Placenta最新文献

{"title":"Beyond 2D: Novel biomaterial approaches for modeling the placenta","authors":"Samantha G. Zambuto ,&nbsp;Adrienne K. Scott ,&nbsp;Michelle L. Oyen","doi":"10.1016/j.placenta.2024.03.006","DOIUrl":"10.1016/j.placenta.2024.03.006","url":null,"abstract":"<div><div><span>This review considers fully three-dimensional biomaterial environments of varying complexity as these pertain to research on the placenta. The developments in placental cell sources are first considered, along with the corresponding maternal cells with which the trophoblast interact. We consider biomaterial sources, including hybrid and composite biomaterials. Properties and characterization of biomaterials are discussed in the context of material design for specific placental applications. The development of increasingly complicated three-dimensional structures includes examples of advanced fabrication methods such as microfluidic device fabrication and 3D bioprinting, as utilized in a placenta context. The review finishes with a discussion of the potential for </span><em>in vitro</em><span>, three-dimensional placenta research to address health disparities and sexual dimorphism, especially in light of the exciting recent changes in the regulatory environment for </span><em>in vitro</em> devices.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"157 ","pages":"Pages 55-66"},"PeriodicalIF":3.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140181753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental methylation and pro-inflammatory protein levels in cord blood","authors":"Sanne D. van Otterdijk ,&nbsp;Alexandra M. Binder ,&nbsp;Karin B. Michels","doi":"10.1016/j.placenta.2024.10.067","DOIUrl":"10.1016/j.placenta.2024.10.067","url":null,"abstract":"<div><h3>Introduction</h3><div>The neonates’ inflammatory response may in part be shaped during development by the placental epigenome, but evidence is scarce. We investigated the association between placental DNA methylation and pro-inflammatory proteins in cord blood.</div></div><div><h3>Methods</h3><div>A total of 249 mother-child dyads from the Harvard Epigenetic Birth Cohort were included in this study. Genome-wide methylation in placental DNA was assessed using the Illumina Human Methylation 450 Bead Chip array and verified by pyrosequencing. Cord blood inflammation markers assessed were interleukin-6, interleukin-8 and tumor necrosis factor α, intercellular adhesion molecule 1, serum amyloid A, and C-reactive protein.</div></div><div><h3>Results</h3><div>We identified differential placental DNA methylation of three loci in the <em>HIVEP3</em> gene shore region that were associated with TNFα protein levels in cord blood. TNFα levels were associated with mode of delivery, gestational age and birth order. Three other loci located in the open sea region of the <em>BCL11B</em> gene were associated with SAA protein levels in cord blood. SAA levels were associated with birthweight, gestational age, and infant sex.</div></div><div><h3>Conclusions</h3><div>Our results suggest a potential role for <em>HIVEP3</em> and <em>BCL11B</em> placental DNA methylation in the acute immune response of the neonate. These immune markers were correlated with several mother and child characteristics.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 231-239"},"PeriodicalIF":3.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis identified novel biomarker in invasive placenta accreta spectrum","authors":"Xiaoming Shi ,&nbsp;Ling Jin ,&nbsp;Xinlu Meng ,&nbsp;Xiao Huo ,&nbsp;Yan Sun ,&nbsp;Lixiang Xue ,&nbsp;Yuan Wei ,&nbsp;Yuanyuan Wang ,&nbsp;Zhongnan Yin ,&nbsp;Yangyu Zhao ,&nbsp;Lian Chen","doi":"10.1016/j.placenta.2024.10.023","DOIUrl":"10.1016/j.placenta.2024.10.023","url":null,"abstract":"<div><h3>Introduction</h3><div>Placenta accreta spectrum (PAS) disorders pose a grave threat to maternal life due to severe hemorrhage and the heightened risk of peripartum hysterectomy. Consequently, there's a pressing need for circulating biomarkers in clinical settings. MicroRNAs (miRNAs), being stable in peripheral circulation, hold promise as potential biomarkers for PAS.</div></div><div><h3>Methods</h3><div>This study recruited singleton live pregnancies, including cases of invasive PAS, placenta previa (PP), and controls, across three phases. Initially, RNA-seq of peripheral blood identified 6 miRNAs in the screening phase. Subsequently, in the training and validation phases, miR-23a-5p, along with its target genes ASF1B and CHTF8, were validated using qRT-PCR. The diagnostic value of these markers for PAS and adverse outcomes was evaluated using Receiver Operating Characteristic (ROC) curves.</div></div><div><h3>Results</h3><div>The results showed miR-23a-5p was down-regulated in PAS, whereas ASF1B and CHTF8 were up-regulated. miR-23a-5p had modest diagnostic efficiency for PAS and adverse outcomes, as the AUC were 0.689 and 0.711 respectively. However, when miR-23a-5p combined with CHTF8, the AUC can improve greatly to 0.869 in PAS diagnosis and 0.856 in prediction of adverse outcomes.</div></div><div><h3>Discussion</h3><div>We propose the miR-23a-5p plays a role in PAS pathogenesis through regulating cell proliferation, migration, invasion, apoptosis by targeting various genes. This study confirmed its potential value of miR-23a-5p combined with target gene CHTF8 as novel biomarkers for PAS and adverse outcomes.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 301-309"},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feto-placental vascular structure and in silico haemodynamics: Of mice, rats, and human","authors":"Nikhilesh Bappoo ,&nbsp;Yutthapong Tongpob ,&nbsp;Matina Hakim ,&nbsp;Jenny Myers ,&nbsp;Emma Panting ,&nbsp;Karen E. Chapman ,&nbsp;Adrian J.W. Thomson ,&nbsp;Carmel M. Moran ,&nbsp;Lachlan J. Kelsey ,&nbsp;Vijayalakshmi Srinivasan ,&nbsp;Joanna L. James ,&nbsp;Alys R. Clark ,&nbsp;Barry J. Doyle ,&nbsp;Caitlin S. Wyrwoll","doi":"10.1016/j.placenta.2024.10.020","DOIUrl":"10.1016/j.placenta.2024.10.020","url":null,"abstract":"<div><h3>Introduction</h3><div>The complex arborization of the feto-placental vasculature is crucial for optimal fetal nutrition, waste exchange and ultimately, development. Ethical and experimental limitations constrain research into the human placenta, hence experimental animal models such as mice and rats, are crucial to understand placental function. It is unclear how well the mouse and rat feto-placental vascular structure emulates human. Moreover, the implications of differences in vascular structure, especially in arborization, for placental function remain unclear.</div></div><div><h3>Methods</h3><div>We use micro-computed tomography imaging, high frequency Doppler ultrasound and computational fluid dynamics to characterize feto-placental vasculature structure and haemodynamics in mice, rats, and human.</div></div><div><h3>Results</h3><div>Our data suggest that despite structural differences between rat and mouse placenta, haemodynamics are similar and that both hold applicability to investigating feto-placental structure and function. We show that human cotyledons demonstrate vascularity-dependent haemodynamic behaviour (including flow deceleration and oxygen exchange) similar to rodents and can be analysed in the same spectrum as rodents. Finally, we show strong structure-function relationships when interspecies datasets are combined; notably, we demonstrate that surrogate measures such as vascularity, can be used to estimate placental oxygen exchange function.</div></div><div><h3>Discussion</h3><div>Pre-clinical placental research utilising rat and mouse placentae to understand the impact of feto-placental arborization on placental function and fetal development can inform the human context.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 175-184"},"PeriodicalIF":3.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylation and expression of imprinted genes in circulating extracellular vesicles from women experiencing early onset preeclampsia","authors":"Uma Shinde ,&nbsp;Kushaan Khambata ,&nbsp;Sanketa Raut ,&nbsp;Aishwarya Rao ,&nbsp;Vandana Bansal ,&nbsp;Niranjan Mayadeo ,&nbsp;Dhanjit kumar Das ,&nbsp;Taruna Madan ,&nbsp;Vinoth Prasanna Gunasekaran ,&nbsp;Nafisa Huseni Balasinor","doi":"10.1016/j.placenta.2024.10.019","DOIUrl":"10.1016/j.placenta.2024.10.019","url":null,"abstract":"<div><h3>Introduction</h3><div>Preeclampsia (PE) is a pregnancy complication marked by high blood pressure, posing risk to maternal and fetal health. \"Genomic imprinting\", an epigenetic phenomenon regulated by DNA methylation at Differently Methylated Regions (DMR's), influences placental development. Research on circulating extracellular vesicles (EVs) in PE suggests them as potential source for early biomarkers, but methylation status of EV-DNA in Preeclampsia is not reported yet.</div></div><div><h3>Methods</h3><div>This study examines the methylation and expression profile of imprinted genes - <em>PEG10</em>, <em>PEG3</em>, <em>MEST</em>, and <em>DLK1</em> in circulating EVs of 1^st and 3^rd trimester control and early onset preeclampsia (EOPE) pregnant women (n = 15) using pyrosequencing and qRT-PCR respectively.</div></div><div><h3>Results</h3><div>In 1^st trimester, <em>PEG3</em> was significantly hypermethylated, whereas no significant methylation changes were noted in <em>PEG10</em> and <em>MEST</em> in EOPE. In 3^rd trimester, significant hypomethylation in <em>PEG10</em>, <em>PEG3</em> and IGDMR was observed whereas significant hypermethyaltion noted in <em>MEST</em>. mRNA expression of <em>PEG10</em>, <em>PEG3</em> and <em>DLK1</em> was not affected in circulating EVs of 1^st trimester EOPE. However, in 3^rd trimester significant increased expression in <em>PEG10</em>, <em>PEG3</em> and <em>DLK1</em> noted. <em>MEST</em> expression was reduced in 3^rd trimester EOPE. No correlation was observed between average DNA methylation and gene expression in <em>PEG10</em> and <em>PEG3</em> in 1^st trimester. However, in 3^rd trimester, significant negative correlation was noted in <em>PEG10</em> (r = −0.426, p = 0.04), <em>PEG3</em> (r = −0.496, p = 0.01), <em>MEST</em> (r = −0.398, p = 0.03) and <em>DLK1</em> (r = −0.403, p = 0.03).</div></div><div><h3>Discussion</h3><div>The results of our study strengthen the potential of circulating EVs from maternal serum as non-invasive indicators of placental pathophysiology, including preeclampsia.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 206-215"},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-142-5p mediated Nrf2 dysregulation in gestational diabetes mellitus and its impact on placental angiogenesis","authors":"K.L. Milan ,&nbsp;V. Gayatri ,&nbsp;Kumaran Kriya ,&nbsp;N. Sanjushree ,&nbsp;Sri Vishwanathan Palanivel ,&nbsp;M. Anuradha ,&nbsp;Kunka Mohanram Ramkumar","doi":"10.1016/j.placenta.2024.10.021","DOIUrl":"10.1016/j.placenta.2024.10.021","url":null,"abstract":"<div><h3>Introduction</h3><div>Gestational diabetes mellitus (GDM) presents significant risks during pregnancy, including adverse perinatal outcomes and placental dysfunction. Impaired angiogenesis, involving crucial factors like Vascular Endothelial Growth Factor (VEGF), contributes to these complications. The Nrf2/Keap1 pathway, crucial for vascular redox homeostasis, has been linked to GDM-associated angiogenesis dysregulation.</div></div><div><h3>Methods</h3><div>This study aimed to investigate the molecular mechanisms underlying placental Nrf2 regulation, focusing on angiomiRs, key regulators of angiogenesis in GDM. Computational analysis identified miR-142-5p targeting Nrf2 mRNA. Expression levels of miR-142-5p were assessed in GDM placenta and correlated with Nrf2 expression. Experimental validation utilized human trophoblastic cell lines (BeWo) exposed to hyperglycemic conditions, assessing the effects of anti-miR-142 transfection on Nrf2 expression and angiogenic marker levels.</div></div><div><h3>Results</h3><div>miR-142-5p expression was significantly downregulated in GDM placenta, correlating positively with Nrf2 expression. In BeWo cells exposed to hyperglycemia, anti-miR-142 transfection notably increased Nrf2 expression alongside angiogenic marker levels, confirming the computational predictions.</div></div><div><h3>Discussion</h3><div>Our findings highlight the pivotal role of miRNAs in GDM-associated impaired angiogenesis by modulating Nrf2 expression. Understanding these molecular mechanisms provides insights into potential therapeutic targets for improving pregnancy outcomes in GDM cases.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 192-199"},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental volume at gestational week 27 and subsequent fetal growth: An observational study","authors":"Helene Fjeldvik Peterson ,&nbsp;Kari Flo ,&nbsp;Silje Sommerfelt ,&nbsp;Vigdis Hillestad","doi":"10.1016/j.placenta.2024.10.022","DOIUrl":"10.1016/j.placenta.2024.10.022","url":null,"abstract":"<div><h3>Objectives</h3><div>To study if placental volume and placental to fetal ratio at gestational week (GW) 27 correlate with subsequent fetal growth. We also investigated whether the 1/3 smallest and 1/3 largest fetuses have different growth potential depending on placental volume.</div></div><div><h3>Methods</h3><div>Placental and fetal volume was measured by magnetic resonance imaging (MRI) at GW 27 and 37 in 86 singleton pregnancies. Placental to fetal ratio was calculated as placental volume/fetal volume. Growth was calculated as [(fetal volume at GW 37 – fetal volume at GW 27)/number of days between the MRI examinations]. To explore whether a higher placental volume affected growth of small and large fetuses differently, we performed separate analyses of the 1/3 smallest and 1/3 largest fetuses with placental volume under and above the median at GW 27.</div></div><div><h3>Results</h3><div>We found a positive correlation of both placental volume and placental to fetal ratio at GW 27 with average growth velocity, r = 0.51 (p &lt; 0.001) and r = 0.33 (p = 0.002) respectively. The correlation between fetal volume at GW 27 and average growth velocity was r = 0.48 (p &lt; 0.001). The small fetuses had significantly lower average growth velocity if the placental volume was low compared to if the placental volume was high, 22 (SD 3) cm³/day versus 25 (SD 3) cm³/day, p = 0.02. Among the large fetuses, placental volume did not significantly affect growth.</div></div><div><h3>Conclusions</h3><div>Placental volume and placental to fetal ratio at GW 27 were positively correlated with subsequent fetal growth. Possibly, placental size is an indicator of fetal growth potential, especially among small fetuses.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 200-205"},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placenta-associated biomarkers and pregnancy outcome in HPA-1a alloimmunization: A prospective cohort study","authors":"Nora Hersoug Nedberg ,&nbsp;Mona Nystad ,&nbsp;Maria Therese Ahlen ,&nbsp;Eirin Listau Bertelsen ,&nbsp;Katarzyna Guz ,&nbsp;Małgorzata Uhrynowska ,&nbsp;Marzena Dębska ,&nbsp;Agnieszka Gierszon ,&nbsp;Agnieszka Orzińska ,&nbsp;Anne Husebekk ,&nbsp;Ewa Brojer ,&nbsp;Anne Cathrine Staff ,&nbsp;Heidi Tiller","doi":"10.1016/j.placenta.2024.10.014","DOIUrl":"10.1016/j.placenta.2024.10.014","url":null,"abstract":"<div><h3>Introduction</h3><div>Fetal and neonatal alloimmune thrombocytopenia (FNAIT) results from parental incompatibility in human platelet antigens (HPA) and subsequent maternal sensitization. The HPA-1a epitope is also expressed on placental tissue. Chronic placental inflammation and lower birth weight is observed more often in HPA-1a alloimmunized pregnancies, suggesting a placental component in the pathophysiology of FNAIT. Today, prediction of FNAIT severity is limited. The aim of the study was to investigate whether dysregulated maternal angiogenic proteins are associated with neonatal outcome in HPA-1a alloimmunized pregnancies.</div></div><div><h3>Material and methods</h3><div>Eighty-seven HPA-1a negative pregnant women were identified from a large prospective screening study in Poland (PREVFNAIT) including 43 HPA-1a immunized and 44 non-immunized controls. Placental growth factor (PlGF), soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) were measured in maternal plasma from 2nd and 3rd trimester by enzyme-linked immunosorbent assay and levels/ratios were compared between study groups, using uni- and multivariable analyses. Main outcome measures were either classic FNAIT-related (severe thrombocytopenia, petechia, intracranial hemorrhage), placenta-related (small for gestational age) or a composite variable combining them all.</div></div><div><h3>Results</h3><div>There were no significant differences in plasma concentrations of sFlt-1, PlGF, sEng nor sFlt-1/PlGF ratio when comparing immunized and non-immunized pregnancies. Among HPA-1a alloimmunized pregnancies, increasing levels of the sFlt-1 protein in 3rd trimester were significantly associated with lower neonatal platelet count (multivariable linear regression, p = 0.024). Increased sFlt-1 and sFlt-1/PlGF ratio in 3rd trimester were significantly associated with higher odds of a composite adverse neonatal outcome in alloimmunized pregnancies (multivariable logistic regression, p = 0.029 and p = 0.019, respectively).</div></div><div><h3>Conclusion</h3><div>An anti-angiogenic profile in HPA-1a alloimmunized mothers is associated with a composite adverse neonatal outcome. This suggests that sFlt-1 and the sFlt-1/PlGF ratio may assist in predelivery risk stratification and clinical management decisions for FNAIT.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 185-191"},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endothelin-1 potentiated constriction in preeclampsia placental veins: Role of ETAR/ETBR/CaV1.2/CALD1","authors":"Hongyu Su ,&nbsp;Min Li ,&nbsp;Na Li ,&nbsp;Yingying Zhang ,&nbsp;Yun He ,&nbsp;Ze Zhang ,&nbsp;Yumeng Zhang ,&nbsp;Qinqin Gao ,&nbsp;Zhice Xu ,&nbsp;Jiaqi Tang","doi":"10.1016/j.placenta.2024.10.011","DOIUrl":"10.1016/j.placenta.2024.10.011","url":null,"abstract":"<div><h3>Background</h3><div>Placenta plays a vital role in preeclampsia. The present study investigated the role of endothelin-1 (ET-1) and its receptors in preeclampsia placenta.</div></div><div><h3>Method</h3><div>Placenta samples were collected from normal and preeclampsia pregnancies, with one single fetus. Placental chorionic plate vessel tone was measured with DMT using vasoactive agents with or without antagonists. Role of L-type voltage-dependent calcium channels (CaV1.2) in single smooth muscle cell was detected using whole-cell patch clamp. PCR, Western blot, and ELISA was used to detect molecule expressions. Placental vessel explants and human umbilical vein smooth muscle cell (HUVSMC) were exposed to ET-1 treatment with or without antagonists. Human umbilical vein endothelial cell (HUVEC) and pregnant sheep was exposed to hypoxic condition, simulating preeclampsia.</div></div><div><h3>Results</h3><div>ET-1 and IRL1620 mediated stronger contractions in preeclampsia placental veins, despite unchanged ETAR and decreased ETBR expression. Comparing with control, there was higher ET-1 in umbilical plasma, maternal plasma, and placental vessels from preeclampsia. <em>In utero</em> hypoxia increased plasma ET-1 in fetal lambs and ewes. Hypoxia promoted ET-1 production in HUVEC. Role and expression of CaV1.2 was decreased in preeclampsia placental vessels, while high-molecular-weight caldesmon (CALD1), the marker of contractile phenotype of smooth muscle cells, was significantly increased. ET-1 treatment increased CALD1 in placental explants and in HUVSMC via ETAR/ETBR.</div></div><div><h3>Conclusion</h3><div>The present study firstly demonstrated ET-1 induced greater contraction in preeclampsia placental chorionic plate veins via ETAR/ETBR, instead of via weaker CaV1.2. <em>In utero</em> hypoxia promoted plasma ET-1 in fetal lambs and ewe, similar to that in preeclampsia. ET-1, binding with ETAR/ETBR increased CALD1, which was associated with stronger contraction in preeclampsia. The data provided important information in preeclampsia onset.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 165-174"},"PeriodicalIF":3.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic effects of exercise on improving the adverse placental environment associated with maternal overweight and obesity","authors":"Chen Wang,&nbsp;Minghui Liu,&nbsp;Jie Yan,&nbsp;Jie Ning,&nbsp;Shuxian Wang,&nbsp;Huixia Yang","doi":"10.1016/j.placenta.2024.10.018","DOIUrl":"10.1016/j.placenta.2024.10.018","url":null,"abstract":"<div><h3>Objective</h3><div>We aimed to explore whether maternal exercise has epigenetic effects on improving the adverse placental environment associated with maternal overweight/obesity.</div></div><div><h3>Methods</h3><div>Based on samples collected from an RCT cohort, differentially methylated genes and expressed protein-coding RNA were identified in the placentas of 16 women with overweight and obesity who did or did not participate in an exercise intervention using the Infinium HumanMethylation850 BeadChip array and RNA Sequencing-Based lncRNA Profiling. Potential target genes were further identified by integrating this information. Then, the target genes' methylation and expression levels were verified, and correlation analysis was performed with placental oxidative stress markers and participants’ clinical metabolic parameters.</div></div><div><h3>Results</h3><div>A total of 3608 significant differentially methylated probes were detected. The CBR1 gene, which was previously identified as a possible antioxidant, was significantly hypomethylated at CPG site promoter regions in placentas from the exercise group, and CBR1 gene expression levels were significantly higher. CBR1 gene expression levels were negatively associated with DNA methylation levels. Furthermore, CBR1 gene expression levels were negatively correlated with MDA levels in both placenta and cord blood samples and insulin resistance levels in late pregnancy. Additionally, CBR1 methylation levels were positively correlated with fasting plasma glucose and insulin resistance levels in late pregnancy.</div></div><div><h3>Conclusion</h3><div>DNA methylation is involved in the ameliorating effect of exercise on the adverse placental environment associated with maternal overweight/obesity.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 329-337"},"PeriodicalIF":3.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}