A novel technology coupling extraction and foam fractionation for separating the total saponins from Achyranthes bidentata

Preparative Biochemistry and Biotechnology Pub Date : 2016-01-15 DOI:10.1080/10826068.2015.1135448

Linlin Ding, Yanji Wang, Zhaoliang Wu, Wei Liu, Rui Li, Yanyan Wang

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引用次数: 6

Abstract

ABSTRACT A novel technology coupling extraction and foam fractionation was developed for separating the total saponins from Achyranthes bidentata. In the developed technology, the powder of A. bidentata was loaded in a nylon filter cloth pocket with bore diameter of 180 µm. The pocket was fixed in the bulk liquid phase for continuously releasing saponins. Under the optimal conditions, the concentration and the extraction rate of the total saponins in the foamate by the developed technology were 73.5% and 416.2% higher than those by the traditional technology, respectively. The foamates obtained by the traditional technology and the developed technology were analyzed by ultraperformance liquid chromatography–mass spectrometry to determine their ingredients, and the results appeared that the developed technology exhibited a better performance for separating saponins than the traditional technology. The study is expected to develop a novel technology for cost effectively separating plant-derived materials with surface activity.

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采用耦合萃取-泡沫分馏分离牛膝总皂苷的新工艺

摘要建立了一种耦合萃取-泡沫分馏分离牛膝总皂苷的新工艺。在开发的工艺中，将刺竹粉末装入直径为180µm的尼龙滤布口袋中。将囊袋固定在散装液相中，连续释放皂苷。在最优条件下，采用该工艺提取乙酸乙酯中总皂苷的浓度和提取率分别比传统工艺高73.5%和416.2%。采用超高效液相色谱-质谱联用技术对传统工艺和新工艺制备的泡沫物进行成分分析，结果表明，新工艺对皂苷的分离效果优于传统工艺。该研究有望开发一种新的技术，以经济有效地分离具有表面活性的植物源材料。

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Preparative Biochemistry and Biotechnology

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