{"title":"Isolates of Corynespora cassiicola, Causal Agent of Target Spot of Soybean, in Kentucky Contain the G143A Mutation Conferring Resistance to Quinone Outside Inhibitor Fungicides","authors":"D. Neves, Carl A. Bradley","doi":"10.1094/php-12-23-0108-br","DOIUrl":"https://doi.org/10.1094/php-12-23-0108-br","url":null,"abstract":"Target spot, caused by Corynespora cassiicola, is a soybean disease of increasing importance in the southern United States. Recently, isolates of C. cassiicola with resistance to quinone outside inhibitor (QoI) fungicides have been confirmed with the G143A mutation in multiple southern states, including Alabama, Arkansas, Mississippi, and Tennessee. From 2017 to 2019, a total of 84 isolates of C. cassiicola were recovered from soybean field in 12 counties in Kentucky. DNA sequencing of the cytochrome b gene of these isolates revealed that 15.5% of the isolates had the G143A mutation that confers resistance to QoI fungicides and were in 50% of the counties in which isolates originated. This represents the first report of QoI fungicide resistance in C. cassiicola isolates from Kentucky soybean fields. Considering these findings, Kentucky soybean growers should adopt target spot management practices which include rotating to non-host crops, planting resistant soybean cultivars, and applying fungicides from different fungicide classes.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140968231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Stenocarpella maydis and Stenocarpella macrospora inoculation timing on Diplodia ear rot of corn","authors":"Nolan Anderson, Kiersten A. Wise","doi":"10.1094/php-01-24-0008-rs","DOIUrl":"https://doi.org/10.1094/php-01-24-0008-rs","url":null,"abstract":"Stenocarpella maydis (Berk.) Sutton and Stenocarpella macrospora (Earle) Sutton are both causal agents of Diplodia ear rot (DER) of corn in the United States, however DER caused by S. macrospora has not been as well studied as S. maydis in trials examining disease development. Field experiments were established from 2019 to 2021 in Kentucky to examine the effect of inoculation timing at one of four growth stages (eight leaf collar (V8), silking (R1), blister (R2), milk (R3)) of each pathogen on DER severity, yield and test weight. Experiments were conducted under dryland and irrigated conditions in 2019 and 2020, and under irrigated conditions in 2021. Treatments inoculated by either pathogen at silking (R1) resulted in higher DER than treatments where inoculation occurred at milk stage (R3) in all years. Yield was reduced in treatments inoculated at R1 compared to the non-inoculated treatment in all trials and years, except the irrigated trial in 2019. A negative relationship was found between DER severity and corn yield and test weight: As DER severity increased, subsequent yield and test weight of corn decreased. This research indicates that an R1 inoculation timing of both Stenocarpella pathogens can negatively impact yield and grain quality.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140972397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rachel E. Rudolph, E. Dixon, Misbakhul Munir, Kimberly Leonberger, Kathryn Pettigrew, Martin Polo, Henry S. Smith, N. W. Gauthier
{"title":"Susceptibility of Zucchini Cultivars to Powdery Mildew (Podosphaera xanthii) under High and Low Nitrogen Regimes","authors":"Rachel E. Rudolph, E. Dixon, Misbakhul Munir, Kimberly Leonberger, Kathryn Pettigrew, Martin Polo, Henry S. Smith, N. W. Gauthier","doi":"10.1094/php-12-23-0105-rs","DOIUrl":"https://doi.org/10.1094/php-12-23-0105-rs","url":null,"abstract":"Powdery mildew, primarily caused by Podosphaera xanthii, threatens cucurbit crop yield and longevity. Fungicides and resistant cultivars cannot fully manage or prevent the disease. Previous work has shown that powdery mildew incidence and severity can increase under high soil nitrogen. This study was conducted in Kentucky to determine disease severity and yield using a split plot design with nitrogen fertility (high: 140 kg/ha, low: 84 kg/ha) and zucchini cultivar (Cash Machine, Cue Ball, Desert, Dunja, Eight Ball, Emerald Delight, Golden Glory, Mexicana, and Spineless Perfection) as the main plot and subplot factors, respectively. In year one, powdery mildew severity was significantly higher in high soil nitrogen and varied significantly among cultivars. Yield was significantly lower in high soil nitrogen. Significant differences in fruit count, but not fruit weight, were observed among the cultivars. In year two, nitrogen did not significantly affect disease severity, but fruit weight and count were significantly lower in the high soil nitrogen treatment. ‘Golden Glory’ had significantly higher powdery mildew severity while ‘Mexicana’ and ‘Eight Ball’ had significantly lower severity compared to all other cultivars. Spineless Perfection had the highest fruit weight and was significantly higher than all other cultivars except for Cue Ball and Eight Ball. ‘Golden Glory’ had the highest fruit count and was significantly higher than all others except for ‘Dunja’. Nitrogen did not consistently affect disease severity throughout the study. The effects of powdery mildew on yield should be explored further, as heavily diseased plants did not always have low yield.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141004107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cristian Olaya, Lauri Reinhold, McKenna Platt, A. Peetz, Kelly Donahue, I. Zasada
{"title":"Assessment of the Distribution of Dagger Nematodes and Associated Nematode Transmitted Viruses in Pacific Northwest Small Fruit Crops","authors":"Cristian Olaya, Lauri Reinhold, McKenna Platt, A. Peetz, Kelly Donahue, I. Zasada","doi":"10.1094/php-04-24-0034-rs","DOIUrl":"https://doi.org/10.1094/php-04-24-0034-rs","url":null,"abstract":"Small fruit production is an important component of the agricultural sector in the Pacific Northwest of the United States (Oregon and Washington). A diversity of small fruits is produced in the region including wine grape, blueberry, strawberry, and caneberry (red raspberry, black raspberry, and blackberry). Plant-parasitic nematodes and viruses both impact the production of small fruits in the region; there is not an up-to-date understanding of the distribution of potential virus-vectoring dagger nematodes (Xiphinema spp.) and associated nematode-transmitted viruses. Soil and leaf samples were collected from 43 wine grape vineyards and 24 blueberry, 23 caneberry, 5 strawberry and one currant field (s) in 2021 and 2023. The samples were analyzed to determine the presence of dagger nematodes and the nepoviruses tomato ringspot virus (ToRSV) and tobacco ringspot virus (TRSV). Dagger nematodes were found in 52% of the fields. ToRSV was detected in two blueberry fields in Washington, while in Oregon ToRSV was detected in one field each of blueberry, red raspberry, currant, and wine grape. TRSV was not conclusively found in any of the surveyed samples. To the best of our knowledge, this is the first time ToRSV is reported in wine grapes in Oregon and in the red currant species, Ribes spicatum. This study provides an up-to-date assessment of the distribution of dagger nematodes and nepoviruses associated with small fruits in the Pacific Northwest.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141018287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Influence of Sprinkler and Drip Irrigation on the Incidence and Severity of Bacterial Disease in Onions Grown in Northeast California","authors":"Rob G. Wilson, Brenna J. Aegerter, Gabe LaHue","doi":"10.1094/php-01-24-0002-rs","DOIUrl":"https://doi.org/10.1094/php-01-24-0002-rs","url":null,"abstract":"Overhead sprinkler irrigation and its effect on leaf wetness is often referenced as a key factor for bacterial disease spread in onions grown in arid climates. A study was conducted in 2021 and 2022 in Tulelake, CA to compare the incidence and severity of bacterial disease under sprinkler and drip irrigation. The experiment was a split-plot randomized complete block design with drip and solid-set sprinkler as the main plot treatments and inoculated and non-inoculated with plant pathogenic bacteria as the split-plot treatments. The entire study area was irrigated with solid-set sprinklers from planting until the five-leaf stage to facilitate uniform onion germination and chemigation of herbicides for weed control. After the five-leaf stage, irrigation treatments were imposed for the remainder of the growing season. Total irrigation water amount and irrigation frequency were similar for both irrigation methods. Foliar disease severity and the incidence of bulb rot were 170% and 186% higher, respectively, for solid-set sprinkler compared to drip. Leaf wetness within the plant canopy during onion bulbing was on average 36% higher in solid-set sprinkler compared to drip while relative humidity and temperature were similar between irrigation methods. Drip maintained lower soil water tension and increased average bulb size by 9% compared to solid-set sprinkler. This study confirms historic observations that overhead irrigation can extend leaf wetness and increase bacterial disease in onions in arid production areas, and highlights the potential benefits associated with the adoption of drip irrigation.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140690441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jada S. Powlen, M. Fidanza, J. Kerns, Lori A. Hoagland, C. Bigelow
{"title":"Turf-Type Tall Fescue Brown Patch Resistance as Influenced by Morphological Characteristics","authors":"Jada S. Powlen, M. Fidanza, J. Kerns, Lori A. Hoagland, C. Bigelow","doi":"10.1094/php-10-23-0082-rs","DOIUrl":"https://doi.org/10.1094/php-10-23-0082-rs","url":null,"abstract":"Brown patch (caused by multiple species of Rhizoctonia and Rhizoctonia-like fungi) is one of the major turfgrass diseases impacting turf-type tall fescue [ Schedonorus arundinaceus (Schreb.) Dumort., nom. cons.] lawns during the summer season. Selection of tall fescue cultivars with brown patch resistance is an important cultural management practice to reduce disease severity and limit reliance on fungicides. A greenhouse experiment was conducted to quantify differences in brown patch resistance among 15 tall fescue cultivars and correlate disease resistance with plant morphological characteristics. Prior to R. solani inoculation, leaf blade width, sheath width and length, endophyte stem infection, and relative growth rate were measured. Disease resistance was determined as a percentage of the canopy blighted as measured using digital image techniques. Brown patch severity after 21 days in Experiments 1 and 2 ranged from 36.7 to 72.7% and 20.8 to 41.8%, respectively, among all cultivars. There were significant differences among morphological characteristics for the cultivars. Increased brown patch was associated with cultivars with a faster growth rate, wider sheath width, and decreased sheath length. This study demonstrates the importance of cultivar selection for reducing brown patch severity in tall fescue lawns. Additionally, tall fescue cultivars with higher shoot density and narrow leaves should not be avoided due to concerns of increased brown patch susceptibility.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140694644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diagnostic Guide for Sclerotial Blight and Circular Spot of Sweetpotato","authors":"Jack Mascarenhas, Lina Quesada-Ocampo","doi":"10.1094/php-12-23-0110-dg","DOIUrl":"https://doi.org/10.1094/php-12-23-0110-dg","url":null,"abstract":"Agroathelia rolfsii (anamorph: Sclerotium rolfsii) is a soilborne fungal pathogen that can cause disease on over 500 documented host species, including economically important field and vegetable crops. While the pathogen commonly infects the stem or crown of most hosts, it is also capable of damaging fruit and root structures that are near the soil line, resulting in wilting, stunting, and plant death. Two diseases caused by this pathogen are sclerotial blight and circular spot, both of which are detrimental for sweetpotato production. A. rolfsii is a necrotrophic pathogen and can be cultured from susceptible hosts and on artificial media. The purpose of this diagnostic guide is to provide characteristic traits for identifying A. rolfsii in sweetpotato as well as outline methods for pathogen isolation, morphological and molecular characterization, culture maintenance and long-term storage, and pathogenicity testing.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140696905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of a novel fungicidal extract from blue spruce, Picea pungens","authors":"Yonghong Guo, Bin Zhou, Margaret Pooler","doi":"10.1094/php-10-23-0089-rs","DOIUrl":"https://doi.org/10.1094/php-10-23-0089-rs","url":null,"abstract":"Aqueous extracts from 22 species of evergreen plants and 32 accessions of spruce (Picea A. Dietr.) were screened using an in vitro assay to determine their anti-fungal activity against Calonectria henricotiae, one of the causal agents of boxwood blight. Most of the evergreen species and all the spruce accessions showed significant antifungal activity (P<0.05); in particular, the extract from one accession of Picea pungens Engelm. had significant effective fungistatic and fungicidal activity (P<0.05), both in vitro and when tested on detached boxwood leaves and whole boxwood plants. The extraction process was simple, consisting of autoclaving fresh leaves in water, and once extracted, the active compound was stable in aqueous solution and when lyophilized and reconstituted. Further investigations will focus on the potential of this extract as an alternative to traditional fungicides for use in a wide range of crops and in diverse applications from controlling fungal infections in the landscape to preventing post-harvest losses in fruit and vegetable crops.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140712186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdelrazek S. Abdelrhim, Douglas S. Higgins, M. Hausbeck
{"title":"Occurrence of Stemphylium leaf spot caused by Stemphylium vesicarium in commercial celery production following an onion rotation","authors":"Abdelrazek S. Abdelrhim, Douglas S. Higgins, M. Hausbeck","doi":"10.1094/php-07-23-0067-rs","DOIUrl":"https://doi.org/10.1094/php-07-23-0067-rs","url":null,"abstract":"In 2022, lesions were observed on the lower leaves of ‘Fandango’ celery (Apium graveolens L) growing in a commercial field located in Hamilton, MI. Brown spots were observed on the leaves’ adaxial surface and border extending to the petiole. Conidia were observed on the symptomatic tissue and 25 fungal isolates, morphologically similar to S. vesicarium, were obtained. DNA was extracted from three isolates and two primer sets were used to amplify and sequence the internal transcribed spacer (ITS) region and partial calmodulin (cmdA) and glyceraldehyde-3-phosphate dehydrogenase (gapdh) genes. The obtained sequences of the three isolates had 100% pairwise identity with S. vesicarium sequences MW798751 (ITS), MK675706 (cmdA) and OQ925923 (gapdh). A multilocus phylogenetic analysis (neighbor-joining tree) strongly supported clustering Michigan isolates into a single clade with S. vesicarium reference sequences. Isolates recovered from celery were pathogenic to ‘CR1’, ‘Tall Utah’ and ‘Challenger’ celery and ‘Bradley’ onion. S. vesicarium isolates isolated from symptomatic onion volunteers were pathogenic to the three celery cultivars. To our knowledge, this is the first report of Stemphylium vesicarium as a pathogen on celery causing leaf spot, and volunteer onion may be a source of Stemphylium in celery fields.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140753203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Henry S. Smith, E. Dixon, Misbakhul Munir, N. W. Gauthier
{"title":"Incidence and Distribution of the Fusarium Sambucinum Species Complex associated with Cannabis sativa in Kentucky","authors":"Henry S. Smith, E. Dixon, Misbakhul Munir, N. W. Gauthier","doi":"10.1094/php-12-23-0106-s","DOIUrl":"https://doi.org/10.1094/php-12-23-0106-s","url":null,"abstract":"Hemp is an emerging crop that is cultivated for a variety of applications. Since commercial reintroduction in 2018 many pathogens and pests have been identified that threaten production, such as Fusarium head blight (FHB). The disease causes extensive blighting of floral tissues and presents a mycotoxin contamination risk. During the 2022 growing season 25 sites were surveyed across 15 different counties to determine the incidence and distribution of toxigenic Fusarium species within the Fusarium Sambucinum species complex (FSAMSC). Fusarium graminearum was the most isolated species within the complex. The 15-DON chemotype was found to be the predominant F. graminearum population. The FSAMSC isolation rate varied by site and reached up to 46.6%. Isolation rates were found to be correlated with plant age and calendar day. Management strategies are currently limited for FHB of hemp. Data from this survey will help develop future projects and disease management plans regarding spray timings and crop rotation.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140368155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}