Expression analysis of new Metallothionein2-like protein under mercury stress in tomato seedling

IF 1 Q3 PLANT SCIENCES

Plant Root Pub Date : 2014-01-01 DOI:10.3117/PLANTROOT.8.72

Takeshi Nagata

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引用次数: 5

Abstract

Plants including tomato produce several kinds of chelator proteins such as metallothioneins (MTs) for protection against Hg 2+ toxicity. However, the mechanism of protection from Hg 2+ is not perfectly clear. Hg 2+ content subsequently was plateaued from days 1 to 7. Cell death and DNA digestion were not observed in the primary root in the presence of Hg 2+ over the 7 days. The predicted protein sequences of 5 tomato type 2 MT-like (MT2-like) proteins were compared. The coding sequences of accession number Z68185 had no Cys-Cys motif in the N-terminal. However, the Z68185 cDNA genetic recombinant showed high resistance to Hg 2+ in bacteria. In tomato, the expression was observed in the roots, but not in the leaves or stems. mRNA of the MT2-like protein was measured in tomato seedlings exposed to 1 μM Hg 2+ . The expression level did not increase until day 3, but increased expression was observed after day 5. These results suggest that new Metallothionein2-like protein express in root specific and it may trap mercury. Our results indicate that functional identification of an MT2-like protein will be useful for molecular breeding designed to improve plant tolerance to Hg 2+ .

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汞胁迫下新的金属硫蛋白样蛋白在番茄幼苗中的表达分析

包括番茄在内的植物产生多种螯合蛋白，如金属硫蛋白(MTs)，以保护植物免受Hg 2+的毒害。然而，对Hg 2+的保护机制尚不完全清楚。Hg 2+含量随后在第1 ~ 7天趋于稳定。在Hg 2+存在的7天内，未观察到主根细胞死亡和DNA消化。比较了5个番茄2型mt2样蛋白的预测蛋白序列。加入号为Z68185的编码序列在n端不存在Cys-Cys基序。而重组基因Z68185在细菌中表现出对Hg 2+的高抗性。在番茄中，在根中观察到这种表达，但在叶和茎中没有。在暴露于1 μM Hg 2+的番茄幼苗中检测mt2样蛋白mRNA的表达。直到第3天，表达量没有增加，但第5天后表达量增加。这些结果表明，新的金属硫蛋白样蛋白在根特异性表达，并可能捕获汞。我们的研究结果表明，mt2样蛋白的功能鉴定将有助于提高植物对Hg 2+的耐受性的分子育种。

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来源期刊

Plant Root PLANT SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.