冷大气等离子处理介质对体外 HaCaT 和 HUVEC 细胞的影响

IF 2.9 3区 物理与天体物理 Q2 PHYSICS, APPLIED
Mingyan Chen, Junjin Chen, Tian Xie, Zheng Chen, Guimin Xu
{"title":"冷大气等离子处理介质对体外 HaCaT 和 HUVEC 细胞的影响","authors":"Mingyan Chen, Junjin Chen, Tian Xie, Zheng Chen, Guimin Xu","doi":"10.1002/ppap.202400132","DOIUrl":null,"url":null,"abstract":"Cold atmospheric plasma (CAP) is an emerging technology that can generate various reactive oxygen and nitrogen species (RONS) at room temperature and shows promising applications for skin wound healing. The effects of plasma‐treated medium (PTM) promoting cell proliferation have been verified, but the biological mechanisms involved are not well known. This study aims to assess the proliferation effect and mechanism induced by PTM on human keratinocyte cells (HaCaT) and human umbilical vein endothelial cells (HUVEC) in vitro. The results showed that the concentrations of H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> and NO<jats:sub>2</jats:sub><jats:sup>−</jats:sup> inside PTM increased in a time‐dependent manner as the atmospheric pressure plasma jet (APPJ) treatment time was prolonged. The biological outcomes were determined by cell counting kit, wound healing assay, flow cytometry, enzyme‐linked immunosorbent assay (ELISA), and western blot analysis. The cell viability and motility assays indicated that appropriate plasma conditions of short treatment time (15s‐, 30s‐ and 45s‐PTM) could promote cell proliferation, while long treatment time would inhibit cell proliferation (60s‐PTM). With an appropriate time of PTM treatment, the secretion of vascular endothelial growth factor‐α (VEGF‐α) and transforming growth factor alpha (TGF‐α) was promoted and the extracellular signal‐regulated kinase/serine‐threonine protein kinase pathways were activated, which induced HaCaT and HUVEC cell proliferation eventually. These behaviors of cells were mainly related to the enhancement of intracellular reactive oxygen species levels. These findings established a theoretical foundation for potential clinical applications of PTM in wound healing.","PeriodicalId":20135,"journal":{"name":"Plasma Processes and Polymers","volume":"8 1","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of cold atmospheric plasma‐treated medium on HaCaT and HUVEC cells in vitro\",\"authors\":\"Mingyan Chen, Junjin Chen, Tian Xie, Zheng Chen, Guimin Xu\",\"doi\":\"10.1002/ppap.202400132\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Cold atmospheric plasma (CAP) is an emerging technology that can generate various reactive oxygen and nitrogen species (RONS) at room temperature and shows promising applications for skin wound healing. The effects of plasma‐treated medium (PTM) promoting cell proliferation have been verified, but the biological mechanisms involved are not well known. This study aims to assess the proliferation effect and mechanism induced by PTM on human keratinocyte cells (HaCaT) and human umbilical vein endothelial cells (HUVEC) in vitro. The results showed that the concentrations of H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> and NO<jats:sub>2</jats:sub><jats:sup>−</jats:sup> inside PTM increased in a time‐dependent manner as the atmospheric pressure plasma jet (APPJ) treatment time was prolonged. The biological outcomes were determined by cell counting kit, wound healing assay, flow cytometry, enzyme‐linked immunosorbent assay (ELISA), and western blot analysis. The cell viability and motility assays indicated that appropriate plasma conditions of short treatment time (15s‐, 30s‐ and 45s‐PTM) could promote cell proliferation, while long treatment time would inhibit cell proliferation (60s‐PTM). With an appropriate time of PTM treatment, the secretion of vascular endothelial growth factor‐α (VEGF‐α) and transforming growth factor alpha (TGF‐α) was promoted and the extracellular signal‐regulated kinase/serine‐threonine protein kinase pathways were activated, which induced HaCaT and HUVEC cell proliferation eventually. These behaviors of cells were mainly related to the enhancement of intracellular reactive oxygen species levels. These findings established a theoretical foundation for potential clinical applications of PTM in wound healing.\",\"PeriodicalId\":20135,\"journal\":{\"name\":\"Plasma Processes and Polymers\",\"volume\":\"8 1\",\"pages\":\"\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plasma Processes and Polymers\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://doi.org/10.1002/ppap.202400132\",\"RegionNum\":3,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PHYSICS, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plasma Processes and Polymers","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1002/ppap.202400132","RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHYSICS, APPLIED","Score":null,"Total":0}
引用次数: 0

摘要

冷大气等离子体(CAP)是一种新兴技术,可在室温下产生各种活性氧和氮物种(RONS),在皮肤伤口愈合方面具有广阔的应用前景。等离子体处理介质(PTM)促进细胞增殖的效果已得到验证,但其中的生物学机制尚不清楚。本研究旨在评估 PTM 在体外诱导人角质细胞(HaCaT)和人脐静脉内皮细胞(HUVEC)增殖的效果和机制。结果表明,随着常压等离子体射流(APPJ)处理时间的延长,PTM 内 H2O2 和 NO2- 的浓度呈时间依赖性增加。生物学结果通过细胞计数试剂盒、伤口愈合试验、流式细胞术、酶联免疫吸附试验(ELISA)和免疫印迹分析进行测定。细胞活力和运动试验表明,适当的血浆条件下,短处理时间(15 秒、30 秒和 45 秒-PTM)可促进细胞增殖,而长处理时间(60 秒-PTM)则会抑制细胞增殖。适当的 PTM 处理时间可促进血管内皮生长因子-α(VEGF-α)和转化生长因子α(TGF-α)的分泌,激活细胞外信号调节激酶/丝氨酸-苏氨酸蛋白激酶通路,最终诱导 HaCaT 和 HUVEC 细胞增殖。细胞的这些行为主要与细胞内活性氧水平的提高有关。这些发现为 PTM 在伤口愈合中的潜在临床应用奠定了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of cold atmospheric plasma‐treated medium on HaCaT and HUVEC cells in vitro
Cold atmospheric plasma (CAP) is an emerging technology that can generate various reactive oxygen and nitrogen species (RONS) at room temperature and shows promising applications for skin wound healing. The effects of plasma‐treated medium (PTM) promoting cell proliferation have been verified, but the biological mechanisms involved are not well known. This study aims to assess the proliferation effect and mechanism induced by PTM on human keratinocyte cells (HaCaT) and human umbilical vein endothelial cells (HUVEC) in vitro. The results showed that the concentrations of H2O2 and NO2 inside PTM increased in a time‐dependent manner as the atmospheric pressure plasma jet (APPJ) treatment time was prolonged. The biological outcomes were determined by cell counting kit, wound healing assay, flow cytometry, enzyme‐linked immunosorbent assay (ELISA), and western blot analysis. The cell viability and motility assays indicated that appropriate plasma conditions of short treatment time (15s‐, 30s‐ and 45s‐PTM) could promote cell proliferation, while long treatment time would inhibit cell proliferation (60s‐PTM). With an appropriate time of PTM treatment, the secretion of vascular endothelial growth factor‐α (VEGF‐α) and transforming growth factor alpha (TGF‐α) was promoted and the extracellular signal‐regulated kinase/serine‐threonine protein kinase pathways were activated, which induced HaCaT and HUVEC cell proliferation eventually. These behaviors of cells were mainly related to the enhancement of intracellular reactive oxygen species levels. These findings established a theoretical foundation for potential clinical applications of PTM in wound healing.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Plasma Processes and Polymers
Plasma Processes and Polymers 物理-高分子科学
CiteScore
6.60
自引率
11.40%
发文量
150
审稿时长
3 months
期刊介绍: Plasma Processes & Polymers focuses on the interdisciplinary field of low temperature plasma science, covering both experimental and theoretical aspects of fundamental and applied research in materials science, physics, chemistry and engineering in the area of plasma sources and plasma-based treatments.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信