Photomedicine and laser surgery最新文献

{"title":"Antibacterial and Smear Layer Removal Efficacy of Er:YAG Laser Irradiation by Photon-Induced Photoacoustic Streaming in Primary Molar Root Canals: A Preliminary Study.","authors":"Emre Korkut,&nbsp;Emrah Torlak,&nbsp;Onur Gezgin,&nbsp;Hazal Özer,&nbsp;Yağmur Şener","doi":"10.1089/pho.2017.4369","DOIUrl":"https://doi.org/10.1089/pho.2017.4369","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to compare antibacterial and smear layer removal efficacy of the erbium:yttrium-aluminium-garnet (Er:YAG) laser irradiation by photon-induced photoacoustic streaming (PIPS), neodymium:yttrium-aluminium-garnet (Nd:YAG), and diode lasers and conventional irrigation agent in primary molar root canals.</p><p><strong>Background: </strong>The selection, application, and activation of the irrigant are important factors affecting the success of the endodontic treatment.</p><p><strong>Materials and methods: </strong>Distal root canals of human primary mandibular molar teeth used for antimicrobial efficacy evaluation were first inoculated with Enterococcus faecalis (n = 25). Smear layer removal efficacy was evaluated on the noninoculated specimens (n = 20). The root canals in the first treatment groups were irrigated with a conventional technique using 5% sodium hypochlorite (NaOCl). In the laser groups, the root canals were irradiated with either Nd:YAG (1064 nm) or diode lasers (940 nm) followed by NaOCl irrigation agent. In the fourth groups, the NaOCl irrigation agent was activated with an Er:YAG laser (2940 nm) by PIPS tip using nonablative settings. Antibacterial efficacy was determined by viable counts of E. faecalis after treatments. The treated root canals were examined by scanning electron microscopy to determine the smear layer removal efficacy of treatments.</p><p><strong>Results: </strong>Statistically significant (p < 0.05) reductions in the number of E. faecalis were achieved in Nd:YAG and diode laser groups and Er:YAG laser by PIPS-activated irrigation groups compared to the NaOCl group. Er:YAG laser by PIPS-activated irrigation resulted in more cleaning of the root canal walls and a higher quantity of open tubules.</p><p><strong>Conclusions: </strong>These findings suggest that Er:YAG laser by PIPS-activated irrigation can be considered as an effective irrigant agitation technique in pediatric endodontics.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 9","pages":"480-486"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4369","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36224691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term Effect of Pulsed Nd:YAG Laser in the Treatment of Children with Juvenile Rheumatoid Arthritis: A Randomized Controlled Trial.","authors":"Shamekh Mohamed El-Shamy,&nbsp;Mohamed Salaheldien Mohamed Alayat,&nbsp;Abdelgalil Allam Abdelgalil,&nbsp;Mansour Abdullah Alshehri","doi":"10.1089/pho.2018.4444","DOIUrl":"https://doi.org/10.1089/pho.2018.4444","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to evaluate the long-term impact of a pulsed neodymium-doped yttrium aluminum garnet (Nd:YAG) laser [high-intensity laser therapy (HILT)] in the treatment of juvenile rheumatoid arthritis (JRA).</p><p><strong>Materials and methods: </strong>A sample of 30 children participated in this study (15 in the laser group and 15 in the placebo group), with a mean age of 10.53 ± 1.25 years. Children who were randomly assigned to the laser group received HILT thrice per week for 4 weeks, plus the exercise program. HILT scanned each knee with 600 J in two phases and 15 J to 10 points for a total of 750 J for each knee. The placebo laser group received placebo HILT plus the same exercise program. The outcomes measured in this study were the pain level by the visual analog scale (VAS) and gait parameters by the GAITRite^® system. Statistical analysis was performed by ANOVA with repeated measures to compare the differences between the baseline, post-treatment, and 12-week follow-up measurements for both groups. The level of significance was set at p < 0.05.</p><p><strong>Results: </strong>The VAS results significantly decreased post-treatment in the laser group relative to the placebo group and were still improved at the 12-week follow-up. Gait parameters significantly increased in the laser group after 4 weeks of treatment and after 12 weeks compared to the placebo group.</p><p><strong>Conclusions: </strong>HILT, when combined with an exercise program, appears to be more effective in children with JRA than a placebo laser procedure with exercises.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"445-451"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.4444","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36319838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shear Bond Strength of Nanohybrid Composite to the Resin Matrix Ceramics After Different Surface Treatments.","authors":"Ersan Çelik,&nbsp;Sezgi Cinel Sahin,&nbsp;Doğu Ömür Dede","doi":"10.1089/pho.2018.4467","DOIUrl":"https://doi.org/10.1089/pho.2018.4467","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to evaluate the effects of different surface treatments on the shear bond strength (SBS) of a composite resin to the various resin matrix ceramics (RMC).</p><p><strong>Background: </strong>Clinical applications of physical and chemical surface conditioning methods may be required for RMC to optimize the adhesion of additional resin materials.</p><p><strong>Materials and methods: </strong>Ninety-nine RMC specimens (6 × 6 × 2 mm) were prepared from a resin nanoceramic (RNC), a polymer-infiltrated hybrid ceramic (PIHC), and a flexible hybrid ceramic (FHC) block with a cutting machine under copious water. All specimens divided randomly into three surface treatment subgroups (n = 11): (1) untreated (Cnt), (2) tribochemical silica coated (Tbc), and (3) neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiated with 3 W (150 mJ/pulse, 20 Hz) for 20 sec. A nanohybrid composite resin was layered with a disc-shape mold (2 × 3 mm) onto the ceramics and submitted to thermal cycling (3000 cycles, 5-55°C). The SBS test of specimens was performed using a universal testing machine. Data were statistically analyzed with two-way analysis of variance (ANOVA) and Tukey HSD test (α = 0.05).</p><p><strong>Results: </strong>The type of RMC, surface treatments, and their interactions was statistically significant on SBS, according to the ANOVA (p < 0.05). Both the Tbc and Lsr surface treatments significantly increased the SBS values of FHC (p < 0.001), while significant increases were observed only for Lsr0applied RNC and Tbc applied PIHC groups (p < 0.001).</p><p><strong>Conclusions: </strong>The tribochemical silica coating and laser irradiation techniques may be suitable for improving the surface topography of specific types of RMC. While the same surface treatment technique may not provide similar successful results for all types of RMC, it is recommended to choose the most effective and reliable technique for clinical application.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"424-430"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.4467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36321214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration of Surface Glycoproteins After Photodynamic Therapy.","authors":"Roberta Kelly de Faria Souza,&nbsp;Isabel Chaves Silva Carvalho,&nbsp;Carolina Genúncio da Cunha Menezes Costa,&nbsp;Newton Soares da Silva,&nbsp;Cristina Pacheco-Soares","doi":"10.1089/pho.2018.4464","DOIUrl":"https://doi.org/10.1089/pho.2018.4464","url":null,"abstract":"<p><strong>Background: </strong>Cell membranes have been identified as an important intracellular cancer treatment target, since the glycoconjugates present on the cell surface are involved in numerous cell functions. Photodynamic therapy (PDT) is a therapeutic modality employed in the treatment of tumors that uses visible light to activate a photosensitizer.</p><p><strong>Objective: </strong>This study analyzed the expression of surface carbohydrates after PDT with two different photosensitizers, 5-aminolevulinic acid (ALA) and Photosan-3.</p><p><strong>Methods: </strong>Mice were injected subcutaneously with 2 × 10⁵ B16 cells. After 7-10 days, the presence of a tumor with a diameter of 3.6 mm was observed. Photosan-3^® and 5-aminolevulinic acid-ALA were used in the PDT treatment. Control animals (not submitted to either laser treatment or photosensitizer injection) and treated animals were euthanized 15 days post-treatment. The tumors were irradiated with a red diode laser, λ = 655 nm, energy density of 10 J.cm^-2, and power density of 45 mW.cm^-2. After 2 weeks of treatment with PDT, the mice were euthanized, the tumors were collected, and the cell surfaces were labeled with lectins concanavalin A (ConA) and wheat germ agglutinin (WGA).</p><p><strong>Results: </strong>Fluorescence microscopy analysis of the cell surfaces with lectins ConA and WGA showed the presence of α-mannose and α-glucose.</p><p><strong>Conclusions: </strong>The combined effects of either Photosan-3 or ALA and red laser light on melanoma suggest an inhibitory glycosylation action from PDT on the surface of B16-F10 cells.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"452-456"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.4464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36321217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is Photodynamic Therapy Resistance a Special Case of Photobiomodulation?","authors":"Brendan J Quirk,&nbsp;Albert W Girotti,&nbsp;Harry T Whelan","doi":"10.1089/pho.2018.4501","DOIUrl":"https://doi.org/10.1089/pho.2018.4501","url":null,"abstract":"","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"397-398"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.4501","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36379868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation, Photomedicine, and Laser Surgery: A New Leap Forward Into the Light for the 21^st Century.","authors":"Michael R Hamblin","doi":"10.1089/pho.2018.29011.mrh","DOIUrl":"https://doi.org/10.1089/pho.2018.29011.mrh","url":null,"abstract":"","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"395-396"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.29011.mrh","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36379867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Photobiomodulation Therapy on Gingivitis Induced by Multi-Bracket Appliances: A Split-Mouth Randomized Controlled Trial.","authors":"Steffen Stein,&nbsp;Michael Schauseil,&nbsp;Andreas Hellak,&nbsp;Heike Korbmacher-Steiner,&nbsp;Andreas Braun","doi":"10.1089/pho.2017.4404","DOIUrl":"https://doi.org/10.1089/pho.2017.4404","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this split-mouth trial was to investigate the influence of photobiomodulation therapy (PBMT) on adjuvant treatment of gingivitis induced by multi-bracket appliances, after bracket debonding and professional tooth cleaning.</p><p><strong>Materials and methods: </strong>Thirteen patients (mean age 16.15 years; standard deviation ±2.12 years) who had completed active orthodontic treatment with fixed orthodontic appliances in an orthodontic clinic were included on a randomized basis. At time point T0, after bracket debonding and professional tooth cleaning, the papilla bleeding index (PBI) and bleeding on probing (BOP) were assessed in the upper jaw by the blinded investigator (M.S.), who was not aware at any time of which quadrant received PBMT. The study was based on a patient-blinded split-mouth design. In each patient, PBMT was administered by a practitioner (S.S.) in one upper quadrant (wavelength: 660 nm; Power: 100 mW; Power density: 100 mW/cm²; Energy density per application point = 2 J/cm²; Energy per application point = 2 J; Total dose = 52 J/cm²; Total energy = 52 J; Irradiation time: 26 × 20 sec), while the other upper quadrant received a simulated laser application with the laser system turned off. Randomized equal allocation of the sides was accomplished. The second PBI and BOP assessment followed 4-6 days after laser irradiation (T1) by M.S.</p><p><strong>Results: </strong>No statistical differences were observed between the sides with regard to PBI and BOP values at T0 (p > 0.05). The PBI and BOP values decreased significantly between T0 and T1 on both sides (p < 0.05). At T1, the PBI and BOP values were significantly lower in the laser side in comparison with the control side (p < 0.05).</p><p><strong>Conclusions: </strong>On the basis of these results and study parameters, adjuvant PBMT is able to accelerate the healing process in patients with gingivitis induced by multi-bracket appliances.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"399-405"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2017.4404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36111312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Er,Cr:YSGG Laser and Ascorbic Acid on the Bond Strength and Microleakage of Bleached Enamel Surface.","authors":"Fahad Alkhudhairy,&nbsp;Abdulaziz AlKheraif,&nbsp;Mohammed Bin-Shuwaish,&nbsp;Sulieman Al-Johany,&nbsp;Mustafa Naseem,&nbsp;Fahim Vohra","doi":"10.1089/pho.2018.4437","DOIUrl":"https://doi.org/10.1089/pho.2018.4437","url":null,"abstract":"<p><strong>Background: </strong>The available evidence of Er,Cr:YSGG lasers on bleached enamel is limited and controversial. It is assumed that Er,Cr:YSGG laser will be effective in reversing the effect of bleaching on adhesive bond integrity of enamel to resin composite.</p><p><strong>Objective: </strong>The study aimed to evaluate the efficacy of Er,Cr:YSGG laser on bond strength and microleakage of bleached enamel.</p><p><strong>Materials and methods: </strong>From the 80 samples, 60 samples were bleached (40% hydrogen peroxide) and divided into three subgroups. Twenty bleached samples in each subgroup were exposed to 10% sodium ascorbate (Gp A), Er,Cr:YSGG laser (50 Hz and 4.5 W) (Gp L), and no treatment (Gp B), respectively. Twenty samples were not bleached (Control-C). Using etch and rinse technique, resin composite was built-up on enamel and photopolymerized. Ten specimens in each group were tested for shear bond strength at a crosshead speed of 1 mm/min. Ten specimens in each group were tested for microleakage using 2% methylene blue. Data were analyzed using analysis of variance (ANOVA) and Tukey post hoc multiple comparisons test.</p><p><strong>Results: </strong>The mean bond strength of specimens in group L (30.83 ± 1.7000) was comparable with group A (31.38 ± 2.03) and was significantly higher than bleached specimens (9.49 ± 0.86) (p < 0.001). The highest mean microleakage values were observed in bleached group (630.32 ± 156.58). The mean microleakage among control (36.66 ± 27.33) and laser group specimens (55.32 ± 34.98) was comparable (p > 0.05).</p><p><strong>Conclusions: </strong>Treatment of bleached enamel with erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser showed a significant reversal effect on the enamel bond strength and microleakage scores.</p><p><strong>Clinical significance: </strong>Er,Cr:YSGG laser (50 Hz, 4.5 W) treatment has the potential to restore the bond strength of bleached enamel to resin composite.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"431-438"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.4437","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36032441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effects of Photobiomodulation on Partial Osteotomy in Streptozotocin-Induced Diabetes in Rats.","authors":"Ataroalsadat Mostafavinia,&nbsp;Reza Masteri Farahani,&nbsp;Mohammad-Amin Abdollahifar,&nbsp;Mahdi Ghatrehsamani,&nbsp;Seyed Kamran Ghoreishi,&nbsp;Behnam Hajihossainlou,&nbsp;Sufan Chien,&nbsp;Sara Dadras,&nbsp;Fatemehalsadat Rezaei,&nbsp;Mohammad Bayat","doi":"10.1089/pho.2018.4438","DOIUrl":"https://doi.org/10.1089/pho.2018.4438","url":null,"abstract":"<p><strong>Objective: </strong>We examined the effects of photobiomodulation (PBM) on stereological parameters, and gene expression of Runt-related transcription factor 2 (RUNX2), osteocalcin, and receptor activator of nuclear factor kappa-B ligand (RANKL) in repairing tissue of tibial bone defect in streptozotocin (STZ)-induced type 1 diabetes mellitus (TIDM) in rats during catabolic response of fracture healing.</p><p><strong>Background data: </strong>There were conflicting results regarding the efficacy of PBM on bone healing process in healthy and diabetic animals.</p><p><strong>Materials and methods: </strong>Forty-eight rats have been distributed into four groups: group 1 (healthy control, no TIDM and no PBM), group 2 (healthy test, no TIDM and PBM), group 3 (diabetic control, TIDM and no PBM), and group 4 (diabetic test, no TIDM and PBM). TIDM was induced in the groups 3 and 4. A partial bone defect in tibia was made in all groups. The bone defects of groups second and fourth were irradiated by a laser (890 nm, 80 Hz, 1.5 J/cm²). Thirty days after the surgery, all bone defects were extracted and were submitted to stereological examination and real-time polymerase chain reaction (RT-PCR).</p><p><strong>Results: </strong>PBM significantly increased volumes of total callus, total bone, bone marrow, trabecular bone, and cortical bone, and the numbers of osteocytes and osteoblasts of callus in TIDM rats compared to those of callus in diabetic control. In addition, TIDM increased RUNX2, and osteocalcin in callus of tibial bone defect compared to healthy group. PBM significantly decreased osteocalcin gene expression in TIDM rats.</p><p><strong>Conclusions: </strong>PBM significantly increased many stereological parameters of bone repair in an STZ-induced TIDM during catabolic response of fracture healing. Further RT-PCR test demonstrated that bone repair was modulated in diabetic rats during catabolic response of fracture healing by significant increase in mRNA expression of RUNX2, and osteocalcin compared to healthy control rats. PBM also decreased osteocalcin mRNA expression in TIDM rats.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"406-414"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.4438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36179754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Photobiomodulation on Degranulation and Number of Mast Cells and Wound Strength in Skin Wound Healing of Streptozotocin-Induced Diabetic Rats.","authors":"Mohammad Bagheri,&nbsp;Abdollah Amini,&nbsp;Mohammad-Amin Abdollahifar,&nbsp;Seyed Kamran Ghoreishi,&nbsp;Abbas Piryaei,&nbsp;Ramin Pouriran,&nbsp;Sufan Chien,&nbsp;Sara Dadras,&nbsp;Fatemehalsadat Rezaei,&nbsp;Mohammad Bayat","doi":"10.1089/pho.2018.4453","DOIUrl":"https://doi.org/10.1089/pho.2018.4453","url":null,"abstract":"<p><strong>Background: </strong>A lack of effective treatments still exists for patients suffering from diabetes mellitus. Photobiomodulation is proved as a beneficial therapeutic modality for wounds.</p><p><strong>Objective: </strong>The aim of this study is to examine the effect of degranulation of mast cells and total number of mast cells in the remodeling step of an ischemic model of wound healing under the influence of photobiomodulation and conditioned medium (CM) from human bone marrow-derived mesenchymal stem cells (hBM-MSCs-CM), or CM, administered alone and or in combination.</p><p><strong>Materials and methods: </strong>Initially, type 1 diabetes mellitus was induced in 72 male adult rats. Then, after a month, one incision was made on the back of each rat. Subsequently, the rats were divided into four groups. The first group was considered as the control (placebo) group, the second group received CM, the third group received photobiomodulation, and the fourth group received photobiomodulation+CM. On days 4, 7, and 15, samples were extracted from the wound for histological and tensiometric examinations. The total number of mast cells, including the three types of mast cells, was counted by the stereological methods. The tensiometric properties of the repairing tissue were examined.</p><p><strong>Results: </strong>The administration of photobiomodulation and CM, alone or in combination, significantly increased the tensiometric properties within the healing wounds. Histologically, photobiomodulation+CM, CM, and photobiomodulation groups showed a significant decrease in the three types of mast cells and in the total number of mast cells compared with the control group on day 15.</p><p><strong>Conclusions: </strong>We conclude that photobiomodulation and CM alone and or in combination significantly accelerated the healing process in a rat with a diabetic and ischemic wound, and significantly decreased the total number of mast cells and degranulation of mast cells. We suggest that the increased number of type 2 mast cells in the control group adversely affected the tensiometric properties of wounds in this group.</p>","PeriodicalId":20117,"journal":{"name":"Photomedicine and laser surgery","volume":"36 8","pages":"415-423"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/pho.2018.4453","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36309774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}