蒲公英的无菌萌发及根源农杆菌介导的转化

IF 1 Q3 PLANT SCIENCES

Plant Root Pub Date : 2017-01-01 DOI:10.3117/PLANTROOT.11.64

A. Knyazev, B. Kuluev, E. Mikhaylova, Gulnar Yasybaeva, A. Chemeris

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引用次数: 2

摘要

Taraxacum kok-saghyz Rodin，也被称为俄罗斯蒲公英，是温带地区最有前途的天然橡胶来源。由于俄罗斯蒲公英的根中含有大量橡胶，因此开发出毛状根的培育方法和转移靶基因以改善其基因组是一个重要的研究方向。研究了不同灭菌剂对赤霞珠种子无菌萌发的影响，以及不同灭菌剂对赤霞珠种子污染和发芽率的影响。采用农杆菌介导的植物转化为不育的秋蒿。在无激素的半强度Murashige和Skoog培养基上生长毛状根。A4菌株的效率高于15834菌株。通过PCR和组织化学GUS分析证实了转化。含有报告基因GUS基因的谷子转基因植株从被解剖的毛状根中自发再生。

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Aseptic germination and Agrobacterium rhizogenes-mediated transformation of Taraxacum kok-saghyz Rodin

Taraxacum kok-saghyz Rodin, also known as Russian dandelion, is the most promising source of natural rubber in the temperate climate zone. As the roots of Russian dandelion accumulate rubber, it is of great interest to develop methods to produce hairy roots and to transfer target genes to improve its genome. We studied the aseptic germination of T. kok-saghyz seeds, using different sterilizing agents and their influence on contamination and germination rate. Sterile T. kok-saghyz plants were used for Agrobacterium rhizogenesmediated transformation. T. kok-saghyz hairy roots developed on hormone-free half-strength Murashige and Skoog medium. The A4 strain of A. rhizogenes demonstrated higher efficiency than the 15834 strain. Transformation was confirmed by PCR and histochemical GUS analysis. Transgenic plants of kok-saghyz, containing the reporter GUS gene, spontaneously regenerated from dissected hairy roots.

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来源期刊

Plant Root PLANT SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.