Zewei Wang, Zimu Yu, He Zhu, Xixi Jing, Dingxin Liu, Hao Zhang, Xin Yang, Renwu Zhou, Mingzhe Rong
{"title":"Plasma‐Activated Saline Resensitizes Cisplatin‐Resistant T24 Bladder Cancer Cells","authors":"Zewei Wang, Zimu Yu, He Zhu, Xixi Jing, Dingxin Liu, Hao Zhang, Xin Yang, Renwu Zhou, Mingzhe Rong","doi":"10.1002/ppap.70127","DOIUrl":"https://doi.org/10.1002/ppap.70127","url":null,"abstract":"ABSTRACT Plasma‐activated saline (PAS) loads reactive oxygen and nitrogen species, triggering oxidative stress and tumor death. However, PAS exhibits poor anticancer efficacy due to rapid dilution and reactive species decay. Herein, we developed a plasma bubble reactor to activate saline and investigate its synergy with cisplatin (DDP) against DDP‐resistant T24 bladder cancer cells (T24/DDP) in vitro. The results showed that PAS‐DDP significantly reduced the cell viability of T24/DDP to 32.4%, surpassing PAS (52.9%) or DDP alone (96.9%). PAS‐DDP significantly inhibited T24/DDP cells by intracellular reactive oxygen species accumulation, mitochondrial depolarization, and cell apoptosis induction. Moreover, PAS‐DDP suppressed the p62/Keap1/Nrf2 signaling pathway, restoring oxidative sensitivity and boosting DDP efficacy. These findings suggest that PAS could enhance chemotherapy efficacy and overcome drug resistance.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ppap.70127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147911534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoran Wang, Mengying Zhu, LongFei Hong, Shuai Yuan, Chenyu Liu, Zhuo Li, Behdad Soltani, Tianqi Zhang, Dehui Xu, A. Ya. Nikiforov, Anna Dzimitrowicz, Piotr Jamróz, Ruonan Ma, Dingxin Liu, Renwu Zhou, Patrick J. Cullen
{"title":"Microbubble‐Enhanced Plasma for Water Disinfection: Improved Reactive Species Yield and Synergistic Sterilization Mechanism","authors":"Xiaoran Wang, Mengying Zhu, LongFei Hong, Shuai Yuan, Chenyu Liu, Zhuo Li, Behdad Soltani, Tianqi Zhang, Dehui Xu, A. Ya. Nikiforov, Anna Dzimitrowicz, Piotr Jamróz, Ruonan Ma, Dingxin Liu, Renwu Zhou, Patrick J. Cullen","doi":"10.1002/ppap.70126","DOIUrl":"https://doi.org/10.1002/ppap.70126","url":null,"abstract":"ABSTRACT Plasma‐activated water (PAW) offers a promising alternative to conventional disinfection methods in biomedical applications. However, most existing PAW preparation methods are constrained by mass transfer limitations of reactive species, reducing overall effectiveness. To address this, a Venturi tube‐based microbubble generator is integrated with a coaxial dielectric barrier discharge system. The combined system generates tunable microbubbles (< 200 µm) and achieves a 13.8‐fold enhancement in ozone dissolution versus plasma alone. Both reactive oxygen and nitrogen species are detected, with concentrations varying with gas flow rates. This plasma‐micobubble system achieves a 4.79‐log (99.99%) inactivation of Staphylococcus aureus within 5 min, with ONOO⁻ and O 2 ⁻ identified as dominant reactive species. This study presents a scalable and effective plasma‐microbubble technology for safeguarding water quality.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Plasma‐Activated Saline Triggers Macrophage M1 Polarization by Inducing Tumor Cell Apoptosis","authors":"Kejun Qu, Yifan Zhang, Chan Qiang, Zimu Yu, Xixi Jing, Zewei Wang, Jishen Zhang, Zifeng Wang, Dingxin Liu, Hao Zhang, Xin Yang, Mingzhe Rong","doi":"10.1002/ppap.70132","DOIUrl":"https://doi.org/10.1002/ppap.70132","url":null,"abstract":"ABSTRACT Here, we investigated the effects of aqueous reactive oxygen species (ROS) in surface air plasma‐activated solution (PAS) on the bladder cancer cell line MB49 and their role in remodeling the polarization status of tumor‐associated macrophages (TAMs). The study reveals substantial ROS production by plasma within PAS. In vitro experiments showed that the PAS exhibited an activation time‐dependent and significant cytotoxic effect on MB49 cells. Further validation using a transwell coculture model demonstrated that pretreatment of MB49 cells with PAS promoted the polarization of RAW264.7 macrophages toward the antitumor M1 phenotype. Our study reveals the dual role of PAS in direct tumor killing and immune regulation.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ppap.70132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147906809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zizhu Zhang, J. Zhang, Mengke Li, Qiuyi Yue, Jing Shu, Pengfei Zhang, Zihao Ye, Jinkun Chen, Zifeng Wang, Hao Zhang, Li Guo, Dingxin Liu, Mingzhe Rong
{"title":"Hydrogen Peroxide Enhances the Therapeutic Efficacy of Plasma‐Activated Gas for Infected Wound Treatment","authors":"Zizhu Zhang, J. Zhang, Mengke Li, Qiuyi Yue, Jing Shu, Pengfei Zhang, Zihao Ye, Jinkun Chen, Zifeng Wang, Hao Zhang, Li Guo, Dingxin Liu, Mingzhe Rong","doi":"10.1002/ppap.70117","DOIUrl":"https://doi.org/10.1002/ppap.70117","url":null,"abstract":"ABSTRACT Plasma‐activated gas (PAG) offers advantages over direct plasma for wound treatment but suffers efficacy loss due to reactive species quenching by wound exudate proteins. To overcome this, we propose combining PAG with hydrogen peroxide (H 2 O 2 ). In vitro sterilization experiments revealed that co‐treatment with 1% H 2 O 2 and PAG on Staphylococcus aureus ( S. aureus ) suspensions containing bovine serum albumin (BSA) achieved a bacterial reduction exceeding six orders of magnitude, enhancing generation of H 2 O 2 , NO 3 − , and ONOO − . Animal experiments demonstrated that in infected wounds, PAG‐H 2 O 2 co‐treatment significantly reduced bacterial load. Immunohistochemical and biochemical analyses showed this combination promoted healing by suppressing inflammation and inducing growth factors, without systemic toxicity. This novel approach markedly amplifies the therapeutic efficacy of PAG in treating infected wounds.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147894882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dielectric Barrier Discharge Plasma Coupled With Microbubbles for the Degradation of Tetracycline in Wastewater","authors":"Wei Wei, Ziyan Ni, Yuxiang Dong, Zhenghan Zhou, Yuhao Ge, Jiayao Li, Bangwu Deng, Xiachun Wu, Xianhuai Huang, Shuguang Zhu, BeiPing Zhang","doi":"10.1002/ppap.70112","DOIUrl":"https://doi.org/10.1002/ppap.70112","url":null,"abstract":"ABSTRACT Dielectric barrier discharge (DBD) can remove antibiotics from wastewater, but has challenges like high energy consumption and industrialization difficulties. This study coupled DBD with microbubbles (MBs) to investigate its degradation effect on tetracycline (TC). Experimental results showed that introducing MBs and optimizing via response surface methodology achieved optimal degradation and energy efficiency rates of 98.31% and 3.49 g·kW −1 ·h −1 , respectively. More reactive oxygen species were generated in the DBD‐MB system. Superoxide anion radicals (O 2 •− ), hydroxyl radicals (•OH), and singlet oxygen ( 1 O 2 ) were key to enhancing TC degradation. A potential degradation pathway for TC was proposed. This work combined MBs and DBD to rapidly degrade TC, providing a novel strategy for antibiotic wastewater treatment and contributing to technological advancement.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ppap.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147883029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fabrication and Analysis of Anticancer Effects of Plasma‐Activated Saline: Spatiotemporal Evolution of SDBD Plasma and Feedback of the Liquid Temperature","authors":"Yujing Xu, Jinkun Chen, Hao Zhang, Xixi Jing, Zewei Wang, Jishen Zhang, Zifeng Wang, Dingxin Liu, Jianbao Zheng, Jingyao Zhang, Mingzhe Rong, Paul K. Chu","doi":"10.1002/ppap.70099","DOIUrl":"https://doi.org/10.1002/ppap.70099","url":null,"abstract":"ABSTRACT Surface dielectric barrier discharge (SDBD) plasma ionizes ambient air at room temperature to generate reactive oxygen and nitrogen species (RONS). Gaseous RONS transferred into saline yield plasma‐activated saline (PAS) showing promise for intracavitary tumor therapy. Hereon, the discharge parameters, RONS generation, and therapeutic efficacy on colorectal tumors using PAS are investigated. Elevated electrode temperatures increase discharge channels and ionization. Higher gas temperature and humidity facilitate NO₂, N₂O, and nitrogen oxides formation. PAS prepared with 23°C saline (vs. 4°C) enhances water evaporation and RONS loading, improving anticancer effects via metabolic disruption in vitro. In vivo, PAS immersion suppresses tumor growth without systemic toxicity. Initial solution parameters critically modulate SDBD discharge behavior and RONS generation, which are essential for optimizing therapeutic PAS.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"23 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ppap.70099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of Plasma‐Activated Mist for the Control of Plant Insect Pests","authors":"Pengyu Zhao, Rui Zhang, Sihong Ma, Tian-Yi Song, Zhenyu Wang, Feng Zhang, Jamoliddin Razzokov, Nodira Azimova, Zifeng Wang, Hao Zhang, Li Guo, Dingxin Liu, Xiaohua Wang, Mingzhe Rong","doi":"10.1002/ppap.70091","DOIUrl":"https://doi.org/10.1002/ppap.70091","url":null,"abstract":"ABSTRACT Plant pests pose a significant threat to agricultural productivity, while chemical pesticides cause environmental concerns. In this study, plasma‐activated mist (PAM) generated by mixing water mist with plasma‐activated gas could effectively inactivate Myzus persicae adults and Helicoverpa armigera larvae and eggs. PAM treatment induced an increase in oxidative stress markers, such as malondialdehyde levels and superoxide dismutase activities in pests. The reactive species in PAM left only trace nitrate residues on leaves. Furthermore, the 50‐day consecutive PAM treatment had little effects on the growth and appearance of the Malabar spinach. Therefore, the PAM supplied a promising, effective, and environmentally‐friendly alternative for large‐scale applications in agricultural fields.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D Zhang, Zewei Wang, Zhixiang Yu, Xixi Jing, Xinwei Lu, Hao Zhang, Jishen Zhang, Dingxin Liu, Cheng Wang, Mingzhe Rong
{"title":"Air Discharge Plasma‐Activated Hydrogels Combined With Nitrogenous Nanographene Oxide Exhibit Enhanced Anticancer Efficacy","authors":"D Zhang, Zewei Wang, Zhixiang Yu, Xixi Jing, Xinwei Lu, Hao Zhang, Jishen Zhang, Dingxin Liu, Cheng Wang, Mingzhe Rong","doi":"10.1002/ppap.70080","DOIUrl":"https://doi.org/10.1002/ppap.70080","url":null,"abstract":"ABSTRACT Cold atmospheric plasma‐activated hydrogel (PAH) have proven effective as a novel anticancer method. In this study, air discharge plasma‐activated pluronic F127 hydrogel (PAH PF127 ) combined with nitrogenous nanographene oxide (GO/N) are proposed for enhancing anticancer efficacy. The results showed that PAH PF127 could effectively load various reactive oxygen and nitrogen species generated by plasma without adversely affecting the physicochemical properties. In addition, the in vitro results indicated that the PAH PF127 had an anticancer effect on B16F10 melanoma cells, and the combination treatment reduced cancer cell viability to 49%, compared to 73% for PAH PF127 and 88% for GO/N alone, and increased intracellular ROS levels by 2.4‐fold. These findings highlight the potential of PAH PF127 ‐GO/N as an innovative platform for enhanced plasma‐induced cancer therapy.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"22 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Discharge Dynamics and Remote Ionization Wave Modulation in Endoscopic Plasma Jet","authors":"Runze Liang, Chenguo Yao, Kai Chen, Feiyu Wu, Jialei Wang, Ting Zhu, Qiang Yang, Hongyu Kou, Shoulong Dong","doi":"10.1002/ppap.70054","DOIUrl":"https://doi.org/10.1002/ppap.70054","url":null,"abstract":"ABSTRACT Plasma delivery through endoscopic systems enables remote plasma applications but is constrained by limited understanding of discharge dynamics. We develop a self‐consistent plasma‐fluid model coupled with dynamic Drude formulation to capture the coupling between metallic electrode and ionization wave during plasma discharge. Simulations reveal an asynchronous two‐stage discharge: an internal ionization wave (IIW) forms a conductive channel, followed by a tip ionization wave (TIW) at the electrode apex. The coupling mechanism between IIW and TIW through the conductive channel is identified, achieving remote plasma generation and modulation in a bronchial phantom. These insights establish a framework for spatially controlled plasma production and provide a foundation for advancing plasma‐based applications, including Interventional tumor ablation, Pipe sterilization, and targeted surface modification.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"22 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147902847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chenglong Ding, Zhidan Sun, Y. Andrew Wang, Han Zhou, Qing Huang, Wencheng Song
{"title":"Effect of Flexible Plasma Pad on HaCaT Cells and Bacteria","authors":"Chenglong Ding, Zhidan Sun, Y. Andrew Wang, Han Zhou, Qing Huang, Wencheng Song","doi":"10.1002/ppap.202400244","DOIUrl":"https://doi.org/10.1002/ppap.202400244","url":null,"abstract":"ABSTRACT Dielectric barrier discharge (DBD) is a method that promotes wound healing. This study designed a flexible plasma pad with a polyimide film as the dielectric layer and patterned copper foil as the ground electrode. The flexible plasma pad was used to treat normal skin cells and bacteria at the wound site. Cell viability, intracellular and extracellular active substances, and Western blot analysis indicated that LTP can activate the PI3K/AKT/mTOR signaling pathway, decrease E‐Cadherin expression, and promote HaCaT cell proliferation. It exhibits antibacterial activity against Staphylococcus aureus and Escherichia coli . The results of this study suggest that this plasma pad is an efficient device for treating wounds and bacterial infections.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"22 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147904608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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