利用FOX基因搜索系统对拟南芥SYNC1在莲藕超长根系中的功能遗传分析

IF 1 Q3 PLANT SCIENCES

Plant Root Pub Date : 2014-01-01 DOI:10.3117/PLANTROOT.9.6

Tsubasa Yano, Hidenori Tanaka, Taiki Kurino, A. Yamamoto, H. Kunitake, Y. Saeki, R. Akashi

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引用次数: 2

摘要

为了分析拟南芥天冬酰胺- trna合成酶基因SYNC1的功能，采用豆科植物莲花超生长根(SR)猎狐系统。其中一个转化系FSL#121与亲本SR和含有空载体(对照)的SR系进行了比较，所有这些都是在蛭石培养皿中生长的。FSL#121的几种游离氨基酸水平均高于sr。同时，FSL#121具有明显的茎长、茎粗和鲜重大于sr的表型，且根长、根粗和鲜重均大于sr。此外，FSL#121的根瘤数越多，单株固氮活性越强。因此SYNC1过表达导致植物生长发生明显变化，根瘤数量增加，可能与游离氨基酸尤其是天冬酰胺含量增加有关。

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Functional genetic analysis of Arabidopsis thaliana SYNC1 in Lotus corniculatus super-growing roots using the FOX gene-hunting system

To analyze the function of SYNC1, an Arabidopsis asparaginyl-tRNA synthetase gene, the FOX-hunting system using super-growing roots (SR) from the legume species Lotus corniculatus was employed. One transformed line, FSL#121, was compared to parental SR and to an SR line harboring the empty vector (Control), all of which were grown in vivo using vermiculite pots. The level of several free amino acids was higher in FSL#121 than SR. Concomitantly, FSL#121 had a distinct phenotype of greater shoot length, stem diameter and shoot fresh weight compared with SR. Also, the root length, root diameter and fresh root weight were greater in FSL#121 than SR. Furthermore, the greater number of nodules in FSL#121 increased the nitrogen fixation activity per whole plant. Therefore, SYNC1 overexpression caused distinct changes in plant growth, increased the number of root nodules, and may be involved in increasing the amount of free amino acids, especially asparagine.

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来源期刊

Plant Root PLANT SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.