Optimization of the extraction of phenolic compounds from Cyclosorus extensa with solvents of varying polarities

A. Das, Prerna Khawas, Dibyakanta Seth, T. Miyaji, S. C. Deka
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引用次数: 1

Abstract

ABSTRACT The leaves of Cyclosorus extensa are used in the preparation of rice beer in Assam, India. The optimal conditions of time and temperature of fermentation for extraction of bioactive compounds from the dried leaves were obtained using response surface methodology. The central composite rotatable design was used and 13 experimental runs based on two-factor-five-level design were generated and performed for each of the solvents. The independent variables were extraction time (12 and 48 h) and temperature (25 and 55°C). The responses studied were total polyphenol content, radical scavenging activity, antibacterial activity, and antifungal activity. The analysis of variance of the test data was performed and the sequential sum of squares, F-value, R2, and adjusted R2 were deduced. The predicted models for all the response variables were adequately fitted to the observed experimental data (p ≤ 0.001). The maximum extraction of bioactive compounds under the optimum conditions of extraction temperature and time for hexane, ethyl acetate, methanol, and distilled water were found to be 25°C for 29.43 h, 28.28°C for 41.27 h, 43.95°C for 29.61 h, and 55.00°C for 48.00 h, respectively. It was also observed that the solubility of the polyphenols was higher in methanol, followed by ethyl acetate, and the highest antibacterial activity against Escherichia coli was shown by the ethyl acetate extracts.
不同极性溶剂萃取环花楸中酚类化合物的优化
摘要:印度阿萨姆邦的米啤酒中使用了环sorus extensa的叶子。利用响应面法,确定了从干叶中提取生物活性物质的最佳发酵时间和发酵温度。采用中心复合旋转设计,基于两因素五水平设计,对每种溶剂进行了13次实验运行。自变量为提取时间(12和48 h)和温度(25和55℃)。研究了总多酚含量、自由基清除活性、抗菌活性和抗真菌活性。对试验数据进行方差分析,推导出序列平方和、f值、R2和调整后R2。所有响应变量的预测模型都与观察到的实验数据充分拟合(p≤0.001)。正己烷、乙酸乙酯、甲醇和蒸馏水的最佳提取温度和时间分别为25°C 29.43 h、28.28°C 41.27 h、43.95°C 29.61 h和55.00°C 48.00 h。多酚在甲醇中的溶解度最高,其次是乙酸乙酯,乙酸乙酯提取物对大肠杆菌的抑菌活性最高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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