Photomedicine and laser surgery最新文献

{"title":"Determination of Optimum Operation Parameters for Low-Intensity Pulsed Ultrasound and Low-Level Laser Based Treatment to Induce Proliferation of Osteoblast and Fibroblast Cells.","authors":"Mehmet Emre Coskun,&nbsp;Kubra Acikalin Coskun,&nbsp;Yusuf Tutar","doi":"10.1089/pho.2017.4354","DOIUrl":"https://doi.org/10.1089/pho.2017.4354","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to determine the optimum operating parameters (pulse duration, energy levels, and application time) to promote induction of osteoblast and fibroblast cell proliferation and to maintain cell viability treated with low-intensity pulsed ultrasound (LIPUS) and low-level laser therapy (LLLT).</p><p><strong>Background data: </strong>The positive effects of LIPUS and LLLT on cellular activity have been reported in recent years. Comparisons between experimental parameters of previous studies are difficult because scientific studies reported frequencies and the duty cycles of LIPUS and wavelengths and doses of LLLT in a wide range of parameters. However, optimum amount of energy and optimum time exposure must be determined to induce bone and tissue cell proliferation for effective healing process and to avoid cell damage.</p><p><strong>Material and methods: </strong>Fibroblast and osteoblast cell cultures were irradiated with LIPUS (10-50% pulse and continuous mode at 1 and 3 MHz for 1, 3, and 5 min) and LLLT (4, 8, and 16 J at 50, 100, 200, 300, 400, and 500 mW). Cell cultures were analyzed using XTT assay.</p><p><strong>Results: </strong>For both cell types, LIPUS treatment with 10% pulse (1:9 duty cycle), 3 MHz, and for 1 min and LLLT treatment over 100 mV for 4, 8, and 16 J modalities contributed to the growth, and may help bone repair and tissue healing process optimally.</p><p><strong>Conclusions: </strong>Bio-stimulating effects of LLLT irradiation promote proliferation and maintain cell viability better than LIPUS treatment without causing thermal response for both cell types, and the therapeutic modality above 200 mV has maximum effectiveness.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 5","pages":"246-252"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4354","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35850566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectral Radiance of Protoporphyrin IX Fluorescence and Its Histopathological Implications in 5-Aminolevulinic Acid-Guided Surgery for Glioblastoma.","authors":"Takashi Yoneda, Naosuke Nonoguchi, Naokado Ikeda, Ryokichi Yagi, Shinji Kawabata, Motomasa Furuse, Yoshinobu Hirose, Hiroko Kuwabara, Yoji Tamura, Yoshinaga Kajimoto, Toshihiko Kuroiwa","doi":"10.1089/pho.2017.4384","DOIUrl":"10.1089/pho.2017.4384","url":null,"abstract":"<p><strong>Objective: </strong>This study is intended to objectively clarify the relationship between the fluorescence intensity emitted by protoporphyrin IX (PpIX), which is a metabolite of 5-aminolevulinic acid (ALA), and histological findings during glioblastoma surgery.</p><p><strong>Background: </strong>ALA is widely used for the intraoperative detection of tumors. There are several reports about the fluorescence of PpIX and the histological findings of tumors, but judgments about the fluorescence intensity depend largely on the subjective sense of each surgeon.</p><p><strong>Methods: </strong>We quantified the PpIX fluorescence intensity emitted from tissue specimens using a spectroradiometer and evaluated the relationship between a spectral radiance of 635 nm and the histopathological features of surgical specimens of glioblastoma. Surgical samples from glioblastoma patients consist of a strongly fluorescent area (SFA) or vaguely fluorescent area (VFA). Hematoxylin and eosin staining, immunohistochemical Ki-67, and CD31 staining were performed to evaluate the cell density, MIB-1 index, and vascularity, respectively. The fluorescence intensities of each sample were compared with each histopathological parameter.</p><p><strong>Results: </strong>Cell density, MIB-1 index, and total vascular area were significantly correlated with PpIX fluorescence radiance. 87.5% of SFA were judged to be tumor bulk consisting mostly of tumor cells and 12.5% peritumoral invaded brain. In the VFA, 100% of specimens were judged to be peritumoral invaded brain.</p><p><strong>Conclusions: </strong>ALA-induced PpIX fluorescence has quantitatively correlated well with histopathological malignant features both in SFA and VFA. These findings suggest that not only SFA but also VFA should be removed to the highest extent that does not cause neurological symptoms.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":" ","pages":"266-272"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39984302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Influence of Low-Power Diode Laser Irradiation Time on Human Red Blood Cells.","authors":"Amal Yousif Al-Yasiri","doi":"10.1089/pho.2017.4395","DOIUrl":"https://doi.org/10.1089/pho.2017.4395","url":null,"abstract":"<p><strong>Objective: </strong>The present study investigates whether the exposure to low-power diode laser induces denaturation in red blood cell (RBC) membrane protein composition, and determines the irradiation time for when denaturation of membrane protein process begins.</p><p><strong>Background: </strong>A low-energy laser has been used extensively in medical applications. Several studies indicated significant positive effects of laser therapy on biological systems. In contrast, other studies reported that laser induced unwanted changes in cell structure and biological systems. The present work studied the effect of irradiation time of low-power diode laser on the structure of membrane proteins of human RBCs.</p><p><strong>Materials and methods: </strong>The RBC suspension was divided into five equal aliquots. One aliquot served as control. The remaining four aliquots were exposed to low-power diode laser (wave length = 650 nm, power = 50 mW) for 10, 20, 30, and 40 min, respectively. After each given time, the percentage of denatured RBCs was calculated in each sample as described later.</p><p><strong>Results: </strong>Irradiation of RBCs by this laser for 20 min did not cause any change in membrane protein composition. While increasing the irradiation time to 30 min caused denaturation of membrane proteins, resulting in the formation of cross-bonding in a considerable number of RBCs, and the percentage of denatured cells increased in a dose-dependent manner to the irradiation.</p><p><strong>Conclusions: </strong>It can be concluded that the effect of low-power diode laser on RBC membrane protein structure depends on irradiation time.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 5","pages":"253-257"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35787683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current and Future Trends in Adipose Stem Cell Differentiation into Neuroglia.","authors":"Sajan George,&nbsp;Michael R Hamblin,&nbsp;Heidi Abrahamse","doi":"10.1089/pho.2017.4411","DOIUrl":"https://doi.org/10.1089/pho.2017.4411","url":null,"abstract":"<p><strong>Background: </strong>Neurological diseases and disorders pose a challenge for treatment and rehabilitation due to the limited capacity of the nervous system to repair itself. Adipose stem cells (ASCs) are more pliable than any adult stem cells and are capable of differentiating into non-mesodermal tissues, including neurons. Transdifferentiating ASCs to specific neuronal lineage cells enables us to deliver the right type of cells required for a replacement therapy into the nervous system.</p><p><strong>Methods: </strong>Several methodologies are being explored and tested to differentiate ASCs to functional neurons and glia with cellular factors and chemical compounds. However, none of these processes and prototypes has been wholly successful in changing the cellular structure and functional status of ASCs to become identical to neuroglial cells. In addition, successful integration and functional competence of these cells for use in clinical applications remain problematic. Photobiomodulation or low-level laser irradiation has been successfully applied to not only improve ASC viability and proliferation but has also shown promise as a possible enhancer of ASC differentiation.</p><p><strong>Conclusions: </strong>Studies have shown that photobiomodulation improves the use of stem cell transplantation for neurological applications. This review investigates current neuro-differentiation inducers and suitable methodologies, including photobiomodulation, utilizing ASCs for induction of differentiation into neuronal lineages.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 5","pages":"230-240"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35939970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation and Cancer: What Is the Truth?","authors":"Michael R Hamblin,&nbsp;Scott T Nelson,&nbsp;Justin R Strahan","doi":"10.1089/pho.2017.4401","DOIUrl":"https://doi.org/10.1089/pho.2017.4401","url":null,"abstract":"<p><strong>Background: </strong>Photobiomodulation (PBM) therapy is a rapidly growing approach to stimulate healing, reduce pain, increase athletic performance, and improve general wellness.</p><p><strong>Objective: </strong>Applying PBM therapy over the site of a tumor has been considered to be a contraindication. However, since another growing use of PBM therapy is to mitigate the side effects of cancer therapy, this short review seeks to critically examine the evidence of whether PBM therapy is beneficial or harmful in cancer patients.</p><p><strong>Materials and methods: </strong>PubMed and Google Scholar were searched.</p><p><strong>Results: </strong>Although there are a few articles suggesting that PBM therapy can be detrimental in animal models of tumors, there are also many articles that suggest the opposite and that light can directly damage the tumor, can potentiate other cancer therapies, and can stimulate the host immune system. Moreover, there are two clinical trials showing increased survival in cancer patients who received PBM therapy.</p><p><strong>Conclusions: </strong>PBM therapy may have benefits in cancer patients and should be further investigated.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 5","pages":"241-245"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4401","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35849971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aging Is a Sticky Business.","authors":"Andrei P Sommer","doi":"10.1089/pho.2017.4393","DOIUrl":"https://doi.org/10.1089/pho.2017.4393","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this work is to put forward a mechanism by which low-level light [red-to near infrared (NIR) laser or light emitting diodes (LED)] is instrumental in the process of accelerating the healing of wounds.</p><p><strong>Background data: </strong>Interaction modalities of low-level light with oxidatively stressed cells and tissues are the focus of intense research efforts. Several models of the light/cell-interaction mechanism have been proposed. In the most popular model, cytochrome c oxidase is believed to play the role of the principal acceptor for red-to NIR photons.</p><p><strong>Methods: </strong>Using as an illustrative example the successful LED treatment of an edematous limb ulcer, the results of recent in vitro tests and complementary laboratory experiments are presented and discussed.</p><p><strong>Results: </strong>The most plausible mechanism of biostimulatory effect of red-to NIR light consists of its impact on the nanoscopic interfacial water layers in mitochondria and the extracellular matrix (ECM) where mitochondrial reactive oxygen species (ROS) induce an increase in the viscosity of the water layers bound to the predominantly hydrophilic surfaces in the intramitochondrial space as well as the ECM, where the process progressively propagates with age. The biostimulatory effect of red-to NIR light consists of counteracting the ROS-induced elevation of interfacial water viscosities, thereby instantly restoring the normal mitochondrial function, including the synthesis of adenosine triphosphate (ATP) by the rotary motor (ATP synthase).</p><p><strong>Conclusions: </strong>An understanding of the mechanism of interaction of red-to NIR light with mitochondria, cells, and tissues safeguards progress in the field of low-level light therapy (LLLT) and puts us in the position to design better therapies.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 5","pages":"284-286"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4393","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35939969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioluminescence Analysis of Antibacterial Photodynamic Therapy Using Methylene Blue Mediated by Low-Intensity Level Laser Against Cariogenic Biofilms.","authors":"Fernando Luis Esteban Florez,&nbsp;Morgana Regina Mendonça de Oliveira,&nbsp;Osmir Batista de Oliveira Júnior,&nbsp;Rochelle Denise Hiers,&nbsp;Sharukh Soli Khajotia,&nbsp;Hermes Pretel","doi":"10.1089/pho.2017.4326","DOIUrl":"https://doi.org/10.1089/pho.2017.4326","url":null,"abstract":"<p><strong>Objective: </strong>A non-destructive and real-time bioluminescence (BL) assay was used to determine the utility of antimicrobial photodynamic therapy (aPDT) treatments mediated by methylene blue (MB) and laser irradiation (LI) against intact biofilms that are capable of producing caries (cariogenic). The efficacy of antibacterial photodynamic treatments has been currently determined by using either viable colony counts (VCC) or metabolic assays (Alamar Blue) that were demonstrated to have critical limitations when used on microcolony-forming bacteria such as Streptococcus mutans.</p><p><strong>Materials and methods: </strong>Resin composite specimens were fabricated, wet-polished, ultraviolet-sterilized, and stored in water (72 h). S. mutans (strain JM10) biofilms were grown [24 h; 0.65 × THY with 0.1% (w/v) sucrose] on the surfaces of sterile specimens. Antibacterial treatments were performed by using MB (0.0005% and 0.001%) with or without LI (660 ± 10 nm, 6 J/cm²). Specimens treated with chlorhexidine gluconate served as the negative control group. The efficacy of aPDT treatments was determined in terms of BL for intact biofilms and VCC for sonicated bacteria.</p><p><strong>Results: </strong>BL results were corrected by using the Greenhouse-Geisser method and were analyzed with repeated-measures analysis of variance and post hoc Bonferroni test (α = 0.05). VCC results were analyzed by using Kruskal-Wallis and Dunn's multiple-comparisons post hoc tests (α = 0.05). Our findings demonstrated that experimental treatments significantly decreased the viability of S. mutans biofilms (p < 0.05). Moderate reductions in cellular viability were observed on biofilms subjected to aPDT treatments.</p><p><strong>Conclusions: </strong>This study demonstrated that aPDT has promising potential to be used as an additional method to control oral cariogenic biofilms.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 5","pages":"258-265"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4326","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35720837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healing Tumors with Light: Science Fiction or the Future of Medicine?","authors":"Praveen R Arany","doi":"10.1089/pho.2018.4457","DOIUrl":"10.1089/pho.2018.4457","url":null,"abstract":"","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 5","pages":"227-229"},"PeriodicalIF":0.0,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36069576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Various Pretreatments on the Bond Strength of Denture Teeth to Denture Base Resins.","authors":"Fatih Sari,&nbsp;Ozlem Ustun,&nbsp;Omer Kirmali","doi":"10.1089/pho.2017.4408","DOIUrl":"https://doi.org/10.1089/pho.2017.4408","url":null,"abstract":"<p><strong>Objective: </strong>This in vitro study evaluated the shear bond strength (SBS) of heat-cured denture base resin (PMMA) to acrylic resin teeth treated with different pretreatments, especially laser irradiation of different powers (1-4 W).</p><p><strong>Materials and methods: </strong>The acrylic resin teeth were separated into seven groups (n = 10) for the following different pretreatments: control group (no surface treatment) (G1), grinding with a tungsten carbide bur (G2), sandblasting (G3), and erbium, chromium: yttrium, scandium, gallium, garnet (Er,Cr:YSGG) laser irradiation at 1 W, 2 W, 3 W, 4 W output powers in (G4-7), respectively. Test specimens were produced according to the PMMA manufacturers' instructions and were subjected to a SBS test at a crosshead speed of 1 mm/min until fracture. Debonded surfaces were evaluated by a stereomicroscope for the type of failure. SEM (scanning electron microscope) analyses were done to estimate the surface changes of the acrylic resin teeth. The data were submitted using a one-way ANOVA and post hoc Tukey-Kramer multiple comparison tests (p = 0.05).</p><p><strong>Results: </strong>The highest bond strength was obtained in G3, and similar SBS values were considered in other groups, and no significant differences were found among the surface treatments and the control group (p < 0.05). All groups had a high percentage of adhesive failures.</p><p><strong>Conclusions: </strong>Laser irradiations promote surface topography alterations. However laser irradiation of the adhesive surface was found ineffective. The SBS of acrylic resin teeth to a PMMA denture base material is independent of the surface pretreatments Er,Cr:YSGG laser irradiation, sandblasting, and grinding with a carbide bur. All the surface treatments provided a similar bond between the acrylic denture base and the teeth.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 4","pages":"214-220"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4408","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36007347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photons Harmony for Cell Communication.","authors":"Reza Fekrazad","doi":"10.1089/pho.2018.4451","DOIUrl":"10.1089/pho.2018.4451","url":null,"abstract":"","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 4","pages":"177-178"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36007346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}