Plant Root最新文献_第7页

An anatomical study of developmental changes in maturing root tissues of pomegranate (Punica granatum L.) and formation of a unique type of periderm 石榴成熟根组织发育变化及独特类型表皮形成的解剖学研究

IF 0.6

Plant Root Pub Date : 2014-01-01 DOI: 10.3117/PLANTROOT.8.33

A. Tuladhar, N. Nii

{"title":"An anatomical study of developmental changes in maturing root tissues of pomegranate (Punica granatum L.) and formation of a unique type of periderm","authors":"A. Tuladhar, N. Nii","doi":"10.3117/PLANTROOT.8.33","DOIUrl":"https://doi.org/10.3117/PLANTROOT.8.33","url":null,"abstract":"Roots of pomegranate (Punica granatum L.), belonging to Punicaceae, were investigated anatomically to record changes in tissue development from growth to maturity. When roots start secondary growth, a protective tissue called polyderm composed of alternating suberized and non-suberized cell layers, develop beyond the endodermis in certain families of plants including Myrtaceae. Punicaceae family is not known to develop a polyderm. However, new layers formed beyond the endodermis during secondary growth and biopolymer deposition was observed in their cell walls. The present study aims to gather more knowledge on this tissue discovered in pomegranate roots and cross check whether it is a polyderm or a unique type of periderm. Root specimens were sectioned freehand or with an ultramicrotome after embedding in Technovit 7100 resin. After staining with berberine hemisulfate-aniline blue-safranin O, the root sections were observed under fluorescent or optical microscopes. Unlike the polyderm in Myrtaceae roots, in pomegranate roots, ligno-suberic material accumulated in every cell layer beyond the endodermis. The alternating suberized and non-suberized layers that define the polyderm were absent. Lignin accumulation in the cell wall was pronounced in every cell layer of this outermost tissue and suberin-like autofluorescence was also observed in the same layer. We considered this to be a unique feature typical in pomegranate periderm. It is possible that accumulating both lignin and suberin in the same cell layer instead of alternative layers is more efficient because metabolic energy is not spent in forming a separate cell layer. Further experiments are underway to acknowledge changes in such biopolymer accumulation in the outermost tissue in abiotic stress conditions.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.8.33","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formation of exclusive pattern during accumulation of ligno-suberic material in cell wall of Myrtaceae root tissues including epidermis, exodermis, endodermis and polyderm 桃金桃科根组织(包括表皮、外表皮、内表皮和多表皮)细胞壁木质亚质物质积累过程中形成的排他模式

IF 0.6

Plant Root Pub Date : 2014-01-01 DOI: 10.3117/PLANTROOT.8.24

A. Tuladhar, S. Ohtsuka, N. Nii

{"title":"Formation of exclusive pattern during accumulation of ligno-suberic material in cell wall of Myrtaceae root tissues including epidermis, exodermis, endodermis and polyderm","authors":"A. Tuladhar, S. Ohtsuka, N. Nii","doi":"10.3117/PLANTROOT.8.24","DOIUrl":"https://doi.org/10.3117/PLANTROOT.8.24","url":null,"abstract":"Details on localized deposition of biopolymers in cell wall have rarely been studied. Anatomical observation is important to understand tissue-specific accumulation of biopolymers and such knowledge can further clarify tissue-function. Myrtaceae roots possess alternating suberized and non-suberized cell layers known as polyderm that exist beyond the endodermis. It is important to understand firstly, where biopolymers accumulate in this tissue and secondly what mechanisms control cell wall modification. This study aims to study areas of biopolymer deposition in Myrtaceae root tissues. Root specimens were sectioned freehand or with an ultramicrotome after embedding in Technovit 7100 resin. Root sections were stained with berberine hemisulfate-aniline blue-safranin O series or just phloroglucinol and observed under a fluorescent or optical microscope. Different biopolymers accumulated alternatively on opposite sides of the cell wall in polyderm. In non-suberized tissues, lignin accumulation was dominant and its accumulation appeared to be \"closed\" centripetally resembling the letter \"w\". In suberized tissues, it resembled the letter \"m\". This tissue-specific accumulation pattern was common in all five Myrtaceae species. What factors could control and regulate such patterned and tissue-specific accumulation of biopolymers? Could this accumulation pattern itself be a contributing factor to its protective role? Discovery of this pattern, specific to Myrtaceae root polyderm, triggers more investigations on the effect of biotic and abiotic stress on biopolymer accumulation in it.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.8.24","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Functional genetic analysis of Arabidopsis thaliana SYNC1 in Lotus corniculatus super-growing roots using the FOX gene-hunting system 利用FOX基因搜索系统对拟南芥SYNC1在莲藕超长根系中的功能遗传分析

IF 0.6

Plant Root Pub Date : 2014-01-01 DOI: 10.3117/PLANTROOT.9.6

Tsubasa Yano, Hidenori Tanaka, Taiki Kurino, A. Yamamoto, H. Kunitake, Y. Saeki, R. Akashi

引用次数: 2

Growth promoting effect of L-β-phenyllactic acid on rice (Oryza sativa L.) seedling grown under polyethylene glycol (PEG)-induced water deficit condition L-β-苯乳酸对聚乙二醇(PEG)诱导水分亏缺条件下水稻幼苗生长的促进作用

IF 0.6

Plant Root Pub Date : 2014-01-01 DOI: 10.3117/PLANTROOT.8.64

Y. Adachi, K. Kimura, M. Saigusa, T. Ohyama, Yoshihiko Takahashi, Hajime Watanabe

{"title":"Growth promoting effect of L-β-phenyllactic acid on rice (Oryza sativa L.) seedling grown under polyethylene glycol (PEG)-induced water deficit condition","authors":"Y. Adachi, K. Kimura, M. Saigusa, T. Ohyama, Yoshihiko Takahashi, Hajime Watanabe","doi":"10.3117/PLANTROOT.8.64","DOIUrl":"https://doi.org/10.3117/PLANTROOT.8.64","url":null,"abstract":"Water stress is a major limiting factor for plant growth and development. In this study, we investigated the effects of L-β-phenyllactic acid (LPA) on growth of rice (Oryza sativa L.) seedlings under polyethylene glycol (PEG)- induced water deficit conditions. Seedlings were culture at 30°C for 14 days in growth pouches supplemented with 1/100-strength Murashige and Skoog (MS) medium and PEG in the presence or absence of 100 mg L -1 LPA. As evidenced by plant height, LPA application enhanced seedling growth under PEG-induced water deficit by 13%. The shoot dry weight was slightly increased, whereas that of roots was markedly enhanced during LPA treatment by 26% under water-deficit conditions. No difference was observed among treatments in the number of roots per seedling. The ratio of shoot dry weight to shoot length (RWL) was constant regardless of treatment, indicating that LPA does not cause spindly shoot growth. The total length, surface area, and volume of fine roots were increased by LPA under PEG-induced water deficit conditions. Plant height was significantly correlated with total root surface area and volume. The results imply that PEG-induced water deficit in rice seedlings can be alleviated by LPA application. This alleviative effect is partially attributable to alterations in root system developmental patterns, with increases in fine root total length, surface, and volume accelerating water and nutrient acquisition from the culture medium.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Expression analysis of new Metallothionein2-like protein under mercury stress in tomato seedling 汞胁迫下新的金属硫蛋白样蛋白在番茄幼苗中的表达分析

IF 0.6

Plant Root Pub Date : 2014-01-01 DOI: 10.3117/PLANTROOT.8.72

Takeshi Nagata

{"title":"Expression analysis of new Metallothionein2-like protein under mercury stress in tomato seedling","authors":"Takeshi Nagata","doi":"10.3117/PLANTROOT.8.72","DOIUrl":"https://doi.org/10.3117/PLANTROOT.8.72","url":null,"abstract":"Plants including tomato produce several kinds of chelator proteins such as metallothioneins (MTs) for protection against Hg 2+ toxicity. However, the mechanism of protection from Hg 2+ is not perfectly clear. Hg 2+ content subsequently was plateaued from days 1 to 7. Cell death and DNA digestion were not observed in the primary root in the presence of Hg 2+ over the 7 days. The predicted protein sequences of 5 tomato type 2 MT-like (MT2-like) proteins were compared. The coding sequences of accession number Z68185 had no Cys-Cys motif in the N-terminal. However, the Z68185 cDNA genetic recombinant showed high resistance to Hg 2+ in bacteria. In tomato, the expression was observed in the roots, but not in the leaves or stems. mRNA of the MT2-like protein was measured in tomato seedlings exposed to 1 μM Hg 2+ . The expression level did not increase until day 3, but increased expression was observed after day 5. These results suggest that new Metallothionein2-like protein express in root specific and it may trap mercury. Our results indicate that functional identification of an MT2-like protein will be useful for molecular breeding designed to improve plant tolerance to Hg 2+ .","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.8.72","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Prospects of Plumbago rosea L. hairy root culture in traditional preparations: a phytochemical comparison with tuberous roots 传统制剂中紫花苜蓿毛状根培养的前景:与块根的植物化学比较

IF 0.6

Plant Root Pub Date : 2014-01-01 DOI: 10.3117/PLANTROOT.8.13

K. Satheeshkumar, B. Jose, Dhanya B. Pillai, P. Krishnan

{"title":"Prospects of Plumbago rosea L. hairy root culture in traditional preparations: a phytochemical comparison with tuberous roots","authors":"K. Satheeshkumar, B. Jose, Dhanya B. Pillai, P. Krishnan","doi":"10.3117/PLANTROOT.8.13","DOIUrl":"https://doi.org/10.3117/PLANTROOT.8.13","url":null,"abstract":"Agrobacterium rhizogenes mediated hairy root culture of Plumbago rosea L. is an attractive alternative for the production of plumbagin which is the major bioactive compound in P. rosea tuberous roots. The traditional industries form the major consumer of the tuberous roots as these are used in many ayurvedic preparations. The present work investigates the prospects of utilizing hairy roots in the place of tuberous roots based on bacterial survival test of hairy roots and comparison through phytochemical analyses (TLC, Spectrophotometry, HPLC and LC-MS). Since the traditional system of medicine follows stringent curing procedure before incorporation of the roots in medicinal preparations, cured tuberous and hairy roots were also compared. The phytochemical profile of hairy roots was remarkably similar to that of tuberous roots. Curing caused no change in the phytotchemical composition of the roots but only a reduction in the amount of plumbagin and other molecules. Plumbagin was reduced to 0.372 ± 0.026% dry weight (DW) in cured tuberous roots (1.15 ± 0.08% DW in uncured) and 0.061± 0.0043% DW in cured hairy roots (1.32 ± 0.09% DW in uncured). An 11.3 fold increase in root biomass with 1.56% DW plumbagin obtained in bioreactor as against 5.39 fold in shake-flasks (with 1% w/v inoculum over 3 weeks period), adds to the prospects of its applicability in traditional systems. The results suggest a refurbishment of conventional high quantity cured roots in traditional preparations with low quantity uncured roots, irrespective of root types.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Growth of 'M9' apple root in five Central Europe replanted soils “M9”苹果根在五个中欧再植土壤中的生长

IF 0.6

Plant Root Pub Date : 2014-01-01 DOI: 10.3117/PLANTROOT.8.55

S. Polverigiani, M. Kelderer, D. Neri

{"title":"Growth of 'M9' apple root in five Central Europe replanted soils","authors":"S. Polverigiani, M. Kelderer, D. Neri","doi":"10.3117/PLANTROOT.8.55","DOIUrl":"https://doi.org/10.3117/PLANTROOT.8.55","url":null,"abstract":"Soil sickness is a widespread problem in replanted apple orchards with a complex symptomatology and etiology influenced by soil and climate conditions. Consequently, a conclusive technical solution is still lacking for intensive apple orchards. The present work aims to analyze the morphological and functional changes occurring in the M9 apple root systems growing in pot filled with soil derived from five different European growing areas. For each growing area, the soil was collected from the apple orchard and used directly in the pot or gamma-ray sterilized before potting. Soil from a neighborhood fallow was also used as control for each growing area. In the non-sterilized replant soils plants developed poor root systems due to a limited biomass allocation. Fibrous roots production was particularly compromised. The roots had a smaller diameter and a lower ramification index. The root cell membrane integrity was also lower. Gamma-ray sterilized replant soils increased root growth, branching and cell integrity, while nearby fallow soil induced an intermediate root behavior. The magnitude of the symptoms showed a significant interaction between soil treatment and sampling site and root growth was correlated with the organic matter content in the soils.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.8.55","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Overexpression of CsDFR and CsANR enhanced flavonoids accumulation and antioxidant potential of roots in tobacco CsDFR和CsANR的过表达增强了烟草根中黄酮类化合物的积累和抗氧化能力

IF 0.6

Plant Root Pub Date : 2013-01-01 DOI: 10.3117/PLANTROOT.7.65

Vinay Kumar, S. Yadav

{"title":"Overexpression of CsDFR and CsANR enhanced flavonoids accumulation and antioxidant potential of roots in tobacco","authors":"Vinay Kumar, S. Yadav","doi":"10.3117/PLANTROOT.7.65","DOIUrl":"https://doi.org/10.3117/PLANTROOT.7.65","url":null,"abstract":"Flavonoids are widespread throughout the plant kingdom and present in different parts of plants. Tea (Camellia sinensis) is well known for very high content of flavonoids especially flavan-3-ols antioxidants and is an aluminium (Al) accumulator plant. Dihydroflavonol 4-reductase (DFR) and anthocyanidin reductase (ANR) are known to be regulatory enzymes of flavonoid biosynthetic pathway. In this study, cDNA encoding DFR (CsDFR) and ANR (CsANR) from tea were overexpressed individually in tobacco to check their influence on accumulation of flavonoid contents and antioxidant potential in roots of transgenic tobacco. Root morphological features, such as total volume and the number of lateral roots were improved in CsDFR and CsANR overexpressing tobacco plants relative to control tobacco plants. Both types of transgenic showed higher content of flavonoids and proanthocyanidins and lower content of anthocyanins in the roots compared to roots of control tobacco. Among flavan-3-ols, only epigallocatechin was observed in the roots and its content was higher in CsDFR and CsANR overexpressing tobacco as compared to control tobacco. Expression of genes encoding various other enzymes of flavonoid pathway like Phenylalanine ammonia-lyase, Chalcone isomerase, Flavanol synthase and Anthocyanin synthase was increased in roots of CsDFR and CsANR overexpressing tobacco plants as compared to control tobacco. The antioxidant potential of root portion of CsDFR and CsANR transgenic tobacco plants was found to be increased as indicated by enhanced total free radical scavenging activity and tolerance against Al toxicity. Taken together, these changes in roots of CsDFR and CsANR transgenic tobacco provided tolerance to aluminum toxicity.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Response of microbial respiration from fine root litter decomposition to root water content in a temperate broad-leaved forest 温带阔叶林细根凋落物分解微生物呼吸对根含水量的响应

IF 0.6

Plant Root Pub Date : 2013-01-01 DOI: 10.3117/PLANTROOT.7.77

Ayumi Kawamura, N. Makita, A. Osawa

{"title":"Response of microbial respiration from fine root litter decomposition to root water content in a temperate broad-leaved forest","authors":"Ayumi Kawamura, N. Makita, A. Osawa","doi":"10.3117/PLANTROOT.7.77","DOIUrl":"https://doi.org/10.3117/PLANTROOT.7.77","url":null,"abstract":"Microbial respiration from plant litter decomposition is sensitive to soil water status; however, its response to water status remains ambiguous, particularly in the litter of fine roots. We investigated the effect of fine-root water content on microbial respiration after 468 days of decomposition in forest soil for two diameter classes (0-0.5 and 0.5-2 mm) of Quercus serrata and Ilex pedunculosa in central Japan. Direct measurement of microbial respiration from root litter resulted in a range of 0.015-3.52 nmol CO2 g −1 s −1 . Microbial respiration in both diameter classes and species decreased linearly with decreasing root water content. These changing patterns of microbial respiration did not differ significantly between the diameter classes of either species, indicating that microbial respiration was regulated by the moisture of root litter, and not by characteristics associated with diameter class or species. In contrast, the carbon to nitrogen ratio and mass loss of the root litter differed significantly between diameter classes in both species. These findings suggest that along with chemical and morphological properties of fine root litter, the changes in root water content should also be considered as a viable factor in microbial activity variations. Drying-wetting cycles of fine roots could lead to sensitive responses of microorganisms during the short term, leading to variation in the decomposition rate of fine root litter over the long term. This study provided insight into the potential impact of microbial physiological performance on heterotrophic respiration and fine root decomposition under the varying root water content.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Auxin biology in roots 根中的生长素生物学

IF 0.6

Plant Root Pub Date : 2013-01-01 DOI: 10.3117/PLANTROOT.7.49

Hidenori Takahashi

{"title":"Auxin biology in roots","authors":"Hidenori Takahashi","doi":"10.3117/PLANTROOT.7.49","DOIUrl":"https://doi.org/10.3117/PLANTROOT.7.49","url":null,"abstract":"Auxin regulates almost every aspect of plant growth and development. Its intracellular concentration is controlled by biosynthesis and degradation. In addition, there is an \"auxin pool\" that consists of the conjugates with sugars, amino acids, and peptides. Some of the conjugates reversely release auxin, enabling alternative methods to regulate auxin concentrations. Auxin concentrations are also affected by transport. Besides the long distance delivery through the phloem, auxin is transported across the cell by influx and efflux carriers, from the shoot to root with maximum concentration at the root tip. At the root tip, the auxin flow reverses, and shootward auxin transport occurs. An auxin gradient formed this way is indispensable for proper development, maintenance of the meristem, and cell identity. The formation of root hairs is auxin-dependent. Auxin controls not only the initiation of root hairs but also regulates their elongation. In Arabidopsis thaliana, auxin accumulates in atrichoblasts and it is supplied to trichoblasts. Before hair initiation, randomization of cortical microtubule arrays is observed in lettuce seedlings. This action is promoted by auxin and is indispensable to hair formation. Furthermore, light promotes CMT randomization and root hair initiation via auxin signaling. Ethylene is another promoter of root hair formation. Ethylene affects auxin signaling and vice versa. Interactions between these hormones are synergistic for root growth inhibition but antagonistic for lateral root formation. Reactive oxygen species also regulate various responses in plants. They play an important role during root hair elongation, although their precise relationship with auxin is yet not clear.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.7.49","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69622204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24