Placenta最新文献

{"title":"Corrigendum to “Identification of divergent placental profiles in clinically distinct pregnancy complications revealed by the transcriptome” [Placenta 154 (2024): 184–192 / PMID: 39042974]","authors":"Camille Couture , Maxime Caron , Pascal St-Onge , Marie-Eve Brien , Daniel Sinnett , Dorothée Dal Soglio , Sylvie Girard","doi":"10.1016/j.placenta.2025.05.012","DOIUrl":"10.1016/j.placenta.2025.05.012","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"168 ","pages":"Page 124"},"PeriodicalIF":3.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SPARC expression in the mouse decidua, placenta, and fetus: correlations with SPP1 expression","authors":"Joe W. Cain ,&nbsp;David W. Erikson ,&nbsp;Heewon Seo ,&nbsp;Alexandria Ross ,&nbsp;Robert C. Burghardt ,&nbsp;Fuller W. Bazer ,&nbsp;Gregory A. Johnson","doi":"10.1016/j.placenta.2025.06.012","DOIUrl":"10.1016/j.placenta.2025.06.012","url":null,"abstract":"<div><h3>Introduction</h3><div>Secreted protein acidic and rich in cysteine (SPARC) and secreted phosphoprotein 1 (SPP1) are matricellular proteins implicated in many physiological processes including cell migration, tissue remodeling, and angiogenesis. The role of SPP1 in the pregnancies of many species has been studied extensively, however the contributions of SPARC to pregnancy are not well understood.</div></div><div><h3>Methods</h3><div>Here, we examine the localization of Sparc mRNA and protein in mouse fetuses, endometrial decidua, and placentae during various days of pregnancy in the delayed implantation model to identify potential interactions between SPARC and SPP1 in mouse pregnancy.</div></div><div><h3>Results</h3><div>In situ hybridization and immunofluorescence microscopy localized Sparc mRNA and protein to the endometrial stroma at implantation sites on gestational day (GD)5–6, and to the parietal yolk sac, Reichert's membrane, and select trophoblast lineages on GD9-16. Spp1 mRNA is strongly expressed in the endometrial luminal epithelium at inter-implantation sites and SPP1 is detected in uterine natural killer cells (uNKs) and glycogen trophoblast cells of the placenta. SPARC and SPP1 both localized to many regions of the GD16 fetus, including separate chondroblastic lineages within the developing cartilage.</div></div><div><h3>Discussion</h3><div>The placental expression pattern of these two matricellular proteins suggests possible spatio-temporal and mechanistic relationships between SPARC and SPP1 during pregnancy. While future studies are needed to elucidate the functional roles of SPARC, results from this study demonstrate that SPARC is a prominent component of the matricellular milieu throughout gestation.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"168 ","pages":"Pages 125-134"},"PeriodicalIF":3.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single cell RNA-sequencing reveals BHLHE40 and NDRG1 as key regulatory molecules modulating the trophoblastic senescence in preeclampsia","authors":"Yang Zhang ,&nbsp;Jie Chu ,&nbsp;Zhicheng Yu ,&nbsp;Yanping Gong","doi":"10.1016/j.placenta.2025.06.009","DOIUrl":"10.1016/j.placenta.2025.06.009","url":null,"abstract":"<div><h3>Introduction</h3><div>Preeclampsia (PE) is a severe obstetric complication characterized by trophoblastic dysfunction and compromised placental development. Cellular senescence has been notably reported in PE trophoblasts, which contributes to placental dysfunction. However, the underlying molecular mechanisms and key regulatory targets governing this process remain poorly understood.</div></div><div><h3>Methods</h3><div>We analyzed PE-associated single-cell RNA sequencing (GSE173193), ChIP detection (GSE24129), and bulk transcriptome (GSE75010) datasets from GEO database. Through integrated analysis of trophoblast-specific differential expression, weighted gene co-expression network analysis (WGCNA), and senescence-related gene profiles, we identified BHLHE40 and NDRG1 as key regulatory molecules. Their clinical predictive value was comprehensively assessed via the nomogram model. Bioinformatic findings were systematically validated in human PE placentas and LPS-induced PE rat model. The functional effects of BHLHE40 and NDRG1 overexpression on trophoblastic senescence were extensively evaluated <em>in vitro</em>.</div></div><div><h3>Results</h3><div>BHLHE40 and NDRG1 emerged as crucial regulators of trophoblastic senescence in PE, demonstrating significant predictive value for adverse pregnancy outcomes. Both molecules showed consistent aberrant overexpression in human PE placentas and LPS-induced PE rat placentas. Importantly, their overexpression significantly accelerated cellular senescence in trophoblasts <em>in vitro</em>.</div></div><div><h3>Discussion</h3><div>Through integrated bioinformatic analysis and experimental validation, we reveal BHLHE40 and NDRG1 as key regulators of abnormal trophoblast senescence in PE, providing new insights into placental dysfunction mechanisms and potential therapeutic targets in PE management.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"168 ","pages":"Pages 74-87"},"PeriodicalIF":3.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of periconceptional pathology and pathophysiology: Where do we go next?","authors":"James M. Roberts ,&nbsp;Graham J. Burton ,&nbsp;Kirk P. Conrad ,&nbsp;Barbara Luke ,&nbsp;Mellissa RW. Mann ,&nbsp;Ashley Moffett ,&nbsp;McKenzie K. Jancsura","doi":"10.1016/j.placenta.2025.06.010","DOIUrl":"10.1016/j.placenta.2025.06.010","url":null,"abstract":"<div><div>It is becoming increasingly evident that studies of pregnancy and pregnancy complications must begin earlier in pregnancy than has typically been the case. Important events with effects through and beyond pregnancy take place in the periconceptional period, from shortly before to shortly after conception. During this time ovarian hormones act not only to facilitate successful implantation but also direct maternal physiological adaptations necessary for pregnancy. Decidua also undergoes changes necessary for successful implantation of the embryo. These involve not only maternal preparation of a receptive site for implantation but interactions, including immunological and others, of cells of the maternal decidua and embryo. All of this occurs in a setting in which the cells of the embryo are involved in complex epigenetic changes. Assisted reproductive technology (ART) has the potential to influence many of these since ART typically occurs at this time of these epigenetic change. Many of the variables involved in ART have the potential to influence epigenetic adaptation. In fall of 2023 the Global Pregnancy Collaboration brought together experts in these areas to consider the status of our understanding of the periconceptional period. This manuscript presents an update of this information including new approaches to study these very early events. The potential impact of ART on periconceptional events and the mechanisms involved are presented. Based upon this information recommendations are made for future research in these important areas.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"168 ","pages":"Pages 98-110"},"PeriodicalIF":3.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insufficient expression of VTN promotes the development of early-onset severe preeclampsia through the HEY1/autophagy signaling pathway","authors":"Gonghua Qi ,&nbsp;Yanmin Gong ,&nbsp;Yi Li ,&nbsp;Yanhui Jin ,&nbsp;Shuqi Chi ,&nbsp;Wenxia Zhang ,&nbsp;Xia Luo","doi":"10.1016/j.placenta.2025.06.011","DOIUrl":"10.1016/j.placenta.2025.06.011","url":null,"abstract":"<div><h3>Introduction</h3><div>Early-onset severe preeclampsia (eosPE) is a serious pregnancy complication that affects maternal and fetal health. This study aims to identify genes involved in the development of eosPE by analyzing placental tissues from eosPE patients and healthy controls.</div></div><div><h3>Methods</h3><div>We performed Gene Ontology (GO) analysis on differentially expressed genes (DEGs) from the placental tissues of eosPE patients and healthy controls using data from the GEO database. Vitronectin (VTN), a gene identified as relevant in the top three molecular function categories, was further investigated. Functional assays were conducted on HTR8/SVneo cells, HUVECs, and primary extravillous trophoblasts (EVTs). Additionally, transcriptome sequencing was performed to study the impact of VTN overexpression on signaling pathways.</div></div><div><h3>Results</h3><div>VTN was found to be significantly downregulated in eosPE placental tissues compared to healthy controls. Functional assays demonstrated that VTN enhanced migration, invasion, and tube formation in trophoblast cells. VTN overexpression impacted the Notch signaling pathway, upregulating HEY1, a downstream target. HEY1 was also found to be downregulated in eosPE placental tissues. Knockdown of VTN led to increased LC3II expression, indicating enhanced autophagy, while HEY1 overexpression alleviated this effect. Autophagy inhibition with 3-MA partially restored function suppressed by VTN knockdown.</div></div><div><h3>Discussion</h3><div>Our findings suggest that insufficient VTN expression impairs trophoblast cell migration, invasion, and endothelial-like tube formation through the HEY1/autophagy pathway, contributing to eosPE pathogenesis. This highlights VTN's potential role in the development of eosPE and suggests new therapeutic targets for managing this pregnancy complication.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"168 ","pages":"Pages 56-66"},"PeriodicalIF":3.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaporin-1 and aquaporin-4 in human placental angiogenesis: insights into the critical interaction with caveolin-1","authors":"Reppetti Julieta ,&nbsp;Gornalusse Germán ,&nbsp;Etcheverry Tomás ,&nbsp;Farina Mariana ,&nbsp;Damiano Alicia ,&nbsp;Martínez Nora","doi":"10.1016/j.placenta.2025.06.008","DOIUrl":"10.1016/j.placenta.2025.06.008","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"168 ","pages":"Pages 111-123"},"PeriodicalIF":3.0,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety assessment of drugs in pregnancy: An update of pharmacological models.","authors":"Lunbo Tan, Dongni Huang, Huisheng Ge, Ruonan Fan, Xiaoqing Wei, Xiang Feng, Chuting Xu, Wei Zhou, Hongbo Qi","doi":"10.1016/j.placenta.2025.06.007","DOIUrl":"https://doi.org/10.1016/j.placenta.2025.06.007","url":null,"abstract":"<p><p>Pregnant women are largely excluded from clinical drug trials due to ethical concerns, leading to a critical knowledge gap in medication safety during pregnancy. Indeed, over 90 % of approved drugs lack pharmacokinetic data in pregnant patients, and only two new therapies have been specifically developed for use in pregnancy in the past three decades. Traditional pharmacological models (animal studies and static 2D cell cultures) often fail to predict maternal-fetal drug transfer and toxicity due to interspecies differences and an inability to mimic the dynamic physiology of pregnancy-particularly the pivotal role of the placenta. Consequently, clinicians must frequently weigh maternal treatment needs against uncertain fetal risks. Recent technological advances have begun to bridge these gaps: placental organoids and microfluidic placental-on-chip now serve as placental pharmacology platforms, and AI-based predictive models can integrate complex datasets to forecast drug disposition in pregnancy. This mini-review provides an updated overview of these emerging approaches for pregnancy drug safety assessment. It highlights how these innovative models recapitulate key aspects of placental structure and function, enabling more accurate evaluation of drug pharmacokinetics and toxicity at the maternal-fetal interface. Integrating advanced placental models with computational tools offers transformative potential for pregnancy pharmacotherapy. Future efforts should focus on combining experimental models with computational approaches to improve translational relevance. Standardized protocols, clinical biomarker validation, and ethical governance are essential to advance these technologies from experimental platforms to regulatory and clinical decision-making tools.</p>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological glucose levels associated with gestational diabetes impact the human placental transcriptome in an ex vivo model","authors":"Abigail R. Byford ,&nbsp;Katy Walsh ,&nbsp;Dapeng Wang ,&nbsp;Chloe Baird-Rayner ,&nbsp;Virginia Pensabene ,&nbsp;Eleanor M. Scott ,&nbsp;Karen Forbes","doi":"10.1016/j.placenta.2025.06.006","DOIUrl":"10.1016/j.placenta.2025.06.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Gestational diabetes mellitus (GDM) increases the risk of pathological fetal growth, including rates of large-for-gestational age (LGA) infants, which in turn increases the risk of offspring later developing cardiometabolic complications. Recent continuous glucose monitoring (CGM) studies have revealed that temporal periods of mild hyperglycaemia are linked to LGA, and too tight glycaemic control can increase periods of maternal hypoglycaemia and increase the risk of delivering small-for-gestational age (SGA) infants. The underlying mechanisms are unclear but likely involve the placenta.</div></div><div><h3>Methods</h3><div><em>Ex vivo</em> human placental explants from term uncomplicated pregnancies were cultured in varying glucose concentrations for 48 h to recapitulate <em>in vivo</em> maternal glucose profiles. Glucose, osmolality, human chorionic gonadotrophin (hCG) and lactate dehydrogenase (LDH) were measured in conditioned medium, and RNA sequencing performed, followed by functional enrichment analysis (FEA).</div></div><div><h3>Results</h3><div>Medium changes every 6–18 h in variable (5/5.5 mM), or constant 5 mM or 7 mM glucose were appropriate to model maternal normoglycaemia, periods of mild hypoglycaemia and periods of mild hyperglycaemia, respectively. There were 61 differentially expressed genes (DEGs) in explants cultured in mild hyperglycaemic conditions and 54 DEGs in mild hypoglycaemic conditions. FEA revealed that transcripts altered by mild hyperglycaemia were associated with vascular development and lipid metabolism/homeostasis, whilst those altered by mild hypoglycaemia were associated with cell turnover.</div></div><div><h3>Conclusions</h3><div>Together this data demonstrates that subtle changes in maternal glucose impact the placenta and may contribute to altered fetal growth. This highlights the importance of employing CGM in pregnancies complicated by GDM and utilising physiological glucose levels in <em>ex vivo</em>/<em>in vitro</em> placental studies.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"168 ","pages":"Pages 46-55"},"PeriodicalIF":3.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polystyrene microplastics internalization by term placental chorionic villi explants.","authors":"Aldilane Lays Marques, Rodrigo Weingrill, Stephanie Ospina-Prieto, Keyla Pires, Isadora Cavalcante, Ashelley Kettyllem Sousa, Iasmin Cristina Cavalcante, Lais Oliveira, Samuel Souza, Eduardo Jorge Fonseca, Jacob Garcia, Men-Jean Lee, Johann Urschitz, Alexandre Borbely","doi":"10.1016/j.placenta.2025.06.002","DOIUrl":"https://doi.org/10.1016/j.placenta.2025.06.002","url":null,"abstract":"<p><strong>Introduction: </strong>Microplastics (MPs) are pervasive environmental contaminants increasingly found within human tissues, including the placenta. This study explores the potential of polystyrene (PS)-MPs to cross the placental barrier and their general distribution within term placental chorionic villi explants.</p><p><strong>Methods: </strong>Term placental chorionic villi explants were exposed up to 72 h to 100 μg/mL of 5 μm-size polystyrene (PS)-MPs, and their internalization was analyzed by optical microscopy, confocal atomic force microscopy (C-AFM) and fluorescence confocal imaging.</p><p><strong>Results: </strong>The PS-MPs can traverse the placental barrier. Over 72 h of exposure, these particles were not only adsorbed on the surface but also internalized within the syncytiotrophoblast and dispersed through the chorionic villi mesenchyme. Our observations indicate that PS-MPs are found up to 120 μm deep within the villi, suggesting their capability to penetrate deeply into placental tissue. Furthermore, these MPs were surrounded by a thin layer of actin, implying active internalization mechanisms possibly involving macropinocytosis or phagocytosis, although specific pathways in placental tissues remain to be fully elucidated. No evidence of barrier fissures or membrane ruptures was observed, indicating that the internalization process does not disrupt the syncytiotrophoblast barrier integrity.</p><p><strong>Discussion: </strong>This study underscores the urgent need to understand the implications of such internalization and their effects on placental homeostasis. Given the potential for MPs to influence developmental processes adversely, further research is essential to delineate the mechanisms of MP internalization, possible physiological impacts, and the consequences of fetal exposure.</p>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular regulation and functional benefits of trophoblast syncytialization in optimizing maternal-fetal nutrient allocation.","authors":"Qianqian Li, Yuelin Zhu, Xin Yu, Xuan Shao, Yan-Ling Wang","doi":"10.1016/j.placenta.2025.06.003","DOIUrl":"https://doi.org/10.1016/j.placenta.2025.06.003","url":null,"abstract":"<p><p>The placenta governs maternal-fetal nutrient allocation during pregnancy, primarily mediated by placental villi composed of multinucleated syncytiotrophoblasts (STBs) and mononucleated cytotrophoblasts (CTBs) in humans. STBs are formed through cell fusion of CTBs, a process termed syncytialization. The placental villi are bathed in maternal blood sinus, functioning as a critical unit for bidirectional exchange of nutrients and wastes between the mother and fetus, hormone production, and defense against pathogen infection. Trophoblast fusion is regulated by an intricate network of hormones, growth factors, and cytokines, involving mechanisms such as genetic transcription, endogenous retrovirus-derived signaling, epigenetic regulation via DNA methylation or histone modifications, and post-translational modifications including O-GlcNAcylation and ubiquitination. Emerging evidence highlights STBs as nutrient-sensing hubs that adapt transport capacity to maternal nutrient availability. Recent studies demonstrate the activation of macropinocytosis in STBs via mTOR signaling under conditions of nutritional scarcity, synergizing with the multinucleation process to enhance substrate uptake. Metabolomic analyses reveal remarkable suppression of glycolysis and increased fatty acid oxidation in STBs, while appropriate glucose-acetate metabolism epigenetically guides trophoblast cell fate toward syncytium formation. Consequently, metabolic signalings in trophoblasts constitutes a vital physiological mechanism for balancing maternal-placental-fetal nutrient allocation. The integration of interdisciplinary approaches, including single-cell omics, advanced imaging techniques, and organoid models, holds promise for transformative insights into placental biology and the development of diagnostics and therapies targeting placental insufficiency syndromes affecting maternal and fetal health.</p>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}