Placenta最新文献

{"title":"Development and validation of the placenta-QUS model for the detection of placenta-mediated diseases using quantitative ultrasound measurements: An Ex Vivo proof-of-concept study","authors":"Farah Deeba ,&nbsp;Ricky Hu ,&nbsp;Victoria Lessoway ,&nbsp;Jefferson Terry ,&nbsp;Denise Pugash ,&nbsp;Chantal Mayer ,&nbsp;Jennifer Hutcheon ,&nbsp;Robert Rohling","doi":"10.1016/j.placenta.2024.11.004","DOIUrl":"10.1016/j.placenta.2024.11.004","url":null,"abstract":"<div><h3>Introduction</h3><div>Placenta-mediated diseases are associated with structural changes in the placenta. Quantitative Ultrasound (QUS) imaging measures the acoustic properties of the tissue, which are correlated to the underlying tissue structure. We aimed to develop and validate a diagnostic prediction model using QUS measurements for pre-eclampsia (PE) and small-for-gestational-age (SGA) fetuses/neonates.</div></div><div><h3>Methods</h3><div>For this prospective case-control study, placentas were collected from a group of women who delivered via cesarean section at BC Women's Hospital, Vancouver, Canada. Ultrasound data were collected and processed to compute three QUS parameters, namely, attenuation coefficient estimate (ACE), integrated backscatter coefficient (IBC), and effective scatterer diameter (ESD) from the placentas. We developed a logistic regression model using QUS parameters as predictors. The primary outcome was the occurrence of SGA and PE.</div></div><div><h3>Results</h3><div>The dataset consisted of 47 placentas, of which 25 placentas were complicated by SGA/PE. The final placenta-QUS model included quadratic and interaction terms of ACE, IBC, and ESD parameters. The placenta-QUS model was well-calibrated, with a calibration slope of 0.99 (0.57–1.05) and a calibration intercept of 0.003 (−0.02 − 0.22). The model predicted the SGA/PE complicated pregnancies with an apparent Area Under the Receive Operating Characteristic Curve (AUROC) of 0.89 (95 % CI: 0.78–0.98). The optimism-adjusted AUROC was 0.88 (95 % CI: 0.78–0.98).</div></div><div><h3>Discussion</h3><div>A model for SGA and PE has been developed using QUS measures from the placenta <em>ex vivo</em>. The model showed promising performance in detecting SGA/PE. Future studies will be performed to assess the model performance using QUS measures <em>in utero</em>.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 293-300"},"PeriodicalIF":3.0,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspartame intake during pregnancy induces placental dysfunction through impaired mitochondrial function and biogenesis modulation","authors":"Yang-Ching Chen ,&nbsp;Yen-Chia Yeh ,&nbsp;Yu-Fang Lin ,&nbsp;Shih-Yuan Hsu ,&nbsp;Jacus S. Nacis ,&nbsp;Jhih-Wei Hsu ,&nbsp;Rong-Hong Hsieh","doi":"10.1016/j.placenta.2024.11.003","DOIUrl":"10.1016/j.placenta.2024.11.003","url":null,"abstract":"<div><h3>Introduction</h3><div>Aspartame is a nonnutritive sweetener (NSS), which is widely used in foods and beverages worldwide. The safety of aspartame, a commonly used artificial sweetener, has been debated. Here, we investigated the potential effects and underlying mechanisms of aspartame consumption during pregnancy on placental dysfunction and birth outcomes.</div></div><div><h3>Methods</h3><div>Female Sprague Dawley rats were exposed to a low (30 mg/kg) or high (60 mg/kg) dose of aspartame before and during pregnancy; moreover, we assessed placental histopathological structure, oxidative stress markers, and mitochondrial function. In addition, we explored how aspartame affects birth weight in a human maternal–infant cohort.</div></div><div><h3>Results</h3><div>In animal study revealed that aspartame treatment of female rats for 14 weeks (12 week before pregnancy and 18 days of gestation) causes a significant reduction in the number and weight of fetuses, as well as damage to placental structure. These effects are linked to increased oxidative stress in the placenta, possibly damaging placental trophoblasts, impairing mitochondrial function, and initiating a compensatory mitochondrial biosynthesis mechanism. In the human pregnant cohort revealed that aspartame reduces birth weight considerably.</div></div><div><h3>Discussion</h3><div>These findings suggested the potential risks associated with aspartame consumption during pregnancy. Therefore, the safety of aspartame, particularly in pregnant individuals, should be reconsidered; specifically, tailored, acceptable daily intake guidelines should be developed for aspartame in different populations.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 285-292"},"PeriodicalIF":3.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trophoblast proliferation is higher in female than in male preeclamptic placentas","authors":"N. Barapatre ,&nbsp;L. Hansen ,&nbsp;C. Kampfer ,&nbsp;T. Rübelmann ,&nbsp;C. Schmitz ,&nbsp;F. von Koch ,&nbsp;H.G. Frank","doi":"10.1016/j.placenta.2024.10.016","DOIUrl":"10.1016/j.placenta.2024.10.016","url":null,"abstract":"<div><h3>Introduction</h3><div>Preeclampsia (PE) is a pregnancy-specific hypertensive disorder with inflammatory complications. There are no known placental histopathological features, which are unique to PE. It is often pooled with the fetal growth restriction (FGR) under a single umbrella pathophysiology, the maternal vascular malperfusion (MVM). The aim of this study is to assess the villous trophoblast and the villous tree quantitatively in PE placentas and to identify morpholgical correlates unique to PE.</div></div><div><h3>Methods</h3><div>20 PE placentas (10 female and 10 male) and 20 Control placentas (10 female and 10 male) were included in the study. The villous trophoblast and the villous tree were assessed quantitatively by Stereology and 3D Microscopy. For Stereology measurements, the villous tree was classified in contractile and non-contractile parts based on immunohistochemical detection of perivascular myofibroblasts.</div></div><div><h3>Results</h3><div>The density of proliferative trophoblast nuclei is increased, whereas the density of non-proliferative trophoblast nuclei is decreased in female PE placentas. The male PE placentas do not show this effect. Though no significant difference in the diffusion distance was observed, the non-contractile villi and the fetal vessels inside show a significantly reduced volume in PE placentas. The branching index of the villous tree is lower in PE placentas in general. However, in female PE placentas the deviation is accentuated.</div></div><div><h3>Conclusion</h3><div>In PE, the villous trophoblast shows a sexually dimorphic alteration in the density of proliferative and non-proliferative nuclei, which is inherently different from FGR.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 310-317"},"PeriodicalIF":3.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylation aberrations in partner spermatozoa and impaired expression of imprinted genes in the placentae of early-onset preeclampsia","authors":"Sweta Nair ,&nbsp;Kushaan Khambata ,&nbsp;Himangi Warke ,&nbsp;Vandana Bansal ,&nbsp;Anushree Patil ,&nbsp;Zakiya Ansari ,&nbsp;Nafisa H. Balasinor","doi":"10.1016/j.placenta.2024.10.068","DOIUrl":"10.1016/j.placenta.2024.10.068","url":null,"abstract":"<div><h3>Introduction</h3><div>Disturbed paternal epigenetic status of imprinted genes has been observed in infertility and recurrent spontaneous abortions. Shallow placentation has been associated with early-onset preeclampsia. Hence, the present study aimed to investigate the methylation patterns of imprinted genes involved in placental development, in the spermatozoa of partners of women experiencing preeclampsia.</div></div><div><h3>Methods</h3><div>The study involved recruitment of couples into preeclampsia (n = 14) and control (n = 25) groups. Methylation analysis of imprinted gene differentially methylated regions (DMRs) and <em>LINE1</em> repetitive element was carried out by pyrosequencing in the spermatozoa and placental villi. Global 5 mC levels in the spermatozoa were measured through ELISA. Expression of imprinted genes was quantified in the placental villi by real time qPCR. Association of birth weight with DNA methylation and gene expression was assessed.</div></div><div><h3>Results</h3><div>KvDMR, <em>PEG3</em> DMR, <em>PEG10</em> DMR and <em>DLK1</em>-<em>GTL2</em> IG-DMR were differentially methylated in the spermatozoa and placental villi of preeclampsia group. Global 5 mC content and <em>LINE1</em> methylation levels did not differ between the spermatozoa of the two groups. Increased transcript levels of <em>PEG3</em>, <em>IGF2</em>, <em>DLK1, PHLDA2</em> and <em>CDKN1C</em> were observed in the preeclamptic placental villi. Birth weight showed significant association with KvDMR, <em>PEG10</em> DMR, <em>DLK1-GTL2</em> IG-DMR and <em>LINE1</em> methylation levels in the spermatozoa. <em>DLK1</em> expression levels showed a negative association with birth weight.</div></div><div><h3>Discussion</h3><div>The study highlighted the paternal contribution to early-onset preeclampsia, in the form of disrupted sperm DNA methylation patterns at imprinted gene loci. These loci, after further evaluation in future studies, could serve as sperm-based preeclampsia predictive markers, for couples planning pregnancy.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 275-284"},"PeriodicalIF":3.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BMP5 promotes trophoblast functions upon N-glycosylation via the BMP5-SMAD1/5 signaling pathway in preeclampsia","authors":"Hao Wang ,&nbsp;Ningning Fan ,&nbsp;Xinyuan Cui ,&nbsp;Ru Xie ,&nbsp;Ying Tang ,&nbsp;Aline M. Thomas ,&nbsp;Shen Li ,&nbsp;Jian V. Zhang ,&nbsp;Shuai Liu ,&nbsp;Huamin Qin","doi":"10.1016/j.placenta.2024.11.002","DOIUrl":"10.1016/j.placenta.2024.11.002","url":null,"abstract":"<div><h3>Introduction</h3><div>Preeclampsia (PE) is one of the most common pregnancy-related complications worldwide and currently lacks an effective treatment. While trophoblast cell dysfunction has been identified as the fundamental cause of PE, the underlying mechanisms remain unclear. Bone morphogenetic protein 5 (BMP5) is a secreted glycoprotein highly expressed in the placenta that is involved in cell proliferation, migration, and invasion. However, the role and mechanism of BMP5 glycosylation of trophoblast cell function remain unclear.</div></div><div><h3>Methods</h3><div>The expression of BMP5 and N-glycosylation in preeclamptic placental tissues was investigated. We predicted and validated the N-glycosylation sites of BMP5. Additionally, we evaluated the effect of BMP5 N-glycosylation on the proliferation, migration, invasion, and angiogenesis of human immortalized trophoblastic HTR-8/SVneo cells. Furthermore, the role of N-glycosylated BMP5 in activating the BMP5-SMAD1/5 signaling pathway and regulating trophoblastic cell functions was explored.</div></div><div><h3>Results</h3><div>Our study reveals that PHA-E + L (recognizing branching N-glycans) reactive N-glycans and BMP5 expression levels are lower in preeclamptic villous tissues compared to normal placental tissues. Additionally, we demonstrated that BMP5 is an N-glycosylation-modified protein. Furthermore, N-glycosylated BMP5 promoted the functional trophoblastic cells (HTR-8/SVneo). We also revealed that N-glycosylation of BMP5 regulates multiple cell functions through the BMP5-SMAD1/5 signaling pathway.</div></div><div><h3>Conclusion</h3><div>N-glycosylated BMP5 promotes trophoblast cell proliferation, migration, invasion, and angiogenesis. This study provides mechanistic insight as to how N-glycosylation of BMP5 in trophoblast cells can contribute to the pathogenesis of preeclampsia and provides a new basis for its diagnosis and treatment.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 240-252"},"PeriodicalIF":3.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Unveiling placental development in circadian rhythm-disrupted mice: A photo-acoustic imaging study on unstained tissue” [Placenta 158 (2024) 57-61]","authors":"M.N. Cizmeciyan ,&nbsp;N.I. Bektas ,&nbsp;N. Derin ,&nbsp;T. Denizaltı ,&nbsp;A. Khoshzaban ,&nbsp;M.B. Unlu ,&nbsp;C. Celik-Ozenci","doi":"10.1016/j.placenta.2024.10.066","DOIUrl":"10.1016/j.placenta.2024.10.066","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Page 216"},"PeriodicalIF":3.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental extracellular vesicles promoted cervical tumour tissue undergoing death","authors":"Lin Wang ,&nbsp;Jinqiu Zhang ,&nbsp;Angang Sun ,&nbsp;Yongxiang Yin ,&nbsp;Ye Shen ,&nbsp;Dengxin Zhang ,&nbsp;Qi Chen ,&nbsp;Min Zhao","doi":"10.1016/j.placenta.2024.10.069","DOIUrl":"10.1016/j.placenta.2024.10.069","url":null,"abstract":"<div><h3>Background</h3><div>Cervical cancer is a leading cause of death in developing countries. Although the placenta is a tumor-like organ, the placental development, including invasive function, is well controlled. One mechanism is that extracellular vesicles (EVs) released from the placenta contribute to this regulation. Placental EVs carry functional proteins and regulatory RNAs. Our previous study reported that placental EVs inhibited ovarian cancer growth <em>in vitro</em> and <em>in vivo</em>.</div></div><div><h3>Methods</h3><div>Whether the inhibitory effect induced by placental EVs also applies to cervical cancer, a non-endocrine-related cancer, in this study, we first co-cultured the cervical tumour tissues with placental explants.</div></div><div><h3>Results</h3><div>Co-culturing cervical tumour tissues (n = 7) with placental explants showed necrotic signs and increased levels of senescence-associated proteins and death-associated miRNAs, including miRNA-143-3p, miRNA-519a-5p and miRNA-199a-3p in tumour tissues. Additionally, treatment of HeLa cells with placental EVs reduced the viability of HeLa cells and inhibited the ability of invasion and migration of HeLa cells. Increased levels of senescence-associated proteins and reduced levels of proliferative proteins may contribute to the inhibitory effects in HeLa cells.</div></div><div><h3>Discussion</h3><div>placental EVs are involved in regulating placental development, and the delivery of cargo significantly impacts the functions of target cells. This study found that factors released from placental explants, likely placental EVs, had anti-tumour effects on the cervical tumour by inhibiting cervical cancer cell viability, invasion, and migration. Cargo in placental EVs, such as cellular death-associated miRNAs, may contribute to the inhibitory effects on cervical tumour.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 223-230"},"PeriodicalIF":3.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mid-late gestation leptin infusion induces placental mitochondrial and endoplasmic reticulum unfolded protein responses in a mouse model of preeclampsia","authors":"Jessica L. Faulkner ,&nbsp;Mayumi Takano ,&nbsp;Safia Ogbi ,&nbsp;Wen Tong ,&nbsp;Masahiko Nakata ,&nbsp;Desmond Moronge ,&nbsp;Tereza Cindrova-Davies ,&nbsp;Dino A. Giussani","doi":"10.1016/j.placenta.2024.11.001","DOIUrl":"10.1016/j.placenta.2024.11.001","url":null,"abstract":"<div><h3>Introduction</h3><div>Preeclamptic patients, both lean and obese, present with elevated leptin levels which are associated with the development of maternal endothelial dysfunction and adverse fetal outcomes, such as growth restriction, leading to low birth weight. Recent studies in pregnant mice demonstrate that mid-late gestation leptin infusion induces clinical characteristics of preeclampsia, including elevated maternal blood pressure, maternal endothelial dysfunction and fetal growth restriction. However, whether leptin triggers placental stress responses that contribute to adverse fetal outcomes as in preeclampsia is unknown.</div></div><div><h3>Methods</h3><div>In the current study we measured the expression of proteins involved in the endoplasmic reticulum (UPR^er) and mitochondrial (UPR^mt) unfolded protein responses in placentas of wild-type sham normal pregnant and leptin-infused preeclamptic mice.</div></div><div><h3>Results</h3><div>The data show that mid-late gestation leptin infusion induced activation of indices of placental UPR^er and UPR^mt, while reducing placental repair mechanisms to UPR^mt in preeclamptic mice. Mid-late gestation infusion with leptin upregulated markers of placental oxidative stress, reduced the placental expression levels of mitochondrial electron transport chain complexes I and II and increased the expression of placental endothelin-1 (ET-1) in preeclamptic mice. The leptin-induced activation of several placental UPR^mt markers as well as ET-1 levels correlated with fetal growth restriction and impaired maternal endothelial function in preeclamptic mice.</div></div><div><h3>Discussion</h3><div>Collectively, these data indicate that elevated levels of leptin in mid-late pregnancy in mice promote placental stress responses, akin to those in pregnant women with preeclampsia.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 253-262"},"PeriodicalIF":3.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental methylation and pro-inflammatory protein levels in cord blood","authors":"Sanne D. van Otterdijk ,&nbsp;Alexandra M. Binder ,&nbsp;Karin B. Michels","doi":"10.1016/j.placenta.2024.10.067","DOIUrl":"10.1016/j.placenta.2024.10.067","url":null,"abstract":"<div><h3>Introduction</h3><div>The neonates’ inflammatory response may in part be shaped during development by the placental epigenome, but evidence is scarce. We investigated the association between placental DNA methylation and pro-inflammatory proteins in cord blood.</div></div><div><h3>Methods</h3><div>A total of 249 mother-child dyads from the Harvard Epigenetic Birth Cohort were included in this study. Genome-wide methylation in placental DNA was assessed using the Illumina Human Methylation 450 Bead Chip array and verified by pyrosequencing. Cord blood inflammation markers assessed were interleukin-6, interleukin-8 and tumor necrosis factor α, intercellular adhesion molecule 1, serum amyloid A, and C-reactive protein.</div></div><div><h3>Results</h3><div>We identified differential placental DNA methylation of three loci in the <em>HIVEP3</em> gene shore region that were associated with TNFα protein levels in cord blood. TNFα levels were associated with mode of delivery, gestational age and birth order. Three other loci located in the open sea region of the <em>BCL11B</em> gene were associated with SAA protein levels in cord blood. SAA levels were associated with birthweight, gestational age, and infant sex.</div></div><div><h3>Conclusions</h3><div>Our results suggest a potential role for <em>HIVEP3</em> and <em>BCL11B</em> placental DNA methylation in the acute immune response of the neonate. These immune markers were correlated with several mother and child characteristics.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 231-239"},"PeriodicalIF":3.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis identified novel biomarker in invasive placenta accreta spectrum","authors":"Xiaoming Shi ,&nbsp;Ling Jin ,&nbsp;Xinlu Meng ,&nbsp;Xiao Huo ,&nbsp;Yan Sun ,&nbsp;Lixiang Xue ,&nbsp;Yuan Wei ,&nbsp;Yuanyuan Wang ,&nbsp;Zhongnan Yin ,&nbsp;Yangyu Zhao ,&nbsp;Lian Chen","doi":"10.1016/j.placenta.2024.10.023","DOIUrl":"10.1016/j.placenta.2024.10.023","url":null,"abstract":"<div><h3>Introduction</h3><div>Placenta accreta spectrum (PAS) disorders pose a grave threat to maternal life due to severe hemorrhage and the heightened risk of peripartum hysterectomy. Consequently, there's a pressing need for circulating biomarkers in clinical settings. MicroRNAs (miRNAs), being stable in peripheral circulation, hold promise as potential biomarkers for PAS.</div></div><div><h3>Methods</h3><div>This study recruited singleton live pregnancies, including cases of invasive PAS, placenta previa (PP), and controls, across three phases. Initially, RNA-seq of peripheral blood identified 6 miRNAs in the screening phase. Subsequently, in the training and validation phases, miR-23a-5p, along with its target genes ASF1B and CHTF8, were validated using qRT-PCR. The diagnostic value of these markers for PAS and adverse outcomes was evaluated using Receiver Operating Characteristic (ROC) curves.</div></div><div><h3>Results</h3><div>The results showed miR-23a-5p was down-regulated in PAS, whereas ASF1B and CHTF8 were up-regulated. miR-23a-5p had modest diagnostic efficiency for PAS and adverse outcomes, as the AUC were 0.689 and 0.711 respectively. However, when miR-23a-5p combined with CHTF8, the AUC can improve greatly to 0.869 in PAS diagnosis and 0.856 in prediction of adverse outcomes.</div></div><div><h3>Discussion</h3><div>We propose the miR-23a-5p plays a role in PAS pathogenesis through regulating cell proliferation, migration, invasion, apoptosis by targeting various genes. This study confirmed its potential value of miR-23a-5p combined with target gene CHTF8 as novel biomarkers for PAS and adverse outcomes.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"158 ","pages":"Pages 301-309"},"PeriodicalIF":3.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}