M. O. Adu, D. Yawson, M. Bennett, M. Broadley, L. Dupuy, P. White
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引用次数: 7
摘要
根箱是土壤根系间室,可以为在受控环境下研究根系结构(RSAs)提供最接近自然的条件。然而,基于根箱的研究可能会导致对根系性状的错误估计,因为在根系洗涤过程中,根系恢复不良和细根特征的丧失。本研究采用一种新型的基于扫描仪的根箱系统来评价:(1)油菜基因型的RSA性状;(ii)根箱记录的根性状与收获根性状之间的关系;(iii)幼苗对外部磷(P [P]等)有效性的基因型变异。rapa基因型油菜生长在与平板扫描仪相邻的填土根箱中,用去离子水或600 μM KH2PO4溶液浇灌一次,以重量为基础约80%的田间容量。测定了不同[P]ext上生长的白刺系茎部和根部磷含量([P]茎部和[P]根)。根箱表面可见根长占采根样品恢复总根长的85%。在所有基因型中,高磷供应诱导了[P]茎部的强烈增加(P < 0.001),而低磷供应通常导致更大的根系分配。种子磷浓度与组织磷浓度仅在低磷水平下相关。在低[P]ext (r = 0.81, P < 0.05)和高[P]ext (r = 0.82, P < 0.05)条件下,总根长与组织磷含量呈极显著相关。新的基于扫描仪的根箱系统解决了目前使用根箱研究根系生长动态的实质性限制。
A scanner-based rhizobox system enabling the quantification of root system development and response of Brassica rapa seedlings to external P availability
Rhizoboxes are soil-root compartments that may well provide the closest naturalistic conditions for studying root systems architectures (RSAs) in controlled environments. Rhizobox-based studies can however lead to mis-estimation of root traits due to poor recovery of roots and loss of fine root features during washing of roots. We used a novel scanner-based rhizobox system to evaluate: (i) RSA traits of Brassica rapa genotypes; (ii) the relationship between root traits recorded from rhizoboxes and those of harvested roots and (iii) genotypic variation of seedlings in response to external P ([P] ext) availability. Brassica rapa genotypes were grown in soil-filled rhizoboxes abutting flatbed scanners and were watered once with either deionised water or a solution of 600 μM KH2PO4 to approximately 80% field capacity on a weight basis. Shoot and root P concentrations ([P]shoot and [P]root) of the B. rapa lines grown on different [P]ext were quantified. Visible root length at the surface of rhizoboxes constituted 85% of the total root length recovered from harvested root samples. High P supply induced a strong increase in [P]shoot in all genotypes (P < 0.001) whereas low P supply generally led to greater partitioning to roots. Seed P concentration and tissue P concentration were correlated only at low [P]ext. Total root length was strongly correlated with tissue P content under both low [P]ext (r = 0.81, P < 0.05) and high [P]ext (r = 0.82, P < 0.05) conditions. The novel scanner-based rhizobox system used addresses the substantial limitations associated with current use of rhizoboxes to study root growth dynamics.
期刊介绍:
Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.