In Vitro Influence of Low-Power Diode Laser Irradiation Time on Human Red Blood Cells.

Q2 Medicine

Photomedicine and laser surgery Pub Date : 2018-05-01 Epub Date: 2018-02-02 DOI:10.1089/pho.2017.4395

Amal Yousif Al-Yasiri

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引用次数: 3

Abstract

Objective: The present study investigates whether the exposure to low-power diode laser induces denaturation in red blood cell (RBC) membrane protein composition, and determines the irradiation time for when denaturation of membrane protein process begins.

Background: A low-energy laser has been used extensively in medical applications. Several studies indicated significant positive effects of laser therapy on biological systems. In contrast, other studies reported that laser induced unwanted changes in cell structure and biological systems. The present work studied the effect of irradiation time of low-power diode laser on the structure of membrane proteins of human RBCs.

Materials and methods: The RBC suspension was divided into five equal aliquots. One aliquot served as control. The remaining four aliquots were exposed to low-power diode laser (wave length = 650 nm, power = 50 mW) for 10, 20, 30, and 40 min, respectively. After each given time, the percentage of denatured RBCs was calculated in each sample as described later.

Results: Irradiation of RBCs by this laser for 20 min did not cause any change in membrane protein composition. While increasing the irradiation time to 30 min caused denaturation of membrane proteins, resulting in the formation of cross-bonding in a considerable number of RBCs, and the percentage of denatured cells increased in a dose-dependent manner to the irradiation.

Conclusions: It can be concluded that the effect of low-power diode laser on RBC membrane protein structure depends on irradiation time.

查看原文本刊更多论文

低功率二极管激光辐照时间对人红细胞的体外影响。

目的:研究低功率二极管激光照射是否会引起红细胞膜蛋白组成的变性，并确定膜蛋白变性开始的照射时间。背景:低能量激光器在医学上得到了广泛的应用。一些研究表明激光治疗对生物系统有显著的积极作用。相比之下，其他研究报告说，激光诱导细胞结构和生物系统的不必要的变化。本文研究了低功率二极管激光照射时间对人红细胞膜蛋白结构的影响。材料和方法:将红细胞悬液分成5等份。其中一组作为对照。其余四组分别暴露于低功率二极管激光(波长为650 nm，功率为50 mW)下10、20、30和40分钟。在每个给定时间后，按后面描述的方法计算每个样品中变性红细胞的百分比。结果:该激光照射红细胞20 min未引起膜蛋白组成的变化。而将照射时间增加至30min，引起膜蛋白变性，导致相当数量的红细胞形成交键，变性细胞百分比随照射量的增加呈剂量依赖性。结论:低功率二极管激光对红细胞膜蛋白结构的影响与照射时间有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photomedicine and laser surgery 医学-外科

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Photobiomodulation, Photomedicine, and Laser Surgery (formerly Photomedicine and Laser Surgery) is the essential journal for cutting-edge advances and research in phototherapy, low-level laser therapy (LLLT), and laser medicine and surgery. The Journal delivers basic and clinical findings and procedures to improve the knowledge and application of these techniques in medicine.