Phytomedicine最新文献

{"title":"Natural products in rheumatoid arthritis: Cell type-specific mechanisms and therapeutic implications","authors":"Yang Liao ,&nbsp;Xian Xiao ,&nbsp;Wen Cheng ,&nbsp;Yiyuan Wang ,&nbsp;Jiayan Lu ,&nbsp;Jingtao Zhang ,&nbsp;Jiaqi Zhang ,&nbsp;Peiyu Liu ,&nbsp;Liang Liu ,&nbsp;Hudan Pan","doi":"10.1016/j.phymed.2025.156980","DOIUrl":"10.1016/j.phymed.2025.156980","url":null,"abstract":"<div><h3>Background</h3><div>The pathogenesis of rheumatoid arthritis (RA) involves abnormal activation and interactions among various cell types. Traditional Chinese medicine (TCM) natural products show therapeutic potential with multi-target anti-inflammatory effects, but their specific impacts on RA-related cell types remain unclear.</div></div><div><h3>Purpose</h3><div>This review aims to provide a comprehensive overview of the effects of natural products on key cell types in RA.</div></div><div><h3>Methods</h3><div>We extensively searched PubMed, Web of Science, and Scopus until April 2025, using keywords like \"natural products,\" \"rheumatoid arthritis,\" \"cellular mechanisms,\" and specific cell types. Studies on natural products' effects on RA-related cells were included and analyzed.</div></div><div><h3>Results</h3><div>This review marks the first attempt to integrate cellular resolution in evaluating therapeutic mechanisms of natural products in RA. The key findings highlight the cell-type-specific effects and mechanistic underpinnings of natural products. Sinomenine preferentially inhibits fibroblast-like synoviocytes (FLS) proliferation via toll-like receptor 4 (TLR4) /MyD88/NF-κB blockade, whereas icariin reprograms macrophage polarization through ERK/HIF-1α/GLUT1 signaling pathway. Besides, natural products exhibit shared mechanisms across different cell types, such as oxidative stress and inflammatory pathway regulation. Importantly, certain natural products exhibit pan-cellular effects, targeting multiple cell types involved in RA pathogenesis. For example, resveratrol acts on a wide range of cell types including FLS, human umbilical vein endothelial cells (HUVECs), T cells, osteoclasts, chondrocytes, macrophages, monocytes, osteoblasts, and neutrophils.</div></div><div><h3>Conclusions</h3><div>Through an in-depth analysis of cellular mechanisms, this work highlights the specific regulatory effects of natural products on key cell types and their extensive influence across cellular networks. By examining these effects at the cellular level, this review establishes a new framework for developing novel RA therapeutics with increased cell selectivity, thus advancing precision phytomedicine and translational research.</div></div>","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"145 ","pages":"Article 156980"},"PeriodicalIF":6.7,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quinacrine induces autophagy via the Dlg5/AKT pathway to inhibit osteosarcoma cell proliferation and suppresses migration and invasion through the Dlg5/Girdin pathway","authors":"Hongyu Li ,&nbsp;Wenkai Chen ,&nbsp;Xiangchen Zeng ,&nbsp;Ronghai He,&nbsp;Guangrong Ji","doi":"10.1016/j.phymed.2025.156981","DOIUrl":"10.1016/j.phymed.2025.156981","url":null,"abstract":"<div><h3>Background</h3><div>Osteosarcoma (OS) is a malignant tumor of bone that originates from mesenchymal cells. Although improvements have been made in the survival rate of five years, the outlook for individuals with metastatic or recurrent disease remains unfavorable, highlighting the need for new therapeutic strategies and drug candidates. Quinacrine (QC) is a plant-derived bioactive compound extracted from the bark of the cinchona tree, has shown promising anticancer properties by suppressing the proliferation of cancer cells, inducing autophagy, and overcoming resistance to treatment. However, its potential therapeutic application in OS has yet to be thoroughly investigated.</div></div><div><h3>Purpose</h3><div>In this study, we investigated the inhibitory effects of QC, a plant-derived bioactive compound extracted from the bark of the cinchona tree, on the proliferation and migration of osteosarcoma cells and its potential mechanisms.</div></div><div><h3>Methods</h3><div>This study comprehensively evaluated the impact of QC on OS cells using both in vitro and in vivo models. Specific molecular pathways of OS cell growth inhibition by Quinacrine revealed by RNA-seq analysis. The inhibitory effect of QC on tumor growth and its potential organ toxicity were systematically investigated by constructing an in vivo mouse tumor model.</div></div><div><h3>Results</h3><div>The outcomes suggested that QC notably suppressed the growth, migration, and invasion of 143B and MG63 OS cells, causing the arrest of the cell cycle at the G2/M checkpoint. RNA sequencing analysis showed that QC inhibited AKT phosphorylation and induced autophagy in OS cells by increasing the expression of the Dlg5 gene. Furthermore, silencing the Dlg5 gene promoted OS cell proliferation and reduced the inhibitory effect of QC, thereby confirming the importance of Dlg5 in mediating its effects. This study also validated the molecular pathway by which QC inhibits OS cell migration and invasion by modulating the Dlg5/Girdin signaling pathway. In vivo, QC treatment led to a significant decrease in tumor growth in mice, with no evident organ toxicity observed.</div></div><div><h3>Conclusion</h3><div>In summary, QC suppresses the OS cells' growth by regulating the Dlg5/AKT signaling pathway and suppresses migration and invasion through the Dlg5/Girdin Pathway, emphasizing its potential as a therapeutic agent for OS.</div></div>","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"145 ","pages":"Article 156981"},"PeriodicalIF":6.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ranunculus ternatus Thunb. alkaloids attenuate colorectal cancer metastasis through EMT suppression","authors":"Yuchan Li ,&nbsp;Liang Ye ,&nbsp;Shixin Li ,&nbsp;Bing Yang ,&nbsp;Jian Li ,&nbsp;Xiaobin Jia ,&nbsp;Liang Feng","doi":"10.1016/j.phymed.2025.156965","DOIUrl":"10.1016/j.phymed.2025.156965","url":null,"abstract":"<div><h3>Background</h3><div>Distant metastasis remains a major contributor to treatment failure and poor prognosis in colorectal cancer (CRC), emphasizing the urgent need for novel anti-metastatic strategies. Ranunculus ternatus Thunb. (RT), a traditional herbal medicine historically used to treat tuberculosis, has recently shown emerging potential in oncology. However, its efficacy against CRC metastasis remains uninvestigated, and the pharmacologically active components along with the underlying mechanisms responsible for its anti-CRC and metastasis-inhibitory effects are yet to be elucidated.</div></div><div><h3>Purpose</h3><div>This study aims to investigate the anti-metastatic efficacy of bioactive components derived from RT and elucidate their mechanisms of action against CRC.</div></div><div><h3>Methods</h3><div>The active fraction of RT was identified using HCT116 and CT26 cell lines, alongside ectopic tumor-bearing mouse models. The chemical composition of this fraction was characterized by UPLC-Q-TOF/MS analysis. Transcriptomics profiling integrated with KEGG pathway enrichment analysis was performed, followed by core target prediction using CytoHubba and MCODE algorithms. RT-PCR, ELISA and Western Blot assays were employed for target validation. Drug-target interactions were confirmed via the cellular thermal shift assay (CETSA). The anti-metastatic effects of RT alkaloids (RTAs) were further evaluated through Transwell invasion assays, wound healing experiments, and tail vein injection-induced metastatic murine models.</div></div><div><h3>Results</h3><div>Both in vivo and in vitro experiments revealed that alkaloids from RT (RTAs) are the principal active ingredients exerting anti-CRC effects. Transcriptomic profiling and bioinformatics analyses demonstrate that RTAs inhibit epithelial-mesenchymal transition (EMT), with MMP3 and CCL5 identified as critical targets. Validation assays confirmed that RTAs significantly reduced levels of TGF-β and CCL5 in tumor tissues and serum of CRC-bearing mice. In addition, RTAs downregulated MMP3, MMP9, and β-catenin, while upregulating E-cadherin expression. These findings suggest that RTAs inhibit metastasis by modulating multiple EMT-related pathways. Furthermore, both in vitro and in vivo assays confirmed the ability of RTAs to significantly suppress distant metastasis in CRC models.</div></div><div><h3>Conclusion</h3><div>This study provides the first experimental evidence that RT exerts anti-metastatic effects against CRC. The bioactive alkaloid fraction (RTAs) mitigates CRC progression and metastasis by regulating the EMT process, highlighting its potential as a promising therapeutic candidate for metastatic CRC treatment.</div></div>","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"145 ","pages":"Article 156965"},"PeriodicalIF":6.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of pollenin B in asthma: PPAR-γ-mediated regulation of inflammatory pathways and arachidonic acid metabolism","authors":"Yuanyuan Wu ,&nbsp;Mengnan Zeng ,&nbsp;Xinmian Jiao ,&nbsp;Xinyi Ma ,&nbsp;Haoyu Wang ,&nbsp;Yanling Chen ,&nbsp;Xiaoke Zheng ,&nbsp;Weisheng Feng","doi":"10.1016/j.phymed.2025.156975","DOIUrl":"10.1016/j.phymed.2025.156975","url":null,"abstract":"<div><h3>Background</h3><div>Asthma poses a significant global health challenge, characterized by increasing prevalence and treatment complexities. Although <em>Ephedrae Herba</em> has long been used in asthma therapy, its pharmacological basis and mechanism of action remain unclear.</div></div><div><h3>Purpose</h3><div>This study aimed to clarify the protective effects of pollenin B (PolB), a compound extracted from <em>Ephedrae Herba</em>, in asthmatic mice and to thoroughly explore its possible action pathways and molecular mechanisms. This study supplements pharmacological research on the anti-asthmatic effects of <em>Ephedrae Herba</em> and provides more information for the development of novel and highly effective drugs for the treatment of asthma.</div></div><div><h3>Methods</h3><div>An ovalbumin (OVA)-induced asthma model was used to evaluate the effects of PolB on asthma symptoms and lung histopathology. Furthermore, cytokine profiles in the serum and bronchoalveolar lavage fluid (BALF), immune cell populations, and serum metabolomics data were analyzed to identify the pathways involved in PolB activity. PPAR-<em>γ</em> was identified as a key target, and its interaction with PolB was confirmed by surface plasmon resonance (SPR) analysis. A PPAR-<em>γ</em> inhibitor (T0070907) was administered in vivo, and combined PPAR-<em>γ</em> agonist (rosiglitazone)/inhibitor (T0070907) assays were conducted in vitro to validate the mechanisms involved.</div></div><div><h3>Results</h3><div>PolB improved asthma symptoms, modulated cytokine levels in the serum and BALF, modulated immune cells, and influenced arachidonic acid metabolism. Further mechanistic exploration indicated that PolB mitigated airway inflammation by regulating arachidonic acid metabolism and the JAK-STAT pathway via PPAR-<em>γ</em>.</div></div><div><h3>Conclusion</h3><div>PolB exerts anti-asthmatic effects via PPAR-<em>γ</em>-mediated regulation of key pathways. These findings not only enrich modern pharmacological research on the anti-asthmatic properties of <em>Ephedrae Herba</em> but also deepen our understanding of asthma pathogenesis, thus providing new insights into the optimization of asthma prevention and treatment.</div></div>","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"145 ","pages":"Article 156975"},"PeriodicalIF":6.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “ Alizarin attenuates oxidative stress-induced mitochondrial damage in vascular dementia rats by promoting TRPM2 ubiquitination and proteasomal degradation via Smurf2” [Phytomedicine Phytomedicine 135 (2024) 156119]","authors":"Guo-pin Pan ,&nbsp;Yan-hua Liu ,&nbsp;Ming-xu Qi ,&nbsp;Ya-qi Guo ,&nbsp;Zhen-lei Shao ,&nbsp;Hui-ting Liu ,&nbsp;Yi-wen Qian ,&nbsp;Shuang Guo ,&nbsp;Ya-ling Yin ,&nbsp;Peng Li","doi":"10.1016/j.phymed.2025.156947","DOIUrl":"10.1016/j.phymed.2025.156947","url":null,"abstract":"","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"144 ","pages":"Article 156947"},"PeriodicalIF":6.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jiegeng Gancao Decoction Ameliorates Ulcerative Colitis: An Integrative Approach Combining Network Pharmacology and Proteomics via in silico and in vivo studies","authors":"Ruipeng Shi ,&nbsp;Sio I Chan ,&nbsp;Haochun Jin ,&nbsp;Wei Li ,&nbsp;Lili Zhang ,&nbsp;Jinglin Yang ,&nbsp;Ruichen Wang ,&nbsp;Xiaoting Zheng ,&nbsp;Guozhen Cui ,&nbsp;Zhangfeng Zhong","doi":"10.1016/j.phymed.2025.156972","DOIUrl":"10.1016/j.phymed.2025.156972","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Ulcerative colitis (UC) is a chronic inflammatory condition of the colon that significantly affects the quality of life of patients. Although current treatments offer some relief, they are often accompanied by side effects and limited long-term efficacy. &lt;em&gt;Jiegeng Gancao&lt;/em&gt; Decoction (JGD), a Chinese classical prescription composed of Platycodon and Licorice, are potential alternative treatments with minimal side effects.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Purpose&lt;/h3&gt;&lt;div&gt;This present study aimed to establish a novel strategy for herbal medicine research, using JGD as a case study to improve the prediction efficiency and accuracy of network pharmacology and leveraging big data to accelerate herbal medicine research and development.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Study design&lt;/h3&gt;&lt;div&gt;Given the unmet clinical needs in the treatment of UC and the promising potential of JGD, this study integrated chemical profiling, network pharmacology predictions, and proteomic analysis to establish a model to explore the therapeutic mechanisms of JGD for the treatment of UC.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;UHPLC-Q-Exactive Orbitrap/MS was used to analyze the chemical components of JGD and its serum metabolites. Big data and network pharmacology were used to predict targets and pathways. A mouse model of DSS-induced colitis was established and were administered by JGD orally at 1.85 mg/kg daily. Therapeutic outcomes were evaluated through colon length detection, body weight monitoring, Disease Activity Index (DAI) scoring, and histopathological assessments. Enzyme-linked immunosorbent assay (ELISA), proteomics, flow cytometry, and western blotting were used to explore the underlying mechanism of the anti-colitis effects of JGD.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The chemical composition of JGD was analyzed to identify key bioactive compounds using UHPLC-Q-Exactive Orbitrap/MS. These compounds were evaluated using big data and network pharmacology, incorporating four data sources. Compounds were sourced from Herb: 102, Serum: 21, Review: 117, and Database: 99. This integration generated 72 combos and 864 datasets related to the Gene, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. JGD treatment significantly attenuated DSS-induced colitis in mice by improving body weight, colon length, inflammatory response, and intestinal barrier. Furthermore, proteomics and western blotting experiments confirmed that JGD attenuated DSS-induced down-regulated protein levels of SLC6A14 and RAGE. By screening the components of JGD via docking with RAGE and SLC6A14, Glycyrrhetic acid 3-O-glucuronide, Kaempferol-3-O-rutinoside, and Glyasperin A were identified as the main pharmacological substances that underlie the anti-colitis effects of JGD.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;Combining data from herb extracts and sources from the literature significantly improved prediction efficiency and accuracy. The results highlight the thera","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"145 ","pages":"Article 156972"},"PeriodicalIF":6.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moslae Herba extract alleviates hyperuricemia by regulating uric acid metabolism and relieving renal inflammation and fibrosis in mice","authors":"Jian Guo ,&nbsp;Huanhuan Liu ,&nbsp;Tong Jin ,&nbsp;Jinhui Jia ,&nbsp;Wenxiu Zhu ,&nbsp;Xiaodong Xia","doi":"10.1016/j.phymed.2025.156974","DOIUrl":"10.1016/j.phymed.2025.156974","url":null,"abstract":"<div><h3>Background</h3><div>Hyperuricemia (HUA) is a metabolic disease caused by uric acid metabolism disorder and is prevalent worldwide. Moslae Herba is a traditional herbal medicine known for its anti-inflammatory, antioxidant, and diuretic effects.</div></div><div><h3>Purpose</h3><div>This study aimed to investigate the effects and potential mechanisms of Moslae Herba extract (MHE) on alleviating HUA in mice.</div></div><div><h3>Methods</h3><div>The ingredients of MHE were analyzed using UHPLC-QE-MS/MS. The HUA mouse model was established by potassium oxonate (PO) and hypoxanthine (HX) to evaluate the anti-HUA effect of MHE. Molecular docking, <em>in vitro</em> enzyme inhibition assays and microscale thermophoresis (MST) were performed to assess the inhibitory effects of ingredients on xanthine oxidase (XOD). Targets and signaling pathways regulated by MHE were predicted through network pharmacology analysis. Fecal 16S rRNA gene sequencing was used to analyze alterations in gut microbiota.</div></div><div><h3>Results</h3><div>14 ingredients of MHE were identified using UHPLC-QE-MS/MS. MHE effectively alleviated HUA in mice induced by PO and HX. Mechanistically, MHE dually regulated XOD and ATP-binding cassette subfamily G member 2 (ABCG2) to decrease synthesis and promote intestinal and renal excretion of uric acid. Network pharmacology analysis and protein-level validation indicated that MHE relieved HUA-induced renal inflammation and fibrosis by suppressing the NLRP3 inflammasome and JAK2/STAT3 signaling pathway. 16S rRNA gene sequencing results suggested that MHE might further alleviate HUA and maintain intestinal homeostasis by modulating the gut microbiota.</div></div><div><h3>Conclusion</h3><div>This study is the first to demonstrate that MHE exerts anti-HUA effects through multiple mechanisms, providing novel insights for the phytotherapeutic management of HUA.</div></div>","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"145 ","pages":"Article 156974"},"PeriodicalIF":6.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginger-processed Magnoliae Officinalis Cortex ameliorates ovalbumin-induced asthma by alleviating inflammation via the gut-lung axis","authors":"Jiaqi Li,&nbsp;Jiaqi Hu,&nbsp;Dongliang Zhuo,&nbsp;Li Yang,&nbsp;Lan Wang,&nbsp;Bin Yang","doi":"10.1016/j.phymed.2025.156971","DOIUrl":"10.1016/j.phymed.2025.156971","url":null,"abstract":"<div><h3>Background</h3><div>The complex pathophysiology of asthma and the lack of effective therapies have driven research into natural product remedies. Ginger-processed Magnoliae Officinalis Cortex (GMOC), a traditional Chinese medicine, has been used for asthma treatment, but the lack of its potential mechanism of action limits the clinical application of GMOC.</div></div><div><h3>Purpose</h3><div>To investigate the mechanism of action of GMOC in asthmatic mice based on the gut-lung axis.</div></div><div><h3>Methods</h3><div>The anti-asthma effects of GMOC were evaluated in an ovalbumin (OVA)-induced mouse model of asthma. The lung and gut microbiota were analysed <em>via</em> 16S rRNA gene sequencing and short-chain fatty acids (SCFAs) were determined using GC/MS. Protein expression was evaluated by western blotting. Additionally, the chemical profile of GMOC was elucidated using LC/MS. Candidate compounds targeting target proteins were screened using <em>in silico</em> analysis, and an <em>in vitro</em> experiment was used to preliminarily verify the results.</div></div><div><h3>Results</h3><div>GMOC mitigated lung inflammation, mucus hypersecretion, and airway hyperresponsiveness (AHR) in asthmatic mice. It modulated the lung and gut microbiota and increased the levels of SCFAs in the colon, resulting in a reduction in inflammatory responses. In addition, GMOC downregulated transient receptor potential (TRP) channels and key proteins in the PI3K/AKT pathway in the lung and colon. The communication between TRPs and the PI3K/AKT pathway was further investigated <em>in vitro</em> using honokiol, the main compound in GMOC. Further, GMOC upregulated the expression of junction proteins in the lungs and colon to protect the epithelial barrier.</div></div><div><h3>Conclusion</h3><div>Crosstalk between organs (lung-gut-microbiota) and proteins (TRPs, junction proteins, and proteins in the PI3K/AKT pathway) contributes to the anti-inflammatory activities of GMOC in asthmatic mice.</div></div>","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"145 ","pages":"Article 156971"},"PeriodicalIF":6.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers and potential mechanisms of Chinese medicine compound (Chuanhong Zhongfeng Capsule) in the treatment of acute cerebral infarction","authors":"Yingyue Ding ,&nbsp;Jinwen Yao ,&nbsp;Xu Wang ,&nbsp;Liming Liu ,&nbsp;Ping Niu ,&nbsp;Hongge Ma ,&nbsp;Linlin Zhang ,&nbsp;Xiyuan Wang ,&nbsp;Huirong Yang ,&nbsp;Dexi Zhao ,&nbsp;Hongmei Nan ,&nbsp;Yue Wang","doi":"10.1016/j.phymed.2025.156955","DOIUrl":"10.1016/j.phymed.2025.156955","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;Acute Cerebral Infarction (ACI) is characterized by high disability and recurrence rates, posing a significant threat to public health. The Chuanhong Zhongfeng (CHZF) Capsule, developed by national medical master Jixue Ren, has been clinically proven to promote neurological function recovery, reduce disability rates, and improve long-term prognosis in patients with cerebral infarction. However, the specific targets and potential mechanisms of action remain unclear. This study aims to elucidate, for the first time, the potential targets of CHZF Capsule in treating ACI and improving long-term prognosis, thereby providing direction for future research on Traditional Chinese Medicine (TCM) compounds in ACI prevention and treatment (ClinicalTrials.gov Identifier: NCT06874140).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;The study focused on identifying unique differential proteins in ACI patients treated with CHZF Capsule. Twenty ACI patients were divided into a medication group (CHZ) and a control group (DZZ) based on CHZF Capsule administration. Mass spectrometry analysis was performed using 4D label-free proteome quantification technology to identify differential proteins between the groups. Functional enrichment analysis, including Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, was conducted to determine enrichment trends related to differential proteins. Hierarchical clustering was employed to identify relevant pathways enriched in each group based on &lt;em&gt;P&lt;/em&gt;-values from the enrichment analysis. The STRING (v.11.5) protein interactions network database was utilized to obtain differential protein interactions, corresponding target proteins, and major related pathways. Parallel reaction monitoring (PRM) was subsequently applied to validate the selected target proteins. Finally, chord diagrams and ROC curves were plotted for visualization and analysis.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Mass spectrometry analysis identified 1400 proteins, of which 1360 were quantitatively comparable. Using a P-value &lt; 0.05 and change thresholds of &gt; 1.5 for significant up-regulation and &lt; 1/1.5 for significant down-regulation, 63 differential proteins were identified (27 upregulated and 36 downregulated). Cluster analysis revealed that Q4 (&gt;2.0) was most closely associated with the complement and coagulation cascade pathway (hsp04610). Six proteins involved in acute inflammatory responses and immune processes were validated through PRM experiments: Complement factor H-related protein (CFHR) 4, Mannose-binding lectin protein (MBL) 2, Orosomucoid (ORM) 1, ORM2, Vascular non-inflammatory factor (VNN) 1, and Human leukocyte antigen (HLA) -A. Chord plots and ROC curve analysis suggested the following order of biomarker sensitivity: CFHR4 &gt; MBL2 &gt; VNN1 &gt; ORM1 = ORM2 &gt; HLA-A.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The observed changes in these six proteins in ACI pati","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"145 ","pages":"Article 156955"},"PeriodicalIF":6.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Protective effect of alizarin on vascular endothelial dysfunction via inhibiting the type 2 diabetes-induced synthesis of THBS1 and activating the AMPK signaling pathway” [Phytomedicine 128 (2024) 155557]","authors":"Mo-Li Zhu ,&nbsp;Jia-Xin Fan ,&nbsp;Ya-Qi Guo ,&nbsp;Li-Juan Guo ,&nbsp;Hua-Dong Que ,&nbsp;Bao-Yue Cui ,&nbsp;Yin-Lan Li ,&nbsp;Shuang Guo ,&nbsp;Ming-Xiang Zhang ,&nbsp;Ya-Ling Yin ,&nbsp;Peng Li","doi":"10.1016/j.phymed.2025.156933","DOIUrl":"10.1016/j.phymed.2025.156933","url":null,"abstract":"","PeriodicalId":20212,"journal":{"name":"Phytomedicine","volume":"144 ","pages":"Article 156933"},"PeriodicalIF":6.7,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}