Planta最新文献

{"title":"Identification and characterization of a key gene controlling purple leaf coloration in non-heading Chinese cabbage (Brassica rapa).","authors":"Jia Si, Xiaoqing Zhou, Xinyu Chen, Huilin Ming, Hanqiang Liu, Maixia Hui","doi":"10.1007/s00425-025-04630-3","DOIUrl":"10.1007/s00425-025-04630-3","url":null,"abstract":"<p><strong>Main conclusion: </strong>Chalcone isomerase (BraCHI, BraA03g059660.3C) is the candidate gene controlling purple leaf coloration in non-heading Chinese cabbage. A 10-bp deletion in its promoter enhances gene expression in purple plants, likely by disrupting MYB transcription factor binding, leading to anthocyanin accumulation. Leaf color is a critical trait influencing the commercial and nutritional value of leafy vegetables, with purple-leafed varieties prized for their high anthocyanin content. In this study, we investigated the genetic basis of purple leaf coloration in non-heading Chinese cabbage (Brassica rapa). Using a recombinant inbred line (RIL) population derived from a cross between purple-leafed S45P and green-leafed S45G lines, bulked segregant analysis sequencing (BSA-seq) and fine mapping were performed. The analysis identified BraP2, a locus on chromosome A03 associated with purple leaf coloration. Within the 65.31 kb candidate region, BraA03g059660.3C, encoding chalcone isomerase (CHI), was identified as the strongest candidate gene. Quantitative real-time PCR (qRT-PCR) revealed significantly higher expression of BraA03g059660.3C in purple-leafed S45P plants compared to green-leafed S45G plants. Further sequence analysis uncovered a 10-bp deletion in the promoter region of BraA03g059660.3C in S45P plants. This deletion likely disrupts a MYB transcription factor binding site, enhancing gene expression and promoting anthocyanin accumulation. Our findings demonstrate that BraA03g059660.3C plays a pivotal role in controlling purple leaf coloration in non-heading Chinese cabbage. This discovery advances the understanding of anthocyanin biosynthesis regulation and provides valuable genetic resources for breeding Brassica crops with improved esthetic and nutritional qualities.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"80"},"PeriodicalIF":3.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omics advancements towards exploring arsenic toxicity and tolerance in plants: a review.","authors":"Sayyeda Hira Hassan, Melissa Simiele, Gabriella Stefania Scippa, Domenico Morabito, Dalila Trupiano","doi":"10.1007/s00425-025-04646-9","DOIUrl":"10.1007/s00425-025-04646-9","url":null,"abstract":"<p><strong>Main conclusion: </strong>Omics approaches provide comprehensive insights into plant arsenic stress responses, setting the stage for engineering arsenic-tolerant crops. Understanding arsenic (As) toxicity in plants is crucial for environmental and agricultural sustainability, considering the implications of As in impacting soil productivity and environmental health. Although some articles already examined the detailed molecular mechanisms behind As toxicity and tolerance, a comprehensive review of recent omics advancements in studying plant responses to As exposure is needed. The present review highlights the valuable contribution of omics approaches (genomics, transcriptomics, proteomics, and metabolomics) to characterize the intricate response to As overall, which could empower As-tolerant plant development. Genomic techniques, such as QTL mapping, GWAS, RAPD, and SSH, hold the potential to provide valuable insights into the genetic diversity and expression patterns associated with the plant response to As stress, highlighting also the power of new advanced technology such as CRISPR-Cas9. Transcriptomics approaches (e.g., microarrays and RNA sequencing) revealed gene expression patterns in plants under As stress, emphasizing the role of sulfur metabolism in As tolerance. Proteomics, using 2-DE combined with MALDI-ToF MS or ESI-MS/MS, offers insights into the stress-inducible proteins and their involvement in As toxicity mitigation, while iTRAQ-based proteomics enabled an understanding of cultivar-specific responses under high As concentration. Metabolomics, with LC-MS, GC-MS, (U)HPLC, and NMR, elucidated small molecule alterations and complex metabolic activities occurring under As plant exposure. Compendium of data and evidence-related tools offers a foundation for advancing As-tolerant plant development and promoting environmental and agricultural resilience.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"79"},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge of microalgal Rubiscos helps to improve photosynthetic efficiency of crops.","authors":"Tongtong Zhu, Peng Ning, Yiguo Liu, Min Liu, Jianming Yang, Zhaobao Wang, Meijie Li","doi":"10.1007/s00425-025-04645-w","DOIUrl":"10.1007/s00425-025-04645-w","url":null,"abstract":"<p><strong>Main conclusion: </strong>A comprehensive understanding of microalgal Rubiscos offers opportunities to enhance photosynthetic efficiency of crops. As food production fails to meet the needs of the expanding population, there is increasing concern about Ribulose-1, 5-diphosphate (RuBP) carboxylase/oxygenase (Rubisco), the enzyme that catalyzes CO₂ fixation in photosynthesis. There have been many attempts to optimize Rubisco in crops, but the complex multicellular structure of higher plants makes optimization more difficult. Microalgae have the characteristics of rapid growth, simple structure and easy molecular modification, and the function and properties of their Rubiscos are basically the same as those of higher plants. Research on microalgal Rubiscos helps to broaden the understanding of Rubiscos of higher plants. Also, transferring all or part of better microalgal Rubiscos into crop cells or giving crop Rubiscos the advantages of microalgal Rubiscos can help improve the photosynthesis of crops. In this review, the distribution, origin, evolution, molecular structure, folding, assembly, activation and kinetic properties of microalgal Rubiscos are summarized. Moreover, the development of some effective methods to improve the properties and application of Rubiscos in microalgae are also described.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"78"},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PpSPL1 and PpSPL15 inhibit peach branching by increasing strigolactone synthesis.","authors":"Wan Pei, Jie Zhang, Ruixian Shen, Hefang Xie, Yajia Zhang, Junjie Zhang, Xiaodong Lian, Haipeng Zhang, Nan Hou, Lei Wang, Xianbo Zheng, Jun Cheng, Wei Wang, Xia Ye, Jidong Li, Xiaobei Wang, Jiancan Feng, Bin Tan","doi":"10.1007/s00425-025-04659-4","DOIUrl":"10.1007/s00425-025-04659-4","url":null,"abstract":"<p><strong>Main conclusion: </strong>PpSPL1 and PpSPL15 inhibit peach branching by directly binding to and upregulating the expression of strigolactone (SL) synthesis gene PpLBO1. Branch number is a crucial agronomic trait that influences tree architecture, directly affecting fruit yield and quality. It remains unknown whether SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL), an important transcription factor in determining plant architecture, is involved in the peach branching process. In this study, we found that PpSPL1 and PpSPL15 exhibited significantly higher expression levels in pillar type peach 'Sahonglongzhu' (with fewer secondary branches) compared to standard-type peach 'Okubo' (with more secondary branches). PpSPL1 and PpSPL15 can directly bind to the promoter of the SL synthesis gene PpLBO1. Transient overexpression of PpSPL1 and PpSPL15 in 'Sahonglongzhu' peach axillary buds significantly increased the expression of PpLBO1 and endogenous SL content. Conversely, opposite results were obtained when the expression of PpSPL1 and PpSPL15 was transiently silenced in peach axillary buds. Gene function analysis indicated that transient overexpression of PpSPL1 and PpSPL15 in peach seedlings clearly inhibited peach branching. On the contrary, the number of branches dramatically increased when the expression of PpSPL1 and PpSPL15 were transiently silenced in peach seedlings. These results suggested that PpSPL1 and PpSPL15 could bind to and enhance the expression of PpLBO1, further inhibiting peach branching.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"77"},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing sweet potato production: a comprehensive analysis of the role of auxins and cytokinins in micropropagation.","authors":"Gideon Adu Donyina, Adrienn Szarvas, Vincent Agyemang Opoku, Edit Miko, Melinda Tar, Szilárd Czóbel, Tamás Monostori","doi":"10.1007/s00425-025-04650-z","DOIUrl":"10.1007/s00425-025-04650-z","url":null,"abstract":"<p><strong>Main conclusion: </strong>This review emphasizes the prevalent auxins and cytokinins used in sweet potato micropropagation, their optimal concentrations for effective in vitro regeneration, various propagation techniques, and Africa's potential to improve sweet potato production. Ipomoea batatas (L.) Lam., or sweet potato, is a robust, nutritious, and adaptable crop traditionally propagated through conventional methods. These techniques, however, have limitations, prompting the adoption of micropropagation as an efficient alternative for producing healthy, cost-effective plantlets in reduced time. This review critically evaluates the influence of auxins and cytokinins, the most frequently utilized plant growth regulators (PGRs), in enhancing sweet potato micropropagation protocols. The study examines the crop's origins, distribution, and cultivation practices, as well as the morphophysiological effects of PGRs on sweet potatoes. Our analysis reveals that 6-benzylaminopurine (BAP) and N6-benzyladenine (BA) are the predominant cytokinins, while naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) are the primary auxins employed in sweet potato micropropagation. The review also proposes strategies for increasing production, particularly in Africa, and identifies areas requiring further investigation to better understand how these growth regulators impact the physiological development and response of sweet potatoes to environmental stress. This comprehensive assessment contributes to the expanding knowledge base on sweet potato micropropagation and offers valuable insights for researchers and practitioners in the field.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"74"},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interacting effects of crop domestication and soil resources on leaf and root functional traits.","authors":"Victoria Nimmo, Gurcharn S Brar, Adam R Martin, Marney E Isaac","doi":"10.1007/s00425-025-04635-y","DOIUrl":"10.1007/s00425-025-04635-y","url":null,"abstract":"<p><strong>Main conclusion: </strong>Domestication altered wheat leaf functional trait expression, and soil amendments altered root trait expression. These alterations shape crop suitability to stressed environments, and informs variety selection for agronomic conditions. Crop traits have been altered through domestication, resulting in syndromes that assist modern crops in contending with environmental constraints. Yet, we have limited understanding of how domestication has shaped the ability of crops to alter leaf and root functional traits for optimal performance under contemporary agronomic conditions, such as water limitation and organic amendments. We used a greenhouse pot experiment that included a wild progenitor of wheat (Aegilops tauschii), three domesticated wheat (Triticum aestivum) varieties (Watkins, Red Fife and Marquis), and three modern wheat varieties (developed from 1969 to 2016) to assess the effects of domestication on crop functional traits under water limitation and under organic and inorganic soil amendments, and to evaluate how this trait expression moderates rhizosphere soil conditions. Leaf functional trait expression varied significantly across wheat domestication classes, with these differences being almost independent of soil amendment or watering treatments. The wild progenitor expressed resource conservative leaf trait values, with low water use efficiency and stomatal conductance. Root trait expression was influenced by both soil amendment and watering treatment, with all wheat lineages expressing acquisitive traits, e.g., higher specific root length and lower root diameter, under organic amendments. Soil amendments and watering treatments impacted rhizosphere conditions, including microbial diversity and acid phosphatase activity, and domestication class impacted fungal diversity. Broadly, domestication altered the expression of wheat leaf functional traits, and soil amendments altered the expression of wheat root functional traits. These alterations in trait expression and rhizosphere soil response shape crop suitability to drought-prone or nutrient stressed environments, and should be considered when selecting varieties for hybridization for contemporary agronomic conditions.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"75"},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of anthocyanins' composition and regulatory genes involved in anthocyanins biosynthesis in water dropwort (Oenanthe javanica).","authors":"Kai Feng, Nan Sun, Ya-Qing Bian, Wu-Di Rui, Ya-Jie Yan, Zhi-Yuan Yang, Jia-Lu Liu, Zi-Qi Zhou, Shu-Ping Zhao, Peng Wu, Liang-Jun Li","doi":"10.1007/s00425-025-04660-x","DOIUrl":"10.1007/s00425-025-04660-x","url":null,"abstract":"<p><strong>Main conclusion: </strong>This study showed that anthocyanin was the main pigments related to purple stem and OjUFGT1 is involved in anthocyanin glycosylation in water dropwort. Water dropwort is a kind of aquatic vegetable with many medicinal values. In the study, the green-stem water dropwort 'FQ1H' and purple-stem water dropwort 'Sq013' were selected as plant materials. The anthocyanins composition was determined by UPLC-MS/MS and the transcript profile was analyzed based on RNA-seq in water dropwort. Nine anthocyanins were identified from water dropwort by UPLC-MS/MS. Petunidin and anthocyanin have higher content, which play a crucial role in the formation of purple stem. In total, 20,478 DEGs were identified in the purple stem, which might have a high correlation with anthocyanin accumulation. The expressions of 26 DEGs encoding anthocyanin biosynthesis structural genes were determined. Furthermore, co-expression analysis indicated that many R2R3-MYB and bHLH transcription factors were potentially involved in anthocyanin biosynthesis. In vitro enzyme activity assay showed that glycosyltransferase OjUFGT1 recognizes UDP-galactose as glycosyl donor and converts cyanidin to cyanidin-3-O-galactoside. In summary, these results may facilitate the development of our breeding and utilization for the high-anthocyanin water dropwort in the future.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"76"},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-mediated efficient PlCYP81Q38 mutagenesis in Phryma leptostachya.","authors":"Yakun Pei, Wenhan Cao, Xiangchenxi Kong, Shaokang Wang, Zhongjuan Sun, Yayun Zuo, Zhaonong Hu","doi":"10.1007/s00425-025-04657-6","DOIUrl":"10.1007/s00425-025-04657-6","url":null,"abstract":"<p><strong>Main conclusion: </strong>Combined with hairy root transformation, the CRISPR/Cas9 system was established to initiate targeted mutagenesis of PlCYP81Q38, which influenced lignan accumulation in Phryma leptostachya. Phryma leptostachya is a traditional Chinese medicinal herb renowned for its applications in both conventional medicine and natural botanical insecticides, with lignans as the main active ingredients. During the biosynthesis of lignans, PlCYP81Q38, a P450 protein, is assumed to play a crucial role and is accountable for the production of sesamin from (+)-pinoresinol. As a cutting-edge genome editing tool, the CRISPR/Cas9 system is widely employed across diverse species for gene functional research but yet to be harnessed in P. leptostachya. This study utilized the CRISPR/Cas9 system in conjunction with hairy root transformation to initiate targeted mutagenesis in PlCYP81Q38 gene. Employing binary vectors, pYLCRISPR/Cas9Pubi-H, complemented by dual single-stranded guided RNAs (sgRNAs), enabled precise editing at two gene sites and the deletion of large fragments. This editing system resulted in mutagenesis rates surpassing 79%, achieving a notable rate of 61.9% fragment deletion mutants. Liquid chromatography/tandem mass spectrometry confirmed the impact on lignan biosynthesis by PlCYP81Q38-targeted mutagenesis, resulting in the accumulation of pinoresinol and disrupted production of sesamin, 6-demethoxy-leptostachyol acetate, and leptostachyol acetate. Furthermore, the knockout of PlCYP81Q38 up-regulated its upstream pathway genes, such as dirigent gene, cinnamoyl-CoA reductase genes, cinnamyl-alcohol dehydrogenase genes, and p-coumarate 3-hydroxylase genes, identified through gene co-expression analysis. Collectively, mediated by the CRISPR/Cas9 platform, the new biotechnology for targeted genome editing within P. leptostachya, our findings affirm the significant roles of PlCYP81Q38 in the lignan biosynthesis pathway and highlight the potential of CRISPR/Cas9 in exploring the functional genome and secondary metabolite biosynthesis of this plant species.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"73"},"PeriodicalIF":3.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptomics of susceptible and resistant Cucumis metuliferus upon Meloidogyne incognita infection.","authors":"Xiaoxiao Xie, Junru Lu, Runmao Lin, Jian Ling, Zhenchuan Mao, Jianlong Zhao, Qihong Yang, Shijie Zheng, Yan Li, Richard G F Visser, Yuling Bai, Bingyan Xie","doi":"10.1007/s00425-025-04649-6","DOIUrl":"10.1007/s00425-025-04649-6","url":null,"abstract":"<p><strong>Main conclusion: </strong>Comparative transcriptomics has identified several candidate genes contributing to the resistance of Cucumis metuliferus against Meloidogyne incognita. The Southern root-knot nematode (Meloidogyne incognita) is a significant threat to Cucurbitaceae crops. The African horned melon (Cucumis metuliferus), a wild relative, exhibits high resistance to this nematode. To explore the resistance mechanism, phenotypic analyses were conducted on a susceptible inbred line (CM27) and a resistant inbred line (CM3). CM3 exhibited enhanced root biomass and significantly higher resistance compared to CM27, with poor nematode development observed in CM3 roots. Transcriptomic profiling at multiple post-infection time points revealed 2243 and 3700 differentially expressed genes (DEGs) in CM3 and CM27, respectively. Among these, the top ten DEGs upregulated exclusively in CM3 were functionally analyzed using virus-induced gene silencing (VIGS). Silencing of EVM0019904 or EVM0017058 in CM3 led to susceptibility to M. incognita. These findings provide novel insights into the resistance mechanisms of M. incognita in C. metuliferus and offer potential resources for breeding nematode-resistant Cucurbitaceae crops.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"72"},"PeriodicalIF":3.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assignment of low-molecular-weight selenometabolites in the root section of white cabbage.","authors":"Áron Soós, Béla Kovács, Tünde Takács, Márk Rékási, Péter Dobosy, Csaba Szőke, Mihály Dernovics, Péter Ragályi","doi":"10.1007/s00425-025-04651-y","DOIUrl":"10.1007/s00425-025-04651-y","url":null,"abstract":"<p><strong>Main conclusion: </strong>Quantitative and qualitative selenium speciation analyses of the root of white cabbage reveal the presence of elemental Se, selenate, selenomethionine and deaminated derivatives of selenohomolanthionine. White cabbage (Brassica oleracea convar. capitata var. alba) is one of the most consumed vegetable brassicas of the Brassica oleracea species whose production is compatible with the recent strip-till and no-till type farming policies. White cabbage has been in the focus of selenium research for decades as a possible source of food-derived selenium supplementation; however, the root section of the plant has hardly been targeted, being a by-product that is left in or plowed into the soil to serve as an organic fertilizer. The root of selenium-enriched white cabbage, planted on three different soil types (sand, silty sand, and silt), was analyzed for selenium speciation with the complementary use of liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS) and electrospray ionization high-resolution mass spectrometry (LC-ESI-HR-MS) methods after orthogonal (anion/cation exchange) chromatographic purification. Elemental selenium (Se⁰) was the major selenospecies in all cases, accounting for 28-43% of total selenium content. Water and proteolytic extractions could recover a median of 28% of total selenium through the quantification of selenate and selenomethionine, leaving a series of selenocompounds unassigned. Among these latter species, accounting for up to an additional 6% of total selenium, eight low-molecular-weight selenocompounds were detected; five out of the eight compounds could be tentatively identified as deaminated derivatives of selenohomolanthionine.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"71"},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}