Preparative Biochemistry and Biotechnology最新文献_第8页

Enhanced poly(L-malic acid) production from pretreated cane molasses by Aureobasidium pullulans in fed-batch fermentation 普鲁兰小黑霉分批补料发酵预处理甘蔗糖蜜提高聚l -苹果酸产量

Preparative Biochemistry and Biotechnology Pub Date : 2016-02-01 DOI: 10.1080/10826068.2015.1135464

Jun Xia, Jiaxing Xu, Lei Hu, Xiaoyan Liu

{"title":"Enhanced poly(L-malic acid) production from pretreated cane molasses by Aureobasidium pullulans in fed-batch fermentation","authors":"Jun Xia, Jiaxing Xu, Lei Hu, Xiaoyan Liu","doi":"10.1080/10826068.2015.1135464","DOIUrl":"https://doi.org/10.1080/10826068.2015.1135464","url":null,"abstract":"ABSTRACT Poly(L-malic acid) (PMA) is a natural polyester with many attractive properties for biomedical application. However, the cost of PMA production is high when glucose is used as a carbon source. To solve this problem, cane molasses as a low-cost feedstock was applied for the production of PMA. Six pretreatment methods were applied to cane molasses before fermentation. Pretreatment with combined tricalcium phosphate, potassium ferrocyanide, and sulfuric acid (TPFSA) removed significant amounts of metal ions from cane molasses. The PMA concentration increased from 5.4 g/L (untreated molasses) to 36.9 g/L (TPFSA-pretreated molasses) after fermentation in shake flasks. A fed-batch fermentation strategy was then developed. In this method, TPFSA-pretreated cane molasses solution was continuously fed into the fermentor to maintain the total sugar concentration at 20 g/L. This technique generated approximately 95.4 g/L PMA with a productivity of 0.57 g/L/hr. The present study indicated that fed-batch fermentation using pretreated cane molasses is a feasible technique for producing high amounts of PMA.","PeriodicalId":20393,"journal":{"name":"Preparative Biochemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86491401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Trehalose phosphate synthase overexpression in Parachlorella kessleri improves growth and photosynthetic performance under high light conditions 海藻糖磷酸合成酶在高光照条件下的过度表达提高了疏伞藻的生长和光合性能

Preparative Biochemistry and Biotechnology Pub Date : 2016-02-01 DOI: 10.1080/10826068.2015.1135465

J. Rathod, G. Prakash, Chaitali Vira, A. Lali

引用次数: 13

Optimization of a recombinant human growth hormone purification process using quality by design 利用质量设计优化重组人生长激素纯化工艺

Preparative Biochemistry and Biotechnology Pub Date : 2016-02-01 DOI: 10.1080/10826068.2015.1135467

Carolina Ortiz-Enriquez, A. J. Romero-Díaz, A. V. Hernández-Moreno, H. F. Cueto-Rojas, M. Miranda-Hernández, C. López-Morales, N. O. Pérez, Rodolfo Salazar-Ceballos, Norberto Cruz-García, L. F. Flores-Ortiz, E. Medina-Rivero

{"title":"Optimization of a recombinant human growth hormone purification process using quality by design","authors":"Carolina Ortiz-Enriquez, A. J. Romero-Díaz, A. V. Hernández-Moreno, H. F. Cueto-Rojas, M. Miranda-Hernández, C. López-Morales, N. O. Pérez, Rodolfo Salazar-Ceballos, Norberto Cruz-García, L. F. Flores-Ortiz, E. Medina-Rivero","doi":"10.1080/10826068.2015.1135467","DOIUrl":"https://doi.org/10.1080/10826068.2015.1135467","url":null,"abstract":"ABSTRACT This work describes a strategy to optimize a downstream processing of a recombinant human growth hormone (rhGH) by incorporating a quality by design approach toward meeting higher quality specifications. The optimized process minimized the presence of impurities and degradation by-products during manufacturing by the establishment of in-process controls. Capillary zone electrophoresis, reverse phase, and size-exclusion chromatographies were used as analytical techniques to establish new critical process parameters for the solubilization, capture, and intermediate purification steps aiming to maintain rhGH quality by complying with pharmacopeial specifications. The results indicated that the implemented improvements in the process allowed the optimization of the specific recovery and purification of rhGH without compromising its quality. In addition, this optimization facilitated the stringent removal of the remaining impurities in further polishing stages, as demonstrated by the analysis of the obtained active pharmaceutical ingredient.","PeriodicalId":20393,"journal":{"name":"Preparative Biochemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83246238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cryoprotective properties and preliminary characterization of exopolysaccharide (P-Arcpo 15) produced by the Arctic bacterium Pseudoalteromonas elyakovii Arcpo 15 北极细菌pseudalteromonas elyakovii Arcpo 15产生的胞外多糖(P-Arcpo 15)的低温保护特性及初步表征

Preparative Biochemistry and Biotechnology Pub Date : 2016-01-25 DOI: 10.1080/10826068.2015.1015568

Sung Jin Kim, Byung-Gee Kim, H. Park, J. Yim

{"title":"Cryoprotective properties and preliminary characterization of exopolysaccharide (P-Arcpo 15) produced by the Arctic bacterium Pseudoalteromonas elyakovii Arcpo 15","authors":"Sung Jin Kim, Byung-Gee Kim, H. Park, J. Yim","doi":"10.1080/10826068.2015.1015568","DOIUrl":"https://doi.org/10.1080/10826068.2015.1015568","url":null,"abstract":"ABSTRACT Twenty-two bacterial strains that secrete exopolysaccharides (EPS) were isolated from marine samples obtained from the Chukchi Sea in the Arctic Ocean; of these, seven strains were found to be capable of producing cryoprotective EPS. The ArcPo 15 strain was isolated based on its ability to secrete large amounts of EPS, and was identified as Pseudoalteromonas elyakovii based on 16S rDNA analysis. The EPS, P-ArcPo 15, was purified by protease treatment and gel filtration chromatography. The purified EPS (P-ArcPo 15) had a molecular mass of 1.7 × 107 Da, and its infrared spectrum showed absorption bands of hydroxyl and carboxyl groups. The principal sugar components of P-ArcPo 15 were determined to be mannose and galacturonic acid, in the ratio of 3.3:1.0. The cryoprotective properties of P-ArcPo 15 were characterized by an Escherichia coli viability test. In the presence of 0.5% (w/v) EPS, the survival percentage of E. coli cells was as high as 94.19 ± 7.81% over five repeated freeze–thaw cycles. These biochemical characteristics suggest that the EPS P-ArcPo 15 may be useful in the development of cryoprotectants for biotechnological purposes, and we therefore assessed the utility of this novel cryoprotective EPS.","PeriodicalId":20393,"journal":{"name":"Preparative Biochemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90360135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Characterization of β-N-acetylglucosaminidase from a marine Pseudoalteromonas sp. for application in N-acetyl-glucosamine production 海洋假交替单胞菌sp. β- n -乙酰氨基葡萄糖酶在n -乙酰氨基葡萄糖生产中的应用

Preparative Biochemistry and Biotechnology Pub Date : 2016-01-21 DOI: 10.1080/10826068.2015.1135459

H. Park, J. Yim, Hyunro Park, Dockyu Kim

{"title":"Characterization of β-N-acetylglucosaminidase from a marine Pseudoalteromonas sp. for application in N-acetyl-glucosamine production","authors":"H. Park, J. Yim, Hyunro Park, Dockyu Kim","doi":"10.1080/10826068.2015.1135459","DOIUrl":"https://doi.org/10.1080/10826068.2015.1135459","url":null,"abstract":"ABSTRACT The psychrotolerant Pseudoalteromonas issachenkonii PAMC 22718 was isolated for its high exo-acting chitinase activity in the Kara Sea, Arctic. An exo-acting chitinase (W-Chi22718) was homogeneously purified from the culture supernatant of PAMC 22718, the molecular weight of which was estimated to be approximately 112 kDa. Due to its β-N-acetylglucosaminidase activity, W-Chi22718 was able to produce N-acetyl-D-glucosamine (GlcNAc) monomers from chitin oligosaccharide substrates. W-Chi22718 displayed chitinase activity from 0 to 37°C (optimal temperature of 30°C) and maintained activity from pH 6.0 to 9.0 (optimal pH of 7.6). W-Chi22718 exhibited a relative activity of 13 and 35% of maximal activity at 0 and 10°C, respectively, which is comparable to the activities of previously characterized, cold-adapted bacterial chitinases. W-Chi22718 activity was enhanced by K+, Ca2+, and Fe2+, but completely inhibited by Cu2+ and SDS. We found that W-Chi22718 can produce much more (GlcNAcs) from colloidal chitin, working together with previously characterized cold-active endochitinase W-Chi21702. Genome sequencing revealed that the corresponding gene (chi22718_IV) was 2,856 bp encoding a 951 amino acid protein with a calculated molecular weight of approximately 102 kDa.","PeriodicalId":20393,"journal":{"name":"Preparative Biochemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89510911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Optimization of the extraction of phenolic compounds from Cyclosorus extensa with solvents of varying polarities 不同极性溶剂萃取环花楸中酚类化合物的优化

Preparative Biochemistry and Biotechnology Pub Date : 2016-01-21 DOI: 10.1080/10826068.2015.1135457

A. Das, Prerna Khawas, Dibyakanta Seth, T. Miyaji, S. C. Deka

{"title":"Optimization of the extraction of phenolic compounds from Cyclosorus extensa with solvents of varying polarities","authors":"A. Das, Prerna Khawas, Dibyakanta Seth, T. Miyaji, S. C. Deka","doi":"10.1080/10826068.2015.1135457","DOIUrl":"https://doi.org/10.1080/10826068.2015.1135457","url":null,"abstract":"ABSTRACT The leaves of Cyclosorus extensa are used in the preparation of rice beer in Assam, India. The optimal conditions of time and temperature of fermentation for extraction of bioactive compounds from the dried leaves were obtained using response surface methodology. The central composite rotatable design was used and 13 experimental runs based on two-factor-five-level design were generated and performed for each of the solvents. The independent variables were extraction time (12 and 48 h) and temperature (25 and 55°C). The responses studied were total polyphenol content, radical scavenging activity, antibacterial activity, and antifungal activity. The analysis of variance of the test data was performed and the sequential sum of squares, F-value, R2, and adjusted R2 were deduced. The predicted models for all the response variables were adequately fitted to the observed experimental data (p ≤ 0.001). The maximum extraction of bioactive compounds under the optimum conditions of extraction temperature and time for hexane, ethyl acetate, methanol, and distilled water were found to be 25°C for 29.43 h, 28.28°C for 41.27 h, 43.95°C for 29.61 h, and 55.00°C for 48.00 h, respectively. It was also observed that the solubility of the polyphenols was higher in methanol, followed by ethyl acetate, and the highest antibacterial activity against Escherichia coli was shown by the ethyl acetate extracts.","PeriodicalId":20393,"journal":{"name":"Preparative Biochemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75890269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Partial purification and characterization of bacteriocin produced by Enterococcus faecalis DU10 and its probiotic attributes 粪肠球菌DU10产菌素的部分纯化、鉴定及其益生菌特性

Preparative Biochemistry and Biotechnology Pub Date : 2016-01-19 DOI: 10.1080/10826068.2015.1135451

Venkatesh Perumal, Ayyanna Repally, Ankaiah Dasari, Arul Venkatesan

{"title":"Partial purification and characterization of bacteriocin produced by Enterococcus faecalis DU10 and its probiotic attributes","authors":"Venkatesh Perumal, Ayyanna Repally, Ankaiah Dasari, Arul Venkatesan","doi":"10.1080/10826068.2015.1135451","DOIUrl":"https://doi.org/10.1080/10826068.2015.1135451","url":null,"abstract":"ABSTRACT A novel bacteriocin produced by avian duck isolated lactic acid bacterium Enterococcus faecalis DU10 was isolated. This bacteriocin showed a broad spectrum of antibacterial activity against important food-borne pathogens and was purified by size exclusion chromatography followed by reverse-phase high-performance liquid chromatography in a C-18 column. Tricine–SDS PAGE revealed the presence of a band with an estimated molecular mass of 6.3 kDa. The zymogram clearly linked the antimicrobial activity with this band. This result was further confirmed by mass-assisted laser desorption ionization time-of-flight mass spectrometry, since a sharp peak corresponding to 6.313 kDa was detected and the functional groups were revealed by Fourier transform infrared spectroscopy. Bacteriocin DU10 activity was found sensitive to proteinase-K and pepsin and partially affected by trypsin and α-chymotrypsin. The activity of bacteriocin DU10 was partially resistant to heat treatments ranging from 30 to 90°C for 30 min. It also withstood a treatment at 121°C for 10 min. Cytotoxicity of bacteriocin DU10 by methyl-thiazolyl-diphenyl-tetrazolium bromide assay showed that the viability of HT-29 and HeLa cells decreased 60 ± 0.7% and 43 ± 4.8%, respectively, in the presence of 3,200 AU/mL of bacteriocin. The strain withstood 0.3% w/v of bile oxgall and pH 2 affected the bacterial growth between 2 and 4 hr of incubation. Adhesion properties examined with HT-29 cell line showed 69.85% initial population of strain E. faecalis DU10, which was found to be strongly adhered to this cell line. These results conclude bacteriocin DU10 may be used as a potential biopreservative and E. faecalis DU10 may be used as a potential probiont to control Salmonella infections.","PeriodicalId":20393,"journal":{"name":"Preparative Biochemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88631294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Purification and characterization of a soluble glycoprotein from garlic (Allium sativum) and its in vitro bioactivity 大蒜可溶性糖蛋白的纯化、鉴定及其体外生物活性

Preparative Biochemistry and Biotechnology Pub Date : 2016-01-19 DOI: 10.1080/10826068.2015.1135454

Yan Wang, Tingting Zou, Minghui Xiang, Chenzhong Jin, Xuejiao Zhang, Yong Chen, Q. Jiang, Yihong Hu

引用次数: 8

Use of elephant grass (Pennisetum purpureum) acid hydrolysate for microbial oil production by Trichosporon cutaneum 利用象草(Pennisetum purpureum)酸水解物进行皮肤毛孢丝虫油的微生物生产

Preparative Biochemistry and Biotechnology Pub Date : 2016-01-15 DOI: 10.1080/10826068.2015.1135453

Xue-fang Chen, Chao Huang, L. Xiong, Bo Wang, Gao-xiang Qi, Xiaoqing Lin, Can Wang, Xindong Chen

引用次数: 13

A novel technology coupling extraction and foam fractionation for separating the total saponins from Achyranthes bidentata 采用耦合萃取-泡沫分馏分离牛膝总皂苷的新工艺

Preparative Biochemistry and Biotechnology Pub Date : 2016-01-15 DOI: 10.1080/10826068.2015.1135448

Linlin Ding, Yanji Wang, Zhaoliang Wu, Wei Liu, Rui Li, Yanyan Wang

引用次数: 6