First report of Hirsutella thompsonii as an entomopathogenic fungus of hemp russet mite (Aculops cannabicola Farkas) in Kentucky

IF 1.7 Q2 PLANT SCIENCES

Plant Health Progress Pub Date : 2023-08-10 DOI:10.1094/php-04-23-0035-br

María Magdalena Ricciardi, Henry S. Smith, Z. Viloria, E. Dixon, D. Szarka, Bob Pearce, R. Villanueva, N. Gauthier

引用次数: 0

Abstract

The hemp russet mite (HRM) is a damaging pest of indoor/greenhouse hemp (Cannabis sativa L.) with limited tools for management. In 2021, HRM was identified in University of Kentucky hemp field trials. Large numbers of darkened, dead HRM were observed on leaves and peduncles. Samples of the HRM were disinfested and plated on potato dextrose agar (PDA). Fungal identity was confirmed by amplifying the internal transcribed spacer of the rDNA followed by Sanger sequencing. The isolated fungus was identified as Hirsutella thompsonii with 100% identity. Pathogenicity was validated using detached leaves infested with healthy HRM. Only HRM from the H. thompsonii-inoculated treatment developed fungal growth, while HRM from the controls did not. This study confirmed the pathogenicity of H. thompsonii on HRM. Further research is warranted to improve our understanding of the potential use and efficacy of H. thompsonii as a sustainable management tool.

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美国肯塔基州大麻赤藓（Aculops cannabicola Farkas）昆虫病原真菌汤普森Hirsutella thompsonii的首次报道

麻赤褐色螨(HRM)是室内/温室大麻(Cannabis sativa L.)的一种有害害虫，管理手段有限。2021年，在肯塔基大学的大麻田间试验中发现了人力资源管理。在叶片和花梗上观察到大量变暗的、死亡的HRM。对HRM样品进行消毒，并将其涂于马铃薯葡萄糖琼脂(PDA)上。通过扩增rDNA的内部转录间隔段，然后进行Sanger测序，确认真菌身份。分离得到的真菌鉴定为thompsonii毛杆菌，鉴定率100%。致病性用健康HRM侵染的离体叶片进行验证。只有接种thompsoni处理的HRM出现真菌生长，而对照组的HRM没有。本研究证实了thompsonii对HRM的致病性。进一步的研究是必要的，以提高我们的理解潜在的用途和功效的汤普森嗜血杆菌作为一个可持续的管理工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Health Progress Agricultural and Biological Sciences-Horticulture

CiteScore

3.00

自引率

13.00%

发文量

期刊介绍： Plant Health Progress, a member journal of the Plant Management Network, is a multidisciplinary science-based journal covering all aspects of applied plant health management in agriculture and horticulture. Both peer-reviewed and fully citable, the journal is a credible online-only publication. Plant Health Progress is a not-for-profit collaborative endeavor of the plant health community at large, serving practitioners worldwide. Its primary goal is to provide a comprehensive one-stop Internet resource for plant health information.