Placenta最新文献

{"title":"Trophoblastic epithelial-mesenchymal transition with consecutive fibrosis: A cause of reduced placental function in hyperglycemic pregnancies?","authors":"Lara Hausdorf , Silke Große , Anne Rüllich , Alexander Berndt , Nikolaus Gaßler , Tanja Groten","doi":"10.1016/j.placenta.2024.10.042","DOIUrl":"10.1016/j.placenta.2024.10.042","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"160 ","pages":"Page 149"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental transfer of chemicals: Ex vivo placental perfusion model","authors":"Line Mathiesen , Lisbeth E. Knudsen","doi":"10.1016/j.placenta.2024.10.047","DOIUrl":"10.1016/j.placenta.2024.10.047","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"160 ","pages":"Pages 150-151"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenging the Syncytin-1 receptor dualism: Implications for cell fusion in the human placenta","authors":"Kateřina Trejbalová , Kryštof Štafl , Martin Trávníček , Anna Janovská , Jiří Hejnar","doi":"10.1016/j.placenta.2024.10.056","DOIUrl":"10.1016/j.placenta.2024.10.056","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"160 ","pages":"Pages 153-154"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometrial gland-derived extracellular vesicles and their miRNA content","authors":"Molly Rutt , Alexandra J. Kermack , Ka Ying Bonnie Ng , James W. Hounslow , Sarah F. Newbury , Rohan M. Lewis , Ying C. Cheong , Jane K. Cleal","doi":"10.1016/j.placenta.2024.10.040","DOIUrl":"10.1016/j.placenta.2024.10.040","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"160 ","pages":"Pages 148-149"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microphysiological models of the human placenta to study Brucella infections and antibiotic treatment during pregnancy","authors":"Odysseas Chaliotis , Julia A. Boos , Maren Ketterer , Christoph Dehio , Andreas Hierlemann","doi":"10.1016/j.placenta.2024.10.029","DOIUrl":"10.1016/j.placenta.2024.10.029","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"160 ","pages":"Page 145"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of macrophage polarization by metformin through inhibition of TLR4/NF-κB pathway to improve pre-eclampsia","authors":"Wei Shen ,&nbsp;Qingfu Wang ,&nbsp;Guofang Shen ,&nbsp;Meiling Gu ,&nbsp;Qifeng Shen ,&nbsp;Ailan Zhang ,&nbsp;Xiaohong Zhu","doi":"10.1016/j.placenta.2025.01.002","DOIUrl":"10.1016/j.placenta.2025.01.002","url":null,"abstract":"<div><h3>Introduction</h3><div>Pre-eclampsia (PE) is a pregnancy complication featuring hypertension and proteinuria. Metformin exerts clinically preventive effects on PE with an unspecified mechanism.</div></div><div><h3>Methods</h3><div>Placental tissues from PE patients and normal pregnant (NP) women were collected. Twenty-four pregnant mice were divided into control, PE (40 μg/kg lipopolysaccharides (LPS)-induced modeling), aspirin, and metformin groups. After acquisition of bone marrow-derived macrophages (BMDM) and THP-1 cells, cells were categorized into control, LPS (100 ng/mL), metformin, and metformin + toll-like receptor 4 (TLR4) agonist RS 09 groups. Inflammatory factors and macrophage polarization were detected by ELISA, flow cytometry, immunofluorescence, immunohistochemistry, and qRT-PCR methods. TLR4/Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway protein expression was examined using Western blot.</div></div><div><h3>Results</h3><div>Both PE patients and PE-like mice enhanced inducible nitric oxide synthase (iNOS), TLR4, p-NF-κB/NF-κB, and p-inhibitor of NF-κB (IκBα)/IκBα expression, and lower arginase 1 (Arg-1) expression. Moreover, metformin treatment in PE-like mice increased fetal number and weight and reduced hypertension, proteinuria, insulin resistance, tumor necrosis factor-α (TNF-α), IL-6, IL-1β, chemokine ligand 4 (CCL4), C-X-C motif chemokine ligand 2 (CXCL2) expression and M1 macrophage polarization, with similar inhibition to aspirin. In LPS-induced cells, metformin had the same effects mentioned above. Decreased TLR4, p-NF-κB/NF-κB, and p-IκBα/IκBα protein expression was caused by metformin both <em>in vivo</em> and <em>in vitro</em>. <em>In vitro</em>, RS 09 intervention inhibited anti-inflammatory and pro-M2 polarizing effects of metformin, activating TLR4/NF-κB pathway.</div></div><div><h3>Conclusion</h3><div>Metformin may ameliorate PE by promoting M2 macrophage polarization through up-regulating TLR4/NF-κB pathway, laying theoretical basis for metformin clinical application in PE.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"160 ","pages":"Pages 89-99"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet-derived factors modulate gene expression profile of human trophoblasts","authors":"Freya Lyssy ,&nbsp;Désirée Forstner ,&nbsp;Jacqueline Guettler ,&nbsp;Nadja Kupper ,&nbsp;Kaja Ujčič ,&nbsp;Stefan Wernitznig ,&nbsp;Lena Neuper ,&nbsp;Christine Daxboeck ,&nbsp;Amin El-Heliebi ,&nbsp;Julian C. Krappinger ,&nbsp;Julia Feichtinger ,&nbsp;Gerhard Cvirn ,&nbsp;Anna-Lena Hoebler ,&nbsp;Juergen Pollheimer ,&nbsp;Joanna L. James ,&nbsp;Martin Gauster","doi":"10.1016/j.placenta.2024.10.050","DOIUrl":"10.1016/j.placenta.2024.10.050","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"160 ","pages":"Pages 151-152"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring organoid oviductal gland secretions for optimizing in vitro culture media: Insights from a bovine model","authors":"Sergio Navarro-Serna ,&nbsp;Eva González-Cantó ,&nbsp;Jon Romero-Aguirregomezcorta ,&nbsp;Ana Ferrero-Micó ,&nbsp;Maravillas Mellado-López ,&nbsp;Pilar Coy ,&nbsp;Vicente Pérez-García","doi":"10.1016/j.placenta.2024.10.062","DOIUrl":"10.1016/j.placenta.2024.10.062","url":null,"abstract":"","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"160 ","pages":"Pages 155-156"},"PeriodicalIF":3.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Optimal timing for RNA isolation: Recommendations for placenta sample collection under clinical conditions”","authors":"J. Sommer ,&nbsp;L. Ehrlich ,&nbsp;A. Kühn ,&nbsp;K. Schellong ,&nbsp;R.C. Rancourt ,&nbsp;W. Henrich ,&nbsp;T. Braun","doi":"10.1016/j.placenta.2025.01.011","DOIUrl":"10.1016/j.placenta.2025.01.011","url":null,"abstract":"<div><div>In placenta research, tissue quality is essential for following laboratory investigations. In clinical settings with deliveries as an unpredictable factor, delayed sampling or incorrect storage until sampling is a common problem. Therefore, we set out to define time points for different storage conditions where good tissue quality is still achievable. RNA related parameters like 260/280-ratio, RNA integrity number (RIN) and gene expression were analyzed in 172 samples over a 24-h interval. We suggest a cut-off-point of 3-h after delivery of the placenta to ensure good tissue and RNA quality, especially when selecting genes with complex or low mRNA expression levels.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"161 ","pages":"Pages 52-54"},"PeriodicalIF":3.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactate promotes premature aging of preeclampsia placentas through histone lactylation-regulated GADD45A","authors":"Xiang Li ,&nbsp;Qianghua Wang ,&nbsp;Jiaojiao Fei ,&nbsp;Zhixin Jin ,&nbsp;Yue Wu ,&nbsp;Yafen Tao ,&nbsp;Chuanyue Jiang ,&nbsp;Xuegu Wang ,&nbsp;Nana Yang ,&nbsp;Biao Ding ,&nbsp;Chengli Dou","doi":"10.1016/j.placenta.2025.01.010","DOIUrl":"10.1016/j.placenta.2025.01.010","url":null,"abstract":"<div><h3>Background</h3><div>Premature placental aging has been linked to preeclampsia (PE), with lactate identified as a promoter of cellular senescence in various cell types. In this study, we explored the role and underlying mechanisms of lactate in driving premature placental aging associated with PE.</div></div><div><h3>Methods</h3><div>To evaluate senescence markers in placental samples or trophoblast cells, we conducted SA-β-Gal staining, western blotting, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and immunofluorescence assays. SiRNA transfection was used to reduce GADD45A expression in HTR-8/SVneo cells exposed to lactate. Additionally, chromatin immunoprecipitation-qPCR (ChIP-qPCR) was used to analyze histone lactylation at the GADD45A promoter region.</div></div><div><h3>Results</h3><div>SA-β-Gal staining indicated a significant increase in senescent cell proportions in placentas from PE patients compared to controls. Treatment with lactate enhanced senescence in trophoblast cells, leading to an increase in P16 expression. RNA sequencing analysis showed that genes differentially expressed in lactate-treated cells were involved in pathways linked to cellular senescence. Additionally, lactate augmented GADD45A expression and increased histone lactylation at its promoter region, while knocking down GADD45A in trophoblast cells mitigated the senescence induced by lactate.</div></div><div><h3>Conclusions</h3><div>Lactate promotes trophoblast senescence through epigenetic upregulation of GADD45A expression, offering fresh perspectives on the molecular mechanisms and potential treatment targets for PE.</div></div>","PeriodicalId":20203,"journal":{"name":"Placenta","volume":"161 ","pages":"Pages 39-51"},"PeriodicalIF":3.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}