Planta最新文献

{"title":"Overexpression of OsSTP1 increases grain yield via enhancing carbohydrate metabolism and transport in rice.","authors":"Dong Liu, Ming-Juan Li, Jin-Song Luo, Hai-Fei Chen, Yong Yang, Gui Xiao, Jun Wu, Abdelbagi M Ismail, Zhen-Hua Zhang","doi":"10.1007/s00425-024-04579-9","DOIUrl":"https://doi.org/10.1007/s00425-024-04579-9","url":null,"abstract":"<p><strong>Main conclusion: </strong>Overexpression of OsSTP1 enhances the non-structural carbohydrate remobilization in the source, starch accumulation in grains, and the transportation of carbohydrates from source to sink during the filling stage. The sugar transporter protein (STP) is the best-characterized subfamily of the monosaccharide transporter (MST) family and plays critical roles in regulating plant stress tolerance, growth, and development. However, the role of STPs in regulating rice yield is poorly understood. In this study, we report that compared with Taipei 309, overexpression of OsSTP1 can achieve higher rice yield. We demonstrate that OsSTP1 mRNA levels are higher than those of the other seven STPs in mixed samples of leaf sheaths, stems, and nodes at 12 days after pollination (DAP). OsSTP1 is prominently expressed in the leaf sheaths, stems, and nodes at the grain filling stage. Subcellular localization analysis revealed that OsSTP1 is localized in the plasma membrane. Overexpression of OsSTP1 increased the activities of amylase (AMY) and sucrose phosphate synthase (SPS) in mixed samples of leaf sheaths, stems, and nodes at 12 DAP, the sucrose content of the phloem exudate, and accumulation of soluble sugars and starch in panicles, ultimately increasing seed-setting rates and grain yields in the Taipei 309 cultivar. These findings indicate that overexpression of OsSTP1 can improve grain yield by synergistically promoting non-structural carbohydrate (NSC) remobilization and transportation.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"5"},"PeriodicalIF":3.6,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Western corn rootworm resistance in maize persists in the absence of jasmonic acid.","authors":"John M Grunseich, Pei-Cheng Huang, Julio S Bernal, Michael Kolomiets","doi":"10.1007/s00425-024-04580-2","DOIUrl":"https://doi.org/10.1007/s00425-024-04580-2","url":null,"abstract":"<p><strong>Main conclusion: </strong>Larva growth, survival, and development speed were not affected by the absence of jasmonic acid (JA) indicating that JA does not have a direct role in maize resistance to western corn rootworm. Jasmonic acid (JA) is a plant hormone that regulates multiple physiological processes including defense against herbivory by chewing insects. Previous research showed its importance for resistance to aboveground herbivory. While few studies have investigated the role of JA in resistance to belowground root-feeding herbivores, none has directly tested the role of JA in such resistance. In this study, we used an opr7opr8 double mutant to directly test the role of JA in resistance to western corn rootworm (Diabrotica virgifera virgifera, WCR), a devastating and specialist pest of maize. The opr7opr8 double mutant is deficient in JA accumulation as we found that it does not accumulate JA nor JA-Ile independently of exposure to WCR. We found no significant difference in growth (body mass), survival, and development of WCR larvae in response to JA deficiency, suggesting that disruption of JA biosynthesis does not impact resistance in maize roots to WCR. Additionally, we observed no significant effect on loss of root tissue caused by WCR associated with JA deficiency, while we found a reduction in shoot growth (mass) associated with WCR herbivory in the opr7opr8 mutant that was not observed in the wildtype. This suggested a role for JA in aboveground growth response to WCR herbivory rather than resistance to WCR.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"6"},"PeriodicalIF":3.6,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broomrape-host interaction: host morphology and physiology as metrics for infestation.","authors":"Amnon Cochavi","doi":"10.1007/s00425-024-04581-1","DOIUrl":"10.1007/s00425-024-04581-1","url":null,"abstract":"<p><strong>Main conclusion: </strong>In contrast to other plant pests, broomrape, parasitic plant, rely on maintaining the productivity of the host plant to complete their life cycle. Parasitic plants, particularly those in the Orobanchaceae family, rely on their host plants to complete their life cycle. Unlike other plant parasites such as fungi and bacteria, which exploit their hosts regardless of their physiological status, parasitic plants development is linked to the host productivity due to their mutual physiological dependence on water availability and sugar metabolism. Presently, most research focuses on the damage caused to the host after the parasite completes its life cycle, including inflorescence emergence and seed dispersal. However, the interaction between parasite and host begins long before these stages. This implies that certain physiological adaptations are necessary to sustain the parasite's development while maintaining the host's productivity. In this review, I compile existing knowledge regarding changes in host physiology during the early developmental stages of parasitic plants, spanning from attachment to inflorescence emergence. Additionally, I highlight knowledge gaps that should be addressed to understand how hosts sustain themselves throughout extended periods of parasitism.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"4"},"PeriodicalIF":3.6,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of CLE peptide signaling in root-knot nematode parasitism of plants.","authors":"Saba Fatima, Saeeda Zaima Zeb, Moh Tariq, Yasar Nishat, Heba I Mohamed, Mansoor A Siddiqui","doi":"10.1007/s00425-024-04576-y","DOIUrl":"10.1007/s00425-024-04576-y","url":null,"abstract":"<p><strong>Main conclusion: </strong>We summarize recent findings that have provided new insights into the mechanisms underlying CLE signaling systems in the regulation of plant development and phytonematode interactions. CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides are short sequences consisting of 12 or 13 amino acids characterized by hydroxylated proline residues, and their presence has been demonstrated in various plant species and phytonematodes across multiple paralogous genes. Here, we review recently conducted research to understanding the signaling pathway of CLE peptide during plant development and infection caused by phytonematodes. Cell-to-cell communication is important for the coherent functioning of living organisms. CLE peptides combined with their specific transmembrane receptors to induce downstream intracellular signaling pathways shows divergent modes of action in many developmental processes in variable species. Moreover, CLE peptide was also involved in plant disease mechanism caused by various plant parasitic nematodes.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"3"},"PeriodicalIF":3.6,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering cold resilience: implementing gene editing tools for plant cold stress tolerance.","authors":"Khushbu Kumari, Suman Gusain, Rohit Joshi","doi":"10.1007/s00425-024-04578-w","DOIUrl":"10.1007/s00425-024-04578-w","url":null,"abstract":"<p><strong>Main conclusion: </strong>This paper highlights the need for innovative approaches to enhance cold tolerance. It underscores how genome-editing tools can deepen our understanding of genes involved in cold stress. Cold stress is a significant abiotic factor in high-altitude regions, adversely affecting plant growth and limiting crop productivity. Plants have evolved various mechanisms in response to low temperatures that enable resistance at both physiological and molecular levels during chilling and freezing stress. Several cold-inducible genes have been isolated and characterized, with most playing key roles in providing tolerance against low-temperature stress. However, many plants fail to survive at low temperatures due to the absence of cold acclimatization mechanisms. Conventional breeding techniques, such as inter-specific or inter-genic hybridization, have had limited effectiveness in enhancing the cold resistance of essential crops. Thus, it is crucial to develop crops with improved adaptability, high yields and resistance to cold stress using advanced genomic approaches. The current availability of gene editing tools offers the opportunity to introduce targeted modifications in plant genomes efficiently, thereby developing cold-tolerant varieties. This review discusses advancements in gene editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)/Cas12a(Cpf1), prime editing (PE) and retron library recombineering (RLR). We focus specifically on the CRISPR/Cas system, which has garnered significant attention in recent years as a groundbreaking tool for genome editing across various species. These techniques will enhance our understanding of molecular interactions under low-temperature stress response and highlight the progress of genome editing in designing future climate-resilient crops.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"2"},"PeriodicalIF":3.6,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PpMYB10.1 regulates peach fruit starch degradation by activating PpBAM2.","authors":"Jieyu Dai, Zhouheng Fang, Jingwen Zhu, Xuyang Zheng, Qianjin Zhan, Lijun Cao, Yanan Hu, Caiping Zhao","doi":"10.1007/s00425-024-04575-z","DOIUrl":"10.1007/s00425-024-04575-z","url":null,"abstract":"<p><p>Starch degradation, a crucial source for soluble sugar, significantly influences fruit flavor development during ripening. Key enzymes in this process include α-amylases (AMYs) and β-amylases (BAMs). In this study, we identified 5 PpAMYs and 9 PpBAMs in peach and categorized them into three and four groups, respectively, based on the gene structures and the phylogenetic analysis. Subsequent expression analysis revealed that elevated levels of PpAMY1, PpAMY5, and PpBAM2 were detected in the middle and late stages of fruit development, suggesting their positive involvement in starch degradation during peach fruit ripening. Transient overexpression experiments conducted in peach fruits and callus further demonstrated that overexpression of PpBAM2 significantly reduced starch content, indicating its important role in starch degradation during peach fruit ripening. Furthermore, we identified a R2R3-MYB transcription factor, PpMYB10.1, which activated the expression of PpBAM2 through the direct interacting with its promoter. In addition, transient overexpression of PpMYB10.1 could significantly reduce starch content in peach callus. Consequently, our findings highlight the positive role of PpBAM2 in peach starch degradation, with PpMYB10.1 serving as an activator during this process.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"1"},"PeriodicalIF":3.6,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of the antimalarial drug precursor amorphadiene by microbial terpene synthase-like from the moss Sanionia uncinata.","authors":"Hyeonjin Kim, Yelim Lee, Jihyeon Yu, Jong-Yoon Park, Jungeun Lee, Sang-Gyu Kim, Youbong Hyun","doi":"10.1007/s00425-024-04558-0","DOIUrl":"10.1007/s00425-024-04558-0","url":null,"abstract":"<p><strong>Main conclusion: </strong>The microbial terpene synthase-like of the moss Sanionia uncinata displays the convergent evolution of a rare plant metabolite amorpha-4,11-diene synthesis. Despite increasing demand for the exploration of biological resources, the diversity of natural compounds synthesized by organisms inhabiting various climates remains largely unexplored. This study focuses on the moss Sanionia uncinata, known as a predominant species within the polar climates of the Antarctic Peninsula, to systematically explore its metabolic profile both in-field and in controlled environments. We here report a diverse array of moss-derived terpene volatiles, including the identification of amorpha-4,11-diene, a rare sesquiterpene compound that is a precursor for antimalarial drugs. Phylogenetic reconstruction and functional validation in planta and in vitro identified a moss terpene synthase, S. uncinata microbial terpene synthase-like 2 (SuMTPSL2), which is associated with amorpha-4,11-diene production. We demonstrate that expressing SuMTPSL2 in various heterologous systems is sufficient to produce amorpha-4,11-diene. These results highlight the metabolic diversity in Antarctica, but also provide insights into the convergent evolution leading to the synthesis of a rare plant metabolite.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"260 6","pages":"145"},"PeriodicalIF":3.6,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The origin and metabolic fate of 4-hydroxybenzoate in Arabidopsis.","authors":"Zhaniya Batyrshina, Anna K Block, Gilles J Basset","doi":"10.1007/s00425-024-04572-2","DOIUrl":"10.1007/s00425-024-04572-2","url":null,"abstract":"<p><strong>Main conclusion: </strong>The contribution of p-coumarate β-oxidation and kaempferol cleavage to the pools of glycosylated, free and cell wall-bound 4-hydroxybenzoate is organ-dependent in Arabidopsis. 4-Hydroxybenzoate (4-HB) is a vital precursor for a number of plant primary and specialized metabolites, as well as for the assembly of the plant cell wall. In Arabidopsis, it is known that 4-HB is derived independently from phenylalanine and tyrosine, and that the metabolism of phenylalanine into 4-HB proceeds via at least two biosynthetic routes: the β-oxidation of p-coumarate and the peroxidative cleavage of kaempferol. The precise contribution of these precursors and branches to 4-HB production, however, is not known. Here, we combined isotopic feeding assays, reverse genetics, and quantification of soluble (i.e., free and glycosylated) and cell wall-bound 4-HB to determine the respective contributions of phenylalanine, tyrosine, β-oxidation of p-coumarate, and peroxidative cleavage of kaempferol to 4-HB biosynthesis in Arabidopsis tissues. Over 90% of 4-HB was found to originate from phenylalanine in both leaves and roots. Soluble 4-HB level varied significantly between organs, while the proportion of cell wall-bound 4-HB was relatively constant. In leaves and flowers, glycosylated and cell wall-bound 4-HB were the most and least abundant forms, respectively. Flowers displayed the highest specific content of 4-HB, while free 4-HB was not detected in roots. Although p-coumarate β-oxidation and kaempferol catabolism were found to both contribute to the supply of 4-HB in all tissues, the proportion of kaempferol-derived 4-HB was higher in roots than in leaves and flowers. Within the β-oxidative branch, p-coumaroyl-CoA ligase 4-CL8 (At5g38120) bore a preponderant role in the production of soluble and cell wall-bound 4-HB in leaves, while p-coumaroyl-CoA ligase At4g19010 appeared to control the biosynthesis of soluble 4-HB in flowers. Furthermore, analysis of a series of Arabidopsis T-DNA mutants corresponding to the three major UDP-glucosyltransferases known to act on 4-HB in vitro (UGT75B1, UGT89B1, and UGT71B1) showed that none of these enzymes appeared in fact to have a significant role in the glycosylation of 4-HB in vivo.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"260 6","pages":"144"},"PeriodicalIF":3.6,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput root phenotyping and association analysis identified potential genomic regions for phosphorus use efficiency in wheat (Triticum aestivum L.).","authors":"Vijay Rajamanickam, Amitha Mithra Sevanthi, Stéphanie M Swarbreck, Santosh Gudi, Nisha Singh, Vikas Kumar Singh, Tally I C Wright, Alison R Bentley, Mehanathan Muthamilarasan, Adhip Das, Viswanathan Chinnusamy, Renu Pandey","doi":"10.1007/s00425-024-04577-x","DOIUrl":"10.1007/s00425-024-04577-x","url":null,"abstract":"<p><strong>Main conclusion: </strong>Association analysis identified 77 marker-trait associations (MTAs) for PUE traits, of which 10 were high-confidence MTAs. Candidate-gene mining and in-silico expression analysis identified 13 putative candidate genes for PUE traits. Bread wheat (Triticum aestivum L.) is a major cereal crop affected by phosphorus (P) deficiency, which affects root characteristics, plant biomass, and other attributes related to P-use efficiency (PUE). Understanding the genetic mechanisms of PUE traits helps in developing bread wheat cultivars that perform well in low-P environments. With this objective, we evaluated a bread wheat panel comprising 304 accessions for 14 PUE traits with high-throughput phenotyping under low-P and optimum-P treatments and observed a significant genetic variation among germplasm lines for studied traits. Genome-wide association study (GWAS) using 14,025 high-quality single-nucleotide polymorphisms identified 77 marker-trait associations (MTAs), of which 10 were chosen as high-confidence MTAs as they had > 10% phenotypic variation with logarithm of odds (LOD) scores of more than five. Candidate-gene (CG) mining from high-confidence MTAs identified 180 unique gene models, of which 78 were differentially expressed (DEGs) with at least twofold change in expression under low-P over optimum-P. Of the 78-DEGs, 13 were thought to be putative CGs as they exhibited functional relevance to PUE traits. These CGs mainly encode for important proteins and their products involved in regulating root system architecture, P uptake, transport, and utilization. Promoter analysis from 1500 bp upstream of gene start site for 13 putative CGs revealed the presence of light responsive, salicylic-acid responsive, gibberellic-acid (GA)-responsive, auxin-responsive, and cold responsive cis-regulatory elements. High-confidence MTAs and putative CGs identified in this study can be employed in breeding programs to improve PUE traits in bread wheat.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"260 6","pages":"142"},"PeriodicalIF":3.6,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homoplasy in the embryonic development of terrestrial and epiphytic orchids from the subtribe Malaxidinae (Orchidaceae).","authors":"Galina L Kolomeitseva, Andrey S Ryabchenko, Alexander V Babosha, Vladimir A Koval","doi":"10.1007/s00425-024-04569-x","DOIUrl":"10.1007/s00425-024-04569-x","url":null,"abstract":"<p><strong>Main conclusion: </strong>During evolution, similar vectors of adaptive radiation may have evolved in the subtribe Malaxidinae. This was manifested in homologous series of variability in suspensor shape and seed coat ultrasculpture in clades of terrestrial and epiphytic orchids. The present study examines the variability of embryonic traits across clades and subclades of subtribe Malaxidinae (Orchidaceae), previously identified by molecular genetic data. Ovules and seeds from fruits of orchids of the genera Crepidium, Liparis (sections Cestichis and Blepharoglossum), Dienia, and Oberonia were examined by confocal laser microscopy with fluorescent dye staining. The branched or rounded suspensor in the studied species was unicellular and originated from the nondividing basal cell cb. The elongated or flattened transmission structure of one or two cells was located at the junction of the suspensor and the embryo proper. Two species (Oberonia gammiei and Liparis elliptica) were found to have unitegmal ovules. Three morphological groups of seeds were identified based on the shape and sculpture of the periclinal cell wall. A comparative analysis of the embryological characters in Malaxidinae species reveals that the lobed suspensor is a homoplasy present in different subclades of terrestrial and epiphytic orchids. The flat transmission cell is an apomorphy in the Cestichis subclade. Similarly, the independent formation of the unitegmal ovule occurred in two subclades of epiphytic orchids. The results of our study suggest that similar adaptive radiation vectors may have evolved in the subtribe Malaxidinae in the orchids we studied.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"260 6","pages":"143"},"PeriodicalIF":3.6,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}