Plant Root最新文献_第4页

Cumulative effect of thidiazuron and 1-naphthylacetic acid in massive root proliferation of micropropagated sugarcane plantlet 硫脲和1-萘乙酸对甘蔗微繁植株块根增殖的累积效应

IF 0.6

Plant Root Pub Date : 2018-01-01 DOI: 10.3117/PLANTROOT.12.16

K. Kumari, M. Lal, S. Saxena

{"title":"Cumulative effect of thidiazuron and 1-naphthylacetic acid in massive root proliferation of micropropagated sugarcane plantlet","authors":"K. Kumari, M. Lal, S. Saxena","doi":"10.3117/PLANTROOT.12.16","DOIUrl":"https://doi.org/10.3117/PLANTROOT.12.16","url":null,"abstract":": Thidiazuron (TDZ), a well known plant growth regulator is used in tissues culture as a sup-plement to the basal MS medium. Several reports suggest the role of TDZ in promoting plant organogenesis and plant regeneration. Our experiments to micropropagate the sugarcane using various growth regulators reveal the role of TDZ in rooting and survival of a high yielding new sugarcane variety Co 05011. Various permutation combinations were tried using TDZ as the main regulator to rapidly in-crease the number of plantlets. Shoot cultures were repeatedly multiplied for 10 cycles and then transferred to MS medium augmented with various concentration of TDZ along with 1-naphthylacetic acid (NAA) and sucrose for rooting. Results of present study indicate optimum concentration of TDZ (0.002 mg/L) in combination with NAA (0.5 mg/L) resulted in 93.6% root formation. Moreover, when these shoots were re-cultured in the same media for an-other two cycles it produced 100% rooting and had almost 100% survival during acclimatization. These results indicate that TDZ and NAA combination enhance the activity of NAA and resulting in massive proliferation of roots. TDZ increases the frequency and proliferation of healthy and vigorous roots in micropropagated shoots thus enhancing their survival rate in field and during hardening process. This work can be helpful in developing successful and cost effective tissue culture of elite sugarcane varieties.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"12 1","pages":"16-20"},"PeriodicalIF":0.6,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification of promoter for adventitious root-specific gene expression from sweet potato 甘薯不定根特异性基因表达启动子的鉴定

IF 0.6

Plant Root Pub Date : 2018-01-01 DOI: 10.3117/PLANTROOT.12.31

N. Tanabe, Akane Ito, M. Tamoi, S. Shigeoka

{"title":"Identification of promoter for adventitious root-specific gene expression from sweet potato","authors":"N. Tanabe, Akane Ito, M. Tamoi, S. Shigeoka","doi":"10.3117/PLANTROOT.12.31","DOIUrl":"https://doi.org/10.3117/PLANTROOT.12.31","url":null,"abstract":"Since sweet potato (Ipomoea batatas) produces high yield of storage roots (SR), it is an attractive target for improving productivity. However, molecular information about sweet potato is limited. The number of SR per plants determines the yield of sweet potato. SRs develop from adventitious roots (AR). Therefore, the mechanisms responsible for the initiation and development of AR need to be elucidated to increase the productivity of sweet potato. We conducted a transcriptomic analysis between nodes containing AR primordia (ARnode) and stems using next-generation sequencing to identify AR-specific promoters. A total of 6,219 contigs exhibited stronger expression in the ARnodes than in the stems. Among them, we found that the expression of sweet potato Plant AT-rich sequenceand zinc-binding protein (IbPLATZ) transcription factors was AR-specific. We examined the promoter activity of IbPLATZ in the transgenic Arabidopsis plants. β-glucuronidase (GUS) staining showed that the IbPLATZ promoter conferred the expression of the GUS reporter gene in a root tipspecific manner. These results indicate that the IbPLATZ promoter is available for a root tip-specific foreign gene expression system in transgenic plants.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"119 1","pages":"31-44"},"PeriodicalIF":0.6,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.12.31","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Galactose oxidase/kelch repeat-containing protein is involved in the iron deficiency stress response in the roots of Hyoscyamus albus 半乳糖氧化酶/含kelch重复蛋白参与了水仙根系缺铁胁迫反应

IF 0.6

Plant Root Pub Date : 2017-11-30 DOI: 10.3117/PLANTROOT.11.58

Yuki Kawahara, T. Hashimoto, H. Nakayama, Y. Kitamura

引用次数: 6

A scanner-based rhizobox system enabling the quantification of root system development and response of Brassica rapa seedlings to external P availability 一种基于扫描仪的根箱系统，能够量化油菜幼苗的根系发育和对外部磷有效性的反应

IF 0.6

Plant Root Pub Date : 2017-05-16 DOI: 10.3117/PLANTROOT.11.16

M. O. Adu, D. Yawson, M. Bennett, M. Broadley, L. Dupuy, P. White

{"title":"A scanner-based rhizobox system enabling the quantification of root system development and response of Brassica rapa seedlings to external P availability","authors":"M. O. Adu, D. Yawson, M. Bennett, M. Broadley, L. Dupuy, P. White","doi":"10.3117/PLANTROOT.11.16","DOIUrl":"https://doi.org/10.3117/PLANTROOT.11.16","url":null,"abstract":"Rhizoboxes are soil-root compartments that may well provide the closest naturalistic conditions for studying root systems architectures (RSAs) in controlled environments. Rhizobox-based studies can however lead to mis-estimation of root traits due to poor recovery of roots and loss of fine root features during washing of roots. We used a novel scanner-based rhizobox system to evaluate: (i) RSA traits of Brassica rapa genotypes; (ii) the relationship between root traits recorded from rhizoboxes and those of harvested roots and (iii) genotypic variation of seedlings in response to external P ([P] ext) availability. Brassica rapa genotypes were grown in soil-filled rhizoboxes abutting flatbed scanners and were watered once with either deionised water or a solution of 600 μM KH2PO4 to approximately 80% field capacity on a weight basis. Shoot and root P concentrations ([P]shoot and [P]root) of the B. rapa lines grown on different [P]ext were quantified. Visible root length at the surface of rhizoboxes constituted 85% of the total root length recovered from harvested root samples. High P supply induced a strong increase in [P]shoot in all genotypes (P < 0.001) whereas low P supply generally led to greater partitioning to roots. Seed P concentration and tissue P concentration were correlated only at low [P]ext. Total root length was strongly correlated with tissue P content under both low [P]ext (r = 0.81, P < 0.05) and high [P]ext (r = 0.82, P < 0.05) conditions. The novel scanner-based rhizobox system used addresses the substantial limitations associated with current use of rhizoboxes to study root growth dynamics.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"11 1","pages":"16-32"},"PeriodicalIF":0.6,"publicationDate":"2017-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.11.16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42459970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Aseptic germination and Agrobacterium rhizogenes-mediated transformation of Taraxacum kok-saghyz Rodin 蒲公英的无菌萌发及根源农杆菌介导的转化

IF 0.6

Plant Root Pub Date : 2017-01-01 DOI: 10.3117/PLANTROOT.11.64

A. Knyazev, B. Kuluev, E. Mikhaylova, Gulnar Yasybaeva, A. Chemeris

引用次数: 2

Promotion of root elongation by pyridinecarboxylic acids known as novel cut flower care agents 新型切花护理剂吡啶羧酸促进根系伸长

IF 0.6

Plant Root Pub Date : 2017-01-01 DOI: 10.3117/PLANTROOT.11.40

S. Satoh, Yoshihiro Nomura

{"title":"Promotion of root elongation by pyridinecarboxylic acids known as novel cut flower care agents","authors":"S. Satoh, Yoshihiro Nomura","doi":"10.3117/PLANTROOT.11.40","DOIUrl":"https://doi.org/10.3117/PLANTROOT.11.40","url":null,"abstract":"Pyridinedicarboxylic acid (PDCA) analogs, including 2,3-, 2,4-, 2,5-, 2,6-, 3,4and 3,5PDCA, accelerate flower opening and retard senescence of spray-type carnation flowers. In addition the present study revealed that 2,3-PDCA promoted root elongation in lettuce, carrot and rice seedlings, whereas 2,4-PDCA inhibited it. Then, the action of PDCA and pyridinecarboxylic acid (PCA) analogs on root elongation was further examined using rice seedlings. 2,3-, 3,4and 3,5PDCA promoted rice root elongation, whereas 2,4and 2,6-PDCA inhibited, and 2,5-PDCA had little effect. 3-PCA (nicotinic acid) promoted rice root elongation, but 2and 4-PCA did not. Moreover, 3-PCA amide (nicotinamide) did not promote root elongation. These findings indicated that a carboxyl group substituted on position 3 of the pyridine ring is necessary to promote root elongation, and that the promoting effect of 3-PCA was not from its action as vitamin B3, but from its intrinsic activity as a 3-COOH substituted pyridine. On the other hand, all the PCA and PDCA analogs tested in this study, except 2,6-PDCA and 4-PCA, promoted shoot elongation of rice seedlings.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"198 1","pages":"40-47"},"PeriodicalIF":0.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.11.40","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69620959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Biolistic-mediated plasmid-free transformation for induction of hairy roots in tobacco plants 生物介导的无质粒转化诱导烟草毛状根

IF 0.6

Plant Root Pub Date : 2017-01-01 DOI: 10.3117/PLANTROOT.11.33

Gulnar Yasybaeva, Z. Vershinina, B. Kuluev, E. Mikhaylova, A. Baymiev, A. Chemeris

引用次数: 5

Compensatory growth of lateral roots responding to excision of seminal root tip in rice 水稻精根尖切除后侧根的补偿性生长

IF 0.6

Plant Root Pub Date : 2017-01-01 DOI: 10.3117/PLANTROOT.11.48

Tsubasa Kawai, Misuzu Nosaka-Takahashi, A. Yamauchi, Y. Inukai

引用次数: 10

Quantitative evaluation of plastic root responses to contiguous water gradient in rice 水稻塑性根对连续水分梯度响应的定量评价

IF 0.6

Plant Root Pub Date : 2017-01-01 DOI: 10.3117/PLANTROOT.11.70

Mana Kano‐Nakata, Y. Inukai, J. Siopongco, Shiro Mitsuya, A. Yamauchi

{"title":"Quantitative evaluation of plastic root responses to contiguous water gradient in rice","authors":"Mana Kano‐Nakata, Y. Inukai, J. Siopongco, Shiro Mitsuya, A. Yamauchi","doi":"10.3117/PLANTROOT.11.70","DOIUrl":"https://doi.org/10.3117/PLANTROOT.11.70","url":null,"abstract":"Root plasticity is the key trait for plant adaptation to environmental stresses. To quantify phenotypic plasticity to its full extent in potential, it needs to be evaluated under contiguous environmental changes. For that purpose, we used the combination of chromosome segment substitution lines (Nipponbare/Kasalath CSSLs) and line source sprinkler (LSS) system of irrigation. For analysis, we first attempted to apply the coefficient of variation (CV) and norm of reaction that have been used as the conventional approaches, and then propose a new approach for quantification of root plasticity. Results revealed that CV was not linked to root plasticity under contiguous water gradient in this study. In contrast, norm of reaction was linked to root plasticity and better explained with curve than linear, especially for CSSL50 (the most plastic genotypes) under such gradient. Based on the norm of reaction with curve, root plasticity was calculated using the difference in total root length between CSSLs and the recurrent parent, Nipponbare. Further analysis of root plasticity in relation to dry matter production was also done. By applying the new approach, we confirmed that root plasticity expression was affected by the intensities of drought stress and genotypes, indicating that such root plasticity is genetically controlled. In addition, root plasticity effectively contributed to the dry matter production under the drought conditions and maximized at around 20% of soil moisture content (–0.04 MPa). By using CSSLs and LSS system, we successfully evaluated root plasticity under contiguous water gradient.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"11 1","pages":"70-78"},"PeriodicalIF":0.6,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3117/PLANTROOT.11.70","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69620995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Lateral root elongation inside the basal cortex of the seminal root is caused by the changes in auxin distribution to the root system of rice (Oryza sativa L.) seedlings 水稻(Oryza sativa L.)幼苗根系生长素分布的变化导致了种子根基部皮层内侧根伸长

IF 0.6

Plant Root Pub Date : 2017-01-01 DOI: 10.3117/PLANTROOT.11.10

Atsushi Ogawa, Maya Matsunami, Yusuke Suzuki, K. Toyofuku, H. Wabiko

引用次数: 1