Carine E Hamo, Matthew Muller, Emily Rosenfeld, Yuhe Xia, Adedoyin Akinlonu, Elliot Luttrell-Williams, Tessa J Barrett, Jeffrey S Berger
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引用次数: 0
Abstract
Cardiometabolic risk factors, obesity, diabetes and hyperlipidemia contribute to cardiovascular disease (CVD). While platelets are involved in CVD pathogenesis, the relationship between risk factor burden on platelet indices and the platelet transcriptome remains uncertain. Blood was collected from CVD-free adults, measuring platelet count, mean platelet volume (MPV), immature platelet fraction (IPF), and absolute immature platelet fraction (AIPF) by hemogram. Platelets were isolated and analyzed via RNA sequencing. Participants were stratified by number of cardiometabolic risk factors (diabetes, obesity, hyperlipidemia). We calculated median (IQR) values of platelet indices and p-for-trend via linear regression across risk factor burden. To evaluate the association between risk factor burden and platelet transcripts, we performed multivariable linear regression adjusting for age, sex, and race/ethnicity. Among 141 participants, (50.5 ± 14.8 years, 42% male, 26% Black) risk factor burden was associated with increasing platelet size, IPF, and AIPF but not platelet count. Platelet RNA sequencing identified 100 differentially expressed transcripts (p < .01; 66 upregulated, 34 downregulated). Gene ontology enrichment analysis demonstrated upregulated pathways of secondary metabolic processes (NES = 1.96, p < .01), and hematopoietic stem cell proliferation (NES = 1.95, p < .01). Greater cardiometabolic risk factor burden is associated with increased platelet size and immaturity and suggesting novel platelet-mediated mechanisms linking risk factor burden with CVD.
心脏代谢危险因素,肥胖,糖尿病和高脂血症导致心血管疾病(CVD)。虽然血小板参与心血管疾病的发病机制,但血小板指标的危险因素负担与血小板转录组之间的关系仍不确定。采集无cvd成人血液,通过血象测定血小板计数、平均血小板体积(MPV)、未成熟血小板分数(IPF)和绝对未成熟血小板分数(AIPF)。分离血小板并通过RNA测序进行分析。根据心脏代谢危险因素(糖尿病、肥胖、高脂血症)的数量对参与者进行分层。我们通过跨危险因素负担的线性回归计算血小板指数的中位数(IQR)值和趋势p值。为了评估危险因素负担与血小板转录物之间的关系,我们进行了多变量线性回归,调整了年龄、性别和种族/民族。在141名参与者中,(50.5±14.8岁,42%男性,26%黑人)危险因素负担与血小板大小、IPF和AIPF增加有关,但与血小板计数无关。血小板RNA测序鉴定出100个差异表达转录物(p p p
期刊介绍:
Platelets is an international, peer-reviewed journal covering all aspects of platelet- and megakaryocyte-related research.
Platelets provides the opportunity for contributors and readers across scientific disciplines to engage with new information about blood platelets. The journal’s Methods section aims to improve standardization between laboratories and to help researchers replicate difficult methods.
Research areas include:
Platelet function
Biochemistry
Signal transduction
Pharmacology and therapeutics
Interaction with other cells in the blood vessel wall
The contribution of platelets and platelet-derived products to health and disease
The journal publishes original articles, fast-track articles, review articles, systematic reviews, methods papers, short communications, case reports, opinion articles, commentaries, gene of the issue, and letters to the editor.
Platelets operates a single-blind peer review policy. Authors can choose to publish gold open access in this journal.