Platelets最新文献_第3页

Effect of aspirin dosage on oxidative stress and platelet reactivity in patients undergoing coronary artery bypass grafting (APRICOT): randomized controlled trial. 阿司匹林剂量对冠状动脉旁路移植术患者氧化应激和血小板反应性的影响：随机对照试验。

IF 2.5 3区医学

Platelets Pub Date : 2025-12-01 Epub Date: 2025-02-05 DOI: 10.1080/09537104.2025.2457415

Aleksandra Gąsecka, Rafał Kaczorowski, Katarzyna Pomykała, Tomasz Kucharski, Magdalena Gajewska, Dominika Siwik, Katarzyna Karoń, Maciej Małyszko, Jaromir Hunia, Jakub Michal Zimodro, Paweł Kowalczyk, Oliwia Zagrocka-Stendel, Małgorzata Dutkiewicz, Katarzyna Koziak, Ceren Eyileten, Marek Postuła, Mateusz Wondołkowski, Marcin Grabowski, Mariusz Kuśmierczyk, Radosław Wilimski

{"title":"Effect of aspirin dosage on oxidative stress and platelet reactivity in patients undergoing coronary artery bypass grafting (APRICOT): randomized controlled trial.","authors":"Aleksandra Gąsecka, Rafał Kaczorowski, Katarzyna Pomykała, Tomasz Kucharski, Magdalena Gajewska, Dominika Siwik, Katarzyna Karoń, Maciej Małyszko, Jaromir Hunia, Jakub Michal Zimodro, Paweł Kowalczyk, Oliwia Zagrocka-Stendel, Małgorzata Dutkiewicz, Katarzyna Koziak, Ceren Eyileten, Marek Postuła, Mateusz Wondołkowski, Marcin Grabowski, Mariusz Kuśmierczyk, Radosław Wilimski","doi":"10.1080/09537104.2025.2457415","DOIUrl":"10.1080/09537104.2025.2457415","url":null,"abstract":"Coronary artery bypass grafting (CABG) triggers oxidative stress and platelet activation. High acetylsalicylic acid (ASA) dose might mitigate the transient proinflammatory state. We compared the effect of three ASA dosages on post-CABG platelet reactivity, oxidative stress, and serum CD39 and CD73 levels. Thirty-six consecutive patients undergoing elective off-pump CABG, pre-treated with ASA 1 × 75 mg for ≥7 days, were randomized to continue the prior treatment regimen, switch to ASA 1 × 150 mg, or ASA 2 × 75 mg. Blood was collected on admission, 7 days, 1 month, and 3 months after CABG. Platelet reactivity was assessed using impedance aggregometry. Platelet oxidative stress was measured as platelet mitochondria extracellular oxygen consumption rate and oxidatively damaged whole-blood DNA cleavage. Serum CD39 and CD73 levels were determined using ELISA. Platelet reactivity and oxidative stress parameters were comparable in all groups. Patients treated with ASA 2 × 75 mg had higher CD39 levels at 7 days and 1 month (p = .049, p = .033), compared to the control group. ASA 2 × 75 mg was associated a beneficial effect on serum CD39 levels after off-pump CABG, without a significant effect on oxidative stress parameters.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"36 1","pages":"2457415"},"PeriodicalIF":2.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased circulating platelet-derived extracellular vesicles in severe COVID-19 disease. 严重 COVID-19 疾病中循环血小板衍生细胞外囊泡增加。

IF 3.3 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-02-21 DOI: 10.1080/09537104.2024.2313362

Tuukka Helin, Mari Palviainen, Marja Lemponen, Katariina Maaninka, Pia Siljander, Lotta Joutsi-Korhonen

{"title":"Increased circulating platelet-derived extracellular vesicles in severe COVID-19 disease.","authors":"Tuukka Helin, Mari Palviainen, Marja Lemponen, Katariina Maaninka, Pia Siljander, Lotta Joutsi-Korhonen","doi":"10.1080/09537104.2024.2313362","DOIUrl":"10.1080/09537104.2024.2313362","url":null,"abstract":"Coagulation disturbances are major contributors to COVID-19 pathogenicity, but limited data exist on the involvement of extracellular vesicles (EVs) and residual cells (RCs). Fifty hospitalized COVID-19 patients stratified by their D-dimer levels into high (>1.5 mg/L, n = 15) or low (≤1.5 mg/l, n = 35) and 10 healthy controls were assessed for medium-sized EVs (mEVs; 200-1000 nm) and large EVs/RCs (1000-4000 nm) by high sensitivity flow cytometry. EVs were analyzed for CD61, CD235a, CD45, and CD31, commonly used to detect platelets, red blood cells, leukocytes or endothelial cells, respectively, whilst phosphatidyl serine EVs/RCs were detected by lactadherin-binding implicating procoagulant catalytic surface. Small EV detection (sEVs; 50-200 nm) and CD41a (platelet integrin) colocalization with general EV markers CD9, CD63, and CD81 were performed by single particle interferometric reflectance imaging sensor. Patients with increased D-dimer exhibited the highest number of RCs and sEVs irrespective of cell origin (p < .05). Platelet activation, reflected by increased CD61+ and lactadherin+ mEV and RC levels, associated with coagulation disturbances. Patients with low D-dimer could be discriminated from controls by tetraspanin signatures of the CD41a+ sEVs, suggesting the changes in the circulating platelet sEV subpopulations may offer added prognostic value during COVID progression.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2313362"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139913275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light transmission aggregometry for platelet function testing: position paper on current recommendations and French proposals for accreditation. 用于血小板功能检测的透光聚集测定法：关于当前建议和法国认证提案的立场文件。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-11-18 DOI: 10.1080/09537104.2024.2427745

Alain Stépanian, Florence Fischer, Claire Flaujac, Valérie Eschwège, Céline Delassasseigne, Léna Leflem, Frédéric Loridon, Sophie Voisin, Dominique Lasne

{"title":"Light transmission aggregometry for platelet function testing: position paper on current recommendations and French proposals for accreditation.","authors":"Alain Stépanian, Florence Fischer, Claire Flaujac, Valérie Eschwège, Céline Delassasseigne, Léna Leflem, Frédéric Loridon, Sophie Voisin, Dominique Lasne","doi":"10.1080/09537104.2024.2427745","DOIUrl":"10.1080/09537104.2024.2427745","url":null,"abstract":"Light transmission aggregometry (LTA) is a method used to investigate platelet functions in platelet-rich plasma (PRP), notably when screening for platelet disorders. Various national guidelines and recommendations help in setting up the LTA test in specialized laboratories. However, due to the nature of the sample matrix and its subsequent specificities, more accurate positions are needed to achieve LTA accreditation according to the standard NF EN ISO 15 189. We reviewed guidelines and recommendations as they can be useful in the accreditation process, and we conducted a survey on LTA practice among members of the Société Française de Thrombose et d'Hémostase (SFTH) in 2021. We formulated 28 proposals, which have been approved by vote within the SFTH. All aspects to take into consideration for the proper conduct of LTA assays and their accreditation have been covered. Notably, preanalytical, analytical and postanalytical aspects are depicted, including blood sampling, PRP preparation, instruments, agonists, performance assessment, personnel training and data interpretation. This document, essentially representing a French position paper on the current recommendations and subsequent proposals for LTA accreditation, might prove useful also outside France for relevant laboratories and auditors involved in LTA accreditation.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2427745"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Appraising non-linear association between pre-diagnostic platelet counts and cancer survival outcomes: observational and genetic analysis. 评估诊断前血小板计数与癌症生存结果之间的非线性关联：观察和遗传分析。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-07-29 DOI: 10.1080/09537104.2024.2379815

Changtao Li, Junhua Chen, Deqian Han, Chi Shu, Jun Huang, Linru Wei, Haoran Luo, Qingbin Wu, Xin Chen, Yazhou He, Yanhong Zhou

{"title":"Appraising non-linear association between pre-diagnostic platelet counts and cancer survival outcomes: observational and genetic analysis.","authors":"Changtao Li, Junhua Chen, Deqian Han, Chi Shu, Jun Huang, Linru Wei, Haoran Luo, Qingbin Wu, Xin Chen, Yazhou He, Yanhong Zhou","doi":"10.1080/09537104.2024.2379815","DOIUrl":"https://doi.org/10.1080/09537104.2024.2379815","url":null,"abstract":"Previous studies have reported inconsistent associations between platelet count (PLT) and cancer survival. However, whether there is linear causal effect merits in-depth investigations. We conducted a cohort study using the UK Biobank and a two-sample Mendelian randomization (MR) analysis. PLT levels were measured prior to cancer diagnosis. We adopted overall survival (OS) as the primary outcome. Cox models were utilized to estimate the effects of PLTs on survival outcomes at multiple lag times for cancer diagnosis. We employed 34 genetic variants as PLT proxies for MR analysis. Linear and non-linear effects were modeled. Prognostic effects of gene expression harboring the instrumental variants were also investigated. A total of 65 471 cancer patients were included. We identified a significant association between elevated PLTs (per 100 × 109/L) and inferior OS (HR: 1.07; 95% CI: 1.04-1.10; p < .001). Similar significant associations were observed for several cancer types. We further observed a U-shaped relationship between PLTs and cancer survival (p < .001). Our MR analysis found null evidence to support a causal association between PLTs and overall cancer survival (HR: 1.000; 95% CI: 0.998-1.001; p = .678), although non-linear MR analysis unveiled a potential greater detrimental effect at lower PLT range. Expression of eleven PLT-related genes were associated with cancer survival. Early detection of escalated PLTs indicated possible occult cancer development and inferior subsequent survival outcomes. The observed associations could potentially be non-linear. However, PLT is less likely to be a promising therapeutic target.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2379815"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the morphine-platelet activity association in patients with ST-elevation myocardial infarction undergoing primary percutaneous coronary intervention. st段抬高型心肌梗死患者经皮冠状动脉介入治疗中吗啡-血小板活性的相关性研究。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-12-06 DOI: 10.1080/09537104.2024.2434225

Menikae K Heenkenda, Erik Träff, Tomas L Lindahl, Dimitrios Venetsanos, Joakim Alfredsson

{"title":"Exploring the morphine-platelet activity association in patients with ST-elevation myocardial infarction undergoing primary percutaneous coronary intervention.","authors":"Menikae K Heenkenda, Erik Träff, Tomas L Lindahl, Dimitrios Venetsanos, Joakim Alfredsson","doi":"10.1080/09537104.2024.2434225","DOIUrl":"https://doi.org/10.1080/09537104.2024.2434225","url":null,"abstract":"ST-segment elevation myocardial infarction (STEMI) is usually caused by a ruptured atherosclerotic plaque, with subsequent thrombus formation. Platelet inhibition and primary percutaneous coronary intervention (PCI) are essential treatments. Morphine, used to relieve pain and anxiety in STEMI patients, delays the onset of P2Y12 inhibitors. This study aimed to further explore the association between platelet activity and morphine treatment in patients with STEMI. In this sub-study of the VALIDATE-SWEDHEART trial, 89 STEMI patients treated with ticagrelor, and primary PCI were included. Platelet aggregation and biomarkers of platelet activity, coagulation, and inflammation (sP-selectin, thrombin-antithrombin complexes, prothrombin fragments 1 + 2, CD40L, CRP, beta-thromboglobulin, and pentraxin3) were assessed at three time points: before, one, and twelve hours after PCI. Of the 89 patients, 40 received morphine before hospital arrival. There were no significant differences in age, sex, medical history, or coronary disease extent. One hour after PCI, ADP-induced (36 vs 61, p < .001), arachidonic acid-induced (20 vs 36, p = .003), collagen-induced (48 vs 60, p = .03) aggregation, and the proportion of high on-treatment ADP-induced platelet reactivity (27% vs 60%, p = .001) were significantly higher in morphine-treated patients. No significant differences were found before or 12 hours after PCI. No significant differences in platelet activity biomarkers were observed. Morphine increased platelet aggregation in STEMI patients but did not affect biomarkers.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2434225"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case of acquired transient bleeding diathesis associated with acquired platelet storage pool deficiency and defective thromboxane A2 production. 一例与获得性血小板储存池缺乏症和血栓素 A2 生成缺陷有关的获得性一过性出血综合症。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-06-04 DOI: 10.1080/09537104.2024.2358241

Mariangela Scavone, Bianca Clerici, Eti Alessandra Femia, Claudia Ghali, Antonella Fioretti, Elena Bossi, Marco Cattaneo, Gian Marco Podda

{"title":"A case of acquired transient bleeding diathesis associated with acquired platelet storage pool deficiency and defective thromboxane A2 production.","authors":"Mariangela Scavone, Bianca Clerici, Eti Alessandra Femia, Claudia Ghali, Antonella Fioretti, Elena Bossi, Marco Cattaneo, Gian Marco Podda","doi":"10.1080/09537104.2024.2358241","DOIUrl":"10.1080/09537104.2024.2358241","url":null,"abstract":"Acquired disorders of platelet function are an underdiagnosed cause of bleeding tendency. A 14-year-old girl developed moderate mucocutaneous bleeding two weeks after a Mycoplasma pneumoniae infection successfully treated with clarithromycin. The patient was referred to us 7 months later for laboratory investigation of the persisting bleeding diathesis. The patient's personal and family histories were negative for bleeding disorders. Complete blood count, von Willebrand Factor levels and coagulation tests were normal; platelet aggregation, ATP secretion, δ-granules content and serum thromboxane B2 levels were defective. At follow-up visits, laboratory parameters and the bleeding diathesis progressively normalized within 2 years. The patient's condition is compatible with a diagnosis of acquired Storage Pool Deficiency (SPD), associated with defective thromboxane A2 production. To our knowledge, this is the first case of acquired, transient SPD with spontaneous remission. The pathogenic role of Mycoplasma pneumoniae infection or clarithromycin is possible, albeit uncertain.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2358241"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141238103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility and effectiveness of the prolonged use of eltrombopag in addition to immunosuppression in patients with acquired aplastic anemia: a single-center real-life experience. 在获得性再生障碍性贫血患者中，除免疫抑制外，长期使用伊曲巴格的可行性和有效性：单中心现实经验

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-10-16 DOI: 10.1080/09537104.2024.2415483

Monica Carpenedo, Arianna Zappaterra, Lorenzo Del Castello, Beatrice Ferrari, Giulia Cotilli, Davide Paolo Bernasconi, Sara Pezzatti, Filippo Sacco, Lorenza Borin, Andrea Carrer, Luisa Verga, Filippo Brioschi

{"title":"Feasibility and effectiveness of the prolonged use of eltrombopag in addition to immunosuppression in patients with acquired aplastic anemia: a single-center real-life experience.","authors":"Monica Carpenedo, Arianna Zappaterra, Lorenzo Del Castello, Beatrice Ferrari, Giulia Cotilli, Davide Paolo Bernasconi, Sara Pezzatti, Filippo Sacco, Lorenza Borin, Andrea Carrer, Luisa Verga, Filippo Brioschi","doi":"10.1080/09537104.2024.2415483","DOIUrl":"https://doi.org/10.1080/09537104.2024.2415483","url":null,"abstract":"Acquired Aplastic Anemia (AAA) is a rare disease involving primary bone marrow failure with consequent pancytopenia. The addition of the synthetic thrombopoietin-receptor agonist eltrombopag (ELT) to standard immunosuppression for the treatment of AAA has led to improvements in hemopoietic outcomes of AAA. Most of the data on the use of ELT for AAA was based on a maximum of 6 months of therapy. However, in clinical practice, a longer use of ELT is often required. This paper presents a monocentric real-life experience with prolonged use of ELT in 10 patients with AAA, showing data on effectiveness and safety. In our cohort, a high rate of response to ELT added to standard immunosuppression in patients with varying grades of severity of AAA was reported. After a median (range) observation time of 47.5 (31-75) months, the treatment with ELT was feasible with an overall response probability of 70% and was not associated with any concerning adverse event. Two episodes of relapse were reported; no signs of evolution have been reported so far. In conclusion, ELT as a dose-response-adjusted prolonged therapy associated with standard immunosuppression in AAA patients not eligible for transplant seems to be feasible to consolidate and maintain the response.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2415483"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet life cycle during aging: function, production and clearance. 衰老过程中血小板的生命周期：功能、产生和清除。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 DOI: 10.1080/09537104.2024.2433750

Friedrich Reusswig, Olga An, Carsten Deppermann

引用次数: 0

The efficacy of platelet rich plasma on women with poor ovarian response: a systematic review and meta-analysis. 富血小板血浆对卵巢反应不良妇女的疗效：系统综述和荟萃分析。

IF 3.3 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-01-12 DOI: 10.1080/09537104.2023.2292612

Lingling Wu, Fenfang Su, Peixin Luo, Qingqing Dong, Mengni Ma, Guangyong Ye

{"title":"The efficacy of platelet rich plasma on women with poor ovarian response: a systematic review and meta-analysis.","authors":"Lingling Wu, Fenfang Su, Peixin Luo, Qingqing Dong, Mengni Ma, Guangyong Ye","doi":"10.1080/09537104.2023.2292612","DOIUrl":"10.1080/09537104.2023.2292612","url":null,"abstract":"Background: Platelet-rich plasma (PRP) is a therapeutic approach that is gaining attention for its potential in the treatment of poor ovarian response. This meta-analysis aimed to systematically review and analyze clinical studies to evaluate the impact of PRP on poor responders undergoing ovarian stimulation for IVF.Methods: A comprehensive search was conducted in electronic databases, including PubMed, Embase, Scopus, Web of Science, and the Cochrane Library to identify relevant studies published in English. The pooled data, such as pregnancy outcome, number of MII oocytes, number of transferable embryos, and ovarian reserve markers were analyzed using R version 4.2.3.Results: A total of 10 trials were enrolled in the present meta-analysis. Following PRP treatment, live birth rate was found to be 16.6% (95% CI 8.8%-26.1%), while clinical pregnancy rate was observed to be 25.4% (95% CI 13.1%-39.9%). PRP pretreatment resulted in a higher number of MII oocytes (MD 1.073, 95% CI 0.720 to 1.427), a higher number of embryos (MD 0.946, 95% CI 0.569 to 1.323), a higher antral follicle count (MD 1.117; 95% CI 0.689 to 1.544), and the change of hormone levels.Conclusions: Among the studies evaluated in this review, PRP showed promising results in poor responder. Further research is required to clarify the potential role of PRP in female reproductive health.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2292612"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139425387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial and temporal characterization of cytoskeletal reorganizations in adherent platelets. 粘附血小板细胞骨架重组的空间和时间特征。

IF 2.5 3区医学

Platelets Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1080/09537104.2024.2422437

Clotilde Joubert, Alexei Grichine, Monika Dolega, Sophie Michallet, Florence Appaix, Isabelle Tardieux, Laurence Lafanechère, Karin Sadoul

{"title":"Spatial and temporal characterization of cytoskeletal reorganizations in adherent platelets.","authors":"Clotilde Joubert, Alexei Grichine, Monika Dolega, Sophie Michallet, Florence Appaix, Isabelle Tardieux, Laurence Lafanechère, Karin Sadoul","doi":"10.1080/09537104.2024.2422437","DOIUrl":"https://doi.org/10.1080/09537104.2024.2422437","url":null,"abstract":"The functional role of platelets is intricately linked to the dynamic organization of two main components of the cytoskeleton, microtubules and actin fibers. Throughout the phases of platelet activation, spreading, and retraction, both of these essential polymers undergo continuous and orchestrated reorganization. Our investigation of the dynamic cytoskeletal changes during these phases highlights a sequential remodeling of the actin cytoskeleton in adherent platelets from the formation of initial actin nodules through the development of stress fibers and a subsequent return to nodular structures. Concurrently, the marginal ring of microtubules, characteristic of resting platelets, undergoes a re-organization induced by marginal band extension and coiling toward the formation of star-like bundles of microtubules. Subsequently, these bundles are dispersed into individual microtubules, which are re-bundled at later stages before ring-like structures are formed again. These findings suggest a compelling tendency for both cytoskeletal components to revert to their original configurations. Notably, the early steps of platelet cytoskeleton reorganizations have previously been shown to be regulated by the signaling cascade triggered during platelet activation, which leads to an increase of cytosolic calcium concentrations. We show here that later steps are potentially regulated by a progressive decrease of intracellular calcium concentrations as platelets approach the end of their functional lifespan.","PeriodicalId":20268,"journal":{"name":"Platelets","volume":"35 1","pages":"2422437"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0