Planta最新文献

{"title":"Trends in plant tissue culture, production, and secondary metabolites enhancement of medicinal plants: a case study of thyme.","authors":"Aicha Nordine","doi":"10.1007/s00425-025-04655-8","DOIUrl":"https://doi.org/10.1007/s00425-025-04655-8","url":null,"abstract":"<p><strong>Main conclusion: </strong>Thymus plants are greatly threatened by overharvesting and climate change. Plant cell and tissue culture techniques provide effective alternatives for the production and the enhancement of both biomass and bioactive compounds. Medicinal and aromatic plants are rich sources of various bioactive compounds known as secondary metabolites, which are used across a range of fields, including medicinal, cosmetics, pharmaceuticals, perfumes, agrochemicals and agrofood industries. Thyme is considered one of the most popular herbs globally, valued for its significant medicinal, pharmaceutical, and nutritional benefits. However, its natural habitats are rapidly diminishing due to excessive harvesting and climate change. Consequently, several approaches have been developed to find alternatives to harvesting wild thyme. Plant cell and tissue culture techniques offer a superior alternative to traditional propagation methods, such as seeds, cuttings, or tuft division. These techniques enable the production of large quantities of uniform, disease-free plantlets for commercial cultivation and facilitate the development of new genotypes. Additionally, they support the production and enhancement of bioactive compounds from thyme plants. This review explores the application of plant cell, tissue, and organ culture biotechnology in thyme plants, focusing on enhancing production and improving secondary metabolite yields and biomass production.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"84"},"PeriodicalIF":3.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional reprogramming and microbiome dynamics in garden pea exposed to high pH stress during vegetative stage.","authors":"Asha Thapa, Md Rokibul Hasan, Ahmad H Kabir","doi":"10.1007/s00425-025-04656-7","DOIUrl":"https://doi.org/10.1007/s00425-025-04656-7","url":null,"abstract":"<p><strong>Main conclusion: </strong>High soil pH induces the upregulation of genes involved in oxidative stress and nutrient transport, while the enrichment of beneficial microbes (Variovorax, Chaetomium, and Pseudomonas) highlights their potential role in promoting stress adaptation. High soil pH severely impacts plant growth and productivity, yet the transcriptomic changes and microbial dynamics underlying stress adaptation in garden pea (Pisum sativum ssp. hortense) remain unclear. This study demonstrates that high soil pH leads to stunted growth, reduced biomass, impaired photosynthesis, and nutrient status in garden pea. Further, disruption in key nitrogen-fixing bacteria (Rhizobium indicum, R. leguminosarum, and R. redzepovicii), along with the downregulation of NifA and NifD genes and upregulation of NifH in nodules highlights the critical role of micronutrient balance in legume-microbe symbiosis and a compensatory response to maintain nitrogen status. RNA seq analysis revealed extensive transcriptional reprogramming in roots, characterized by the upregulation of oxidative stress response genes (e.g., oxidoreductase and glutathione transferase activities, metal ion transporters) and the downregulation of genes related to ammonia-lyase activity and ion binding, reflecting broader disruptions in nutrient homeostasis. KEGG pathway analysis identified enrichment of MAPK signaling pathway, likely interacting with other pathways associated with stress tolerance, metabolic adjustment, and structural reorganization as part of adaptive responses to high pH. Root microbiome analysis showed significant enrichment of Variovorax, Shinella, and Chaetomium, suggesting host-driven recruitment under high pH stress. Stable genera, such as Pseudomonas, Novosphingobium, Mycobacterium, Herbaspirillum, and Paecilomyces, displayed resilience to stress conditions, potentially forming core microbiome components for adaptation to high pH. In a targeted study, inoculation of plants with an enriched microbiome, particularly C. globosum, under high pH conditions improved growth parameters and increased the abundance of Stenotrophomonas and Pseudomonas in the roots. It suggests that these bacterial genera may act as helper microbes to C. globosum, collectively promoting stress resilience in pea plants suffering from high pH. These findings provide a foundation for microbiome-aided breeding programs and the development of microbial consortia to enhance the adaptation of pea plants to high pH conditions.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"83"},"PeriodicalIF":3.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multidisciplinary and integrative review of the structural genome and epigenome of Capsicum L. species.","authors":"Breno Machado de Almeida, Wellington Ronildo Clarindo","doi":"10.1007/s00425-025-04653-w","DOIUrl":"https://doi.org/10.1007/s00425-025-04653-w","url":null,"abstract":"<p><strong>Main conclusion: </strong>We revised and integrated the genomic and epigenomic data into a comparative Capsicum ideogram, evidencing the advances and future perspectives. Capsicum L. (Solanaceae) genome has been characterized concerning karyotype, nuclear and chromosomal genome size, genome sequencing and physical mapping. In addition, the epigenome has been investigated, showing chromosomal distribution of epimarks in histone amino acids. Genetic and epigenetic discoveries have given light to understanding the structure and organization of the Capsicum \"omics\". In addition, interspecific and intraspecific similarities and diversities have been identified, characterized and compared in taxonomic and evolutive scenarios. The journey through Capsicum studies allows us to know the 2n = 2x = 24 and 2n = 2x = 26 chromosome numbers, as well as the relatively homomorphic karyotype, and the 1C chromosomal DNA content. In addition, Capsicum \"omics\" diversity has mainly been evidenced from the nuclear 1C value, as well as from repeatome composition and mapping. Like this, Capsicum provides several opportunities for \"omics\", ecological, agronomic and conservation approaches, as well as subjects that can be used at different levels of education. In this context, we revisit and integrate Capsicum data about the genome size, karyotype, sequencing and cytogenomics, pointing out the progress and impact of this knowledge in taxonomic, evolutive and agronomic contexts. We also noticed gaps, which can be a focus of further studies. From this multidisciplinary and integrative review, we intend to show the beauty and intrigue of the Capsicum genome and epigenome, as well as the outcomes of these similarities and differences.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"82"},"PeriodicalIF":3.6,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of copy number variation in diverse black pepper accessions.","authors":"Parinita Das, T E Sheeja, Bibek Saha, A Fayad, Tilak Chandra, U B Angadi, M S Shivakumar, T P Muhammed Azharudheen, Sarika Jaiswal, Mir Asif Iquebal, Dinesh Kumar","doi":"10.1007/s00425-025-04658-5","DOIUrl":"https://doi.org/10.1007/s00425-025-04658-5","url":null,"abstract":"<p><strong>Main conclusion: </strong>A genome-wide resequencing analysis reveals the involvement of copy-number variation in black pepper accessions influencing advantageous agronomic traits and the evolution of elite genotypes with specific attributes. Black pepper (Piper nigrum L.) is renowned as a versatile seasoning, offers numerous health benefits, and possesses historical significance in the global spice trade. Variations in advantageous agronomic traits among diverse black pepper genotypes underscore the potential value of understanding their underlying genetic regulation. One emerging genetic component of interest is copy number variation (CNV), which represents a significant source of intra-varietal genetic diversity by altering the dosage of DNA segments, thereby influencing phenotypic characteristics. In this study, we conducted genome-wide resequencing of thirty-nine black pepper germplasm accessions to identify high-confidence CNVs and explore their functional impact on agronomic traits. Our analysis identified a total of 159,390 CNVs and delineated 11,360 CNV regions (CNVRs) with an average length of 87,106 base pairs. Among these, we observed 82,027 deletion and 77,363 duplication events across the 26 chromosomes of black pepper. Notably, deletions were more frequent than duplications. Enrichment analysis of genes harbored within CNVRs revealed their involvement primarily in piperine biosynthesis and immune response pathways. Principal component analysis demonstrated varietal differentiation and highlighted intra-varietal evolutionary linkage among tested genotypes, suggesting that CNVRs play a significant role in shaping the evolution of elite black pepper genotypes. For instance, genotype IISR-Malabar Excel exhibited the highest number of deletions, whereas genotype Acc:7211 showed the most duplications. Chromosome 1 exhibited the highest frequency of deletions, while chromosome 3 showed the highest frequency of duplications. While the overall number of CNVRs did not significantly differ among genotypes, 33 CNVRs contained genes crucial for phenylpropanoid biosynthesis, with 14 genes undergoing deletions and 19 genes showing duplications. Additionally, we developed a web resource BPCNVDb, which could be retrieved ( https://bpcnvdb.daasbioinfromaticsteam.in/index.php ) to facilitate access to genotype-specific CNVs and CNVRs, aiming to enhance breeding efforts in black pepper.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"81"},"PeriodicalIF":3.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of a key gene controlling purple leaf coloration in non-heading Chinese cabbage (Brassica rapa).","authors":"Jia Si, Xiaoqing Zhou, Xinyu Chen, Huilin Ming, Hanqiang Liu, Maixia Hui","doi":"10.1007/s00425-025-04630-3","DOIUrl":"https://doi.org/10.1007/s00425-025-04630-3","url":null,"abstract":"<p><strong>Main conclusion: </strong>Chalcone isomerase (BraCHI, BraA03g059660.3C) is the candidate gene controlling purple leaf coloration in non-heading Chinese cabbage. A 10-bp deletion in its promoter enhances gene expression in purple plants, likely by disrupting MYB transcription factor binding, leading to anthocyanin accumulation. Leaf color is a critical trait influencing the commercial and nutritional value of leafy vegetables, with purple-leafed varieties prized for their high anthocyanin content. In this study, we investigated the genetic basis of purple leaf coloration in non-heading Chinese cabbage (Brassica rapa). Using a recombinant inbred line (RIL) population derived from a cross between purple-leafed S45P and green-leafed S45G lines, bulked segregant analysis sequencing (BSA-seq) and fine mapping were performed. The analysis identified BraP2, a locus on chromosome A03 associated with purple leaf coloration. Within the 65.31 kb candidate region, BraA03g059660.3C, encoding chalcone isomerase (CHI), was identified as the strongest candidate gene. Quantitative real-time PCR (qRT-PCR) revealed significantly higher expression of BraA03g059660.3C in purple-leafed S45P plants compared to green-leafed S45G plants. Further sequence analysis uncovered a 10-bp deletion in the promoter region of BraA03g059660.3C in S45P plants. This deletion likely disrupts a MYB transcription factor binding site, enhancing gene expression and promoting anthocyanin accumulation. Our findings demonstrate that BraA03g059660.3C plays a pivotal role in controlling purple leaf coloration in non-heading Chinese cabbage. This discovery advances the understanding of anthocyanin biosynthesis regulation and provides valuable genetic resources for breeding Brassica crops with improved esthetic and nutritional qualities.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"80"},"PeriodicalIF":3.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omics advancements towards exploring arsenic toxicity and tolerance in plants: a review.","authors":"Sayyeda Hira Hassan, Melissa Simiele, Gabriella Stefania Scippa, Domenico Morabito, Dalila Trupiano","doi":"10.1007/s00425-025-04646-9","DOIUrl":"10.1007/s00425-025-04646-9","url":null,"abstract":"<p><strong>Main conclusion: </strong>Omics approaches provide comprehensive insights into plant arsenic stress responses, setting the stage for engineering arsenic-tolerant crops. Understanding arsenic (As) toxicity in plants is crucial for environmental and agricultural sustainability, considering the implications of As in impacting soil productivity and environmental health. Although some articles already examined the detailed molecular mechanisms behind As toxicity and tolerance, a comprehensive review of recent omics advancements in studying plant responses to As exposure is needed. The present review highlights the valuable contribution of omics approaches (genomics, transcriptomics, proteomics, and metabolomics) to characterize the intricate response to As overall, which could empower As-tolerant plant development. Genomic techniques, such as QTL mapping, GWAS, RAPD, and SSH, hold the potential to provide valuable insights into the genetic diversity and expression patterns associated with the plant response to As stress, highlighting also the power of new advanced technology such as CRISPR-Cas9. Transcriptomics approaches (e.g., microarrays and RNA sequencing) revealed gene expression patterns in plants under As stress, emphasizing the role of sulfur metabolism in As tolerance. Proteomics, using 2-DE combined with MALDI-ToF MS or ESI-MS/MS, offers insights into the stress-inducible proteins and their involvement in As toxicity mitigation, while iTRAQ-based proteomics enabled an understanding of cultivar-specific responses under high As concentration. Metabolomics, with LC-MS, GC-MS, (U)HPLC, and NMR, elucidated small molecule alterations and complex metabolic activities occurring under As plant exposure. Compendium of data and evidence-related tools offers a foundation for advancing As-tolerant plant development and promoting environmental and agricultural resilience.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"79"},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge of microalgal Rubiscos helps to improve photosynthetic efficiency of crops.","authors":"Tongtong Zhu, Peng Ning, Yiguo Liu, Min Liu, Jianming Yang, Zhaobao Wang, Meijie Li","doi":"10.1007/s00425-025-04645-w","DOIUrl":"https://doi.org/10.1007/s00425-025-04645-w","url":null,"abstract":"<p><strong>Main conclusion: </strong>A comprehensive understanding of microalgal Rubiscos offers opportunities to enhance photosynthetic efficiency of crops. As food production fails to meet the needs of the expanding population, there is increasing concern about Ribulose-1, 5-diphosphate (RuBP) carboxylase/oxygenase (Rubisco), the enzyme that catalyzes CO₂ fixation in photosynthesis. There have been many attempts to optimize Rubisco in crops, but the complex multicellular structure of higher plants makes optimization more difficult. Microalgae have the characteristics of rapid growth, simple structure and easy molecular modification, and the function and properties of their Rubiscos are basically the same as those of higher plants. Research on microalgal Rubiscos helps to broaden the understanding of Rubiscos of higher plants. Also, transferring all or part of better microalgal Rubiscos into crop cells or giving crop Rubiscos the advantages of microalgal Rubiscos can help improve the photosynthesis of crops. In this review, the distribution, origin, evolution, molecular structure, folding, assembly, activation and kinetic properties of microalgal Rubiscos are summarized. Moreover, the development of some effective methods to improve the properties and application of Rubiscos in microalgae are also described.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"78"},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PpSPL1 and PpSPL15 inhibit peach branching by increasing strigolactone synthesis.","authors":"Wan Pei, Jie Zhang, Ruixian Shen, Hefang Xie, Yajia Zhang, Junjie Zhang, Xiaodong Lian, Haipeng Zhang, Nan Hou, Lei Wang, Xianbo Zheng, Jun Cheng, Wei Wang, Xia Ye, Jidong Li, Xiaobei Wang, Jiancan Feng, Bin Tan","doi":"10.1007/s00425-025-04659-4","DOIUrl":"https://doi.org/10.1007/s00425-025-04659-4","url":null,"abstract":"<p><strong>Main conclusion: </strong>PpSPL1 and PpSPL15 inhibit peach branching by directly binding to and upregulating the expression of strigolactone (SL) synthesis gene PpLBO1. Branch number is a crucial agronomic trait that influences tree architecture, directly affecting fruit yield and quality. It remains unknown whether SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL), an important transcription factor in determining plant architecture, is involved in the peach branching process. In this study, we found that PpSPL1 and PpSPL15 exhibited significantly higher expression levels in pillar type peach 'Sahonglongzhu' (with fewer secondary branches) compared to standard-type peach 'Okubo' (with more secondary branches). PpSPL1 and PpSPL15 can directly bind to the promoter of the SL synthesis gene PpLBO1. Transient overexpression of PpSPL1 and PpSPL15 in 'Sahonglongzhu' peach axillary buds significantly increased the expression of PpLBO1 and endogenous SL content. Conversely, opposite results were obtained when the expression of PpSPL1 and PpSPL15 was transiently silenced in peach axillary buds. Gene function analysis indicated that transient overexpression of PpSPL1 and PpSPL15 in peach seedlings clearly inhibited peach branching. On the contrary, the number of branches dramatically increased when the expression of PpSPL1 and PpSPL15 were transiently silenced in peach seedlings. These results suggested that PpSPL1 and PpSPL15 could bind to and enhance the expression of PpLBO1, further inhibiting peach branching.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"77"},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing sweet potato production: a comprehensive analysis of the role of auxins and cytokinins in micropropagation.","authors":"Gideon Adu Donyina, Adrienn Szarvas, Vincent Agyemang Opoku, Edit Miko, Melinda Tar, Szilárd Czóbel, Tamás Monostori","doi":"10.1007/s00425-025-04650-z","DOIUrl":"10.1007/s00425-025-04650-z","url":null,"abstract":"<p><strong>Main conclusion: </strong>This review emphasizes the prevalent auxins and cytokinins used in sweet potato micropropagation, their optimal concentrations for effective in vitro regeneration, various propagation techniques, and Africa's potential to improve sweet potato production. Ipomoea batatas (L.) Lam., or sweet potato, is a robust, nutritious, and adaptable crop traditionally propagated through conventional methods. These techniques, however, have limitations, prompting the adoption of micropropagation as an efficient alternative for producing healthy, cost-effective plantlets in reduced time. This review critically evaluates the influence of auxins and cytokinins, the most frequently utilized plant growth regulators (PGRs), in enhancing sweet potato micropropagation protocols. The study examines the crop's origins, distribution, and cultivation practices, as well as the morphophysiological effects of PGRs on sweet potatoes. Our analysis reveals that 6-benzylaminopurine (BAP) and N6-benzyladenine (BA) are the predominant cytokinins, while naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) are the primary auxins employed in sweet potato micropropagation. The review also proposes strategies for increasing production, particularly in Africa, and identifies areas requiring further investigation to better understand how these growth regulators impact the physiological development and response of sweet potatoes to environmental stress. This comprehensive assessment contributes to the expanding knowledge base on sweet potato micropropagation and offers valuable insights for researchers and practitioners in the field.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"74"},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interacting effects of crop domestication and soil resources on leaf and root functional traits.","authors":"Victoria Nimmo, Gurcharn S Brar, Adam R Martin, Marney E Isaac","doi":"10.1007/s00425-025-04635-y","DOIUrl":"https://doi.org/10.1007/s00425-025-04635-y","url":null,"abstract":"<p><strong>Main conclusion: </strong>Domestication altered wheat leaf functional trait expression, and soil amendments altered root trait expression. These alterations shape crop suitability to stressed environments, and informs variety selection for agronomic conditions. Crop traits have been altered through domestication, resulting in syndromes that assist modern crops in contending with environmental constraints. Yet, we have limited understanding of how domestication has shaped the ability of crops to alter leaf and root functional traits for optimal performance under contemporary agronomic conditions, such as water limitation and organic amendments. We used a greenhouse pot experiment that included a wild progenitor of wheat (Aegilops tauschii), three domesticated wheat (Triticum aestivum) varieties (Watkins, Red Fife and Marquis), and three modern wheat varieties (developed from 1969 to 2016) to assess the effects of domestication on crop functional traits under water limitation and under organic and inorganic soil amendments, and to evaluate how this trait expression moderates rhizosphere soil conditions. Leaf functional trait expression varied significantly across wheat domestication classes, with these differences being almost independent of soil amendment or watering treatments. The wild progenitor expressed resource conservative leaf trait values, with low water use efficiency and stomatal conductance. Root trait expression was influenced by both soil amendment and watering treatment, with all wheat lineages expressing acquisitive traits, e.g., higher specific root length and lower root diameter, under organic amendments. Soil amendments and watering treatments impacted rhizosphere conditions, including microbial diversity and acid phosphatase activity, and domestication class impacted fungal diversity. Broadly, domestication altered the expression of wheat leaf functional traits, and soil amendments altered the expression of wheat root functional traits. These alterations in trait expression and rhizosphere soil response shape crop suitability to drought-prone or nutrient stressed environments, and should be considered when selecting varieties for hybridization for contemporary agronomic conditions.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"261 4","pages":"75"},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}