Planta最新文献_第2页

Catalysts for sulfur: understanding the intricacies of enzymes orchestrating plant sulfur anabolism.

IF 3.6 3区生物学

Planta Pub Date : 2024-12-17 DOI: 10.1007/s00425-024-04594-w

Ziyue Xu, Dun Liu, Jiadong Zhu, Jiayi Zhao, Shenghai Shen, Yueduo Wang, Pei Yu

{"title":"Catalysts for sulfur: understanding the intricacies of enzymes orchestrating plant sulfur anabolism.","authors":"Ziyue Xu, Dun Liu, Jiadong Zhu, Jiayi Zhao, Shenghai Shen, Yueduo Wang, Pei Yu","doi":"10.1007/s00425-024-04594-w","DOIUrl":"10.1007/s00425-024-04594-w","url":null,"abstract":"Main conclusion: This review highlights the sulfur transporters, key enzymes and their encoding genes involved in plant sulfur anabolism, focusing on their occurrence, chemistry, location, function, and regulation within sulfur assimilation pathways. Sulfur, a vital element for plant life, plays diverse roles in metabolism and stress response. This review provides a comprehensive overview of the sulfur assimilation pathway in plants, highlighting the intricate network of enzymes and their regulatory mechanisms. The primary focus is on the key enzymes involved: ATP sulfurylase (ATPS), APS reductase (APR), sulfite reductase (SiR), serine acetyltransferase (SAT), and O-acetylserine(thiol)lyase (OAS-TL). ATPS initiates the process by activating sulfate to form APS, which is then reduced to sulfite by APR. SiR further reduces sulfite to sulfide, a crucial step that requires significant energy. The cysteine synthase complex (CSC), formed by SAT and OAS-TL, facilitates the synthesis of cysteine, thereby integrating serine metabolism with sulfur assimilation. The alternative sulfation pathway, catalyzed by APS kinase and sulfotransferases, is explored for its role in synthesizing essential secondary metabolites. This review also delves into the regulatory mechanism of these enzymes such as environmental stresses, sulfate availability, phytohormones, as well as translational and post-translational regulations. Understanding the key transporters and enzymes in sulfur assimilation pathways and their corresponding regulation mechanisms can help researchers grasp the importance of sulfur anabolism for the life cycle of plants, clarify how these enzymes and their regulatory processes are integrated to balance plant life systems in response to changes in both external conditions and intrinsic signals.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"16"},"PeriodicalIF":3.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complexity of kodo millet: genomic analysis and implications in crop improvement. 科多小米的复杂性：基因组分析及其对作物改良的影响。

IF 3.6 3区生物学

Planta Pub Date : 2024-12-16 DOI: 10.1007/s00425-024-04588-8

Pratikshya Mishra, Sourav Ranjan Nanda, Tanya Barpanda, Manasi Dash, Suman Dash, Suman Choudhury, Sarojini Roul, Abinash Mishra

{"title":"The complexity of kodo millet: genomic analysis and implications in crop improvement.","authors":"Pratikshya Mishra, Sourav Ranjan Nanda, Tanya Barpanda, Manasi Dash, Suman Dash, Suman Choudhury, Sarojini Roul, Abinash Mishra","doi":"10.1007/s00425-024-04588-8","DOIUrl":"10.1007/s00425-024-04588-8","url":null,"abstract":"Main conclusion: This article explores possible future initiatives, such as the development of targeted breeding and integrated omics approach to boost kodo millet production, nutritional value, and environmental adaptation. Kodo millet is grouped under the genus Paspalum and family Gramineae. It is a tropical African crop that was initially domesticated in India approximately 3000 years ago. It is predominantly cultivated in India as well as in various south-east Asian countries. Recent years have witnessed a resurgence of interest in kodo millet breeding, particularly owing to its outstanding nutritional profile. Kodo millet's ability to adapt to different marginal environments makes it promising to be grown as a part of sustainable agriculture. Availability of a plethora of diverse genetic resources in kodo millet has been instrumental in development of various improved cultivars through conventional breeding. Additionally, functional genomics has been instrumental in decoding the complex genetic architecture of kodo millet, thus enabling identification of key genes associated with drought tolerance, disease resistance, and improved nutritional profiling. Additionally, transcriptomics has deepened the insights into gene expression pattern in response to various stresses, offering valuable information for developing resistant genotypes. The expressed sequence tags (ESTs) available will surely benefit the scientists working on molecular breeding of millets through development and use of SSRs and SNPs markers under the marker assisted selection (MAS) scheme. This article examines potential directions for future research, including the advancement of genomics and targeted breeding approaches for holistic development of the kodo millet.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"15"},"PeriodicalIF":3.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142829804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets.

IF 3.6 3区生物学

Planta Pub Date : 2024-12-13 DOI: 10.1007/s00425-024-04593-x

Mauro Gismondi, Laura Strologo, Julieta Gabilondo, Claudio Budde, María Fabiana Drincovich, Claudia Bustamante

{"title":"Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets.","authors":"Mauro Gismondi, Laura Strologo, Julieta Gabilondo, Claudio Budde, María Fabiana Drincovich, Claudia Bustamante","doi":"10.1007/s00425-024-04593-x","DOIUrl":"https://doi.org/10.1007/s00425-024-04593-x","url":null,"abstract":"Main conclusion: PpZAT12, a transcription factor differentially expressed in peach varieties with distinct susceptibility tochilling injury (CI), is a potential candidate gene for CI tolerance by regulating several identified gene targets. ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a heat treatment applied before postharvest cold storage for reducing chilling injury (CI) symptoms. Here, the expression of PpZAT12 in different tissues and fruits subjected to either postharvest heat or cold treatments, was evaluated in peach varieties with differential susceptibility to develop CI. PpZAT12 increased by cold storage in CI-resistant cultivars ('Elegant Lady' and 'Rojo 2'), while it was not modified in a cultivar susceptible to develop CI ('Flordaking'). Besides, we expressed PpZAT12 in Arabidopsis (35S::PpZAT12) and found that these plants show impaired plant growth and development, rendering small plants with senescence delay and aborted seeds. We applied a proteomic approach to decipher the peptides responding to PpZAT12 in Arabidopsis and found 348 differential expressed proteins (DEPs) relative to the wild type. Besides, comparing the DEPs between Arabidopsis plants expressing PpZAT12 or AtZAT12 (35S::AtZAT12) we found common and specific responses to these TFs. Based on the proteomic information obtained here and published data about AtZAT12, we searched ZAT12-targets in peach allowing the identification of a putative ZAT12 regulon in this species. The identified peach ZAT12-protein targets could underlie the differential susceptibility to CI among different peach varieties and can be used as future targets to improve adaptation to refrigeration in fleshy fruits.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"14"},"PeriodicalIF":3.6,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ordered rise and disordered fall: dynamic changes of membrane lipids during girdling-induced tree mortality in Populus yunnanensis.

IF 3.6 3区生物学

Planta Pub Date : 2024-12-12 DOI: 10.1007/s00425-024-04582-0

Bo Gao, Faqing Tao, Mulan Wang, Xing Huang, Yuanxue Lu, Yanxia Jia, Xudong Zhang, Weiqi Li

{"title":"Ordered rise and disordered fall: dynamic changes of membrane lipids during girdling-induced tree mortality in Populus yunnanensis.","authors":"Bo Gao, Faqing Tao, Mulan Wang, Xing Huang, Yuanxue Lu, Yanxia Jia, Xudong Zhang, Weiqi Li","doi":"10.1007/s00425-024-04582-0","DOIUrl":"https://doi.org/10.1007/s00425-024-04582-0","url":null,"abstract":"Understanding the mechanisms behind drought-induced tree mortality is crucial for predicting the impact of global climate change on forests. We studied the mechanism at the cellular level in Populus yunnanensis by profiling membrane lipid molecules in leaves, branch phloem, top and bottom trunk phloem under trunk-girdling-induced drought conditions. We found that both lipid composition and content changed, depending on the tree's tissue positions and the progression of the girdling effect. The compositional changes were similar between the leaves and branches and between the top and bottom trunk phloem. The lipid content initially increased and then decreased until complete degradation, with similar fold increases between leaves and branch phloem, and between top and bottom trunk phloem. However, the fold increase in the former two was significantly lower than that in the latter two. The lipid composition remained stable during the increase but changed during the decrease. The decrease in phloem lipids occurred later than in leaves and simultaneously across positions. Our findings provide novel insights into the mechanisms of water deficit and carbohydrate allocation in drought-induced tree mortality, and suggest that the onset of phloem lipid degradation could serve as a threshold for predicting tree mortality.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"13"},"PeriodicalIF":3.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering the key lncRNAs in regulating cadmium accumulation and translocation in sweet sorghum.

IF 3.6 3区生物学

Planta Pub Date : 2024-12-11 DOI: 10.1007/s00425-024-04589-7

Kangqi Lin, Ze Yi, Sulian Lv, Bo Zhang, Zijin Guo, Yinxin Li

{"title":"Uncovering the key lncRNAs in regulating cadmium accumulation and translocation in sweet sorghum.","authors":"Kangqi Lin, Ze Yi, Sulian Lv, Bo Zhang, Zijin Guo, Yinxin Li","doi":"10.1007/s00425-024-04589-7","DOIUrl":"https://doi.org/10.1007/s00425-024-04589-7","url":null,"abstract":"Main conclusion: 1988 lncRNAs were identified in sweet sorghum roots under cadmium treatment; lncRNA 15962 and lncRNA 11558 were validated to be the key lncRNAs involved in regulating cadmium accumulation and translocation. Cadmium (Cd) has become one of the most harmful and widespread pollutants with industry development. Sweet sorghum is an ideal plant for phytoremediation of Cd-contaminated soil. However, little is known about the regulatory role of long non-coding RNAs (lncRNAs) associated with Cd stress response in sweet sorghum. Here, lncRNA-seq was carried out in the roots of two contrasting sweet sorghum genotypes (high-Cd accumulation genotype 'H18', and low-Cd accumulation genotype 'L69'). A total of 1988 lncRNAs were characterized, including 52 and 69 differentially expressed lncRNAs in 'H18' and 'L69' in response to Cd stress, respectively. Furthermore, the trans- or cis-target genes of lncRNAs were investigated. Then, 65 lncRNAs were characterized as the probable target of 117 miRNAs and 1888 genes were identified as putative cis-target genes of Cd-responsive lncRNAs. The dual-luciferase reporter assay indicated lncRNA 15962 may serve as the endogenous target mimics of sbi-miR5565e, which targeted two genes (Sobic.005G212900 and Sobic.009G144700) involved in cell wall metabolism. Four cis-target genes including SbYS1 which encoding a Cd chelate transporter, were up-regulated by overexpression of their corresponding lncRNAs in sweet sorghum protoplasts, suggesting the positive regulatory role of lncRNAs to these cis-target genes. Moreover, the expression of SbYS1 decreased when lncRNA 11558 was inhibited by exogenous miRNA application in 'H18' seedlings, further demonstrating the positive regulatory role of lncRNA 11558 to SbYS1. Altogether, our findings shed light on the regulatory role of lncRNAs associated with Cd accumulation and translocation in sweet sorghum.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"12"},"PeriodicalIF":3.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phyto-nutraceutical promise of Brassica vegetables in post-genomic era: a comprehensive review.

IF 3.6 3区生物学

Planta Pub Date : 2024-12-10 DOI: 10.1007/s00425-024-04587-9

Saurabh Singh, Anjan Das, Rajender Singh, Hela Chikh-Rouhou, Srija Priyadarsini, Alok Nandi

{"title":"Phyto-nutraceutical promise of Brassica vegetables in post-genomic era: a comprehensive review.","authors":"Saurabh Singh, Anjan Das, Rajender Singh, Hela Chikh-Rouhou, Srija Priyadarsini, Alok Nandi","doi":"10.1007/s00425-024-04587-9","DOIUrl":"10.1007/s00425-024-04587-9","url":null,"abstract":"Main conclusion: Brassica vegetables are one of the possible solutions to tackle the emerging human diseases and malnutrition due to their rich content of phyto-nutraceutaical compounds. The genomics enabled tools have facilitated the elucidation of molecular regulation, mapping of genes/QTLs governing nutraceutical compounds, and development of nutrient-rich Brassica vegetables. The enriched food products or foods as whole termed as functional foods are intended to provide health benefits. The 2500 year old Hippocratic phrase 'let thy food be thy medicine and thy medicine be thy food' remained in anonymity due to lack of sufficient evidence. However, today, we are facing reappraisal of healthy nutritious functional foods in battling diseases. In this context, the Brassica vegetables represent the most extensively investigated class of functional foods. An optimal consumption of Brassica vegetables is associated with lowering the risks of several types of cancer, chronic diseases, cardiovascular disease, and help in autism. In the post-genomic era, the integration of genetic and neoteric omics tools like transcriptomics, metabolomics, and proteomics have illuminated the downstream genetic mechanisms governing functional food value of Brassica vegetables. In this review, we have summarized in brief the phyto-nutraceutical profile and their functionality in Brassica vegetables. This review also highlights the progress made in identification of candidate genes/QTLs for accumulation of bioactive compounds in Brassica vegetables. We summarize the molecular regulation of major phytochemicals and breeding triumphs in delivering multifunctional Brassica vegetables.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"10"},"PeriodicalIF":3.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the mechanism of auxin-mediated fulvic acid-regulated root growth in Oryza sativa through physiological and transcriptomic analyses.

IF 3.6 3区生物学

Planta Pub Date : 2024-12-10 DOI: 10.1007/s00425-024-04573-1

Yi Tang, Ke Chen, Yanan Guo, Tianrui Li, Na Kuang, Zhixuan Liu, Haona Yang

{"title":"Investigating the mechanism of auxin-mediated fulvic acid-regulated root growth in Oryza sativa through physiological and transcriptomic analyses.","authors":"Yi Tang, Ke Chen, Yanan Guo, Tianrui Li, Na Kuang, Zhixuan Liu, Haona Yang","doi":"10.1007/s00425-024-04573-1","DOIUrl":"https://doi.org/10.1007/s00425-024-04573-1","url":null,"abstract":"As rice is one of the most crucial staple food sources worldwide, enhancing rice yield is paramount for ensuring global food security. Fulvic acid (FA), serving as a plant growth promoter and organic fertilizer, holds significant practical importance in studying its impact on rice root growth for improving rice yield and quality. This study investigated the effects of different concentrations of FA on the growth of rice seedlings. The results indicated that 0.05 g/L FA could promote the growth of rice seedlings, while 0.5 g/L FA inhibited root growth, reduced cell activity and enzyme activity in the root tips, and accumulated reactive oxygen species in root cells. To further elucidate the molecular mechanisms underlying these effects, we performed transcriptomic analysis and found that auxin (Aux) may be involved in the growth process mediated by FA. Furthermore, transcriptome heatmap analysis revealed a significant upregulation of the Aux/indoleacetic acid (Aux/IAA) gene family after FA treatment, suggesting that this gene family plays a crucial role in the impact of FA on root growth. Additionally, by detecting endogenous Aux content and adding exogenous Aux inhibitors, we confirmed the involvement of FA in rice seedling root growth as well as in the synthesis and transduction pathway of Aux.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"9"},"PeriodicalIF":3.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Functional characterization of two myo-inositol-1-phosphate synthase (MIPS) gene promoters from the halophytic wild rice (Porteresia coarctata).

IF 3.6 3区生物学

Planta Pub Date : 2024-12-10 DOI: 10.1007/s00425-024-04590-0

Papri Basak, Shiny Sangma, Abhishek Mukherjee, Tanushree Agarwal, Sonali Sengupta, Sudipta Ray, Arun Lahiri Majumder

引用次数: 0

Plant growth-promoting Bacillus amyloliquefaciens orchestrate homeostasis under nutrient deficiency exacerbated drought and salinity stress in Oryza sativa L. seedlings.

IF 3.6 3区生物学

Planta Pub Date : 2024-12-06 DOI: 10.1007/s00425-024-04585-x

Nikita Bisht, Tanya Singh, Mohd Mogees Ansari, Harshita Joshi, Shahank Kumar Mishra, Puneet Singh Chauhan

{"title":"Plant growth-promoting Bacillus amyloliquefaciens orchestrate homeostasis under nutrient deficiency exacerbated drought and salinity stress in Oryza sativa L. seedlings.","authors":"Nikita Bisht, Tanya Singh, Mohd Mogees Ansari, Harshita Joshi, Shahank Kumar Mishra, Puneet Singh Chauhan","doi":"10.1007/s00425-024-04585-x","DOIUrl":"https://doi.org/10.1007/s00425-024-04585-x","url":null,"abstract":"Main conclusion: Nutrient deficiency intensifies drought and salinity stress on rice growth. Bacillus amyloliquefaciens inoculation provides resilience through modulation in metabolic and gene regulation to enhance growth, nutrient uptake, and stress tolerance. Soil nutrient deficiencies amplify the detrimental effects of abiotic stresses, such as drought and salinity, creating substantial challenges for overall plant health and crop productivity. Traditional methods for developing stress-resistant varieties are often slow and labor-intensive. Previously, we demonstrated that plant growth-promoting rhizobacteria Bacillus amyloliquefaciens strain SN13 effectively alleviates stress induced by sub-optimum nutrient conditions in rice. In this study, we evaluated the effectiveness of SN13 in reducing the compounded impacts of drought and salinity under varying nutrient regimes in rice seedlings. The results demonstrated that PGPR inoculation not only improved the growth parameters, nutrient content, and physio-biochemical characteristics under nutrient-limited conditions, but also reduced the oxidative stress markers. The altered expression of stress-related and transcription factor genes (USP, DEF, CYP450, GST, MYB, and bZIP) revealed the regulatory effect of PGPR in enhancing stress tolerance through these genes. GC-MS-based untargeted metabolomic analysis revealed that PGPR significantly influenced various metabolic pathways, including galactose metabolism, fructose and mannose metabolism, and fatty acid biosynthesis pathways, suggesting that PGPR affects both energy production and stress-protective mechanisms, facilitating better growth and survival of rice seedlings.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"8"},"PeriodicalIF":3.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing CaPIP5K4-1 leads to decreased male fertility in Capsicum annuum L.

IF 3.6 3区生物学

Planta Pub Date : 2024-12-04 DOI: 10.1007/s00425-024-04584-y

Weifu Kong, Panpan Duan, Yuhang Wang, Tao Zhang, Mianzhu Huang, Jingtao Kang, Lina Wang, Bingqiang Wei, Yajun Chang

{"title":"Silencing CaPIP5K4-1 leads to decreased male fertility in Capsicum annuum L.","authors":"Weifu Kong, Panpan Duan, Yuhang Wang, Tao Zhang, Mianzhu Huang, Jingtao Kang, Lina Wang, Bingqiang Wei, Yajun Chang","doi":"10.1007/s00425-024-04584-y","DOIUrl":"https://doi.org/10.1007/s00425-024-04584-y","url":null,"abstract":"Main conclusion: Phosphatidylinositol 4-phosphate 5-kinase gene CaPIP5K4-1 is highly expressed in the pepper anthers. Virus-induced gene silencing of CaPIP5K4-1 leads to reduced male fertility in pepper. The phosphatidylinositol 4-phosphate 5-kinase (PIP5K) is a pivotal enzyme in the phosphatidylinositol signaling pathway, and its crucial involvement in both plant development and stress response has been established. Here, we found that the expression of CaPIP5K4-1 in pepper was significantly higher in the fertile flower buds compared to sterile flower buds. Furthermore, its expression was validated in anthers and pollens by qRT-PCR and RNA-ISH assays, respectively. Its GFP fusion protein was mainly located on the plasma membrane. Silencing CaPIP5K4-1 in fertile pepper accessions resulted in wrinkled pollen grain cell walls, decreased pollen germination efficiency, and inhibited pollen tube growth. The transcription levels of multiple genes in the phosphatidylinositol signaling pathway were also assessed. Five phospholipase C (PLC) genes were downregulated in silenced plants. On the contrary, inositol phosphatase SAC and phosphatase and tensin homolog (PTEN) were upregulated. This study reported the role of CaPIP5K4-1 in pepper male fertility and provided insights into the regulatory mechanisms of PI signaling in pepper.","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 1","pages":"7"},"PeriodicalIF":3.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0