Bioprocess optimization for production of thermoalkali-stable protease from Bacillus subtilis K-1 under solid-state fermentation

Satbir Singh, B. K. Bajaj
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引用次数: 32

Abstract

ABSTRACT Cost-effective production of proteases, which are robust enough to function under harsh process conditions, is always sought after due to their wide industrial application spectra. Solid-state production of enzymes using agro-industrial wastes as substrates is an environment-friendly approach, and it has several advantages such as high productivity, cost-effectiveness, being less labor-intensive, and less effluent production, among others. In the current study, different agro-wastes were employed for thermoalkali-stable protease production from Bacillus subtilis K-1 under solid-state fermentation. Agricultural residues such as cotton seed cake supported maximum protease production (728 U ml−1), which was followed by gram husk (714 U ml−1), mustard cake (680 U ml−1), and soybean meal (653 U ml−1). Plackett–Burman design of experiment showed that peptone, moisture content, temperature, phosphates, and inoculum size were the significant variables that influenced the protease production. Furthermore, statistical optimization of three variables, namely peptone, moisture content, and incubation temperature, by response surface methodology resulted in 40% enhanced protease production as compared to that under unoptimized conditions (from initial 728 to 1020 U ml−1). Thus, solid-state fermentation coupled with design of experiment tools represents a cost-effective strategy for production of industrial enzymes.
枯草芽孢杆菌K-1固态发酵产热碱稳定蛋白酶的生物工艺优化
由于蛋白酶具有广泛的工业应用光谱,在苛刻的工艺条件下具有足够的鲁棒性,因此具有成本效益的蛋白酶生产一直受到追捧。利用农业工业废物作为底物的固体酶生产是一种环境友好的方法,它具有若干优点,例如生产率高、成本效益高、劳动密集程度低、废水产生少等。本研究利用不同的农业废弃物对枯草芽孢杆菌K-1进行固态发酵产热碱稳定蛋白酶。农业残留物如棉籽饼支持最大的蛋白酶产量(728 U ml−1),其次是克壳(714 U ml−1),芥菜饼(680 U ml−1)和豆粕(653 U ml−1)。Plackett-Burman实验设计表明,蛋白胨、水分含量、温度、磷酸盐和接种量是影响蛋白酶产量的重要变量。此外,通过响应面法统计优化三个变量,即蛋白胨、水分含量和孵育温度,与未优化条件(从初始的728到1020 U ml−1)相比,蛋白酶产量提高了40%。因此,固态发酵结合实验工具的设计代表了工业酶生产的成本效益策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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