Platelets最新文献_第2页

Light transmission aggregometry for platelet function testing: position paper on current recommendations and French proposals for accreditation. 用于血小板功能检测的透光聚集测定法：关于当前建议和法国认证提案的立场文件。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-11-18 DOI: 10.1080/09537104.2024.2427745

Alain Stépanian, Florence Fischer, Claire Flaujac, Valérie Eschwège, Céline Delassasseigne, Léna Leflem, Frédéric Loridon, Sophie Voisin, Dominique Lasne

{"title":"Light transmission aggregometry for platelet function testing: position paper on current recommendations and French proposals for accreditation.","authors":"Alain Stépanian, Florence Fischer, Claire Flaujac, Valérie Eschwège, Céline Delassasseigne, Léna Leflem, Frédéric Loridon, Sophie Voisin, Dominique Lasne","doi":"10.1080/09537104.2024.2427745","DOIUrl":"10.1080/09537104.2024.2427745","url":null,"abstract":"Light transmission aggregometry (LTA) is a method used to investigate platelet functions in platelet-rich plasma (PRP), notably when screening for platelet disorders. Various national guidelines and recommendations help in setting up the LTA test in specialized laboratories. However, due to the nature of the sample matrix and its subsequent specificities, more accurate positions are needed to achieve LTA accreditation according to the standard NF EN ISO 15 189. We reviewed guidelines and recommendations as they can be useful in the accreditation process, and we conducted a survey on LTA practice among members of the Société Française de Thrombose et d'Hémostase (SFTH) in 2021. We formulated 28 proposals, which have been approved by vote within the SFTH. All aspects to take into consideration for the proper conduct of LTA assays and their accreditation have been covered. Notably, preanalytical, analytical and postanalytical aspects are depicted, including blood sampling, PRP preparation, instruments, agonists, performance assessment, personnel training and data interpretation. This document, essentially representing a French position paper on the current recommendations and subsequent proposals for LTA accreditation, might prove useful also outside France for relevant laboratories and auditors involved in LTA accreditation.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2427745"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Appraising non-linear association between pre-diagnostic platelet counts and cancer survival outcomes: observational and genetic analysis. 评估诊断前血小板计数与癌症生存结果之间的非线性关联：观察和遗传分析。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-07-29 DOI: 10.1080/09537104.2024.2379815

Changtao Li, Junhua Chen, Deqian Han, Chi Shu, Jun Huang, Linru Wei, Haoran Luo, Qingbin Wu, Xin Chen, Yazhou He, Yanhong Zhou

{"title":"Appraising non-linear association between pre-diagnostic platelet counts and cancer survival outcomes: observational and genetic analysis.","authors":"Changtao Li, Junhua Chen, Deqian Han, Chi Shu, Jun Huang, Linru Wei, Haoran Luo, Qingbin Wu, Xin Chen, Yazhou He, Yanhong Zhou","doi":"10.1080/09537104.2024.2379815","DOIUrl":"https://doi.org/10.1080/09537104.2024.2379815","url":null,"abstract":"Previous studies have reported inconsistent associations between platelet count (PLT) and cancer survival. However, whether there is linear causal effect merits in-depth investigations. We conducted a cohort study using the UK Biobank and a two-sample Mendelian randomization (MR) analysis. PLT levels were measured prior to cancer diagnosis. We adopted overall survival (OS) as the primary outcome. Cox models were utilized to estimate the effects of PLTs on survival outcomes at multiple lag times for cancer diagnosis. We employed 34 genetic variants as PLT proxies for MR analysis. Linear and non-linear effects were modeled. Prognostic effects of gene expression harboring the instrumental variants were also investigated. A total of 65 471 cancer patients were included. We identified a significant association between elevated PLTs (per 100 × 109/L) and inferior OS (HR: 1.07; 95% CI: 1.04-1.10; p < .001). Similar significant associations were observed for several cancer types. We further observed a U-shaped relationship between PLTs and cancer survival (p < .001). Our MR analysis found null evidence to support a causal association between PLTs and overall cancer survival (HR: 1.000; 95% CI: 0.998-1.001; p = .678), although non-linear MR analysis unveiled a potential greater detrimental effect at lower PLT range. Expression of eleven PLT-related genes were associated with cancer survival. Early detection of escalated PLTs indicated possible occult cancer development and inferior subsequent survival outcomes. The observed associations could potentially be non-linear. However, PLT is less likely to be a promising therapeutic target.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2379815"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the morphine-platelet activity association in patients with ST-elevation myocardial infarction undergoing primary percutaneous coronary intervention. st段抬高型心肌梗死患者经皮冠状动脉介入治疗中吗啡-血小板活性的相关性研究。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-12-06 DOI: 10.1080/09537104.2024.2434225

Menikae K Heenkenda, Erik Träff, Tomas L Lindahl, Dimitrios Venetsanos, Joakim Alfredsson

{"title":"Exploring the morphine-platelet activity association in patients with ST-elevation myocardial infarction undergoing primary percutaneous coronary intervention.","authors":"Menikae K Heenkenda, Erik Träff, Tomas L Lindahl, Dimitrios Venetsanos, Joakim Alfredsson","doi":"10.1080/09537104.2024.2434225","DOIUrl":"https://doi.org/10.1080/09537104.2024.2434225","url":null,"abstract":"ST-segment elevation myocardial infarction (STEMI) is usually caused by a ruptured atherosclerotic plaque, with subsequent thrombus formation. Platelet inhibition and primary percutaneous coronary intervention (PCI) are essential treatments. Morphine, used to relieve pain and anxiety in STEMI patients, delays the onset of P2Y12 inhibitors. This study aimed to further explore the association between platelet activity and morphine treatment in patients with STEMI. In this sub-study of the VALIDATE-SWEDHEART trial, 89 STEMI patients treated with ticagrelor, and primary PCI were included. Platelet aggregation and biomarkers of platelet activity, coagulation, and inflammation (sP-selectin, thrombin-antithrombin complexes, prothrombin fragments 1 + 2, CD40L, CRP, beta-thromboglobulin, and pentraxin3) were assessed at three time points: before, one, and twelve hours after PCI. Of the 89 patients, 40 received morphine before hospital arrival. There were no significant differences in age, sex, medical history, or coronary disease extent. One hour after PCI, ADP-induced (36 vs 61, p < .001), arachidonic acid-induced (20 vs 36, p = .003), collagen-induced (48 vs 60, p = .03) aggregation, and the proportion of high on-treatment ADP-induced platelet reactivity (27% vs 60%, p = .001) were significantly higher in morphine-treated patients. No significant differences were found before or 12 hours after PCI. No significant differences in platelet activity biomarkers were observed. Morphine increased platelet aggregation in STEMI patients but did not affect biomarkers.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2434225"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The efficacy of platelet rich plasma on women with poor ovarian response: a systematic review and meta-analysis. 富血小板血浆对卵巢反应不良妇女的疗效：系统综述和荟萃分析。

IF 3.3 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-01-12 DOI: 10.1080/09537104.2023.2292612

Lingling Wu, Fenfang Su, Peixin Luo, Qingqing Dong, Mengni Ma, Guangyong Ye

{"title":"The efficacy of platelet rich plasma on women with poor ovarian response: a systematic review and meta-analysis.","authors":"Lingling Wu, Fenfang Su, Peixin Luo, Qingqing Dong, Mengni Ma, Guangyong Ye","doi":"10.1080/09537104.2023.2292612","DOIUrl":"10.1080/09537104.2023.2292612","url":null,"abstract":"Background: Platelet-rich plasma (PRP) is a therapeutic approach that is gaining attention for its potential in the treatment of poor ovarian response. This meta-analysis aimed to systematically review and analyze clinical studies to evaluate the impact of PRP on poor responders undergoing ovarian stimulation for IVF.Methods: A comprehensive search was conducted in electronic databases, including PubMed, Embase, Scopus, Web of Science, and the Cochrane Library to identify relevant studies published in English. The pooled data, such as pregnancy outcome, number of MII oocytes, number of transferable embryos, and ovarian reserve markers were analyzed using R version 4.2.3.Results: A total of 10 trials were enrolled in the present meta-analysis. Following PRP treatment, live birth rate was found to be 16.6% (95% CI 8.8%-26.1%), while clinical pregnancy rate was observed to be 25.4% (95% CI 13.1%-39.9%). PRP pretreatment resulted in a higher number of MII oocytes (MD 1.073, 95% CI 0.720 to 1.427), a higher number of embryos (MD 0.946, 95% CI 0.569 to 1.323), a higher antral follicle count (MD 1.117; 95% CI 0.689 to 1.544), and the change of hormone levels.Conclusions: Among the studies evaluated in this review, PRP showed promising results in poor responder. Further research is required to clarify the potential role of PRP in female reproductive health.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2292612"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139425387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case of acquired transient bleeding diathesis associated with acquired platelet storage pool deficiency and defective thromboxane A2 production. 一例与获得性血小板储存池缺乏症和血栓素 A2 生成缺陷有关的获得性一过性出血综合症。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-06-04 DOI: 10.1080/09537104.2024.2358241

Mariangela Scavone, Bianca Clerici, Eti Alessandra Femia, Claudia Ghali, Antonella Fioretti, Elena Bossi, Marco Cattaneo, Gian Marco Podda

{"title":"A case of acquired transient bleeding diathesis associated with acquired platelet storage pool deficiency and defective thromboxane A2 production.","authors":"Mariangela Scavone, Bianca Clerici, Eti Alessandra Femia, Claudia Ghali, Antonella Fioretti, Elena Bossi, Marco Cattaneo, Gian Marco Podda","doi":"10.1080/09537104.2024.2358241","DOIUrl":"10.1080/09537104.2024.2358241","url":null,"abstract":"Acquired disorders of platelet function are an underdiagnosed cause of bleeding tendency. A 14-year-old girl developed moderate mucocutaneous bleeding two weeks after a Mycoplasma pneumoniae infection successfully treated with clarithromycin. The patient was referred to us 7 months later for laboratory investigation of the persisting bleeding diathesis. The patient's personal and family histories were negative for bleeding disorders. Complete blood count, von Willebrand Factor levels and coagulation tests were normal; platelet aggregation, ATP secretion, δ-granules content and serum thromboxane B2 levels were defective. At follow-up visits, laboratory parameters and the bleeding diathesis progressively normalized within 2 years. The patient's condition is compatible with a diagnosis of acquired Storage Pool Deficiency (SPD), associated with defective thromboxane A2 production. To our knowledge, this is the first case of acquired, transient SPD with spontaneous remission. The pathogenic role of Mycoplasma pneumoniae infection or clarithromycin is possible, albeit uncertain.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2358241"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141238103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial and temporal characterization of cytoskeletal reorganizations in adherent platelets. 粘附血小板细胞骨架重组的空间和时间特征。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1080/09537104.2024.2422437

Clotilde Joubert, Alexei Grichine, Monika Dolega, Sophie Michallet, Florence Appaix, Isabelle Tardieux, Laurence Lafanechère, Karin Sadoul

{"title":"Spatial and temporal characterization of cytoskeletal reorganizations in adherent platelets.","authors":"Clotilde Joubert, Alexei Grichine, Monika Dolega, Sophie Michallet, Florence Appaix, Isabelle Tardieux, Laurence Lafanechère, Karin Sadoul","doi":"10.1080/09537104.2024.2422437","DOIUrl":"https://doi.org/10.1080/09537104.2024.2422437","url":null,"abstract":"The functional role of platelets is intricately linked to the dynamic organization of two main components of the cytoskeleton, microtubules and actin fibers. Throughout the phases of platelet activation, spreading, and retraction, both of these essential polymers undergo continuous and orchestrated reorganization. Our investigation of the dynamic cytoskeletal changes during these phases highlights a sequential remodeling of the actin cytoskeleton in adherent platelets from the formation of initial actin nodules through the development of stress fibers and a subsequent return to nodular structures. Concurrently, the marginal ring of microtubules, characteristic of resting platelets, undergoes a re-organization induced by marginal band extension and coiling toward the formation of star-like bundles of microtubules. Subsequently, these bundles are dispersed into individual microtubules, which are re-bundled at later stages before ring-like structures are formed again. These findings suggest a compelling tendency for both cytoskeletal components to revert to their original configurations. Notably, the early steps of platelet cytoskeleton reorganizations have previously been shown to be regulated by the signaling cascade triggered during platelet activation, which leads to an increase of cytosolic calcium concentrations. We show here that later steps are potentially regulated by a progressive decrease of intracellular calcium concentrations as platelets approach the end of their functional lifespan.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2422437"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced platelet sensitization is accompanied by increased expression of the transporter MRP4 and elevated plasma S1P levels in mild COVID-19 convalescents. 在轻度 COVID-19 康复者中，伴随着转运体 MRP4 表达的增加和血浆 S1P 水平的升高，血小板致敏性增强。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-10-30 DOI: 10.1080/09537104.2024.2413713

Céline Tolksdorf, Andrea Seidel, Christian Baume, Eileen Moritz, Karen Saljé, Kathrin Lehmann, Karsten Becker, Ulrike Garscha, Thomas Thiele, Edzard Schwedhelm, Mirjam von Lucadou, Mladen V Tzvetkov, Stefan Engeli, Gabriele Jedlitschky, Bernhard H Rauch

{"title":"Enhanced platelet sensitization is accompanied by increased expression of the transporter MRP4 and elevated plasma S1P levels in mild COVID-19 convalescents.","authors":"Céline Tolksdorf, Andrea Seidel, Christian Baume, Eileen Moritz, Karen Saljé, Kathrin Lehmann, Karsten Becker, Ulrike Garscha, Thomas Thiele, Edzard Schwedhelm, Mirjam von Lucadou, Mladen V Tzvetkov, Stefan Engeli, Gabriele Jedlitschky, Bernhard H Rauch","doi":"10.1080/09537104.2024.2413713","DOIUrl":"https://doi.org/10.1080/09537104.2024.2413713","url":null,"abstract":"Viral infections can lead to platelet activation and hemostatic complications. However, the extent to which platelet reactivity remains altered after convalescence, contributing to long-term health impairments as observed after COVID-19 is not yet fully understood. Therefore, we conducted a cohort study (DRKS00025217) to determine platelet function in individuals convalesced from mild COVID-19. Assays were performed ex vivo with blood from convalescents at 2-15 weeks and 6-10 months after convalescence, focusing on platelet aggregation, activation markers, and thrombin formation. In addition, two other potentially relevant factors for platelet function were examined: the immunomodulatory mediator sphingosine-1-phosphate (S1P) and the platelet expression of the transporter MRP4 (ABCC4). Our findings indicate that robust platelet functions, including platelet aggregation determined by light transmission aggregometry, and thrombin formation, were not altered in convalescents compared to matched control individuals. However, an elevation in subtle platelet activation markers, such as P-selectin surface expression and activation of glycoprotein IIb/IIIa, was observed 2-15 weeks after convalescence. This was accompanied by an increased expression of MRP4 in platelets and significantly elevated levels of S1P in platelet-poor plasma. Our findings suggest increased platelet sensitization and a pro-inflammatory state even after convalescence from mild COVID-19, pointing toward MRP4 and S1P as associated factors.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2413713"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding and following: a deep learning-based pipeline for tracking platelets during thrombus formation in vivo and ex vivo. 寻找和跟踪：基于深度学习的管道，用于在体内和体外血栓形成过程中跟踪血小板。

IF 3.3 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-05-09 DOI: 10.1080/09537104.2024.2344512

Abigail S McGovern, Pia Larsson, Volga Tarlac, Natasha Setiabakti, Leila Shabani Mashcool, Justin R Hamilton, Niklas Boknäs, Juan Nunez-Iglesias

{"title":"Finding and following: a deep learning-based pipeline for tracking platelets during thrombus formation in vivo and ex vivo.","authors":"Abigail S McGovern, Pia Larsson, Volga Tarlac, Natasha Setiabakti, Leila Shabani Mashcool, Justin R Hamilton, Niklas Boknäs, Juan Nunez-Iglesias","doi":"10.1080/09537104.2024.2344512","DOIUrl":"https://doi.org/10.1080/09537104.2024.2344512","url":null,"abstract":"The last decade has seen increasing use of advanced imaging techniques in platelet research. However, there has been a lag in the development of image analysis methods, leaving much of the information trapped in images. Herein, we present a robust analytical pipeline for finding and following individual platelets over time in growing thrombi. Our pipeline covers four steps: detection, tracking, estimation of tracking accuracy, and quantification of platelet metrics. We detect platelets using a deep learning network for image segmentation, which we validated with proofreading by multiple experts. We then track platelets using a standard particle tracking algorithm and validate the tracks with custom image sampling - essential when following platelets within a dense thrombus. We show that our pipeline is more accurate than previously described methods. To demonstrate the utility of our analytical platform, we use it to show that in vivo thrombus formation is much faster than that ex vivo. Furthermore, platelets in vivo exhibit less passive movement in the direction of blood flow. Our tools are free and open source and written in the popular and user-friendly Python programming language. They empower researchers to accurately find and follow platelets in fluorescence microscopy experiments.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2344512"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-life clinical practice in Spain in the setting of new drug availability for ITP treatment. A Delphi-based Spanish expert panel consensus. 在新药上市的背景下西班牙治疗 ITP 的实际临床实践。基于德尔菲法的西班牙专家小组共识。

IF 3.3 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-05-14 DOI: 10.1080/09537104.2024.2336104

Tomás José González-López, Abelardo Bárez, Ángel Bernardo-Gutiérrez, Silvia Bernat, Fernando Fernández-Fuertes, José María Guinea de Castro, Reyes Jiménez-Bárcenas, Isidro Jarque

{"title":"Real-life clinical practice in Spain in the setting of new drug availability for ITP treatment. A Delphi-based Spanish expert panel consensus.","authors":"Tomás José González-López, Abelardo Bárez, Ángel Bernardo-Gutiérrez, Silvia Bernat, Fernando Fernández-Fuertes, José María Guinea de Castro, Reyes Jiménez-Bárcenas, Isidro Jarque","doi":"10.1080/09537104.2024.2336104","DOIUrl":"https://doi.org/10.1080/09537104.2024.2336104","url":null,"abstract":"Immune thrombocytopenia (ITP) is a common autoimmune hematological disorder. Despite this, diagnosis is still challenging due to clinical heterogeneity and the lack of a specific diagnostic test. New findings in the pathology and the availability of new drugs have led to the development of different guidelines worldwide. In the present study, the Delphi methodology has been used to get a consensus on the management of adult patients with ITP in Spain and to help in decision-making. The Delphi questionnaire has been designed by a scientific ad hoc committee and has been divided into 13 topics, with a total of 127 items, covering the maximum possible scenarios for the management of ITP. As a result of the study, a total consensus of 81% has been reached. It is concluded that this Delphi consensus provides practical recommendations on topics related to diagnosis and management of ITP patients to help doctors to improve outcomes. Some aspects remain unclear, without consensus among the experts. Thus, more advances are needed to optimize ITP management.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2336104"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemin regulates platelet clearance in hemolytic disease by binding to GPIbα. 血红蛋白通过与 GPIbα 结合调节溶血性疾病中的血小板清除。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-07-29 DOI: 10.1080/09537104.2024.2383642

Man Zhao, Dongxin Peng, Yuxuan Li, Minwei He, Yulong Zhang, Qianqian Zhou, Sujing Sun, Ping Ma, Liping Lv, Xiaohui Wang, Linsheng Zhan

{"title":"Hemin regulates platelet clearance in hemolytic disease by binding to GPIbα.","authors":"Man Zhao, Dongxin Peng, Yuxuan Li, Minwei He, Yulong Zhang, Qianqian Zhou, Sujing Sun, Ping Ma, Liping Lv, Xiaohui Wang, Linsheng Zhan","doi":"10.1080/09537104.2024.2383642","DOIUrl":"10.1080/09537104.2024.2383642","url":null,"abstract":"Hemolysis is associated with thrombosis and vascular dysfunction, which are the pathological components of many diseases. Hemolytic products, including hemoglobin and hemin, activate platelets (PLT). Despite its activation, the effect of hemolysis on platelet clearance remains unclear, It is critical to maintain a normal platelet count and ensure that circulating platelets are functionally viable. In this study, we used hemin, a degradation product of hemoglobin, as a potent agonist to treat platelets and simulate changes in vivo in mice. Hemin treatment induced activation and morphological changes in platelets, including an increase in intracellular Ca2+ levels, phosphatidylserine (PS) exposure, and cytoskeletal rearrangement. Fewer hemin-treated platelets were cleared by macrophages in the liver after transfusion than untreated platelets. Hemin bound to glycoprotein Ibα (GPIbα), the surface receptor in hemin-induced platelet activation and aggregation. Furthermore, hemin decreased GPIbα desialylation, as evidenced by reduced Ricinus communis agglutinin I (RCA- I) binding, which likely extended the lifetime of such platelets in vivo. These data provided new insight into the mechanisms of GPIbα-mediated platelet activation and clearance in hemolytic disease.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2383642"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0