Placenta最新文献

{"title":"Lactate promotes premature aging of preeclampsia placentas through histone lactylation-regulated GADD45A","authors":"Xiang Li ,&nbsp;Qianghua Wang ,&nbsp;Jiaojiao Fei ,&nbsp;Zhixin Jin ,&nbsp;Yue Wu ,&nbsp;Yafen Tao ,&nbsp;Chuanyue Jiang ,&nbsp;Xuegu Wang ,&nbsp;Nana Yang ,&nbsp;Biao Ding ,&nbsp;Chengli Dou","doi":"10.1016/j.placenta.2025.01.010","DOIUrl":"10.1016/j.placenta.2025.01.010","url":null,"abstract":"<div><h3>Background</h3><div>Premature placental aging has been linked to preeclampsia (PE), with lactate identified as a promoter of cellular senescence in various cell types. In this study, we explored the role and underlying mechanisms of lactate in driving premature placental aging associated with PE.</div></div><div><h3>Methods</h3><div>To evaluate senescence markers in placental samples or trophoblast cells, we conducted SA-β-Gal staining, western blotting, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and immunofluorescence assays. SiRNA transfection was used to reduce GADD45A expression in HTR-8/SVneo cells exposed to lactate. Additionally, chromatin immunoprecipitation-qPCR (ChIP-qPCR) was used to analyze histone lactylation at the GADD45A promoter region.</div></div><div><h3>Results</h3><div>SA-β-Gal staining indicated a significant increase in senescent cell proportions in placentas from PE patients compared to controls. Treatment with lactate enhanced senescence in trophoblast cells, leading to an increase in P16 expression. RNA sequencing analysis showed that genes differentially expressed in lactate-treated cells were involved in pathways linked to cellular senescence. Additionally, lactate augmented GADD45A expression and increased histone lactylation at its promoter region, while knocking down GADD45A in trophoblast cells mitigated the senescence induced by lactate.</div></div><div><h3>Conclusions</h3><div>Lactate promotes trophoblast senescence through epigenetic upregulation of GADD45A expression, offering fresh perspectives on the molecular mechanisms and potential treatment targets for PE.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"161 ","pages":"Pages 39-51"},"PeriodicalIF":3.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to Group B Streptococcus-induced chorioamnionitis alters the proteome of placental extracellular vesicles.","authors":"Seline Vancolen, Mathilde Chevin, Bernard Robaire, Guillaume Sébire","doi":"10.1016/j.placenta.2025.01.008","DOIUrl":"https://doi.org/10.1016/j.placenta.2025.01.008","url":null,"abstract":"<p><strong>Introduction: </strong>Group B Streptococcus (GBS) is an opportunistic pathogen that can induce chorioamnionitis (CA), increasing the risk of neurodevelopmental disorders (NDDs) in the offspring. The placenta facilitates maternal-fetal communication through the release of extracellular vesicles (EVs), which may carry inflammatory molecules such as interleukin (IL)-1. Although the role of EVs in immune modulation is well established, their specific characterization in the context of GBS-induced CA has not yet been investigated. Understanding placental-derived EVs could further define how IL-1 and other inflammatory factors contribute to NDDs.</p><p><strong>Methods: </strong>We used an established rat model of GBS-induced CA. EVs from control and GBS infected dams were isolated from placentas and characterized using nanoparticle tracking analysis and transmission electron microscopy. The protein content was assessed via mass spectrometry, followed by subsequent pathway analysis. ELISA was used to quantify cytokine levels.</p><p><strong>Results: </strong>GBS-infected placentas exhibited calcification and increased weight, while fetal weight decreased. Analysis of the proteome from control versus GBS placental EVs revealed distinct profiles, with many proteins involved in the innate immune response, including alarmins (S100A8/9), complement pathways, and cytokine signaling pathways. Pathway analysis highlighted IL-1α and IL-1β identified as key upstream regulators. Notably, EVs from GBS-infected males showed a 44-fold increase in intracellular IL-1β compared to controls.</p><p><strong>Discussion: </strong>These findings indicate that GBS-induced CA alters the protein content of EVs from placental cells. Our findings of increased IL-1β-associated EVs highlight the need for further investigation into the role of these cytokines from GBS-exposed placentas and their role in brain injuries leading to NDDs.</p>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of senescence-associated transcripts in the human placenta","authors":"Oren Barak ,&nbsp;Alexander D. Bauer ,&nbsp;W. Tony Parks ,&nbsp;Tyler C. Lovelace ,&nbsp;Panayiotis V. Benos ,&nbsp;Tianjiao Chu ,&nbsp;Yoel Sadovsky","doi":"10.1016/j.placenta.2025.01.009","DOIUrl":"10.1016/j.placenta.2025.01.009","url":null,"abstract":"<div><h3>Introduction</h3><div>Fusion of mononucleated cytotrophoblasts into syncytium leads to trophoblast senescence. Yet, premature senescence is associated with preeclampsia, fetal growth restriction (FGR), and related obstetrical syndromes. A set of 28 transcripts that comprise senescence-associated secretory phenotype (SASP) was recently described in placentas from women with preeclampsia. We posited that this transcript set is uniquely regulated in late-term placentas or in placentas derived from participants with major obstetrical syndromes.</div></div><div><h3>Methods</h3><div>Using our large placental RNAseq bank, we analyzed data from healthy participants (n = 33) with histologically normal placentas, representing delivery at 37–41 weeks. To represent diseases, we included RNAseq data from participants (n = 220) with severe preeclampsia, FGR, FGR with a hypertensive disorder (FGR + HDP), or spontaneous preterm delivery, and healthy controls (n = 129). We also assessed the expression of several SASPs in primary human trophoblasts that were exposed <em>in vitro</em> to hypoxia, reduced differentiation, or ferroptotic or apoptotic signals.</div></div><div><h3>Results</h3><div>Among the 28 SASP transcripts analyzed, eight had a significant change between deliveries at &lt;37 weeks <em>vs</em> ≥ 41 weeks, including upregulation of <em>FSTL3</em>, <em>IL1RL1</em>, <em>INHBA</em>, and <em>VEGFA</em> and downregulation of <em>STC1</em>, <em>RARRES2</em>, <em>MRC2</em>, and <em>SELP</em>. The expression of SASP mRNAs was enriched in the placentas from the assessed syndromes, with most expression changes in placentas from FGR/HDP. Our <em>in vitro</em> analysis associated hypoxia or apoptosis with altered expression of <em>FSTL3</em>, <em>VEGFA</em>, and <em>DKK1</em>.</div></div><div><h3>Discussion</h3><div>A set of placental SASPs defines late-term placentas, placental dysfunction-related clinical syndromes, and <em>in vitro-</em>defined trophoblast injury. Trophoblastic SASP signatures may assist in characterizing placental senescence in health and disease.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"161 ","pages":"Pages 31-38"},"PeriodicalIF":3.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression of chromosome 19 miRNA cluster members during insulin sensitivity changes in pregnancy","authors":"Fernanda Alvarado-Flores ,&nbsp;Tianjiao Chu ,&nbsp;Patrick Catalano ,&nbsp;Yoel Sadovsky ,&nbsp;Perrie O'Tierney-Ginn","doi":"10.1016/j.placenta.2025.01.007","DOIUrl":"10.1016/j.placenta.2025.01.007","url":null,"abstract":"<div><h3>Hypothesis</h3><div>Declines in insulin sensitivity during pregnancy important for fetal growth are associated with impairments in skeletal muscle post-receptor insulin signaling. The primary initiator of these changes is unknown but believed to originate in the placenta. We hypothesize that placental miRNAs are associated with maternal sensitivity changes and impact insulin-sensitive mechanisms in target tissues <em>in vitro</em>.</div></div><div><h3>Methods</h3><div>Using qPCR, miRNA expression was measured in plasma in early (12–16 wk) and late (34–36 wk) gestation (N = 39) and placental tissue at term (37–41 weeks) (N = 142) collected from independent cohorts. Insulin-sensitive glucose uptake was measured in human skeletal muscle myoblasts exposed to miRNA mimics <em>in vitro</em>. Multi-linear and binomial regression models were generated to test for associations between miRNAs, insulin sensitivity and fetal growth outcomes, adjusting for relevant clinical variables. P &lt; 0.05 was considered significant.</div></div><div><h3>Results</h3><div>Placental expression of chromosome 19 miRNA cluster (C19MC) members was higher in patients with obesity and positively correlated with maternal HOMA-IR (Homeostatic Model Assessment for Insulin Resistance; miR-516b-5p, miR-517a-3p, miR-1283). Placental expression of miR-517a-3p was higher in offspring with high adiposity and birthweight. Plasma miR-517a-3p in early and late pregnancy was related to decreases in insulin sensitivity during pregnancy. Mimics for miR-517a-3p and miR-524–3p both impaired insulin-sensitive glucose uptake in human skeletal myocytes <em>in vitro</em>.</div></div><div><h3>Discussion</h3><div>Our findings based on data from two independent pregnancy cohorts and <em>in vitro</em> studies support a role for members of the C19 cluster of miRNAs – particularly miR-517a-3p - in physiological changes in insulin sensitivity over pregnancy, which may impact fetal growth.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"161 ","pages":"Pages 23-30"},"PeriodicalIF":3.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143029305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Congenital Chagas disease: The importance of Trypanosoma cruzi-placenta interactions.","authors":"Castillo Christian, Liempi Ana, Fernández-Moya Alejandro, Guerrero-Muñoz Jesús, Araneda Sebastian, Cáceres Gabriela, Alfaro Sebastián, Gallardo Christian, Maya Juan Diego, Müller Marioly, Kemmerling Ulrike","doi":"10.1016/j.placenta.2025.01.004","DOIUrl":"https://doi.org/10.1016/j.placenta.2025.01.004","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myeloperoxidase-mediated immature dendritic cell promotes vascular remodeling and functional placenta formation","authors":"Feng Gao ,&nbsp;Jiabin Jiang ,&nbsp;Yunfeng Lin ,&nbsp;Juan Tang ,&nbsp;Yanqi Ke ,&nbsp;Yuqing Zheng ,&nbsp;Qingquan Chen ,&nbsp;Qicai Liu","doi":"10.1016/j.placenta.2025.01.006","DOIUrl":"10.1016/j.placenta.2025.01.006","url":null,"abstract":"<div><h3>Introduction</h3><div>The distribution of myeloperoxidase (MPO) and dendritic cells (DCs) in sponge trophoblast cells may contribute to the syncytialisation of trophoblast cells and the establishment of uterine placental circulation. Our previous series of studies have shown that MPO plays an important role in angiogenesis and repair, and placental vascular dysfunction can lead to serious pregnancy complications and even miscarriage.</div></div><div><h3>Methods</h3><div>Mouse model of MPO knockout was constructed, and the crosstalk between MPO and dendritic cells (DC) cells was investigated to determine whether MPO is involved in the pregnancy process. Abnormal decidual vasculogenesis in <em>MPO</em>^{<em>−/−</em>} pregnant mice was also suggested by RNA-seq analysis of uterine tissues from pregnant mice. In addition, we extracted mouse BMDC, analyzed the relationship between Mpo and BMDC, and established a co-culture system between BMCD and endothelial cells.</div></div><div><h3>Results</h3><div>It was found that angiogenesis in the decidual tissue of <em>MPO</em>^{<em>−/−</em>} mice was impaired in early pregnancy, while in WT mice of the same pregnancy period, MPO and DC were observed to co-localize at the site of vascular development, it was found that immature BMDC can significantly promote the tube formation ability of endothelial cells in vitro, while MPO it is the key for BMDC to maintain immature phenotype.</div></div><div><h3>Discussion</h3><div>In conclusion, our study reveals a new role of immature DCs induced by MPO in promoting vascular remodeling of decidual tissue and functional placental formation.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"161 ","pages":"Pages 1-13"},"PeriodicalIF":3.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association studies of vasoactive genes and preeclampsia in taiwan","authors":"Mei-Lin Yang ,&nbsp;Fong-Ming Chang ,&nbsp;Meng-Hsing Wu ,&nbsp;Chung-Hwan Chen ,&nbsp;Tsung-Lin Cheng ,&nbsp;Lin Kang","doi":"10.1016/j.placenta.2025.01.005","DOIUrl":"10.1016/j.placenta.2025.01.005","url":null,"abstract":"<div><h3>Background</h3><div>Preeclampsia (PE) is a serious condition characterized by hypertension and proteinuria after 20 weeks of gestation. The exact cause of PE is unknown but may involve abnormalities in the renin-angiotensin-aldosterone system (RAAS) and endothelial nitric oxide synthase (eNOS). Genetic variations in angiotensinogen (<em>AGT</em>), angiotensin-converting enzyme (<em>ACE</em>), and <em>eNOS</em> genes have been associated with PE. This study aimed to investigate the potential of vasoactive-related gene polymorphisms as indicators of susceptibility to preeclampsia in Taiwanese women.</div></div><div><h3>Methods</h3><div>A total of 109 women with severe PE and 150 controls from the Taiwanese population were genotyped for specific vasoactive gene polymorphisms, including M235T and T174M polymorphisms of <em>AGT</em> gene, insertion/deletion (I/D) polymorphism in <em>ACE</em> gene, and G894T (Glu298Asp) polymorphism and 27bp variable number of tandem repeats (VNTR 3/4/5) polymorphism of the <em>eNOS</em> gene. The association between genotype and disease was assessed using Chi-square tests.</div></div><div><h3>Results</h3><div>The study found no significant differences in the M235T and T174M polymorphisms of <em>AGT</em> gene between the PE and control groups. However, haplotype frequencies for the M235T and T174M polymorphisms exhibited a significant association with PE. The genotype distributions of the I/D polymorphism of <em>ACE</em> gene showed a significant difference between PE and control groups. Additionally, no significant differences were detected in the polymorphisms of the <em>eNOS</em> gene between PE and control groups.</div></div><div><h3>Conclusion</h3><div>The findings of this study suggest that the <em>AGT</em> M235T-T174M haplotype and <em>ACE</em> insertion/deletion polymorphism may contribute to the development of preeclampsia and could serve as susceptibility markers for preeclampsia in Taiwanese women.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"161 ","pages":"Pages 14-22"},"PeriodicalIF":3.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered placental phenotype and increased risk of placental pathology in fetal spina bifida: A matched case-control study","authors":"Marina White ,&nbsp;David Grynspan ,&nbsp;Jayden Arif-Pardy ,&nbsp;Tim Van Mieghem ,&nbsp;Kristin L. Connor","doi":"10.1016/j.placenta.2024.12.004","DOIUrl":"10.1016/j.placenta.2024.12.004","url":null,"abstract":"<div><h3>Introduction</h3><div>Spina bifida (SB) remains one of the most common congenital anomalies and associates with significant comorbidities in the fetus, which may, in part, be driven by placental maldevelopment. We hypothesised that placental pathologies would be more prevalent in fetuses with SB compared to fetuses without congenital anomalies.</div></div><div><h3>Methods</h3><div>Placental pathology and transcriptome were evaluated for fetuses with isolated open SB born preterm (cases; n = 12) and control fetuses without congenital anomalies (n = 22) born at full term (FT) or preterm (PT). We evaluated associations between study group and placental histopathology, and between placental histopathology and gene expression.</div></div><div><h3>Results</h3><div>Placental weight was lower in cases than PT controls (median [IQR]: 263 g [175, 370] vs. 455 g [378, 560], p = 0.001). Placental villi structural phenotype was different in cases, where proportion of immature intermediate villi was higher in cases than PT controls (32.5 % [6.3, 56.3] vs. 10 % [5, 13.8], p = 0.01), but cases and FT controls had similar proportions of mature intermediate (10 % [5, 10] vs. 10 % [8.75, 11.25]) and terminal villi (22.5 % [11.3, 43.8] vs. 30 % [20, 36.3]), and similar odds of having many syncytial knots (adjusted odds ratio [aOR] = 6 [0.2, 369]). Case placentae also had higher odds of having many Hofbauer cells (aOR = 16.2 [1.4, 580], p = 0.02) and a thick syncytial membrane (aOR = 146 [3, 3.46e5], p = 0.007). Gene expression in immune/inflammatory processes, spinal cord injury, and Hedgehog and Wnt signaling pathways were associated with placental maturity in cases.</div></div><div><h3>Discussion</h3><div>Improved knowledge on placental phenotypes in SB increases our understanding of mechanisms that may drive comorbidities, and may ultimately inform efforts to reduce offspring morbidity and mortality.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"159 ","pages":"Pages 107-118"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene expression profiles based on maternal plasma cfDNA nucleosome footprints indicate fetal development and maternal immunity changes during pregnancy progress","authors":"Min Zhang ,&nbsp;Kun Li ,&nbsp;Xiang Huang ,&nbsp;Huiling Zhou ,&nbsp;Jiayu Tan ,&nbsp;Zhiwei Guo ,&nbsp;Xingyu Wei ,&nbsp;Yuming Liu ,&nbsp;Shi Weng ,&nbsp;Guojun Ouyang ,&nbsp;Xuexi Yang ,&nbsp;Wenbo Hao ,&nbsp;Fenxia Li","doi":"10.1016/j.placenta.2024.12.005","DOIUrl":"10.1016/j.placenta.2024.12.005","url":null,"abstract":"<div><h3>Background</h3><div>Pregnancy significantly alters the maternal immune system, affecting fetal development. The collection of tissues from the human placenta and fetus is not ethically or practically feasible at various gestational stages, thus limiting the study of gene expression in the fetus and placenta. Recent studies have shown that plasma cell-free DNA (cfDNA) nucleosome patterns can predict gene expression in the source tissue, offering insights into an individual's health status. This study aimed to identify pregnancy-related gene expression changes across gestational periods using cfDNA nucleosome distribution to understand fetal development and maternal immune changes.</div></div><div><h3>Methods</h3><div>Plasma samples were collected from 150 healthy pregnant women in different trimesters (early, mid, and late) and 32 healthy nonpregnant women. The correlation between gene expression and physiological changes during pregnancy was evaluated by inferring differential expression profiles around the transcription start site (TSS) using cfDNA nucleosome distribution patterns obtained through whole-genome sequencing. We utilized Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses to annotate differentially expressed genes with the mother and fetus.</div></div><div><h3>Results</h3><div>We identified gene expression changes that support the regulation of fetal development and immune system function during pregnancy. Differential coverage genes were mainly enriched in pathways related to transcription and translation, organic compound metabolism, and immune regulation. In addition, differentially expressed genes with significant temporal trends were identified. Among them, the upregulated differential genes were mainly related to development, whereas those with downregulated trends were mainly related to the immune system response. This indicates that differential changes of the placenta and maternal are significantly correlated with the pregnancy status.</div></div><div><h3>Discussion</h3><div>This study demonstrated the differential gene expression represented by the characteristic distribution of cfDNA nucleosome in maternal peripheral blood can effectively capture significant changes in maternal immunity and fetal development throughout pregnancy stages. It may help identify abnormal gene expression patterns associated with complications in pregnancy and childbirth, enhancing the quality of life and safety for both mother and fetus.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"159 ","pages":"Pages 84-92"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profilin-1 levels in preeclampsia: Associations with disease and adverse neonatal outcomes","authors":"Sadullah Özkan ,&nbsp;Alperen Aksan ,&nbsp;Fahri B. Fıratlıgil ,&nbsp;Dilara Kurt ,&nbsp;Serap Sucu ,&nbsp;Aslıhan Coşkun ,&nbsp;Kadriye Yakut Yücel ,&nbsp;A. Turhan Çağlar ,&nbsp;Yaprak Engin Üstün","doi":"10.1016/j.placenta.2024.12.012","DOIUrl":"10.1016/j.placenta.2024.12.012","url":null,"abstract":"<div><h3>Background</h3><div>Preeclampsia is a serious pregnancy complication requiring early detection to improve outcomes. Profilin-1 (PFN1), linked to vascular dysfunction, may serve as a biomarker for diagnosing preeclampsia and predicting adverse neonatal outcomes. The aim of this study was to determine the serum Profilin-1 levels in patients diagnosed with preeclampsia and to investigate its association with disease severity and adverse neonatal outcomes.</div></div><div><h3>Methods</h3><div>A prospective cross-sectional study was conducted at Etlik City Hospital involving 40 women with preeclampsia and 40 healthy controls. Serum PFN1 levels were measured by ELISA and results were compared between groups. The results were compared between the groups. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic performance of PFN1.</div></div><div><h3>Results</h3><div>Serum PFN1 levels were significantly higher in the preeclampsia group compared to controls (46.48 [30.23–60.29] vs. 26.41 [19.65–41.76], p &lt; 0.001). The ROC curve showed good diagnostic accuracy for PFN1 in detecting preeclampsia with an AUC of 0.741 (95 % CI: 0.631–0.832, p &lt; 0.001), a sensitivity of 95 % and a specificity of 42.5 %. PFN1 levels were also associated with composite neonatal outcomes, with an AUC of 0.622 (95 % CI: 0.520–0.716, p = 0.042).</div></div><div><h3>Discussion</h3><div>PFN1 is a potential biomarker for the diagnosis of preeclampsia. However, further studies are needed to validate its role in predicting adverse neonatal outcomes and to improve its specificity for clinical use.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"159 ","pages":"Pages 140-145"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}