection and sustainable development. In this study, ultrasound-assisted extraction (UAE) using DES was carried out to extract kukoamine A (KuA) and kukoamine B (KuB) from <em>Lycii cortex</em> (LyC). The DES was synthesised using a magnetic stirring method. A novel approach to extract KuA and KuB from LyC was developed by combining UAE with DES. Different DES formulations were tested to evaluate the efficiency of KuA and KuB extraction. Among them, choline chloride-lactic acid (ChCl-LA) DES had the best effect, and its extraction rate was much higher than that of methanol, which was 8.5 times that of methanol. Both single factorial experiments and response surface methodology (RSM) were used to optimise and model the extraction process. Density functional theory was also used to discuss the extraction mechanism. It was found that HBD and HBA will interact with hydrogen bonding and van der Waals interactions during the formation of DES, and these interactions will also affect the decrease in electron density. Taking ChCl-LA and ChCl-urea as examples, it was found that the binding energy of ChCl-LA was lower than that of ChCl-urea, indicating that ChCl-LA had a stronger interaction with the target substance and a higher extraction rate. The combination of UAE-DES proved to be a sustainable, effective, and simple extraction technique for the recovery of KuA and KuB from LyC.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 136-143"},"PeriodicalIF":1.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new cytosporin derivatives from the deep-sea-derived fungus Eutypella scoparia FS716","authors":"TingYue Wen ,&nbsp;YuChan Chen ,&nbsp;JiaXi Li ,&nbsp;HongXin Liu ,&nbsp;WeiMin Zhang ,&nbsp;XiaoXia Gao","doi":"10.1016/j.phytol.2025.02.014","DOIUrl":"10.1016/j.phytol.2025.02.014","url":null,"abstract":"<div><div>Two new cytosporin derivatives, cytosporin Y₄ (<strong>1</strong>) and cytosporin Y₅ (<strong>2</strong>), together with seven known compounds (<strong>3–9</strong>) were isolated from <em>Eutypella scoparia</em> FS716, a fungus from the deep-sea sediment in South China Sea. The chemical structures of compounds <strong>1</strong> and <strong>2</strong> were established by 1D, 2D NMR, HRESIMIS, IR and UV spectroscopic data analyses. All the isolated compounds were evaluated for cytotoxic and anti-inflammatory activities. As a result, compounds <strong>3–6, 8</strong> and <strong>9</strong> showed significant anti-inflammatory activities.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 131-135"},"PeriodicalIF":1.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiplasmodial-guided isolation of constituents from the stem bark of Balanites aegyptiaca","authors":"Sylvestre Saidou Tsila ,&nbsp;Claire Christine Waleguele ,&nbsp;Noella Molisa Efange ,&nbsp;Billy Toussie Tchegnitegni ,&nbsp;Jean Rodolphe Chouna ,&nbsp;Angelbert Fusi Awantu ,&nbsp;Yannick Stéphane Fotsing Fongang ,&nbsp;Lawrence Ayong ,&nbsp;Norbert Sewald ,&nbsp;Bruno Ndjakou Lenta","doi":"10.1016/j.phytol.2025.02.016","DOIUrl":"10.1016/j.phytol.2025.02.016","url":null,"abstract":"<div><div>The CH₂Cl₂/MeOH (1:1, v/v) extract of the stem bark of <em>Balanites aegyptiaca</em> showed moderate antiplasmodial activity against both the chloroquine sensitive <em>Plasmodium falciparum</em> 3D7 (<em>Pf</em>3D7) and multidrug-resistant <em>Plasmodium falciparum</em> Dd2 (<em>Pf</em>Dd2) strains with IC₅₀ values of 14.20 ± 1.92 and 42.14 ± 2.83 <em>µ</em>g/mL, respectively. The extract was successively subjected to liquid-liquid partition with <em>n-</em>hexane, ethyl acetate, and <em>n-</em>butanol, and the fractions obtained were assessed for their antiplasmodial activity against the same strains. The ethyl acetate soluble fraction was the most active one with IC₅₀ values of 7.74 ± 0.74 and 19.27 ± 2.15 <em>µ</em>g/mL against <em>Pf</em>3D7 and <em>Pf</em>Dd2, respectively, followed by the <em>n-</em>hexane fraction (IC₅₀ = values of 31.06 ± 2.60 and 90.67 ± 0.07 <em>µ</em>g/mL against <em>Pf</em>3D7 and <em>Pf</em>Dd2, respectively). The <em>n-</em>butanol fraction was inactive (IC₅₀ &gt; 50 <em>µ</em>g/mL). Successive column chromatography of the active fractions led to the isolation of fifteen compounds including two new lignans [balanitals A (<strong>1</strong>) and B (<strong>2</strong>)], together with 13 known compounds (<strong>3</strong>–<strong>15</strong>). Their structures were established by means of spectroscopic methods. The isolated compounds were also assessed for their antiplasmodial activity against both <em>Pf</em>3D7 and <em>Pf</em>Dd2. Compound <strong>4</strong> exhibited a good antiplasmodial activity with an IC₅₀ value of 4.95 ± 0.70 <em>µ</em>M against <em>Pf</em>3D7, while it was not active against <em>Pf</em>Dd2 (IC₅₀ &gt; 100 <em>µ</em>M).</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 126-130"},"PeriodicalIF":1.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new compounds from Pentadesma butyracea and their biological activities","authors":"Yue Yang,&nbsp;Li Hua Gong,&nbsp;Fu Hua Peng,&nbsp;Tao Jiang,&nbsp;Jian Guo Hu,&nbsp;Hong Dong Liu,&nbsp;Bin Li,&nbsp;Yin Xia Yang,&nbsp;Jing Ying Peng,&nbsp;Xue Mei Gao","doi":"10.1016/j.phytol.2025.02.012","DOIUrl":"10.1016/j.phytol.2025.02.012","url":null,"abstract":"<div><div>Two new compounds (<strong>1</strong>-<strong>2</strong>) and twenty-two known compounds (<strong>3</strong>-<strong>24</strong>) were isolated from the ethanol extract of the stems and leaves of <em>Pentadesma butyracea</em> Sabine (Clusiaceae). The structures of compounds <strong>1</strong>-<strong>2</strong> were elucidated by spectroscopic methods, including extensive 1D- and 2D-NMR spectroscopy and HRMS techniques. According to previous literature research and molecular docking, we tested antiproliferative and <em>α</em>-glucosidase inhibitory activities of these compounds. Compounds <strong>1</strong>, <strong>5</strong>, and <strong>6</strong> showed good <em>α</em>-glucosidase inhibitory activities with IC₅₀ values of 23.12, 3.60, and 16.83 <em>μ</em>M, respectively. Molecular docking results showed that compounds <strong>1</strong>, <strong>5</strong>, and <strong>6</strong> have strong binding affinities with <em>α</em>-glucosidase. In addition, the antiproliferative activities of compounds <strong>1</strong>, <strong>5</strong>, and <strong>6</strong> against human cancer A549 cells exhibited IC₅₀ values of 41.47, 19.46 and 45.75 <em>μ</em>M, respectively. Except for compound <strong>14</strong>, all the compounds were isolated from the genus <em>Pentadesma</em> for the first time. The findings propose that xanthones with isoprenyl groups may be effective lead compounds for the development of <em>α</em>-glucosidase inhibitors and anticancer therapies.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 119-125"},"PeriodicalIF":1.3,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three new cycloartane and friedelane triterpenoids from the leaves of Caloncoba flagelliflora","authors":"Mireille Yimtchui Towa ,&nbsp;Achille Nouga Bissoue ,&nbsp;Klev Gaïtan Sikam ,&nbsp;Gervais Happi Mouthé ,&nbsp;Moses K. Langat ,&nbsp;Eduard Mas-Claret ,&nbsp;Jean Duplex Wansi","doi":"10.1016/j.phytol.2025.02.007","DOIUrl":"10.1016/j.phytol.2025.02.007","url":null,"abstract":"<div><div>Three previously undescribed triterpenoids, caloncobic acid D (<strong>1</strong>), caloncobalactone D (<strong>2</strong>), and trichadenic acid C (<strong>3</strong>) were isolated from the leaves of <em>Caloncoba flagelliflora</em> (Mildbr.) Gilg ex Pellegr. Additionally, seven known compounds, trichadonic acid (<strong>4</strong>), glaucalactone C (<strong>5</strong>), glaucanoic acid (<strong>6</strong>), friedelin (<strong>7</strong>), sitosterol (<strong>8</strong>), and stigmasterol (<strong>9</strong>), along with stigmasterol 3-O-<em>β</em>-D-glucopyranoside (<strong>10</strong>), were identified. The structures of these compounds were elucidated using spectroscopic methods, including extensive 1D and 2D NMR techniques, chemical evidence, and comparison with literature data. Subsequently, compounds <strong>5–6</strong> and <strong>9–10</strong> were evaluated for antiplasmodial activity against two strains of <em>Plasmodium falciparum</em>: <em>Pf</em>Dd2 and <em>Pf</em>3D7. Compound <strong>6</strong> exhibited moderate activity, with IC₅₀ values of (7.5 ± 0.1 μM) and (10.0 ± 0.7 μM) against PfDd2 and Pf3D7, respectively. Compound <strong>10</strong> also moderately inhibited Pf3D7, with an IC₅₀ value of (7.5 ± 0.1 μM). These findings highlight C. <em>flagelliflora</em> as a valuable source of triterpenes and suggest their potential as candidates for new antiplasmodial drug development.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 105-110"},"PeriodicalIF":1.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pitsubcosides U−V, two sesquiterpenoid glycosides with multifunctional activities from Pittosporum subulisepalum","authors":"Wei-zhen Kong ,&nbsp;Gui-chun Yang ,&nbsp;Zhao-xuan Wang,&nbsp;Chao-hui Wang,&nbsp;Xiu-yun Zhang","doi":"10.1016/j.phytol.2025.02.015","DOIUrl":"10.1016/j.phytol.2025.02.015","url":null,"abstract":"<div><div>In our previous study, nineteen previously unreported antibacterial sesquiterpenoid glycosides, designated as pitsubcosides <strong>A−S</strong>, including twelve eudesmane-scaffold and seven cadinene-scaffold ones, were isolated from <em>Pittosporum subulisepalum.</em> To further explore structurally diverse bio-active metabolites, our research efforts were focused on the sub-fraction (Fr. E), from which two additional sesquiterpenoid glycosides, pitsubcosides U<strong>−</strong>V (<strong>1−2</strong>), were isolated alongside eleven known analogs. The structures of these compounds were elucidated through comprehensive spectroscopic analyses, including 1D and 2D NMR, HRESIMS, and calculated electronic circular dichroism (ECD) analysis. Notably, compounds <strong>1</strong> and <strong>2</strong> exhibited potent inhibitory effects against <em>Bacillus cereus</em> (<em>B. cereus</em>) with the minimum inhibitory concentration (MIC) values of 3.13 <em>μ</em>M and 6.25 <em>μ</em>M, respectively. Scanning electron microscopy (SEM) observations revealed the antibacterial mechanism, which involves either preventing biofilm formation or disrupting existing cell membranes. Furthermore, compound <strong>1</strong> showed anti-fungal activity against <em>Sclerotinia sclerotiorum</em> (<em>S. sclerotiorum</em>), achieving a relative inhibitory rate of 92.3 % at a concentration 100 <em>μ</em>M. Additionally, compound <strong>1</strong> showed inhibitory effects on the production of nitric oxide (NO) in the lipopolysaccharide (LPS)-induced BV-2 microglial cells by suppressing the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), with IC₅₀ value of 6.37 <em>μ</em>M. Western blot analysis and molecular docking simulation corroborated the anti-inflammatory properties of compound <strong>1</strong>. The results indicated that <em>P. subulisepalum</em> can be developed as potential antibacterial and anti-inflammatory resource.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 111-118"},"PeriodicalIF":1.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new phenolic glycosides from Cyathula officinalis and their relaxant effects on uterine smooth muscle","authors":"Mei-Ji Sun ,&nbsp;Rui Zhao ,&nbsp;Min Wen ,&nbsp;Chun-Wang Meng ,&nbsp;Hai-Guo Su ,&nbsp;Fu-Xin Mi ,&nbsp;Cheng Peng ,&nbsp;Juan Liu ,&nbsp;Liang Xiong","doi":"10.1016/j.phytol.2025.02.010","DOIUrl":"10.1016/j.phytol.2025.02.010","url":null,"abstract":"<div><div>Two novel phenolic glycosides, 3-methoxyacetophenone-4-<em>O</em>-<em>β</em>-D-apiofuranosyl-(1→2)-<em>β</em>-D-glucopyranoside (<strong>1</strong>) and 3-methoxyacetophenone-4-<em>O</em>-<em>β</em>-D-(5′′-<em>O</em>-<em>p</em>-hydroxybenzoyl)-apiofuranosyl-(1→2)-<em>β</em>-D-glucopyranoside (<strong>2</strong>), along with one known analogue (<strong>3</strong>), were isolated from the ethyl acetate extract of <em>Cyathula officinalis</em>. Extensive spectroscopic analyses and chemical methods determined their structures and absolute configurations. The relaxant effects of the isolated compounds on uterine contractions induced by oxytocin were investigated using a rat uterine smooth muscle contraction model. The results indicated that both compounds <strong>1</strong> and <strong>2</strong> had inhibitory effects on oxytocin-induced uterine smooth muscle contraction.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 100-104"},"PeriodicalIF":1.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical constituents of Himantormia lugubris collected from Antarctica and their PTP1B and α-glucosidase inhibitory activities","authors":"Manh Tuan Ha ,&nbsp;Thi Thanh Le ,&nbsp;Da Yeong Lee ,&nbsp;Chung Sub Kim ,&nbsp;Ui Joung Youn ,&nbsp;Sang Hee Kim ,&nbsp;Jeong Ah Kim ,&nbsp;Byung Sun Min","doi":"10.1016/j.phytol.2025.02.009","DOIUrl":"10.1016/j.phytol.2025.02.009","url":null,"abstract":"<div><div>A phytochemical investigation of an Antarctic endemic species [<em>Himantormia lugubris</em> (Hue) M. Lamb] led to the isolation and structural elucidation of three new compounds including one lanostane-type triterpenoid (<strong>1</strong>, himanlugubrol A), one ergostane-type sterol (<strong>2</strong>, himanlugubrol B), one benzyl orsellinate derivative (<strong>3</strong>, himanlugubrin A), along with ten known compounds (<strong>4</strong>−<strong>13</strong>). The chemical structures of new compounds were determined using diverse NMR techniques, HRESIMS data analysis, and computational approaches supported by advanced statistics (DP4+). The anti-diabetic potential of all isolated compounds was investigated by evaluating their inhibitory effects on PTP1B and α-glucosidase enzymes. As a result, compound <strong>3</strong> moderately inhibited PTP1B with an IC₅₀ value of 43.86 µM and significantly inhibited α-glucosidase (IC₅₀ = 73.46 µM) in comparison to the positive controls, ursolic acid (IC₅₀ = 5.92 µM) and acarbose (IC₅₀ = 210.11 µM), respectively. Enzyme kinetic analysis revealed that compound <strong>3</strong> demonstrated noncompetitive inhibition of PTP1B and mixed-type inhibition of α-glucosidase. Additionally, molecular docking results supported these in vitro findings, showing that compound <strong>3</strong> had strong binding affinities for the active sites of both PTP1B and α-glucosidase, indicated by the key H-bond and van der Waals interactions and negative binding energies.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 91-99"},"PeriodicalIF":1.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sargafusal, a rare norsesquiterpenoid from the edible brown alga Sargassum fusiforme","authors":"Junzhi Pan ,&nbsp;Guohao Hu ,&nbsp;Feijing Lv ,&nbsp;Zhongyang Shi ,&nbsp;Wuming Dong ,&nbsp;Yu Qi ,&nbsp;Chaojie Wang ,&nbsp;Haibing Tong ,&nbsp;Fangjian Huang ,&nbsp;Pengcheng Yan","doi":"10.1016/j.phytol.2025.02.008","DOIUrl":"10.1016/j.phytol.2025.02.008","url":null,"abstract":"<div><div>A previously undescribed C₁₄-norsesquiterpenoid aldehyde with a hexahydrobenzofuran scaffold, sargafusal (<strong>1</strong>), was isolated from an EtOAc-soluble extract of the edible brown alga <em>Sargassum fusiforme</em>, together with three known norisoprenoids (<strong>2</strong>–<strong>4</strong>). The chemical structure of sargafusal (<strong>1</strong>) was established by extensive spectroscopic analyses, including 1D and 2D NMR, ECD and DFT-calculated ECD, and HR-ESI-MS data. Sargafusal (<strong>1</strong>) represents the first C₁₄-norsesquiterpenoid aldehyde from the family Sargassaceae<em>.</em> Compounds <strong>1</strong>, <strong>3</strong>, and <strong>4</strong> showed potent antioxidant neuroprotective effects against H₂O₂-induced oxidative damage in neuron-like PC12 cells. This study provides new insight into the drug discovery efforts on the promising neuroprotective lead compounds from marine algae resource.</div></div>","PeriodicalId":20408,"journal":{"name":"Phytochemistry Letters","volume":"66 ","pages":"Pages 86-90"},"PeriodicalIF":1.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}